Abstract：
BACKGROUND:Emerging evidences have indicated that long non-coding RNAs (LncRNAs) play vital roles in cancer development and progression. Previous studies have suggested that overexpression of SPRY4 intronic transcript 1 (SPRY4-IT1) predicates poor prognosis and promotes tumor progress in cervical cancer (CC). However, the underlying mechanism of SPRY4-IT1 in CC remains unknown. The aim of the present study is to evaluate the function and mechanism of SPRY4-IT1 in CC. METHODS:SPRY4-IT1 was detected by quantitative PCR. Wound-healing assay and Transwell assay were performed to detect cell migration and invasion, respectively. Western blotting assays were used to analyze the protein expression of E-cadherin, N-cadherin and vimentin. Tumor xenografts experiments were performed to detect the effect of SPRY4-IT1 in vivo. Dual luciferase reporter assay was used to investigate potential molecular mechanism of SPRY4-IT1 in CC cells. RESULTS:SPRY4-IT1 was up-regulated in CC cell lines. Knockdown of SPRY4-IT1 significantly inhibited CC cells migration and invasion in vitro and in vivo Moreover, knockdown of SPRY4-IT1 significantly suppressed the epithelial-mesenchymal transition (EMT) of CC by increased E-cadherin expression and decreased the N-cadherin and vimentin expression. Mechanically, SPRY4-IT1 could directly bind to miR-101-3p and effectively act as a competing endogenous RNA (ceRNA) for miR-101-3p to regulate the expression of the target gene ZEB1Conclusions: Our findings indicate that the SPYR4-IT1/miR-101-3p/ZEB1 axis contributes to CC migration and invasion, which may provide novel insights into the function of lncRNA-driven tumorigenesis of CC.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Fan MJ,Zou YH,He PJ,Zhang S,Sun XM,Li CZdoi
10.1042/BSR20181339subject
Has Abstractpub_date
2019-06-04 00:00:00issue
6eissn
0144-8463issn
1573-4935pii
BSR20181339journal_volume
39pub_type
杂志文章abstract：:Rodney Porter's separation of antibody molecules into Fab and Fc fragments engendered the notion that a single antibody polypeptide chain might be coded by two or more genes. This concept profoundly influenced the development of molecular immunology over the past 25 years. Our current knowledge of antibody gene organi...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01119901
更新日期：1985-10-01 00:00:00
abstract：:The human bronchial epithelial cell line, 16HBE14o- (16HBE), is widely used as a model for respiratory epithelial diseases and barrier function. During differentiation, transepithelial electrical resistance (TER) increased to approximately 800 Ohms × cm2, while 14C-d-mannitol flux rates (Jm) simultaneously decreased. ...
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abstract：:The Gram-positive bacterium Staphylococcus pseudintermedius is a leading cause of canine bacterial pyoderma, resulting in worldwide morbidity in dogs. S. pseudintermedius also causes life-threatening human infections. Furthermore, methicillin-resistant S. pseudintermedius is emerging, resembling the human health threa...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20140088
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abstract：:Thrombospondin-2 (THBS2) is a secreted protein overexpressed in numerous cancers and may function as a diagnostic tumor marker. The objective of the present study was to investigate the diagnostic performance of serum THBS2 in early stage non-small-cell lung cancer (NSCLC). Serum THBS2 and Cyfra21-1 level were evaluat...
journal_title：Bioscience reports
pub_type： 临床试验,杂志文章
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abstract：:Escherichia coli accumulates K+ by means of multiple transport systems, of which TrkA is the most prominent at neutral and alkaline pH while Kup is major at acidic pH. In the present study, K+ uptake was observed with cells grown under fermentative conditions at an initial pH of 9.0 and 7.3 (the medium pH decreased to...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1023/a:1026493024066
更新日期：2000-08-01 00:00:00
abstract：:Levels of red cell, serum acid, and alkaline phosphatases, serum amylase, alanine and aspartate transferase and bilirubin were examined in streptozotocin-induced diabetic rats treated with garlic oil and compared with the corresponding levels in diabetic control rats, normal rats and normal rats on garlic oil. Values ...
journal_title：Bioscience reports
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doi：10.1023/a:1010425932561
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abstract：:The rate of efflux of L-glutamate from renal brush-border membrane vesicles was enhanced by Na+ and by extravesicular L-glutamate, but not by D-glutamate nor analogs of L-glutamate that do not share the Na+-L-glutamate co-transport system. These results suggest that efflux was mediated by the Na+-L-glutamate carrier. ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01116468
更新日期：1981-09-01 00:00:00
abstract：:The concentration of carbonic anhydrase III (CAIII) in male rat liver was found to be 30 times greater than that in the female. Castration of male rats led to marked reduction in liver CAIII concentrations which could be partially restored to control levels by testosterone replacement. Marked developmental and senesce...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01121959
更新日期：1983-05-01 00:00:00
abstract：:The proton conductance of the mitochondrial inner membrane can be quantified by applying Ohm's law to the experimentally determined protonmotive force and the proton current flowing around the proton circuit in the absence of ATP synthesis or ion transport. This last parameter is derived from the rate of State 4 respi...
journal_title：Bioscience reports
pub_type： 杂志文章,评审
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journal_title：Bioscience reports
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doi：10.1007/BF01121952
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abstract：:Purpose: Wilson's disease (WD) is a genetic disorder of copper metabolism with pathological copper accumulation in the brain. The purpose of the present study was to evaluate the relationship between the damaged white matter and the impaired cognitive function in WD patients. Materials and methods: Thirty WD adolescen...
journal_title：Bioscience reports
pub_type： 杂志文章
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更新日期：2019-03-15 00:00:00
abstract：:BRL 26830 is a thermogenic beta-adrenoceptor agonist which stimulates lipolysis and fatty acid oxidation in vivo. It also stimulates insulin secretion, and hence promotes glucose utilisation in vivo. The effect of this agent on white and brown adipose tissue of the rat was investigated. BRL 26830 increased the rate of...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01117517
更新日期：1989-02-01 00:00:00
abstract：:Keratin 18 (KRT18) has been suggested to be overexpressed in most types of human tumor, but the expression pattern of KRT18 in colorectal cancer (CRC) remained unknown. In our research, KRT18 protein expression was markedly increased in CRC cancer tissues and cell lines compared with adjacent normal colorectal tissues...
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pub_type： 杂志文章
doi：10.1042/BSR20190884
更新日期：2019-08-13 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章,评审
doi：10.1042/BSR20190377
更新日期：2019-08-02 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20080145
更新日期：2009-08-01 00:00:00
abstract：:The role of the topoisomerase enzyme in DNA recombination was investigated by extracting chromosomal deoxyribonucleoproteins from a variety of cultured mammalian cells and assaying for the formation of recombinant DNA structures. Although each of the crude deoxyribonucleoprotein preparations contained topoisomerase ac...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01115252
更新日期：1982-07-01 00:00:00
abstract：:The kinetics of the fusion process between erythrocyte ghosts, as induced by Sendai virus, were readily revealed by a simple fluorescence procedure previously employed to characterize the fusion of viruses with biological membranes. The method relies on the relief of fluorescence selfquenching of the membrane-inserted...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01114971
更新日期：1986-11-01 00:00:00
abstract：:Circulating tumor cells can provide important diagnostic and prognostic information of the patients with non-small cell lung cancer (NSCLC). Aldehyde dehydrogenase 1 (ALDH1), a cancer stem cell marker, has been used in various tumors, including NSCLC. In the present study, we isolated the circulating ALDH1+ tumor cell...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20180914
更新日期：2018-10-31 00:00:00
abstract：:Glycine methyl ester, an inhibitor of transglutaminase, decreased glucose-stimulated insulin release and delayed proinsulin conversion in rat pancreatic islets pulse-labelled with L-[4-3H]phenylalanine. Sarcosine methyl ester, which does not inhibit transglutaminase activity, failed to affect insulin release and proin...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01117071
更新日期：1985-07-01 00:00:00
abstract：:We aimed to determine the effect of 'Xiaozeng No. 1' (XZ-1) on cellular apoptosis changes of gastric epithelial dysplasia (GED) and to explore the underlying mechanism. Specimens taken from the pyloric area of the stomachs from rats in each group were subjected to Hematoxylin and Eosin (H&E) staining for pathological ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20171529
更新日期：2018-06-12 00:00:00
abstract：:125I-insulin was shown to be internalized in vivo to a discrete population of low-density membranes (ligandosomes), distinct from the +Golgi, endoplasmic reticulum, plasma membrane, and lysosomes. However, analytical subcellular fractionation shows that glutathione-insulin transhydrogenase is localized to the endoplas...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01122896
更新日期：1983-04-01 00:00:00
abstract：:Prostate cancer (PCa), the second most mortal cancer from developed countries, presents a high level of chemoresistance. There is emerging evidence underscores the critical role of autophagy in the onset, progression, and chemoresistance of PCa. In the present study, we investigated the possible role of a novel autoph...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20170770
更新日期：2019-08-23 00:00:00
abstract：:A few studies suggested the contribution of PPARs to the etiology of schizophrenia (SCZ). However, it is still not clear whether variants in PPAR-encoding genes have a direct association with SCZ. The potential linkage between SCZ and the variants within PPAR encoding genes (PPARA, PPARD, and PPARG) was tested in a la...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20201083
更新日期：2020-07-31 00:00:00
abstract：BACKGROUND:This research aimed to study the associations between XPD (G751A, rs13181), hOGG1 (C326G, rs1052133) and XRCC4 (G1394T, rs6869366) gene polymorphisms and the risk of colorectal cancer (CRC) in a Chinese Han population. METHOD:A total of 225 Chinese Han patients with CRC were selected as the study group, and...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20181074
更新日期：2019-01-15 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20191496
更新日期：2019-08-19 00:00:00
abstract：:Mice and rats are animals commonly used in research and laboratory testing. Compared with other animal species, they harbor many more zoonotic agents. Hymenolepis nana (H. nana) is a common tapeworm that parasitizes both humans and rodents. Although this tapeworm is of socio-economic importance worldwide, information ...
journal_title：Bioscience reports
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doi：10.1042/BSR20181224
更新日期：2019-02-08 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20170302
更新日期：2017-07-20 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01128817
更新日期：1984-09-01 00:00:00
abstract：:MicroRNAs (miRNAs) have been frequently identified as key mediators in almost all developmental and pathological processes, including those in the liver. The present study was conducted with aims of investigating the role of microRNA-138 (miR-138) in acute liver failure (ALF) via a mechanism involving p53 and liver ma...
journal_title：Bioscience reports
pub_type： 杂志文章
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更新日期：2019-07-18 00:00:00
abstract：:Osteoarthritis (OA) is the most common form of arthritis, and connective tissue growth factor (CTGF) is found to be up-regulated in adjacent areas of cartilage surface damage. CTGF is present in osteophytes of late stage OA. In the present study, we have reviewed association of CTGF in the development and progression ...
journal_title：Bioscience reports
pub_type： 杂志文章,评审
doi：10.1042/BSR20191374
更新日期：2019-07-18 00:00:00
