Self-Condensation Culture Enables Vascularization of Tissue Fragments for Efficient Therapeutic Transplantation.


:Clinical transplantation of tissue fragments, including islets, faces a critical challenge because of a lack of effective strategies that ensure efficient engraftment through the timely integration of vascular networks. We recently developed a complex organoid engineering method by "self-condensation" culture based on mesenchymal cell-dependent contraction, thereby enabling dissociated heterotypic lineages including endothelial cells to self-organize in a spatiotemporal manner. Here, we report the successful adaptation of this method for generating complex tissues from diverse tissue fragments derived from various organs, including pancreatic islets. The self-condensation of human and mouse islets with endothelial cells not only promoted functionalization in culture but also massively improved post-transplant engraftment. Therapeutically, fulminant diabetic mice were more efficiently treated by a vascularized islet transplant compared with the conventional approach. Given the general limitations of post-transplant vascularization associated with 3D tissue-based therapy, our approach offers a promising means of enhancing efficacy in the context of therapeutic tissue transplantation.


Cell Rep


Cell reports


Takahashi Y,Sekine K,Kin T,Takebe T,Taniguchi H




Has Abstract


2018-05-08 00:00:00












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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Fong AP,Yao Z,Zhong JW,Johnson NM,Farr GH 3rd,Maves L,Tapscott SJ

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  • PlexinA2 Forward Signaling through Rap1 GTPases Regulates Dentate Gyrus Development and Schizophrenia-like Behaviors.

    abstract::Dentate gyrus (DG) development requires specification of granule cell (GC) progenitors in the hippocampal neuroepithelium, as well as their proliferation and migration into the primordial DG. We identify the Plexin family members Plxna2 and Plxna4 as important regulators of DG development. Distribution of immature GCs...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Zhao XF,Kohen R,Parent R,Duan Y,Fisher GL,Korn MJ,Ji L,Wan G,Jin J,Püschel AW,Dolan DF,Parent JM,Corfas G,Murphy GG,Giger RJ

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  • Synapse-to-Nucleus Communication through NFAT Is Mediated by L-type Ca2+ Channel Ca2+ Spike Propagation to the Soma.

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Wild AR,Sinnen BL,Dittmer PJ,Kennedy MJ,Sather WA,Dell'Acqua ML

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Chang GS,Chen XA,Park B,Rhee HS,Li P,Han KH,Mishra T,Chan-Salis KY,Li Y,Hardison RC,Wang Y,Pugh BF

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Salesse C,Charest J,Doucet-Beaupré H,Castonguay AM,Labrecque S,De Koninck P,Lévesque M

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Riggan L,Hildreth AD,Rolot M,Wong YY,Satyadi W,Sun R,Huerta C,O'Sullivan TE

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Avey D,Sankararaman S,Yim AKY,Barve R,Milbrandt J,Mitra RD

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  • The Apolipoprotein M/S1P Axis Controls Triglyceride Metabolism and Brown Fat Activity.

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Christoffersen C,Federspiel CK,Borup A,Christensen PM,Madsen AN,Heine M,Nielsen CH,Kjaer A,Holst B,Heeren J,Nielsen LB

    更新日期:2018-01-02 00:00:00

  • p53 Integrates Temporal WDR5 Inputs during Neuroectoderm and Mesoderm Differentiation of Mouse Embryonic Stem Cells.

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Li Q,Mao F,Zhou B,Huang Y,Zou Z,denDekker AD,Xu J,Hou S,Liu J,Dou Y,Rao RC

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Costa RO,Martins H,Martins LF,Cwetsch AW,Mele M,Pedro JR,Tomé D,Jeon NL,Cancedda L,Jaffrey SR,Almeida RD

    更新日期:2019-07-23 00:00:00

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Narayanan R,Pirouz M,Kerimoglu C,Pham L,Wagener RJ,Kiszka KA,Rosenbusch J,Seong RH,Kessel M,Fischer A,Stoykova A,Staiger JF,Tuoc T

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Qiu Z,Kala S,Guo J,Xian Q,Zhu J,Zhu T,Hou X,Wong KF,Yang M,Wang H,Sun L

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Edison N,Curtz Y,Paland N,Mamriev D,Chorubczyk N,Haviv-Reingewertz T,Kfir N,Morgenstern D,Kupervaser M,Kagan J,Kim HT,Larisch S

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Garay PM,Chen A,Tsukahara T,Rodríguez Díaz JC,Kohen R,Althaus JC,Wallner MA,Giger RJ,Jones KS,Sutton MA,Iwase S

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Veland N,Hardikar S,Zhong Y,Gayatri S,Dan J,Strahl BD,Rothbart SB,Bedford MT,Chen T

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Tambe A,East-Seletsky A,Knott GJ,Doudna JA,O'Connell MR

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Zhang Y,Yang L,Kucherlapati M,Chen F,Hadjipanayis A,Pantazi A,Bristow CA,Lee EA,Mahadeshwar HS,Tang J,Zhang J,Seth S,Lee S,Ren X,Song X,Sun H,Seidman J,Luquette LJ,Xi R,Chin L,Protopopov A,Li W,Park PJ,Kuche

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Masters TA,Tumbarello DA,Chibalina MV,Buss F

    更新日期:2017-06-06 00:00:00

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Louros SR,Hooks BM,Litvina L,Carvalho AL,Chen C

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Zhang Q,Zhang Y,Wang C,Xu Z,Liang Q,An L,Li J,Liu Z,You Y,He M,Mao Y,Chen B,Xiong ZQ,Rubenstein JL,Yang Z

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Meyers CA,Lee S,Sono T,Xu J,Negri S,Tian Y,Wang Y,Li Z,Miller S,Chang L,Gao Y,Minichiello L,Clemens TL,James AW

    更新日期:2020-05-26 00:00:00

  • Tumor-Expressed IDO Recruits and Activates MDSCs in a Treg-Dependent Manner.

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Holmgaard RB,Zamarin D,Li Y,Gasmi B,Munn DH,Allison JP,Merghoub T,Wolchok JD

    更新日期:2015-10-13 00:00:00

  • The TIP60 Complex Is a Conserved Coactivator of HIF1A.

    abstract::Hypoxia-inducible factors (HIFs) are critical regulators of the cellular response to hypoxia. Despite their established roles in normal physiology and numerous pathologies, the molecular mechanisms by which they control gene expression remain poorly understood. We report here a conserved role for the TIP60 complex as ...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Perez-Perri JI,Dengler VL,Audetat KA,Pandey A,Bonner EA,Urh M,Mendez J,Daniels DL,Wappner P,Galbraith MD,Espinosa JM

    更新日期:2016-06-28 00:00:00

  • Nutrient-Dependent Trade-Offs between Ribosomes and Division Protein Synthesis Control Bacterial Cell Size and Growth.

    abstract::Cell size control emerges from a regulated balance between the rates of cell growth and division. In bacteria, simple quantitative laws connect cellular growth rate to ribosome abundance. However, it remains poorly understood how translation regulates bacterial cell size and shape under growth perturbations. Here, we ...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Serbanescu D,Ojkic N,Banerjee S

    更新日期:2020-09-22 00:00:00

  • CD57+ Memory T Cells Proliferate In Vivo.

    abstract::A central paradigm in the field of lymphocyte biology asserts that replicatively senescent memory T cells express the carbohydrate epitope CD57. These cells nonetheless accumulate with age and expand numerically in response to persistent antigenic stimulation. Here, we use in vivo deuterium labeling and ex vivo analys...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Ahmed R,Miners KL,Lahoz-Beneytez J,Jones RE,Roger L,Baboonian C,Zhang Y,Wang ECY,Hellerstein MK,McCune JM,Baird DM,Price DA,Macallan DC,Asquith B,Ladell K

    更新日期:2020-12-15 00:00:00

  • Structural Insights into the Unique Activation Mechanisms of a Non-classical Calpain and Its Disease-Causing Variants.

    abstract::Increased calpain activity is linked to neuroinflammation including a heritable retinal disease caused by hyper-activating mutations in the calcium-activated calpain-5 (CAPN5) protease. Although structures for classical calpains are known, the structure of CAPN5, a non-classical calpain, remains undetermined. Here we ...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Velez G,Sun YJ,Khan S,Yang J,Herrmann J,Chemudupati T,MacLaren RE,Gakhar L,Wakatsuki S,Bassuk AG,Mahajan VB

    更新日期:2020-01-21 00:00:00

  • Accurate Drug Repositioning through Non-tissue-Specific Core Signatures from Cancer Transcriptomes.

    abstract::Experimental large-scale screens for drug repositioning are limited by restriction to in vitro conditions and lack of applicability to real human conditions. Here, we developed an in silico screen in human in vivo conditions using a reference of single gene mutations' non-tissue-specific "core transcriptome signatures...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Xu C,Ai D,Shi D,Suo S,Chen X,Yan Y,Cao Y,Zhang R,Sun N,Chen W,McDermott J,Zhang S,Zeng Y,Han JJ

    更新日期:2018-10-09 00:00:00

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Rydén M,Hrydziuszko O,Mileti E,Raman A,Bornholdt J,Boyd M,Toft E,Qvist V,Näslund E,Thorell A,Andersson DP,Dahlman I,Gao H,Sandelin A,Daub CO,Arner P

    更新日期:2016-08-30 00:00:00

  • The Spindle Assembly Checkpoint Is Not Essential for Viability of Human Cells with Genetically Lowered APC/C Activity.

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Wild T,Larsen MS,Narita T,Schou J,Nilsson J,Choudhary C

    更新日期:2016-03-01 00:00:00

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    abstract::Stable wakefulness requires orexin/hypocretin neurons (OHNs) and OHR2 receptors. OHNs sense diverse environmental cues and control arousal accordingly. For unknown reasons, OHNs contain multiple excitatory transmitters, including OH peptides and glutamate. To analyze their cotransmission within computational framework...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Schöne C,Apergis-Schoute J,Sakurai T,Adamantidis A,Burdakov D

    更新日期:2014-05-08 00:00:00