Abstract:
:The preadipocyte differentiation biological process involves a cascade of transcriptional events that culminates in the expression of peroxisome proliferator-activated receptor (PPAR) γ. The differentiation cocktail [insulin (INS), dexamethasone (DEX) and isobutylmethylxanthine (IBMX)] can induce preadipocyte differentiation in mammals, but it is insufficient for chicken (Gallus gallus) adipogenesis. Oleate can induce chicken preadipocyte differentiation, but these differentiated preadipocytes may not be fully functional. The objective of the current study was to evaluate whether chicken preadipocytes can be induced to mature adipocytes by a novel induction method using differentiation cocktail supplemented with PPARγ agonist(s). Chicken preadipocytes cultured in cocktail supplemented with rosiglitazone or troglitazone resulted in a marked increase in lipid droplet accumulation (P<0.05), glycerol-3-phosphate dehydrogenase (GPDH) activity (P<0.05), mRNA expression level of adipocyte fatty acid-binding protein (aP2; P<0.05), G0/G1 switch gene 2 (G0S2; P<0.05) and lipolysis (P<0.05). In addition, supplementation of the cocktail with rosiglitazone promoted PPARγ mRNA expression (P<0.05). In conclusion, our data indicated that chicken preadipocytes can be induced to mature adipocytes using differentiation cocktail supplemented with rosiglitazone. The results of the present study provide a novel induction method for in vitro chicken preadipocyte differentiation.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Cheng B,Wu M,Xu S,Zhang X,Wang Y,Wang N,Leng L,Li Hdoi
10.1042/BSR20160049subject
Has Abstractpub_date
2016-11-03 00:00:00issue
6eissn
0144-8463issn
1573-4935pii
BSR20160049journal_volume
36pub_type
杂志文章abstract::Carnosic acid (CA), a major polyphenolic diterpene present in Rosmarinus officinalis, has been reported to have multiple functions, including antitumor activity. The MTT assay, BrdU incorporation, wound healing, and colony formation were used to detect melanoma B16F10 cell growth and proliferation. Flow cytometry was ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180005
更新日期:2018-07-02 00:00:00
abstract::The principle of the dual inhibitor titration method for testing models of electron-transport phosphorylation is outlined, and the method is applied to the study of photophosphorylation in bacterial chromatophores. It is concluded that energy coupling is strictly localized in nature in this system, in the sense that f...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114933
更新日期:1982-10-01 00:00:00
abstract::Background: Many epidemiologic studies have explored the association between age at last birth (ALB) and the risk of epithelial ovarian cancer, but the results remain controversial. Methods: A literature search was performed in PubMed, Web of Science, China National Knowledge Infrastructure (CNKI) and WanFang Med Onli...
journal_title:Bioscience reports
pub_type: 杂志文章,meta分析
doi:10.1042/BSR20182035
更新日期:2019-09-13 00:00:00
abstract::In rats fed a normal (22% protein) diet, injection of clenbuterol (1 mg/kg/d for 21 d) did not affect energy intake, energy expenditure or weight gain, but reduced energetic efficiency, and fat and energy gains and increased body protein content. Presenting a low-protein (8%) diet reduced energy intake, gain and effic...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122126
更新日期:1987-12-01 00:00:00
abstract::Poly(A)+RNA was isolated from brown adipose tissue of cold acclimated rats and a fraction enriched for uncoupling protein mRNA was used to generate a cDNA library in pBR 322. Immunological screening of 1,500 colonies with an affinity-purified antiserum against the uncoupling protein yielded five positive clones, pUCPr...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01145183
更新日期:1986-01-01 00:00:00
abstract::The human bronchial epithelial cell line, 16HBE14o- (16HBE), is widely used as a model for respiratory epithelial diseases and barrier function. During differentiation, transepithelial electrical resistance (TER) increased to approximately 800 Ohms × cm2, while 14C-d-mannitol flux rates (Jm) simultaneously decreased. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20201532
更新日期:2020-10-30 00:00:00
abstract::Perfusion of Langendorff rat hearts with [14C]adenosine yields an acid-insoluble, radioactive product whose concentration falls during ischaemia. The properties of the substance show that it is a polyribonucleotide. It is suggested that it may be mitochondrial poly A acting as a storage form of adenine nucleotides. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117435
更新日期:1985-01-01 00:00:00
abstract::A consensus about the prognostic role of NIMA-related kinase 2 (NEK2) expression in various solid tumors has not been made yet. Thus, this meta-analysis aimed to systematically assess the prognostic role of NEK2 expression in patients with solid tumors. The eligible studies were identified through searching PubMed, We...
journal_title:Bioscience reports
pub_type: 杂志文章,meta分析
doi:10.1042/BSR20180618
更新日期:2019-01-18 00:00:00
abstract::Mitochondrial dysfunction causes a range of early-onset neurological diseases and contributes to neurodegenerative conditions. The mechanisms of neurological damage however are poorly understood, as accessing relevant tissue from patients is difficult, and appropriate models are limited. Hence, we assessed mitochondri...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140151
更新日期:2014-11-21 00:00:00
abstract::The present study investigated the effects of Isorhamnetin on two types of prostate cancer cells (androgen-independent and androgen-dependent) and explored its possible mechanisms underlying such effects. Treatment with Isorhamnetin significantly inhibited cell growth and induced lactate dehydrogenase (LDH) release of...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20192826
更新日期:2020-03-27 00:00:00
abstract::Prostate cancer (PCa), the second most mortal cancer from developed countries, presents a high level of chemoresistance. There is emerging evidence underscores the critical role of autophagy in the onset, progression, and chemoresistance of PCa. In the present study, we investigated the possible role of a novel autoph...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170770
更新日期:2019-08-23 00:00:00
abstract::Osteoarthritis (OA) is the most common form of arthritis, and connective tissue growth factor (CTGF) is found to be up-regulated in adjacent areas of cartilage surface damage. CTGF is present in osteophytes of late stage OA. In the present study, we have reviewed association of CTGF in the development and progression ...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1042/BSR20191374
更新日期:2019-07-18 00:00:00
abstract::Resistance to chemotherapy is one of main obstacles in the treatment of colorectal cancer (CRC). However, the mechanisms are still unclear, and the treatment options are still limited. miR-543 has been indicated to act as an oncogene in some cancers, but its function in regulating chemoresistance has not been consider...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190249
更新日期:2019-03-22 00:00:00
abstract::Extracts from uterine cervical and body cancers, but not from benign tumor or intact tissues tested, were found to contain a growth-promoting activity which induced the proliferation of human endometrial fibroblasts. Exposure of cultured fibroblasts to the cancer extracts increased the rate of [3H]thymidine incorporat...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116850
更新日期:1990-02-01 00:00:00
abstract::Although radioactive iodine (131I) treatment (RIT) is recommended as the preferred option for patients with Graves' disease (GD), the predictive factors for its clinical outcomes are still unclear. In the present study, we aim to investigate the factors influencing the success rate of RIT treatment on primary GD with ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20191609
更新日期:2020-01-31 00:00:00
abstract::The tetracycline repressor (TetR)-regulated system is a widely used tool to study gene functions through control of its expression. Various effectors such as tetracycline (Tc) and doxycycline (Dox) quickly induce or shut down gene expression, but reversing gene expression has not been eligible due to long half-lives o...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180793
更新日期:2019-01-08 00:00:00
abstract::Goblet cells (GCs) are single-cell glands that produce and secrete mucin. Mucin forms a mucus layer, which can separate the materials in cavities from the intestinal epithelium and prevent the invasion of pathogenic microorganisms in various ways. GCs can also participate in the immune response through nonspecific end...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20201471
更新日期:2020-10-30 00:00:00
abstract::A model explaining quantal Ca2+ release as an intrinsic property of the inositol 1,4,5-triphosphate (IP3) receptor has been put forward. The model is based on the hypothesis that the IP3 receptor can catalyze a transformation of the IP3 molecule differing from its conventional metabolism. A simple kinetic mechanism is...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01207265
更新日期:1996-10-01 00:00:00
abstract::Mouse Mex3c encodes RNA-binding proteins of variant length through alternative splicing. Its mutation results in multiple defects including growth retardation, perturbed energy balance, and defective antiviral innate immunity. Here we report that Mex3c mutation affects mammary gland development and lactation in female...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20201285
更新日期:2020-12-23 00:00:00
abstract::Rat ventral prostate incorporated (1-14C)acetate, (1-14C)palmitate and (1-14C)linoleate into different phospholipids in a time-dependent process. The rate of incorporation into total phospholipids was higher with linoleate (10.0 nmol/g) than with either palmitate (5.8 nmol/g) or acetate (4.7 nmol/g). Predominant label...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116858
更新日期:1990-02-01 00:00:00
abstract::Erythrocyte membrane is crucial to maintain the stability of erythrocyte structure. The membrane protein on the surface of erythrocyte membrane enables erythrocyte to have plasticity and pass through the microcirculation without being blocked or destroyed. Decreased deformability of erythrocyte membrane protein will l...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200799
更新日期:2020-06-26 00:00:00
abstract::High concentrations of glucose and palmitate increase endothelial cell inflammation and apoptosis, events that often precede atherogenesis. They may do so by decreasing basal autophagy and AMP-activated protein kinase (AMPK) activity, although the mechanisms by which this occurs are not clear. Decreased function of th...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160174
更新日期:2016-09-16 00:00:00
abstract::LncRNA PACER is a chondrocyte inflammation-associated long non-coding RNA (lncRNA), and chondrocyte inflammation is involved in osteoarthritis (OA). We observed that plasma PACER was down-regulated, while plasma HOTAIR was up-regulated in OA patients. Altered plasma levels of PACER and HOTAIR distinguished OA patients...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190404
更新日期:2019-06-07 00:00:00
abstract::A novel approach was used to assess the role of phosphoinositide hydrolysis in the mitogenic action of phytohemagglutinin (PHA) or concanavalin A (ConA). The treatment of human peripheral blood leukocytes (PBL) with monospecific antibodies against phospholipase C (PLC) produced a dose-dependent inhibition (up to 100%)...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116866
更新日期:1987-09-01 00:00:00
abstract::Retinitis pigmentosa (RP) is a group of hereditary, degenerative retinal disorders characterized by progressive retinal dysfunction, outer retina cell loss, and retinal tissue atrophy. It eventually leads to tunnel vision and legal, or total blindness. Here we aimed to reveal the causal gene and mutation contributing ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20171300
更新日期:2018-04-27 00:00:00
abstract::When the amino-acid sequence of the 108-residue, rat tumour calcium-binding protein, oncomodulin, was aligned with that of rat muscle parvalbumin, 55 homologous positions were found, with an additional 33 single base-pair substitutions. This extensive homology, with virtual identity of the calcium-binding domains, sig...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121034
更新日期:1983-11-01 00:00:00
abstract::To observe the effect of gene expression and tumorigenicity in hybrid cells of human embryonic stem cells (hESCs) and ovarian cancer cells in vitro and in vivo using a mouse model, and to determine its feasibility in reprogramming tumour cells growth and apoptosis, for a potential exploration of the role of hESCs and ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160104
更新日期:2016-09-16 00:00:00
abstract::Erectile dysfunction (ED) is one of the significant complications of diabetes mellitus (DM), and CASR plays an important role in cellular antiapoptosis and NO production in the vascular endothelium by activating PKC. The present study was aimed to investigate the efficacy of Leech and Centipede Granules (LCG) through ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20193845
更新日期:2020-02-28 00:00:00
abstract::Matrix-bound monoclonal antibodies against urokinase have been used to purify this enzyme by affinity chromatography. In a single-step procedure, urokinase can be isolated from crude preparations with high yield and high purity, and without loss of enzymatic activity. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122902
更新日期:1983-04-01 00:00:00
abstract::The proton conductance of the mitochondrial inner membrane can be quantified by applying Ohm's law to the experimentally determined protonmotive force and the proton current flowing around the proton circuit in the absence of ATP synthesis or ion transport. This last parameter is derived from the rate of State 4 respi...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1023/a:1027376426860
更新日期:1997-06-01 00:00:00