Abstract:
:The kinetics of the fusion process between erythrocyte ghosts, as induced by Sendai virus, were readily revealed by a simple fluorescence procedure previously employed to characterize the fusion of viruses with biological membranes. The method relies on the relief of fluorescence selfquenching of the membrane-inserted probe octadecyl Rhodamine B chloride (R18) as occurs when labeled membranes fuse with unlabeled counterparts. The kinetics of R18 insertion into ghost membranes, the non-exchangeable properties of the fluorophore and the kinetics, and some characteristics of Sendai virus-induced fusion of ghosts, are described. We propose that the experimental approach may be particularly advantageous to obtain insight into the efficiency and mechanism of a wide range of fusogens, capable of inducing fusion of erythrocyte membranes.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Hoekstra D,Klappe Kdoi
10.1007/BF01114971subject
Has Abstractpub_date
1986-11-01 00:00:00pages
953-60issue
11eissn
0144-8463issn
1573-4935journal_volume
6pub_type
杂志文章abstract::The principle of the dual inhibitor titration method for testing models of electron-transport phosphorylation is outlined, and the method is applied to the study of photophosphorylation in bacterial chromatophores. It is concluded that energy coupling is strictly localized in nature in this system, in the sense that f...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114933
更新日期:1982-10-01 00:00:00
abstract::Diabetes mellitus (DM) is a potential etiology of disc degeneration. Glucagon-like peptide-1 (GLP-1) is currently regarded as a powerful treatment option for type 2 diabetes. Apart from the beneficial effects on glycaemic control, GLP-1 has been reported to exert functions in a variety of tissues on modulation of cell...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190109
更新日期:2019-08-19 00:00:00
abstract::Liver cancer is now one of the most lethal and commonest cancers in the world, among which over 90% is hepatocellular carcinoma (HCC). Recent studies have confirmed long non-coding RNAs (lncRNAs) are implicated in carcinogenesis. It has been reported lncRNA LINC00668 serves as an oncogene in several cancers. However, ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20192697
更新日期:2020-05-29 00:00:00
abstract::Neuroblastoma, which accounts for approximately 10% of all pediatric cancer-related deaths, has become a therapeutic challenge and global burden attributed to poor outcomes and mortality rates of its high-risk form. Previous genome-wide association studies (GWASs) identified the LINC00673 rs11655237 C>T polymorphism t...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20171667
更新日期:2018-02-08 00:00:00
abstract::Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb) which has been threatening global public health for many years. High genetic diversity is dominant feature of Mtb. Increasing cases of multidrug-resistant (MDR) tuberculosis (MDR-TB) is a serious public health problem to TB control i...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181746
更新日期:2019-05-31 00:00:00
abstract:BACKGROUND:Breast cancer (BC) is the most common malignancy among females worldwide. The tumor microenvironment usually prevents effective lymphocyte activation and infiltration, and suppresses infiltrating effector cells, leading to a failure of the host to reject the tumor. CC chemokines play a significant role in in...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20202042
更新日期:2020-11-27 00:00:00
abstract::Artocarpus incisa (breadfruit) seeds contain three different lectins (Frutalin, Frutapin (FTP) and Frutackin) with distinct carbohydrate specificities. The most abundant lectin is Frutalin, an α-D-galactose-specific carbohydrate-binding glycoprotein with antitumour properties and potential for tumour biomarker discove...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170969
更新日期:2017-07-21 00:00:00
abstract::Although wasting marmoset syndrome (WMS) is one of the biggest problems facing captive marmoset colonies, the mechanisms underlying its pathogenesis remain unclear. In our clinical experience, it is difficult to cure WMS-affected marmosets with severe hypoalbuminemia. Thus, the mechanisms underlying hypoalbuminemia in...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190562
更新日期:2019-07-08 00:00:00
abstract::Specific binding sites for somatostatin have been found in the cytosolic fractions of both parietal and non-parietal cells from rabbit gastric fundic mucosa. The stoichiometric data suggested the presence of two classes of binding sites in both types of cells. The number of low-affinity binding sites was significantly...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116904
更新日期:1985-04-01 00:00:00
abstract::Two established cancer cell lines, MCF-7 and Ishikawa, were both obtained directly from a cell repository and through another laboratory. The karyotypes from the two MCF-7 cell lines had up to 83 chromosomes and similarities for chromosomal gain and structural abnormalities. The two Ishikawa cell lines had up to 60 ch...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/s10540-005-2797-5
更新日期:2004-12-01 00:00:00
abstract::LGMD (limb-girdle muscular dystrophy) and CMD (congenital muscular dystrophy) are two common forms of neuromuscular disorders which are distinguishable by their age of onset but with probably a similar underlying pathway. In the present study, we report immunohistochemical, Western-blot and genetic analyses in a large...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20100026
更新日期:2011-04-01 00:00:00
abstract::Sulphate efflux from human placental microvillus membrane vesicles was inhibited by external DIDS (KI congruent to 10(-6) M). This inhibition was partially reversed on addition of the translocated substrates sulphate or selenate to the external medium: selenite which is not translocated does not protect against DIDS i...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116242
更新日期:1986-10-01 00:00:00
abstract::The secretory process is a coordinated cellular response, initiated by occupation of surface receptors and comprising an ordered sequence of biochemical steps subject to multiple controls. Conceptually we can divide the sequence into two main sections comprising early, receptor-mediated events leading to generation of...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/BF01362501
更新日期:1987-05-01 00:00:00
abstract::Obesity is considered a global epidemic. Specifically, obesity during pregnancy programs an increased risk of the offspring developing metabolic disorders in addition to the adverse effects on the mother per se Large numbers of human and animal studies have demonstrated that the gut microbiota plays a pivotal role in ...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1042/BSR20171234
更新日期:2018-04-13 00:00:00
abstract::The effect of sodium butyrate (NaBut) on cell growth was studied in normal rat kidney (NRK) fibroblasts, and in NRK cells stably transfected with either the adenoviral gene E1A (wild-type), or mutated E1A (E1Amut; with a deletion in the CR1 domain), or with the transforming Ha-ras (EJ) gene. The growth of all these ce...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01199053
更新日期:1994-12-01 00:00:00
abstract::Ovarian cancer is one of the most common gynecological cancers with a high mortality rate in females. Chromatin target of protein arginine methyltransferase (CHTOP) is an important intracellular protein that regulates the transcriptional activation of several oncogenic genes in glioblastomagenesis and controls mature ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190016
更新日期:2019-04-16 00:00:00
abstract::To clarify the mechanism of circGOLPH3 regulation on prostate cancer cells, we performed an overexpression and interference circGOLPH3 assay in prostate cancer cells PC-3 and then evaluated cellular viability, proliferation, cell cycle, and apoptosis of prostate cancer cells by MTT, CCK8, Edu stain, TUNEL stain, and f...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200936
更新日期:2020-12-23 00:00:00
abstract::In India, Sorghum plant allergenicity was reported to be approximately 54.9%. Sorghum bicolor Polcalcin (Sorb PC) was identified as the panallergen but the specificity of this allergen is yet to be characterized. The present study was aimed to characterize the antigenic determinants of Sorb PC that are responsible for...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20191835
更新日期:2019-11-29 00:00:00
abstract::Oral squamous cell carcinoma (OSCC) is the most common malignancy in the oral cavity, which accounts for >90% of all diagnosed oral cancers. 2-phenylethynesulfonamide (PES) was known as a selective heat shock protein 70 (Hsp70) function inhibitor, which induced cytotoxic effects on various tumor cell types, but showed...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200079
更新日期:2020-03-27 00:00:00
abstract::The emergence of multidrug-resistant (MDR) microbes caused by overuse of antibiotics leads to urgent demands for novel antibiotics exploration. Our recent data showed that Ly2.1-3 (a novel lymphocyte antigen 6 (Ly6) gene cluster) were proteins with cationic nature and rich in cysteine content, that are characteristic ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160265
更新日期:2017-01-17 00:00:00
abstract::Antimicrobial peptides are a promising resource for developing novel antibiotic and even anticancer drugs. Here, a 28-mer polypeptide, Ranatuerin-2PLx (R2PLx), was identified from lyophilised skin secretions. The chemically synthetic replicates exhibited moderate and broadspectrum antimicrobial effect against various ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180710
更新日期:2018-11-09 00:00:00
abstract::Identified and cloned in 1996 for the first time, G protein-coupled oestrogen receptor (ER) 30 (GPR30/GPER) has been a hot spot in the field of sex hormone research till now. In the present study, we examined the effects of low-dose oestradiol (E2) combined with G15, a specific antagonist of GPR30 on ovariectomy (OVX)...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20150146
更新日期:2015-07-03 00:00:00
abstract::Oral vaccination requires an antigen delivery vehicle to protect the antigen and to enhance translocation of the antigen to the mucosa-associated lymphoid tissue. A variety of antigen delivery vehicles including liposomes have been studied for mucosal immunization. The advantages of liposome formulations are their par...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1023/a:1020103109483
更新日期:2002-04-01 00:00:00
abstract::Perfusion of Langendorff rat hearts with [14C]adenosine yields an acid-insoluble, radioactive product whose concentration falls during ischaemia. The properties of the substance show that it is a polyribonucleotide. It is suggested that it may be mitochondrial poly A acting as a storage form of adenine nucleotides. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117435
更新日期:1985-01-01 00:00:00
abstract::Carnosic acid (CA), a major polyphenolic diterpene present in Rosmarinus officinalis, has been reported to have multiple functions, including antitumor activity. The MTT assay, BrdU incorporation, wound healing, and colony formation were used to detect melanoma B16F10 cell growth and proliferation. Flow cytometry was ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180005
更新日期:2018-07-02 00:00:00
abstract::Cryogenic transmission electron microscopy (cryo-TEM) is a high-resolution biological imaging method, whereby biological samples, such as purified proteins, macromolecular complexes, viral particles, organelles and cells, are embedded in vitreous ice preserving their native structures. Due to sensitivity of biological...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170203
更新日期:2018-04-27 00:00:00
abstract::Stimulatory GTP-binding Protein (Gs) and adenylate cyclase prepared from bovine brain cortices were co-reconstituted into asolectin vesicles with or without 1000-fold transmembrane Ca2+ gradient. The results showed that both basal activity and Gs-stimulated activity of adenylate cyclase were highest in proteoliposomes...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01200247
更新日期:1994-08-01 00:00:00
abstract::This study is part of an attempt to understand the role of specific cellular activities in the bone resorptive process. Experiments were performed whereby known pharmacological agents were used to inhibit individual modes of osteoclastic activity, such as motility and secretion. The effects of such treatments on bone ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116615
更新日期:1990-12-01 00:00:00
abstract::Maclurin, the potent non-specific blood-group hemagglutinin present in extracts of Maclura pomifera, has been purified by a new biospecific affinity-chromatographic procedure. Additional studies have indicated that this hemagglutinin occurs as five closely related tetrameric protein isoforms derived from two non-coval...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01136724
更新日期:1982-04-01 00:00:00
abstract::Fungal endophytes are known to secrete a large array of secondary metabolites (phenols, flavonoids, indole acetic acid (IAA) etc.) that facilitate crops under stress conditions. Considering this, a potent plant growth promoting endophyte (SXSp1) from the spines and leaves of Solanum xanthocarpum L. has been isolated. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190290
更新日期:2019-10-30 00:00:00