Abstract：
BACKGROUND:The therapeutic efficacy of human mesenchymal stem cells (hMSCs) for the treatment of hypoxic-ischemic diseases is closely related to level of hypoxia in the damaged tissues. To elucidate the potential therapeutic applications and limitations of hMSCs derived from human umbilical cords, the effects of hypoxia on the morphology and proliferation of hMSCs were analyzed. RESULTS:After treatment with DFO and CoCl₂, hMSCs were elongated, and adjacent cells were no longer in close contact. In addition, vacuole-like structures were observed within the cytoplasm; the rough endoplasmic reticulum expanded, and expanded ridges were observed in mitochondria. In addition, DFO and CoCl₂ treatments for 48 h significantly inhibited hMSCs proliferation in a concentration-dependent manner (P < 0.05). This treatment also increased the number of cells in G0/G1 phase and decreased those in G2/S/M phase. CONCLUSIONS:The hypoxia-mimetic agents, DFO and CoCl₂, alter umbilical cord-derived hMSCs morphology and inhibit their proliferation through influencing the cell cycle.
journal_name
BMC Cell Bioljournal_title
BMC cell biologyauthors
Zeng HL,Zhong Q,Qin YL,Bu QQ,Han XA,Jia HT,Liu HWdoi
10.1186/1471-2121-12-32subject
Has Abstractpub_date
2011-08-09 00:00:00pages
32issn
1471-2121pii
1471-2121-12-32journal_volume
12pub_type
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