Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells.

abstract：BACKGROUND:Published work suggests that some types of endothelial cells undergo apoptosis in response to ligation of the receptor Fas (CD95, APO1) but other types are resistant. Because heterogeneity among endothelial cells from different tissues, has been demonstrated, the purpose of this study was to determine, if Fa...

journal_title：BMC cell biology

authors： Filippatos G,Ang E,Gidea C,Dincer E,Wang R,Uhal BD

更新日期：2004-01-22 00:00:00

abstract：BACKGROUND:Cortical myosin-II filaments in Dictyostelium discoideum display enrichment in the posterior of the cell during cell migration, and in the cleavage furrow during cytokinesis. Filament assembly in turn is regulated by phosphorylation in the tail region of the myosin heavy chain (MHC). Early studies have revea...

journal_title：BMC cell biology

authors： Liang W,Licate L,Warrick H,Spudich J,Egelhoff T

更新日期：2002-07-24 00:00:00

abstract：BACKGROUND:In interferon-γ-stimulated cells, the dimeric transcription factor STAT1 (signal transducer and activator of transcription 1) recognizes semi-palindromic motifs in the promoter regions of cytokine-driven target genes termed GAS (gamma-activated sites). However, the molecular steps that facilitate GAS binding...

journal_title：BMC cell biology

authors： Koch V,Staab J,Ruppert V,Meyer T

更新日期：2012-08-24 00:00:00

abstract：BACKGROUND:Fetal mesenchymal stem/stromal cells (MSC) represent a developmentally-advantageous cell type with translational potential.To enhance adult MSC migration, studies have focussed on the role of the chemokine receptor CXCR4 and its ligand SDF-1 (CXCL12), but more recent work implicates an intricate system of CX...

journal_title：BMC cell biology

authors： Pelekanos RA,Ting MJ,Sardesai VS,Ryan JM,Lim YC,Chan JK,Fisk NM

更新日期：2014-05-16 00:00:00

abstract：BACKGROUND:There is a growing awareness of the importance of intracellular events in determining the outcome of infectious disease. To improve the understanding of such events, like phagosome maturation, we set out to develop a versatile technique for phagosome isolation that is rapid and widely applicable to different...

journal_title：BMC cell biology

authors： Lönnbro P,Nordenfelt P,Tapper H

更新日期：2008-06-27 00:00:00

abstract：BACKGROUND:Angiogenesis is the main therapeutic mechanism of cell therapy for cardiovascular diseases, but diabetes is reported to reduce the function and number of progenitor cells. Therefore, we studied the effect of streptozotocin-induced diabetes on the bone marrow-mesenchymal stem cell (MSC) function, and examined...

journal_title：BMC cell biology

authors： Kim YS,Kwon JS,Hong MH,Kang WS,Jeong HY,Kang HJ,Jeong Mh,Ahn Y

更新日期：2013-09-11 00:00:00

abstract：BACKGROUND:Chromatin insulators or boundary elements are a class of functional elements in the eukaryotic genome. They regulate gene transcription by interfering with promoter-enhancer communication. The Cp190 protein of Drosophila melanogaster is essential to the function of at least three-types of chromatin insulator...

journal_title：BMC cell biology

authors： Oliver D,Sheehan B,South H,Akbari O,Pai CY

更新日期：2010-12-31 00:00:00

abstract：BACKGROUND:Primary cilia are flagella-like projections from the centriole of mammalian cells that have a key role in cell signaling. Human diseases are linked to defects in primary cilia. Microtubules make up the axoneme of cilia and are selectively acetylated and this is thought to contribute to the stability of the s...

journal_title：BMC cell biology

authors： Wang W,Brautigan DL

更新日期：2008-11-26 00:00:00

abstract：BACKGROUND:The normal growth and function of mammary epithelial cells depend on interactions with the supportive stroma. Alterations in this communication can lead to the progression or expansion of malignant growth. The human mammary gland contains two distinctive types of fibroblasts within the stroma. The epithelial...

journal_title：BMC cell biology

authors： Fleming JM,Long EL,Ginsburg E,Gerscovich D,Meltzer PS,Vonderhaar BK

更新日期：2008-08-18 00:00:00

abstract：BACKGROUND:Clathrin-mediated vesicular trafficking, the mechanism by which proteins and lipids are transported between membrane-bound organelles, accounts for a large proportion of import from the plasma membrane (endocytosis) and transport from the trans-Golgi network towards the endosomal system. Clathrin-mediated ev...

journal_title：BMC cell biology

authors： Kalb LC,Frederico YC,Batista CM,Eger I,Fragoso SP,Soares MJ

更新日期：2014-06-19 00:00:00

abstract：BACKGROUND:The discovery of the importance of angiogenesis in tumor growth has emphasized the need to find specific vascular targets for tumor-targeted therapies. Previously, using phage display technology, we identified the peptide GX1 as having the ability to target the gastric cancer vasculature. The present study i...

journal_title：BMC cell biology

authors： Chen B,Cao S,Zhang Y,Wang X,Liu J,Hui X,Wan Y,Du W,Wang L,Wu K,Fan D

更新日期：2009-09-09 00:00:00

abstract：BACKGROUND:Ras proteins are guanine-nucleotide-binding enzymes that couple cell surface receptors to intracellular signaling pathways controlling cell proliferation and differentiation, both in lower and higher eukaryotes. They act as molecular switches by cycling between active GTP and inactive GDP-bound states, throu...

journal_title：BMC cell biology

authors： Arigoni M,Bracco E,Lusche DF,Kae H,Weeks G,Bozzaro S

更新日期：2005-12-07 00:00:00

abstract：BACKGROUND:Bone morphogenetic proteins (BMPs) and transforming growth factor-betas (TGF-betas) are important regulators of bone repair and regeneration. BMP-2 and TGF-beta1 have been shown to inhibit gap junctional intercellular communication (GJIC) in MC3T3-E1 cells. Connexin 43 (Cx43) has been shown to mediate GJIC i...

journal_title：BMC cell biology

authors： Wyatt LE,Chung CY,Carlsen B,Iida-Klein A,Rudkin GH,Ishida K,Yamaguchi DT,Miller TA

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:During cytokinesis, the cell's equator contracts against the cell's global stiffness. Identifying the biochemical basis for these mechanical parameters is essential for understanding how cells divide. To achieve this goal, the distribution and flux of the cell division machinery must be quantified. Here we r...

journal_title：BMC cell biology

authors： Robinson DN,Cavet G,Warrick HM,Spudich JA

更新日期：2002-01-01 00:00:00

abstract：BACKGROUND:The regulatory mechanisms of motor protein-dependent intracellular transport are still not fully understood. The kinesin-1-binding protein, JIP1, can function as an adaptor protein that links kinesin-1 and other JIP1-binding "cargo" proteins. However, it is unknown whether these "cargo" proteins influence th...

journal_title：BMC cell biology

authors： Satake T,Otsuki K,Banba Y,Suenaga J,Hirano H,Yamanaka Y,Ohno S,Hirai S

更新日期：2013-03-04 00:00:00

abstract：:Shroom is a recently-described regulator of cell shape changes in the developing nervous system. This protein is a member of a small family of related proteins that are defined by sequence similarity and in most cases by some link to the actin cytoskeleton. At present these proteins are named Shroom, APX, APXL, and KI...

journal_title：BMC cell biology

authors： Hagens O,Ballabio A,Kalscheuer V,Kraehenbuhl JP,Schiaffino MV,Smith P,Staub O,Hildebrand J,Wallingford JB

更新日期：2006-04-14 00:00:00

abstract：BACKGROUND:Mutations in the gene encoding for dysferlin cause recessive autosomal muscular dystrophies called dysferlinopathies. These mutations induce several alterations in skeletal muscles, including, inflammation, increased membrane permeability and cell death. Despite the fact that the etiology of dysferlinopathie...

journal_title：BMC cell biology

authors： Cea LA,Bevilacqua JA,Arriagada C,Cárdenas AM,Bigot A,Mouly V,Sáez JC,Caviedes P

更新日期：2016-05-24 00:00:00

abstract：BACKGROUND:Stimulation of Dictyostelium discoideum with cAMP evokes an elevation of the cytosolic free Ca2+ concentration ([Ca2+]i). The [Ca2+]i-change is composed of liberation of stored Ca2+ and extracellular Ca2+-entry. The significance of the [Ca2+]i-transient for chemotaxis is under debate. Abolition of chemotacti...

journal_title：BMC cell biology

authors： Schaloske RH,Lusche DF,Bezares-Roder K,Happle K,Malchow D,Schlatterer C

更新日期：2005-03-11 00:00:00

abstract：BACKGROUND:The mechanism involved in the maintenance and differentiation of embryonic stem (ES) cells is incompletely understood. RESULTS:To address this issue, we have developed a retroviral gene trap vector that can target genes expressed in undifferentiated ES cells. This gene trap vector harbors both GFP and Neo r...

journal_title：BMC cell biology

authors： Li X,Leder P

更新日期：2007-08-28 00:00:00

abstract：BACKGROUND:DNA hypermethylation is a key epigenetic mechanism for the silencing of many genes in cancer. Hinokitiol, a tropolone-related natural compound, is known to induce apoptosis and cell cycle arrest and has anti-inflammatory and anti-tumor activities. However, the relationship between hinokitiol and DNA methylat...

journal_title：BMC cell biology

authors： Seo JS,Choi YH,Moon JW,Kim HS,Park SH

更新日期：2017-02-27 00:00:00

abstract：BACKGROUND:Golgins are coiled-coil proteins associated with the Golgi apparatus, that are believed to be involved in the tethering of vesicles and the stacking of cisternae, as well as other functions such as cytoskeletal association. Many are peripheral membrane proteins recruited by GTPases. Several have been describ...

journal_title：BMC cell biology

authors： Fridmann-Sirkis Y,Siniossoglou S,Pelham HR

更新日期：2004-05-05 00:00:00

abstract：BACKGROUND:Spermatogenesis is comprised of a series of highly regulated developmental changes that transform the precursor germ cell into a highly specialized spermatozoon. The last phase of spermatogenesis, termed spermiogenesis, involves dramatic morphological change including formation of the acrosome, elongation an...

journal_title：BMC cell biology

authors： Wang R,Sperry AO

更新日期：2008-01-31 00:00:00

abstract：BACKGROUND:We have previously obtained a clonal population of cells from human foreskin that is able to differentiate into mesodermal, ectodermal and endodermal progenies. It is of great interest to know whether these cells could be further differentiated into functional insulin-producing cells. RESULTS:Sixty-one sing...

journal_title：BMC cell biology

authors： Bi D,Chen FG,Zhang WJ,Zhou GD,Cui L,Liu W,Cao Y

更新日期：2010-06-25 00:00:00

abstract：BACKGROUND:The transcription factor STAT3 (signal transducer and activator of transcription 3) is frequently activated in tumor cells. Activated STAT3 forms homodimers, or heterodimers with other TFs such as NF-κB, which becomes activated. Cytoplasmic STAT3 dimers are activated by tyrosine phosphorylation; they interac...

journal_title：BMC cell biology

authors： Souissi I,Najjar I,Ah-Koon L,Schischmanoff PO,Lesage D,Le Coquil S,Roger C,Dusanter-Fourt I,Varin-Blank N,Cao A,Metelev V,Baran-Marszak F,Fagard R

更新日期：2011-04-12 00:00:00

abstract：BACKGROUND:SPARC is a matricellular protein involved in cell-matrix interactions. From expression patterns at the wound site and in vitro studies, SPARC has been implicated in the control of wound healing. Here we examined the function of SPARC in cutaneous wound healing using SPARC-null mice and dermal fibroblasts der...

journal_title：BMC cell biology

authors： Basu A,Kligman LH,Samulewicz SJ,Howe CC

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:Hedgehog (Hh) signaling is a conserved morphogenetic pathway which plays critical roles in embryonic development, with emerging evidence also supporting a role in healing and repair processes and tumorigenesis. The Gli family of transcription factors (Gli1, 2 and 3) mediate the Hedgehog morphogenetic signal ...

journal_title：BMC cell biology

authors： Lipinski RJ,Bijlsma MF,Gipp JJ,Podhaizer DJ,Bushman W

更新日期：2008-09-13 00:00:00

abstract：BACKGROUND:Previously we cloned the human MNB/DYRK1A gene from the "Down syndrome critical region" on chromosome 21. This gene encodes a dual specificity protein kinase that catalyzes its autophosphorylation on serine/threonine and tyrosine residues. But, the functions of the MNB/DYRK1A gene in cellular processes are u...

journal_title：BMC cell biology

authors： Funakoshi E,Hori T,Haraguchi T,Hiraoka Y,Kudoh J,Shimizu N,Ito F

更新日期：2003-09-10 00:00:00

abstract：BACKGROUND:The MAL2 gene, encoding a four-transmembrane protein of the MAL family, is amplified and overexpressed in breast and other cancers, yet the significance of this is unknown. MAL-like proteins have trafficking functions, but their molecular roles are largely obscure, partly due to a lack of known binding partn...

journal_title：BMC cell biology

authors： Fanayan S,Shehata M,Agterof AP,McGuckin MA,Alonso MA,Byrne JA

更新日期：2009-01-28 00:00:00

abstract：BACKGROUND:Unc-45 is a myosin chaperone and a Hsp90 co-chaperone that plays a key role in muscle development. Genetic and biochemical studies in C. elegans have demonstrated that Unc-45 facilitates the process of myosin folding and assembly in body wall muscles. Loss or overexpression of Unc-45 in C. elegans results in...

journal_title：BMC cell biology

authors： Bernick EP,Zhang PJ,Du S

更新日期：2010-09-17 00:00:00

abstract：BACKGROUND:Lipid droplets are a class of eukaryotic cell organelles for storage of neutral fat such as triacylglycerol (TAG) and cholesterol ester (CE). We and others have recently reported that lysosome-related organelles (LROs) are not fat storage structures in the nematode C. elegans. We also reported the formation ...

journal_title：BMC cell biology

authors： Zhang SO,Trimble R,Guo F,Mak HY

更新日期：2010-12-08 00:00:00