Abstract:
BACKGROUND:Filamin (FLN) and non-muscle alpha-actinin are members of a family of F-actin cross-linking proteins that utilize Calponin Homology domains (CH-domain) for actin binding. Although these two proteins have been extensively characterized, little is known about what regulates their binding to F-actin filaments in the cell. RESULTS:We have constructed fusion proteins consisting of green fluorescent protein (GFP) with either the entire cross-linking protein or its actin-binding domain (ABD) and examined the localization of these fluorescent proteins in living cells under a variety of conditions. The full-length fusion proteins, but not the ABD's complemented the defects of cells lacking both endogenous proteins indicating that they are functional. The localization patterns of filamin (GFP-FLN) and alpha-actinin (GFP-alphaA) were overlapping but distinct. GFP-FLN localized to the peripheral cell cortex as well as to new pseudopods of unpolarized cells, but was observed to localize to the rear of polarized cells during cAMP and folate chemotaxis. GFP-alphaA was enriched in new pseudopods and at the front of polarized cells, but in all cases was absent from the peripheral cortex. Although both proteins appear to be involved in macropinocytosis, the association time of the GFP-probes with the internalized macropinosome differed. Surprisingly, the localization of the GFP-actin-binding domain fusion proteins precisely reflected that of their respective full length constructs, indicating that the localization of the protein was determined by the actin-binding domain alone. When expressed in a cell line lacking both filamin and alpha-actinin, the probes maintain their distinct localization patterns suggesting that they are not functionally redundant. CONCLUSION:These observations strongly suggest that the regulation of the binding of these proteins to actin filaments is built into the actin-binding domains. We suggest that different actin binding domains have different affinities for F-actin filaments in functionally distinct regions of the cytoskeleton.
journal_name
BMC Cell Bioljournal_title
BMC cell biologyauthors
Washington RW,Knecht DAdoi
10.1186/1471-2121-9-10subject
Has Abstractpub_date
2008-02-13 00:00:00pages
10issn
1471-2121pii
1471-2121-9-10journal_volume
9pub_type
杂志文章相关文献
BMC CELL BIOLOGY文献大全abstract:BACKGROUND:In cytokinesis, when the cleavage furrow has been formed, the two centrioles in each daughter cell separate. It has been suggested that the centrioles facilitate and regulate cytokinesis to some extent. It has been postulated that termination of cytokinesis (abscission) depends on the migration of a centriol...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-11-34
更新日期:2010-05-21 00:00:00
abstract:BACKGROUND:Cellular senescence plays important roles in the aging process of complex organisms, in tumor suppression and in response to stress. Several markers can be used to identify senescent cells, of which the most widely used is the senescence-associated β-galactosidase (SABG) activity. The main advantage of SABG ...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-12-16
更新日期:2011-04-15 00:00:00
abstract:BACKGROUND:The nucleolus is a multi-domain enriched with proteins involved in ribosome biogenesis, cell cycle and apoptosis control, viral replication and differentiation of stem cells. Several authors have suggested a role for the nucleolus also in malignant transformation. We have recently demonstrated that under spe...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/s12860-015-0073-5
更新日期:2015-11-17 00:00:00
abstract:BACKGROUND:The Scar/WAVE family of proteins mediates signals to actin assembly by direct activation of the Arp2/3 complex. These proteins have been characterised as major regulators of lamellipodia formation downstream of Rac activation and as members of large protein complexes. RESULTS:We have investigated the intera...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-6-11
更新日期:2005-03-07 00:00:00
abstract:BACKGROUND:The MAL2 gene, encoding a four-transmembrane protein of the MAL family, is amplified and overexpressed in breast and other cancers, yet the significance of this is unknown. MAL-like proteins have trafficking functions, but their molecular roles are largely obscure, partly due to a lack of known binding partn...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-10-7
更新日期:2009-01-28 00:00:00
abstract:BACKGROUND:Neuronal ceroid lipofuscinoses (NCLs) are collectively the most common type of recessively inherited childhood encephalopathies. The most severe form of NCL, infantile neuronal ceroid lipofuscinosis (INCL), is caused by mutations in the CLN1 gene, resulting in a deficiency of the lysosomal enzyme, palmitoyl ...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-8-22
更新日期:2007-06-12 00:00:00
abstract:BACKGROUND:Spermatogenesis is comprised of a series of highly regulated developmental changes that transform the precursor germ cell into a highly specialized spermatozoon. The last phase of spermatogenesis, termed spermiogenesis, involves dramatic morphological change including formation of the acrosome, elongation an...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-9-9
更新日期:2008-01-31 00:00:00
abstract:BACKGROUND:We have previously obtained a clonal population of cells from human foreskin that is able to differentiate into mesodermal, ectodermal and endodermal progenies. It is of great interest to know whether these cells could be further differentiated into functional insulin-producing cells. RESULTS:Sixty-one sing...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-11-46
更新日期:2010-06-25 00:00:00
abstract:BACKGROUND:The present review summarizes current knowledge about microparticles (MPs) and provides a systematic overview of last 20 years of research on circulating MPs, with particular focus on their clinical relevance. RESULTS:MPs are a heterogeneous population of cell-derived vesicles, with sizes ranging between 50...
journal_title:BMC cell biology
pub_type: 杂志文章,评审
doi:10.1186/1471-2121-14-23
更新日期:2013-04-22 00:00:00
abstract::Shroom is a recently-described regulator of cell shape changes in the developing nervous system. This protein is a member of a small family of related proteins that are defined by sequence similarity and in most cases by some link to the actin cytoskeleton. At present these proteins are named Shroom, APX, APXL, and KI...
journal_title:BMC cell biology
pub_type: 社论
doi:10.1186/1471-2121-7-18
更新日期:2006-04-14 00:00:00
abstract:BACKGROUND:Lipid droplets are a class of eukaryotic cell organelles for storage of neutral fat such as triacylglycerol (TAG) and cholesterol ester (CE). We and others have recently reported that lysosome-related organelles (LROs) are not fat storage structures in the nematode C. elegans. We also reported the formation ...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-11-96
更新日期:2010-12-08 00:00:00
abstract:BACKGROUND:Clathrin-mediated vesicular trafficking, the mechanism by which proteins and lipids are transported between membrane-bound organelles, accounts for a large proportion of import from the plasma membrane (endocytosis) and transport from the trans-Golgi network towards the endosomal system. Clathrin-mediated ev...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-15-23
更新日期:2014-06-19 00:00:00
abstract:BACKGROUND:Hedgehog (Hh) signaling is a conserved morphogenetic pathway which plays critical roles in embryonic development, with emerging evidence also supporting a role in healing and repair processes and tumorigenesis. The Gli family of transcription factors (Gli1, 2 and 3) mediate the Hedgehog morphogenetic signal ...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-9-49
更新日期:2008-09-13 00:00:00
abstract:BACKGROUND:Induced pluripotent stem cells (iPSC) are generated by reprogramming somatic cells into embryonic like state (ESC) using defined factors. There is great interest in these cells because of their potential for application in regenerative medicine. RESULTS:iPSC reprogrammed from murine tail tip fibroblasts wer...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-13-35
更新日期:2012-12-15 00:00:00
abstract:BACKGROUND:During cytokinesis, the cell's equator contracts against the cell's global stiffness. Identifying the biochemical basis for these mechanical parameters is essential for understanding how cells divide. To achieve this goal, the distribution and flux of the cell division machinery must be quantified. Here we r...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-3-4
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND:Endocytosis of activated EGF receptor (EGFR) to specific endocytic compartments is required to terminate EGF signaling. Trafficking of EGFR relies on microtubule tracks that transport the cargo vesicle to their intermediate and final destinations and can be modulated through posttranslational modification of...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/s12860-015-0070-8
更新日期:2015-09-18 00:00:00
abstract:BACKGROUND:The discovery of the importance of angiogenesis in tumor growth has emphasized the need to find specific vascular targets for tumor-targeted therapies. Previously, using phage display technology, we identified the peptide GX1 as having the ability to target the gastric cancer vasculature. The present study i...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-10-63
更新日期:2009-09-09 00:00:00
abstract:BACKGROUND:Recently, mast cells have been recognized to express several Toll-like receptors (TLRs) on their membrane surfaces, and granulocyte-macrophage colony-stimulating factor (GM-CSF) was reported to be able to alter expression of TLRs and cytokine production in neutrophils. However, whether GM-CSF modulates the e...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-10-37
更新日期:2009-05-09 00:00:00
abstract:BACKGROUND:Acrosomal proteins play crucial roles in the physiology of fertilization. Identification of proteins localizing to the acrosome is fundamental to the understanding of its contribution to fertilization. Novel proteins are still being reported from acrosome. In order to capture yet unreported proteins localizi...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-14-50
更新日期:2013-11-20 00:00:00
abstract:BACKGROUND:Mechanisms of long chain fatty acid uptake across the plasma membrane are important targets in treatment of many human diseases like obesity or hepatic steatosis. Long chain fatty acid translocation is achieved by a concert of co-existing mechanisms. These lipids can passively diffuse, but certain membrane p...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-9-45
更新日期:2008-08-13 00:00:00
abstract:BACKGROUND:It has been recognized that dermal fibroblasts and matrix metalloproteases (MMP) play crucial roles in wound healing process in skin. Thrombin was found to stimulate IL-8 release from human dermal fibroblasts (HDFs). However, little is known of the effect of thrombin on secretion of MMPs from dermal fibrobla...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-8-14
更新日期:2007-05-07 00:00:00
abstract:BACKGROUND:In this study, we have investigated the viscoelastic behaviour of individual human adult bone marrow-derived mesenchymal stem cells (hMSCs) and the role of F-actin filaments in maintaining these properties, using micropipette aspiration technique together with a standard linear viscoelastic solid model. RES...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-9-40
更新日期:2008-07-22 00:00:00
abstract:BACKGROUND:About 20 % of nemaline myopathies are thus far related to skeletal muscle alpha-actin. Seven actin mutants located in different parts of the actin molecule and linked to different forms of the disease were selected and expressed as EGFP-tagged constructs in differentiated C2C12 mytoubes. Results were compare...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-8-2
更新日期:2007-01-16 00:00:00
abstract:BACKGROUND:Ubiquitination serves multiple cellular functions, including proteasomal degradation and the control of stability, function, and intracellular localization of a wide variety of proteins. NEDD4L is a member of the HECT class of E3 ubiquitin ligases. A defining feature of NEDD4L protein isoforms is the presenc...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-10-26
更新日期:2009-04-13 00:00:00
abstract:BACKGROUND:Bone morphogenetic proteins (BMPs) contribute to many different aspects of development including mesoderm formation, heart development, neurogenesis, skeletal development, and axis formation. They have previously been recognized only as secreted growth factors, but the present study detected Bmp2, Bmp4, and ...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-11-20
更新日期:2010-03-15 00:00:00
abstract:BACKGROUND:There is a growing interest in cord blood as a source of primitive stem cells with the capacity for multilineage differentiation. Pure cell fractions are needed for the characterization and in vitro expansion of stem cells as well as for their use in preclinical research. However, enrichment of stem cells is...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-7-30
更新日期:2006-08-01 00:00:00
abstract:BACKGROUND:Adipose-Derived Stromal Cells have been shown to have multiple lineage differentiation properties and to be suitable for tissues regeneration in many degenerative processes. Their use has been proposed for the therapy of joint diseases and tendon injuries in the horse. In the present report the genetic manip...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-11-73
更新日期:2010-09-23 00:00:00
abstract:BACKGROUND:Spinal muscular atrophy is caused by reduced levels of the survival of motor neurons (SMN) protein. SMN is found in large complexes with Sm proteins and at least eight other proteins, including seven "gemins". These complexes are involved in the assembly of snRNPs in the cytoplasm and their transport into th...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-8-28
更新日期:2007-07-18 00:00:00
abstract:BACKGROUND:Fetal mesenchymal stem/stromal cells (MSC) represent a developmentally-advantageous cell type with translational potential.To enhance adult MSC migration, studies have focussed on the role of the chemokine receptor CXCR4 and its ligand SDF-1 (CXCL12), but more recent work implicates an intricate system of CX...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-15-15
更新日期:2014-05-16 00:00:00
abstract:BACKGROUND:In interferon-γ-stimulated cells, the dimeric transcription factor STAT1 (signal transducer and activator of transcription 1) recognizes semi-palindromic motifs in the promoter regions of cytokine-driven target genes termed GAS (gamma-activated sites). However, the molecular steps that facilitate GAS binding...
journal_title:BMC cell biology
pub_type: 杂志文章
doi:10.1186/1471-2121-13-22
更新日期:2012-08-24 00:00:00