Abstract：
:In budding yeast, a mother cell can produce a finite number of daughter cells before it stops dividing and dies. Such entry into senescence is thought to result from a progressive decline in physiological function, including a loss of mitochondrial membrane potential (ΔΨ). Here, we developed a microfluidic device to monitor the dynamics of cell division and ΔΨ in real time at single-cell resolution. We show that cells do not enter senescence gradually but rather undergo an abrupt transition to a slowly dividing state. Moreover, we demonstrate that the decline in ΔΨ, which is observed only in a fraction of cells, is not responsible for entry into senescence. Rather, the loss of ΔΨ is an age-independent and heritable process that leads to clonal senescence and is therefore incompatible with daughter cell rejuvenation. These results emphasize the importance of quantitative single-cell measurements to decipher the causes of cellular aging.
journal_name
Cell Repjournal_title
Cell reportsauthors
Fehrmann S,Paoletti C,Goulev Y,Ungureanu A,Aguilaniu H,Charvin Gdoi
10.1016/j.celrep.2013.11.013subject
Has Abstractpub_date
2013-12-26 00:00:00pages
1589-99issue
6issn
2211-1247pii
S2211-1247(13)00680-3journal_volume
5pub_type
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