Abstract:
:Prostate cancer (PCa), the second most mortal cancer from developed countries, presents a high level of chemoresistance. There is emerging evidence underscores the critical role of autophagy in the onset, progression, and chemoresistance of PCa. In the present study, we investigated the possible role of a novel autophagy regulator, activating molecule in beclin1-regulated autophagy1 (Ambra1), a novel ATG gene in the sensitivity or PCa cells to cisplatin. We explored the regulation by the Ambra1 manipulation on the induction of apoptosis and autophagy in human PCa DU145 cells in the presence of cisplatin, via up- or down-regulating Ambra1 expression. In addition, we examined the colony forming of DU145 cells post cisplatin treatment and Ambra1 manipulation. Our results demonstrated that the Ambra1 up-regulation reduced, whereas Ambra1 knockdown increased the cisplatin-induced apoptosis, caspase 3 cleavage, and poly ADP-ribose polymerase (PARP) cleavage. Interestingly, we also found significant autophagy induction in the cisplatin-treated DU145 cells, with increased autophagic vesicles, up-regulated autophagy-related markers. However, the cisplatin-induced autophagy was up-regulated by the Ambra1 overexpression or was down-regulated by the Ambra1 knockdown. In addition, the colony forming was also positively regulated by Ambra1 in DU145 cells post cisplatin treatment. In conclusion, Ambra1 negatively regulates the cisplatin-induced apoptosis and the cisplatin-mediated growth reduction in DU145 cells, in association with the Ambra1-mediated autophagy promotion. It implies that Ambra1-mediated autophagy might be an important mechanism underlining the sensitivity reduction of PCa cells.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Liu J,Chen Z,Guo J,Wang L,Liu Xdoi
10.1042/BSR20170770subject
Has Abstractpub_date
2019-08-23 00:00:00issue
8eissn
0144-8463issn
1573-4935pii
BSR20170770journal_volume
39pub_type
杂志文章abstract:OBJECTIVE:To study the correlation between adenomatous polyposis coli (APC) gene 3' untranslated region (UTR) single nucleotide polymorphisms (SNPs) and their interactions with environmental factors and the risk of colorectal cancer (CRC) in a Chinese Han population. METHODS:Genotypes of APC gene 3'UTR rs1804197, rs41...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20192429
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01855015
更新日期:1996-08-01 00:00:00
abstract::Significant amounts of somatostatin-like immunoreactivity (SLI) were detected in the extract of a human catecholamine-secreting adrenal medullary tumour. After salt fractionation and reconstitution the major portion of SLI was purified by gel filtration and two HPLC steps; in all three systems it eluted in the positio...
journal_title:Bioscience reports
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doi:10.1007/BF01116377
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abstract::Baicalin is the main bioactive component extracted from the traditional Chinese medicine Baical Skullcap Root, and its anti-tumor activity has been studied in previous studies. PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK), a serine/threonine protein kinase, is highly expressed in many cancer cell...
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doi:10.1042/BSR20160174
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journal_title:Bioscience reports
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doi:10.1007/BF01115011
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116866
更新日期:1987-09-01 00:00:00
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
doi:10.1042/BSR20193172
更新日期:2020-04-30 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1023/a:1020262811459
更新日期:1999-12-01 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20150080
更新日期:2015-06-12 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20191609
更新日期:2020-01-31 00:00:00
abstract::C3G (Crk SH3-domain-binding guanine-nucleotide-releasing factor) is a ubiquitously expressed member of a class of molecules called GEFs (guanine-nucleotide-exchange factor) that activate small GTPases and is involved in pathways triggered by a variety of signals. It is essential for mammalian embryonic development and...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1042/BSR20100094
更新日期:2011-08-01 00:00:00
abstract::Cryogenic transmission electron microscopy (cryo-TEM) is a high-resolution biological imaging method, whereby biological samples, such as purified proteins, macromolecular complexes, viral particles, organelles and cells, are embedded in vitreous ice preserving their native structures. Due to sensitivity of biological...
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doi:10.1042/BSR20170203
更新日期:2018-04-27 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20120124
更新日期:2013-03-13 00:00:00
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pub_type: 杂志文章
doi:10.1007/BF01117013
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pub_type: 杂志文章
doi:10.1042/BSR20182431
更新日期:2020-05-29 00:00:00
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pub_type: 杂志文章
doi:10.1042/BSR20181411
更新日期:2018-12-07 00:00:00
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pub_type: 杂志文章
doi:10.1042/BSR20140015
更新日期:2014-09-17 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119908
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journal_title:Bioscience reports
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doi:10.1042/BSR20160122
更新日期:2016-06-30 00:00:00
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journal_title:Bioscience reports
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doi:10.1042/BSR20140150
更新日期:2015-01-14 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1023/a:1027335217774
更新日期:1997-02-01 00:00:00
abstract::A new method for detecting viral antibodies in cerebrospinal fluid is described. The technique has many advantages over previously published methods in that it is highly sensitive eliminating the need to concentrate the CSF, takes 5 h to complete, avoids the use of radionucleides, and most importantly circumvents prob...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122226
更新日期:1984-06-01 00:00:00
abstract::The effect of carbamylcholine and the calcium ionophore A23187 on catecholamine release and intracellular free calcium, [Ca2+]i, in bovine adrenal chromaffin cells was determined. At 10(-4) M carbamylcholine maximal release occurred with an accompanying increase in [Ca2+]i from a basal level of 168 nM to less than 300...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121918
更新日期:1984-07-01 00:00:00