A novel method for measuring intracellular pH and potassium concentration.

abstract：:Alterations in genomic CpG methylation patterns have been found to be associated with cell transformation and neoplasia. Although it is recognized that methylation of CpG residues negatively regulates gene expression, how the various MBPs (methyl-binding proteins) contribute to this process remains elusive. To determi...

journal_title：Bioscience reports

authors： Yaqinuddin A,Abbas F,Naqvi SZ,Bashir MU,Qazi R,Qureshi SA

更新日期：2008-12-01 00:00:00

abstract：:Emerging evidence are accumulating that long noncoding RNAs (lncRNAs) have recently been identified to participate in various cellular processes. Terminal differentiation induced ncRNA (TINCR) is a newly identified lncRNA with its functional roles not fully elucidated in human malignancy. The current study aims to ide...

journal_title：Bioscience reports

authors： Tian F,Xu J,Xue F,Guan E,Xu X

更新日期：2017-07-27 00:00:00

abstract：:Doxorubicin (DOX) resistance in breast cancer largely results from the breast cancer stem cell like cells (BCSCs) which could be targetted to improve the efficacy of chemotherapy. Cell permeabilization using microbubbles (MBs) and ultrasound (US) have the potential for delivering molecules into the cytoplasm. We aim t...

journal_title：Bioscience reports

authors： Guo L,Zheng P,Fan H,Wang H,Xu W,Zhou W

更新日期：2017-11-09 00:00:00

abstract：:Hepatocellular carcinoma (HCC) has a high recurrence rate and poor clinical outcome after currently used therapies, including radiofrequency ablation. To explore the possible mechanisms for the relapse of HCC, in the present study we focussed on long non-coding RNA (LncRNA), which has been reported to be involved in t...

journal_title：Bioscience reports

authors： Zhu X,Yu H,Li B,Quan J,Zeng Z,Li G,Liu J

更新日期：2019-11-29 00:00:00

abstract：:Cryptococcus neoformans is an opportunistic fungal pathogen that causes life-threatening meningitis primarily in immunocompromised individuals. In order to survive and proliferate during infection, C. neoformans must adapt to a variety of stresses it encounters within the host. Patient outcome depends on the interacti...

journal_title：Bioscience reports

authors： Altamirano S,Jackson KM,Nielsen K

更新日期：2020-10-30 00:00:00

abstract：:Long non-coding RNAs (lncRNA) CASC2 is a key player in cancer biology. Our new findings showed that both lncRNA CASC2 and IL-17 were up-regulated in plasma of osteoarthritis patients. Plasma levels of lncRNA CASC2 and IL-17 were significantly and positive correlated only in osteoarthritis patients. Overexpression of l...

journal_title：Bioscience reports

authors： Huang T,Wang J,Zhou Y,Zhao Y,Hang D,Cao Y

更新日期：2019-05-14 00:00:00

abstract：:Calpain-1 and calpain-2 are highly structurally similar isoforms of calpain. The calpains, a family of intracellular cysteine proteases, cleave their substrates at specific sites, thus modifying their properties such as function or activity. These isoforms have long been considered to function in a redundant or comple...

journal_title：Bioscience reports

authors： Shinkai-Ouchi F,Shindo M,Doi N,Hata S,Ono Y

更新日期：2020-11-27 00:00:00

abstract：:Breast cancer remains a leading cause of cancer-related mortality in women. In recent years, regulation of genes involved in heparan sulphate (HS) biosynthesis have received increased interest as regulators of breast cancer cell adhesion and invasion. The exostosin (EXT) proteins are glycosyltransferases involved in e...

journal_title：Bioscience reports

authors： Sembajwe LF,Katta K,Grønning M,Kusche-Gullberg M

更新日期：2018-08-31 00:00:00

abstract：:Cervical carcinoma is the most common gynecological cancer in women worldwide. Emerging evidence has shown that long non-coding RNAs (lncRNAs) participate in multiple biological processes of cervical carcinoma tumorigenesis. We aimed to investigate the function of a novel lncRNA RP11-284F21.9 in cervical carcinoma. We...

journal_title：Bioscience reports

authors： Han HF,Chen Q,Zhao WW

更新日期：2020-09-30 00:00:00

abstract：:Lung surfactant protein A (SP-A) is the most abundant surfactant-associated protein present in the lung. A receptor for SP-A has been shown to be present on A549 alveolar type II cells and on other cell types, including alveolar macrophage. The SP-A receptor on A549 cells has been identified as the collection receptor...

journal_title：Bioscience reports

authors： Malhotra R,Haurum J,Thiel S,Jensenius JC,Sim RB

更新日期：1993-04-01 00:00:00

abstract：OBJECTIVE:The role of lncRNAs in tumor has been widely concerned. The present study took HAS2-AS1 (the antisense RNA 1 of HAS2) as a starting point to explore its expression in glioma and its role in the process of migration and invasion, providing a strong theoretical basis for mining potential therapeutic targets of ...

journal_title：Bioscience reports

authors： Wang J,Gu J,You A,Li J,Zhang Y,Rao G,Ge X,Zhang K,Li J,Liu X,Wang Q,Lin T,Cheng L,Zhu M,Wu X,Wang D

更新日期：2020-08-28 00:00:00

abstract：:Many miRNAs play critical roles in modulating various biological processes of osteoclast differentiation and function. Microphthalmia-associated transcription factor (MITF), a target of miR-340, served as pivotal transcription factor involved in osteoclast differentiation. However, the role of miR-340 and MITF during ...

journal_title：Bioscience reports

authors： Zhao H,Zhang J,Shao H,Liu J,Jin M,Chen J,Huang Y

更新日期：2017-07-20 00:00:00

abstract：BACKGROUND:Annulus fibrosus (AF) is important to confine disc nucleus pulposus (NP) tissue during mechanical load experience. However, the knowledge on AF cell biology under mechanical load is much limited compared with disc NP. OBJECTIVE:The present study aimed to investigate responses of apoptosis and matrix metabol...

journal_title：Bioscience reports

authors： Jiang Y,Fu L,Song Y

更新日期：2019-02-22 00:00:00

abstract：:Blood platelets have the capacity to participate in a number of physiological as well as pathological processes within the circulation. In order to evaluate their cellular reactivity a number of platelet function tests have been developed. The main in vitro function tests are assessment of aggregation and adhesion, se...

journal_title：Bioscience reports

authors： Spangenberg P,Till U

更新日期：1989-06-01 00:00:00

abstract：:FAH domain containing protein 1 (FAHD1) is a mammalian mitochondrial protein, displaying bifunctionality as acylpyruvate hydrolase (ApH) and oxaloacetate decarboxylase (ODx) activity. We report the crystal structure of mouse FAHD1 and structural mapping of the active site of mouse FAHD1. Despite high structural simila...

journal_title：Bioscience reports

authors： Weiss AKH,Naschberger A,Cappuccio E,Metzger C,Mottes L,Holzknecht M,von Velsen J,Bowler MW,Rupp B,Jansen-Dürr P

更新日期：2020-03-27 00:00:00

abstract：:3D (three-dimensional) cultures are considered to be an effective method for toxicological studies; however, little evidence has been reported whether 3D cultures have an impact on hepatocellular physiology regarding lipid or glucose metabolism. In the present study, we conducted physiological characterization of hepa...

journal_title：Bioscience reports

authors： Takahashi Y,Hori Y,Yamamoto T,Urashima T,Ohara Y,Tanaka H

更新日期：2015-05-07 00:00:00

abstract：:It is well known that the amino acids in the blood are distributed between the plasma and inside the cells. This study was conducted to determine whether amino acids can be located adsorbed on blood cell membranes. The amino acid concentration in the deproteinized haemolysed blood was higher than that in the fraction ...

journal_title：Bioscience reports

authors： Picó C,Pons A,Palou A

更新日期：1991-08-01 00:00:00

abstract：:Corneal inflammation or keratitis is a significant cause of ocular morbidity around the world. Fortunately, the majority of the cases are successfully managed with medical therapy, but the failure of therapy does occur, leading to devastating consequences of either losing the vision or the eye. This review attempts to...

journal_title：Bioscience reports

authors： Sharma S

更新日期：2001-08-01 00:00:00

abstract：:The hematopoietic cell kinase (HCK), a member of the Src family protein-tyrosine kinases (SFKs), is primarily expressed in cells of the myeloid and B lymphocyte lineages. Nevertheless, the roles of HCK in glioblastoma (GBM) remain to be examined. Thus, we aimed to investigate the effects of HCK on GBM development both...

journal_title：Bioscience reports

authors： Wang Z,Ying C,Zhang A,Xu H,Jiang Y,Lou M

更新日期：2020-06-26 00:00:00

abstract：AIM:IgA nephropathy (IgAN) is one of the most common chronic glomerulonephritis. Its etiology and pathogenesis remain unclear. We thus explored the immune repertoire of the B-cell receptor (BCR) and the heavy-chain complementarity-determining region 3 (CDR3) in renal tissue and peripheral blood of IgAN patients. METHO...

journal_title：Bioscience reports

authors： Chen D,Zhang Z,Yang Y,Hong Q,Li W,Zhuo L

更新日期：2019-10-30 00:00:00

abstract：:Endothelin-1 (ET-1) and plasminogen activator inhibitor-1 (PAI-1) play important roles in pulmonary hypertension (PH) in sickle cell disease (SCD). Our previous studies show higher levels of placenta growth factor (PlGF) in SCD correlate with increased plasma levels of ET-1, PAI-1, and other physiological markers of P...

journal_title：Bioscience reports

authors： Gonsalves CS,Li C,Malik P,Tahara SM,Kalra VK

更新日期：2015-10-12 00:00:00

abstract：BACKGROUND/AIMS:MicroRNAs (miRNAs) are involved in the pathogenesis of osteoarthritis (OA). The present study aimed to investigate the potential function of miR-122 in the development of OA and its potential molecular mechanisms. METHODS:The expression of miR-122, silent information regulator 1 (SIRT1), collagen II, a...

journal_title：Bioscience reports

authors： Bai Y,Chen K,Zhan J,Wu M

更新日期：2020-06-26 00:00:00

abstract：:Cervical cancer (CC) is ranked as the fourth most common cancer that occurs in women universally, which normally causes pain in the lower belly. Plenty of studies have stated that the expression of long non-coding RNAs (lncRNAs) is linked to the cellular development of many kinds of cancers. DSCAM-AS1 has been reporte...

journal_title：Bioscience reports

authors： Liang J,Zhang S,Wang W,Xu Y,Kawuli A,Lu J,Xiu X

更新日期：2020-01-31 00:00:00

abstract：:HIV-1 infects host cells by sequential interactions of its fusion protein (gp120-gp41) with receptors CD4, CXCR4 and/or CCR5 followed by fusion of viral and host membranes. Studies indicate that additional factors such as receptor density and composition of viral and cellular lipids can dramatically modulate the fusio...

journal_title：Bioscience reports

authors： Rawat SS,Johnson BT,Puri A

更新日期：2005-10-01 00:00:00

abstract：:Clustered Regularly Interspaced Short Palindromic Repeat activation-synergistic activation mediator system (CRISPRa-SAM) has been efficiently used to up-regulate the targeted genes in human and mouse. But it is not known whether the CRISPRa-SAM system can be used against porcine disease because its two important trans...

journal_title：Bioscience reports

authors： Jiang J,Sun Y,Xiao R,Wai K,Ahmad MJ,Khan FA,Zhou H,Li Z,Zhang Y,Zhou A,Zhang S

更新日期：2019-08-19 00:00:00

abstract：:For improving Ostwald's viscometry, which is time-consuming and requires a relatively large volume of specimen to determine viscosity, we developed a capillary microviscometric method with an appropriate mathematical model, and have compared this method with Ostwald's method. ...

journal_title：Bioscience reports

authors： Masuhara M,Yokoyama H,Tatsumi N

更新日期：1983-02-01 00:00:00

abstract：:Permeant cationic fluorescent probes are widely employed to monitor mitochondrial transmembrane potential and its changes. The application of such potential-dependent probes in conjunction with both fluorescence microscopy and fluorescence spectroscopy allows the monitoring of mitochondrial membrane potential in indiv...

journal_title：Bioscience reports

authors： Solaini G,Sgarbi G,Lenaz G,Baracca A

更新日期：2007-06-01 00:00:00

abstract：:The cytosolic Ca2+ activity of insulin-releasing clonal cells (RINm5F) was studied with the intracellular fluorescent indicator quin-2. When the extracellular Ca2+ concentration was 1 mM, the basal cytosolic Ca2+ activity was 101 +/- 5 nM. Depolarization with 25 mM K+ increased this Ca2+ activity to at least 318 nM, a...

journal_title：Bioscience reports

authors： Rorsman P,Berggren PO,Gylfe E,Hellman B

更新日期：1983-10-01 00:00:00

abstract：:This paper presents a short review of our contribution to the knowledge of the structure and function of human C1r, the activation unit of C1, the first component of the classical pathway of complement. On the basis of the domain structure of C1r, a model accounting for its autolytic activation mechanism is proposed. ...

journal_title：Bioscience reports

authors： Arlaud GJ,Colomb MG,Villiers CL

更新日期：1985-10-01 00:00:00

abstract：:Wilms tumor is the most common renal malignancy that occurs in children. TP53 gene is considered as a tumor-suppressing gene through controlling cell growth. TP53 gene rs1042522 C>G (Arg72Pro) polymorphism is widely investigated in various types of cancers. However, it is not established if TP53 rs1042522 C>G polymorp...

journal_title：Bioscience reports

authors： Liu P,Zhuo Z,Li W,Cheng J,Zhou H,He J,Zhang J,Wang J

更新日期：2019-01-22 00:00:00