Abstract:
:Permeant cationic fluorescent probes are widely employed to monitor mitochondrial transmembrane potential and its changes. The application of such potential-dependent probes in conjunction with both fluorescence microscopy and fluorescence spectroscopy allows the monitoring of mitochondrial membrane potential in individual living cells as well as in large population of cells. These approaches to the analysis of membrane potential is of extremely high value to obtain insights into both the basic energy metabolism and its dysfunction in pathologic cells. However, the use of fluorescent molecules to probe biological phenomena must follow the awareness of some principles of fluorescence emission, quenching, and quantum yield since it is a very sensitive tool, but because of this extremely high sensitivity it is also strongly affected by the environment. In addition, the instruments used to monitor fluorescence and its changes in biological systems have also to be employed with cautions due to technical limits that may affect the signals. We have therefore undertaken to review the most currently used analytical methods, providing a summary of practical tips that should precede data acquisition and subsequent analysis. Furthermore, we discuss the application and feasibility of various techniques and discuss their respective strength and weakness.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Solaini G,Sgarbi G,Lenaz G,Baracca Adoi
10.1007/s10540-007-9033-4subject
Has Abstractpub_date
2007-06-01 00:00:00pages
11-21issue
1-3eissn
0144-8463issn
1573-4935journal_volume
27pub_type
杂志文章,评审abstract:BACKGROUND:Emerging evidences have indicated that long non-coding RNAs (LncRNAs) play vital roles in cancer development and progression. Previous studies have suggested that overexpression of SPRY4 intronic transcript 1 (SPRY4-IT1) predicates poor prognosis and promotes tumor progress in cervical cancer (CC). However, ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181339
更新日期:2019-06-04 00:00:00
abstract::Isoliquiritigenin (ISL), a natural product isolated from licorice root, exhibits anti-gastric cancer effects. However, applications of ISL are still limited in clinical practice due to its poor bioavailability. To discovery of more effective anti-gastric cancer agents based on ISL, aldol condensation reaction was appl...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20201199
更新日期:2020-06-26 00:00:00
abstract::Conflicting opinions were recently expressed concerning the possible effect of alpha 2-adrenergic agonists upon cyclic AMP production in pancreatic islets. In the present study, clonidine inhibited glucose-induced insulin release from rat pancreatic islets, this inhibitory effect being abolished by idazoxan. Clonidine...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122227
更新日期:1984-06-01 00:00:00
abstract::This article discusses how changes in luminal calcium concentration affect calcium release rates from triad-enriched sarcoplasmic reticulum vesicles, as well as single channel opening probability of the ryanodine receptor/calcium release channels incorporated in bilayers. The possible participation of calsequestrin, o...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/BF01788370
更新日期:1995-10-01 00:00:00
abstract::Chemical transformations, like osmotic translocations, are transport processes when looked at in detail. In chemiosmotic systems, the pathways of specific ligand conduction are spatially orientated through osmoenzymes and porters in which the actions of chemical group, electron and solute transfer occur as vectorial (...
journal_title:Bioscience reports
pub_type:
doi:10.1007/s10540-005-2739-2
更新日期:2004-08-01 00:00:00
abstract:BACKGROUND:This research aimed to study the associations between XPD (G751A, rs13181), hOGG1 (C326G, rs1052133) and XRCC4 (G1394T, rs6869366) gene polymorphisms and the risk of colorectal cancer (CRC) in a Chinese Han population. METHOD:A total of 225 Chinese Han patients with CRC were selected as the study group, and...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181074
更新日期:2019-01-15 00:00:00
abstract::Bladder cancer is a common malignant tumour worldwide. Epithelial-mesenchymal transition (EMT)-related biomarkers can be used for early diagnosis and prognosis of cancer patients. To explore, accurate prediction models are essential to the diagnosis and treatment for bladder cancer. In the present study, an EMT-relate...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20203944
更新日期:2021-01-29 00:00:00
abstract::Glioblastomas (GBMs) are a lethal class of brain cancer, with a median survival <15 months in spite of therapeutic advances. The poor prognosis of GBM is largely attributed to acquired chemotherapy resistance, and new strategies are urgently needed to target resistant glioma cells. Here we report a role for miR-299-5p...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181051
更新日期:2018-09-12 00:00:00
abstract::Growing evidence has uncovered that SLC34A2 plays an evident role in the progression in several types of tumors. However, the biological function and underlying molecular mechanisms of SLC34A2 remain largely unknown. Here, we indicated that SLC34A2 expression was markedly increased in SW480 and HT29 cell line cells co...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180268
更新日期:2019-05-21 00:00:00
abstract::Partitioning in charge-sensitive dextran-poly(ethylene glycol) aqueous phase systems reveals that fixation with even small concentrations of glutaraldehyde (e.g., 0.1% w/v) changes the surface properties of cells. While fixation with larger concentrations of glutaraldehyde (i.e., 1.85%) increases erythrocyte partition...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114811
更新日期:1989-12-01 00:00:00
abstract::Emerging evidence are accumulating that long noncoding RNAs (lncRNAs) have recently been identified to participate in various cellular processes. Terminal differentiation induced ncRNA (TINCR) is a newly identified lncRNA with its functional roles not fully elucidated in human malignancy. The current study aims to ide...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170301
更新日期:2017-07-27 00:00:00
abstract::The hematopoietic cell kinase (HCK), a member of the Src family protein-tyrosine kinases (SFKs), is primarily expressed in cells of the myeloid and B lymphocyte lineages. Nevertheless, the roles of HCK in glioblastoma (GBM) remain to be examined. Thus, we aimed to investigate the effects of HCK on GBM development both...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200975
更新日期:2020-06-26 00:00:00
abstract::Cathepsin D occurs in two forms, a single polypeptide chain (Mr 44 000) and a non-covalent complex of two peptides of Mr 14 000 and 30 000 that is derived by proteolytic processing of the 44 000 polypeptide. The two forms from bovine spleen are closely similar in secondary structure content, in aromatic amino acid env...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119908
更新日期:1985-10-01 00:00:00
abstract::Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb) which has been threatening global public health for many years. High genetic diversity is dominant feature of Mtb. Increasing cases of multidrug-resistant (MDR) tuberculosis (MDR-TB) is a serious public health problem to TB control i...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181746
更新日期:2019-05-31 00:00:00
abstract::Alterations in genomic CpG methylation patterns have been found to be associated with cell transformation and neoplasia. Although it is recognized that methylation of CpG residues negatively regulates gene expression, how the various MBPs (methyl-binding proteins) contribute to this process remains elusive. To determi...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20080032
更新日期:2008-12-01 00:00:00
abstract::Aspergillus fumigatus is the causative agent of IA (invasive aspergillosis) in immunocompromised patients. It possesses a cell wall composed of chitin, glucan and galactomannan, polymeric carbohydrates synthesized by processive glycosyltransferases from intracellular sugar nucleotide donors. Here we demonstrate that A...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20130053
更新日期:2013-09-04 00:00:00
abstract::The cDNA encoding about half of an antigenic non-surface schistosome parasite protein of Mr 97 K has recently been cloned and sequenced (Lanar, Pearce, James and Sher (1986) Science 234:593-596). Analysis of this sequence, together with the properties of the native protein, reveals that this protein is paramyosin, the...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122722
更新日期:1987-01-01 00:00:00
abstract::Pulmonary arterial hypertension (PAH) is a life-threatening chronic cardiopulmonary disorder. However, studies providing PAH-related gene expression profiles are scarce. To identify hub genes involved in PAH, we investigate two microarray data sets from gene expression omnibus (GEO). A total of 150 differentially expr...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20202346
更新日期:2020-09-30 00:00:00
abstract:RATIONALE:Houshiheisan (HSHS), a classic prescription in traditional Chinese medicine (TCM), has remarkable efficacy in the treatment of ischemic stroke. OBJECTIVE:To investigate the pro-angiogenic effect and molecular mechanism of HSHS for stroke recovery. METHODS AND RESULTS:The rat permanent middle cerebral artery...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20191006
更新日期:2019-10-30 00:00:00
abstract::Ehrlich Ascites Tumour cells were irradiated with UV-light to crosslink DNA to proteins in vivo. The DNA fragments associated with the nuclear lamina were purified and characterized. The results of the Cot analysis and the hybridization experiments suggest that the DNA fragments attached to the nuclear lamina although...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119802
更新日期:1989-10-01 00:00:00
abstract::Huaier Granule, a type of traditional Chinese biomedical preparation (TCBP), is considered to be a promising adjuvant therapy for breast cancer. Although an analysis of the published literature has been performed, the exact effects and safety of Huaier Granule remains controversial. Therefore, a wide-ranging systemati...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20202509
更新日期:2020-08-28 00:00:00
abstract::Four days of fasting in the rat reduced brown-adipose-tissue (BAT) mass, mitochondrial protein, and tissue protein content. Specific binding of guanosine diposphate (GDP) to BAT mitochondria was depressed by 55% in 4d-fasted rats. Rats fasted for 3 d, and then refed a single carbohydrate meal (40 kJ), showed a signifi...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01140499
更新日期:1984-04-01 00:00:00
abstract::Brain cells dissociated from 15-day-old embryonic mice and grown in culture contain both cytosolic and nuclear receptors for L-3,5,3'-triiodothyronine (L-T3). KD values for L-T3 of 3.05 X 10(-9) M and 4.2 X 10(-9) M were determined with the cytosolic and nuclear receptors respectively. These cultured cells, which are ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114868
更新日期:1981-04-01 00:00:00
abstract::The increase in % phosphorylase a associated with muscle contraction is reversed after a few minutes despite continued contractile activity. The present study was undertaken to determine whether adrenaline can activate phosphorylase after deactivation has occurred during prolonged stimulation of contraction. We found ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01115118
更新日期:1982-05-01 00:00:00
abstract::The cellular onc-genes c-src and c-yes are expressed very differently during chicken embryonic development. The c-src mRNA and its translational product are detectable at high levels in brain extracts of chicken embryos and adult chickens, whereas muscle extracts show an age-dependent decrease in the amounts of c-src-...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01128817
更新日期:1984-09-01 00:00:00
abstract::This study is part of an attempt to understand the role of specific cellular activities in the bone resorptive process. Experiments were performed whereby known pharmacological agents were used to inhibit individual modes of osteoclastic activity, such as motility and secretion. The effects of such treatments on bone ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116615
更新日期:1990-12-01 00:00:00
abstract::The present study aimed to evaluate what dosage of gold nanoparticles (GNPs) would improve growth performance, antioxidant levels and immune defense in broiler chickens. The experiment was carried out on 90 one-day-old mixbred Cobb chicks. The birds were allocated into three groups with three replicates. Group (1) kep...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20194296
更新日期:2020-03-27 00:00:00
abstract::Facioscapulohumeral muscular dystrophy (FSHD) region gene 1 (FRG1) is a candidate gene for FSHD. FRG1 regulates various muscle-related functions, but studies have proposed its role in development and angiogenesis also, where it is involved with tumor-associated molecules. Therefore, we decided to look into its role in...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20171062
更新日期:2017-10-27 00:00:00
abstract::Stimulatory GTP-binding Protein (Gs) and adenylate cyclase prepared from bovine brain cortices were co-reconstituted into asolectin vesicles with or without 1000-fold transmembrane Ca2+ gradient. The results showed that both basal activity and Gs-stimulated activity of adenylate cyclase were highest in proteoliposomes...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01200247
更新日期:1994-08-01 00:00:00
abstract::Capacity to synthesize glucose, urea, and ketone bodies is well maintained in hepatocytes after storage for at least 24 h at 4 degrees C. Substrates and albumin are the only requirements. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01120697
更新日期:1983-06-01 00:00:00