Abstract:
:A novel approach was used to assess the role of phosphoinositide hydrolysis in the mitogenic action of phytohemagglutinin (PHA) or concanavalin A (ConA). The treatment of human peripheral blood leukocytes (PBL) with monospecific antibodies against phospholipase C (PLC) produced a dose-dependent inhibition (up to 100%) of PHA (10 micrograms/ml) or ConA (25 micrograms/ml) proliferative effects. Thus, the activation of membrane-bound PLC is a sine-qua-non condition for lectin-induced proliferation of T lymphocytes. The key-role of PLC versus protein kinase C (PKC) is stressed by the fact that the inhibition of PKC with Hidaka's compound H-7 (40 microM) produced only a partial blockade (about 25%) of lectin mitogenic effect.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Moraru II,Manciulea M,Călugăru A,Ghyka G,Popescu LMdoi
10.1007/BF01116866subject
Has Abstractpub_date
1987-09-01 00:00:00pages
731-6issue
9eissn
0144-8463issn
1573-4935journal_volume
7pub_type
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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更新日期:1995-10-01 00:00:00
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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