Abstract:
:NUF2 (NUF2, Ndc80 kinetochore complex component) plays an important role in kinetochore-microtubule attachment. It has been reported that NUF2 is associated with multiple human cancers. However, the functional role of NUF2 in pancreatic cancer remains unclear. In this study, we found that NUF2 expression was stronger in tumour tissues than in normal pancreatic tissues, and its overexpression could be related to poor prognosis. Moreover, NUF2 was highly expressed in several human pancreatic cancer cell lines. We took advantage of lentivirus-mediated siRNA (small interfering RNA) to suppress NUF2 expression in PANC-1 and Sw1990 cell lines aiming to investigate the role of NUF2 in pancreatic cancer. NUF2 silencing by RANi (RNA interference) reduced the proliferation and colony formation ability of pancreatic cancer cells in vitro. Cell cycle analysis showed that NUF2 knockdown induced cell cycle arrest at G0/G1 phase via suppression of Cyclin B1, Cdc2 and Cdc25A. More importantly, NUF2 silencing was able to alleviate in vivo tumourigenesis in pancreatic cancer xenograft nude mice. Collectively, the present study indicates that the siRNA-mediated knockdown against NUF2 may be a promising therapeutic method for the treatment of pancreatic cancer.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Hu P,Chen X,Sun J,Bie P,Zhang LDdoi
10.1042/BSR20140124subject
Has Abstractpub_date
2015-01-14 00:00:00issue
1eissn
0144-8463issn
1573-4935pii
BSR20140124journal_volume
35pub_type
杂志文章abstract::In recent decades, many genome-wide association studies on insomnia have reported numerous genes harboring multiple risk variants. Nevertheless, the molecular functions of these risk variants conveying risk to insomnia are still ill-studied. In the present study, we integrated GWAS summary statistics (N=386,533) with ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20201084
更新日期:2020-09-30 00:00:00
abstract::Background: As one of the most common liver disorders worldwide, non-alcoholic fatty liver disease (NAFLD) begins with the abnormal accumulation of triglyceride (TG) in the liver. Long non-coding RNA-H19 was reported to modulate hepatic metabolic homeostasis in NAFLD. However, its molecular mechanism of NAFLD was not ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181722
更新日期:2019-07-15 00:00:00
abstract::The effect of ligands (glucose, ATP and Mg2+) and zwitterionic micelles of lysophosphatidylcholine (LPC) or N-hexadecyl-N,N-dimethyl-3-ammonium propanesulfonate (HPS) in the yeast hexokinase (HK) stability was studied at 35 degrees C. The thermal inactivation kinetics followed one-exponential decay. The effect of liga...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1023/a:1005583117296
更新日期:2000-02-01 00:00:00
abstract::Calmodulin (CaM)-like activity was detected in human serum and foetal calf serum, with an activity 10 times more than that detectable in plasma. Serum CaM was largely accounted for by release from human platelets as confirmed by both radioimmunoassay and sodium-dodecyl-sulphate/polyacrylamide-gel electrophoresis of Ca...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121017
更新日期:1984-08-01 00:00:00
abstract::Long noncoding RNA (lncRNA) differentiation antagonizing nonprotein coding RNA (DANCR) plays important regulatory roles in many solid tumors. However, the effect of DANCR in glioma progression and underlying molecular mechanisms were not entirely explored. In the present study, we determined the expression of DANCR in...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20171664
更新日期:2018-02-02 00:00:00
abstract::The concentration of Na+ and K+ and the pH in the cytoplasm of Lettré cells was measured by monitoring the net flux of H+, Na+, or K+ across the plasma membrane which had been rendered permeable to these ions by the action of Sendai virus. Ion flux was measured directly by analysis of cell composition, or indirectly b...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01172873
更新日期:1983-07-01 00:00:00
abstract::The growth arrest special 5 (GAS5), as a research hotspot of long noncoding RNAs (lncRNAs), has been reported to be associated with colorectal cancer (CRC). However, the association between polymorphisms in GAS5 and the risk of CRC was not clear. In the present study, a case-control study in 1078 CRC patients and 1175...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190091
更新日期:2019-04-16 00:00:00
abstract::MicroRNA-326 (miR-326) was reported to be dysregulated and involved in the progression of multiple cancers. However, the clinical significance, biological role and underlying mechanism of miR-326 in the carcinogenesis of breast cancer are still unclear. In the present study, we showed that miR-326 was down-regulated i...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190787
更新日期:2019-07-29 00:00:00
abstract::Kawasaki disease (KD) is a type of disease that includes the development of a fever that lasts at least 5 days and involves the clinical manifestation of multicellular vasculitis. KD has become one of the most common pediatric cardiovascular diseases. Previous studies have reported that miR-218 rs11134527 A>G is assoc...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180367
更新日期:2018-05-22 00:00:00
abstract:OBJECTIVE:The present study aimed to construct a diagnosis model for the early differentiation of acute radiation pneumonitis (ARP) and infectious pneumonitis based on multiple parameters. METHODS:The present study included data of 152 patients admitted to the Department of Radiochemotherapy, Tangshan People's Hospita...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200299
更新日期:2020-04-30 00:00:00
abstract::Alterations in genomic CpG methylation patterns have been found to be associated with cell transformation and neoplasia. Although it is recognized that methylation of CpG residues negatively regulates gene expression, how the various MBPs (methyl-binding proteins) contribute to this process remains elusive. To determi...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20080032
更新日期:2008-12-01 00:00:00
abstract::Native bovine parathyroid hormone (bPTH) was found to be readily cleaved with human leukocyte elastase to yield the fragments bPTH(1-41) and bPTH(42-84). These were then isolated by reverse-phase HPLC and characterised by gas-phase sequencing and amino acid analysis. The biological activities of these fragments were a...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122726
更新日期:1987-01-01 00:00:00
abstract::Calpain-1 and calpain-2 are highly structurally similar isoforms of calpain. The calpains, a family of intracellular cysteine proteases, cleave their substrates at specific sites, thus modifying their properties such as function or activity. These isoforms have long been considered to function in a redundant or comple...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200552
更新日期:2020-11-27 00:00:00
abstract::An antibody was raised to the synthetic pentapeptide pGluHisProGlyLys which, in radioimmunoassay (RIA), could detect the pentapeptide at a level of 10 fmole per tube and exhibited less than 0.5 per cent cross reactivity with a series of related peptides. The RIA was used to demonstrate the presence of C-terminally ext...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114948
更新日期:1986-06-01 00:00:00
abstract::LncRNA PACER is a chondrocyte inflammation-associated long non-coding RNA (lncRNA), and chondrocyte inflammation is involved in osteoarthritis (OA). We observed that plasma PACER was down-regulated, while plasma HOTAIR was up-regulated in OA patients. Altered plasma levels of PACER and HOTAIR distinguished OA patients...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190404
更新日期:2019-06-07 00:00:00
abstract::A major cause of failure in chemotherapy is drug resistance of cancer cells. Exosomes have been introduced to spread chemoresistance through delivering miRNAs. However, a systematic evaluation of the exosomal miRNA expression profiles responsible for chemoresistance is still lacking. In the present study, miRNA signat...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181090
更新日期:2018-11-15 00:00:00
abstract:OBJECTIVE:Gastric cancer is the most common gastrointestinal malignancy in China and results from a combination of genetic and environmental factors. The present study was conducted to investigate the relationship between long noncoding RNA (lncRNA) materally expressed gene 3 (MEG3) single nucleotide polymorphisms (SNP...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200305
更新日期:2020-11-27 00:00:00
abstract::Myelin Basic Protein, one of the major membrane protein component of the central nervous system, was used to probe the molecular mechanism of cellular activation by phytohaemagglutinin. Pre-treatment of human lymphocytes with myelin basic protein results in a lower rising of cytosolic concentration of free calcium aft...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116851
更新日期:1990-02-01 00:00:00
abstract::One of the major obstacles to obtaining human cells of a defined and reproducible standard suitable for use as medical therapies is the necessity for FCS (fetal calf serum) media augmentation in routine cell culture applications. FCS has become the supplement of choice for cell culture research, as it contains an arra...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20100064
更新日期:2011-06-01 00:00:00
abstract::Muscle tissue levels of d1-alpha-tocopherol (vitamin E) were significantly lower in endurance-trained rats than in sedentary animals, whether the animals were fed on vitamin-E-deficient or control (vitamin-E-sufficient) diets. In vitamin-E-deficient rats, liver tissue levels of vitamin E were significantly lower in th...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119661
更新日期:1984-03-01 00:00:00
abstract::The ways in which certain inorganic elements are used in essential roles within biological systems generally are described. The two roles stressed are (1) electrolytic charge movements and (2) electronic charge movement. The value of helical proteins inside and outside membranes as mechanical coupling devices is analy...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117344
更新日期:1988-12-01 00:00:00
abstract:BACKGROUND:Polycystic ovarian syndrome (PCOS) is a kind of common gynecological endocrine disorder. And the mutations of melatonin receptor (MTNR) genes are related to the occurrence of PCOS. But previous researches have shown opposite results. So, the object of our systematic review and meta-analysis is to investigate...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200824
更新日期:2020-06-26 00:00:00
abstract::miRNAs are non-coding RNA molecules typically 18-22 nucleotides long that can suppress the expression of their target genes. Several laboratories have attempted to identify miRNAs from the pig that are involved in Salmonella infection. These bioinformatics strategies using the newly available genomic sequence are gene...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160243
更新日期:2016-08-31 00:00:00
abstract::Thrombospondins (TSPs) are evolutionarily-conserved, secreted glycoproteins that interact with cell surfaces and extracellular matrix (ECM) and have complex roles in cell interactions. Unlike the structural components of the ECM that form networks or fibrils, TSPs are deposited into ECM as arrays of nanoscale puncta. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140168
更新日期:2015-05-20 00:00:00
abstract::Having a better grasp of the molecular mechanisms underlying carcinogenesis and progression in osteosarcoma would be helpful to find novel therapeutic targets. Different types of cancers have presented abnormal expression of miRNA-101 (miR-101). Nevertheless, we still could not figure out what expression of miR-101 in...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181283
更新日期:2019-02-08 00:00:00
abstract::Using both immunohistological study and photoaffinity labelling with radioactive azido-ATP, evidence is presented that the mitochondrial membranes of the brown adipose tissue of the human adult contain a 32 000-Mr uncoupling protein, which is probably similar to the uncoupling protein of BAT of rodents. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01120989
更新日期:1983-08-01 00:00:00
abstract::An adenosine 3'5'-cyclic-monophosphate (Cyclic AMP)-dependent protein kinase has been identified and partially purified from the rat prostate tumor induced by 20-methylcholanthrene. This enzyme is stimulated 2- to 3-fold by the nucleotide. Equilibrium studies at pH 5.0 suggest the presence of a major class of binding ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114829
更新日期:1982-09-01 00:00:00
abstract::The concentration of carbonic anhydrase III (CAIII) in male rat liver was found to be 30 times greater than that in the female. Castration of male rats led to marked reduction in liver CAIII concentrations which could be partially restored to control levels by testosterone replacement. Marked developmental and senesce...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121959
更新日期:1983-05-01 00:00:00
abstract::The present study was conducted with the aim being to investigate the effect γ-aminobutyric acid type B (GABAB) receptor activation have on spatial cognitive function and hippocampal neurones found in the rat models of type 2 diabetes mellitus (T2DM). T2DM rat models were then established, randomized, and subsequently...
journal_title:Bioscience reports
pub_type: 杂志文章,收录出版
doi:10.1042/BSR20171184
更新日期:2018-01-19 00:00:00
abstract:BACKGROUND:This research aimed to study the associations between XPD (G751A, rs13181), hOGG1 (C326G, rs1052133) and XRCC4 (G1394T, rs6869366) gene polymorphisms and the risk of colorectal cancer (CRC) in a Chinese Han population. METHOD:A total of 225 Chinese Han patients with CRC were selected as the study group, and...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181074
更新日期:2019-01-15 00:00:00