Abstract:
:Cathepsin D occurs in two forms, a single polypeptide chain (Mr 44 000) and a non-covalent complex of two peptides of Mr 14 000 and 30 000 that is derived by proteolytic processing of the 44 000 polypeptide. The two forms from bovine spleen are closely similar in secondary structure content, in aromatic amino acid environment and in the two step denaturation behaviour. Enzyme activity is lost irreversibly on denaturation but conformation can be partially regained. The two separated chains will only refold partially and this is related to their positions in the overall structure of cathepsin D. It is suggested that the processing step is related to protein turnover.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Pain RH,Lah T,Turk Vdoi
10.1007/BF01119908subject
Has Abstractpub_date
1985-10-01 00:00:00pages
957-67issue
10-11eissn
0144-8463issn
1573-4935journal_volume
5pub_type
杂志文章abstract::Brain cells dissociated from 15-day-old embryonic mice and grown in culture contain both cytosolic and nuclear receptors for L-3,5,3'-triiodothyronine (L-T3). KD values for L-T3 of 3.05 X 10(-9) M and 4.2 X 10(-9) M were determined with the cytosolic and nuclear receptors respectively. These cultured cells, which are ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114868
更新日期:1981-04-01 00:00:00
abstract::Levels of red cell, serum acid, and alkaline phosphatases, serum amylase, alanine and aspartate transferase and bilirubin were examined in streptozotocin-induced diabetic rats treated with garlic oil and compared with the corresponding levels in diabetic control rats, normal rats and normal rats on garlic oil. Values ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1023/a:1010425932561
更新日期:2001-02-01 00:00:00
abstract::Purpose: Wilson's disease (WD) is a genetic disorder of copper metabolism with pathological copper accumulation in the brain. The purpose of the present study was to evaluate the relationship between the damaged white matter and the impaired cognitive function in WD patients. Materials and methods: Thirty WD adolescen...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181651
更新日期:2019-03-15 00:00:00
abstract::Escherichia coli accumulates K+ by means of multiple transport systems, of which TrkA is the most prominent at neutral and alkaline pH while Kup is major at acidic pH. In the present study, K+ uptake was observed with cells grown under fermentative conditions at an initial pH of 9.0 and 7.3 (the medium pH decreased to...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1023/a:1026493024066
更新日期:2000-08-01 00:00:00
abstract::Heterotopic ossification (HO) is the aberrant formation of mature, lamellar bone in nonosseous tissue. Fibrodysplasia ossificans progressiva (FOP) is a rare and devastating genetic disorder that causes progressive HO in the ligaments, tendons, and muscles throughout the body. FOP is attributed to an autosomal mutation...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1042/BSR20190377
更新日期:2019-08-02 00:00:00
abstract::Stimulatory GTP-binding Protein (Gs) and adenylate cyclase prepared from bovine brain cortices were co-reconstituted into asolectin vesicles with or without 1000-fold transmembrane Ca2+ gradient. The results showed that both basal activity and Gs-stimulated activity of adenylate cyclase were highest in proteoliposomes...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01200247
更新日期:1994-08-01 00:00:00
abstract::Corneal inflammation or keratitis is a significant cause of ocular morbidity around the world. Fortunately, the majority of the cases are successfully managed with medical therapy, but the failure of therapy does occur, leading to devastating consequences of either losing the vision or the eye. This review attempts to...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1023/a:1017939725776
更新日期:2001-08-01 00:00:00
abstract::Oral squamous cell carcinoma (OSCC) is the most common malignancy in the oral cavity, which accounts for >90% of all diagnosed oral cancers. 2-phenylethynesulfonamide (PES) was known as a selective heat shock protein 70 (Hsp70) function inhibitor, which induced cytotoxic effects on various tumor cell types, but showed...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200079
更新日期:2020-03-27 00:00:00
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journal_title:Bioscience reports
pub_type: 评论,信件
doi:10.1042/BSR20180505
更新日期:2018-10-31 00:00:00
abstract::The neurotransmitter, serotonin has emerged as a tumor growth factor and immune response regulator through complex signaling pathways. Yip1 Interacting Factor Homolog B (YIF1B) is a membrane protein involved in serotonin receptor (HTR) membrane trafficking and signal transmission in neuropathy. Participation of YIF1B ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20201384
更新日期:2020-07-31 00:00:00
abstract::Incubation of adult rat cardiac myocytes with increasing glucose concentrations decreased phosphorylation (αThr172) and activity of AMPK (AMP-activated protein kinase). The effect could be demonstrated without measurable changes in adenine nucleotide contents. The glucose effect was additive to the decrease in AMPK ac...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20110075
更新日期:2012-06-01 00:00:00
abstract::The cellular onc-genes c-src and c-yes are expressed very differently during chicken embryonic development. The c-src mRNA and its translational product are detectable at high levels in brain extracts of chicken embryos and adult chickens, whereas muscle extracts show an age-dependent decrease in the amounts of c-src-...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01128817
更新日期:1984-09-01 00:00:00
abstract::N-(2-Hydroxypropyl)methacrylamide copolymers bearing oligopeptide side-chains terminating in p-nitroaniline (NAp) were incubated with rat liver lysosomal enzymes in the presence of the thiol glutathione, and the rate of p-nitroaniline release was measured. Twelve of the 16 side-chains investigated were hydrolysed to r...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122173
更新日期:1982-12-01 00:00:00
abstract::The purpose of this meta-analysis was to evaluate the beneficial and adverse effects of tripterygium glycosides (TGs) combined with angiotensin II receptor blocker (ARB) on diabetic nephropathy (DN). We searched for randomized controlled trials (RCTs) in PubMed, Embase, Cochrane Central Register of Controlled Trials, ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20202391
更新日期:2020-11-27 00:00:00
abstract::Doxorubicin (DOX) resistance in breast cancer largely results from the breast cancer stem cell like cells (BCSCs) which could be targetted to improve the efficacy of chemotherapy. Cell permeabilization using microbubbles (MBs) and ultrasound (US) have the potential for delivering molecules into the cytoplasm. We aim t...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20171137
更新日期:2017-11-09 00:00:00
abstract::We aimed to confirm the role of miR-1296-5p in gastric cancer and to identify its target genes. The expression of miR-1296-5p was measured in gastric cancer tissues and cell lines. The function of miR-1296-5p was examined by the overexpression and inhibition of its expression in typical gastric cell lines as well as S...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181556
更新日期:2019-01-08 00:00:00
abstract::A possible role for Ca2+ and calmodulin in the action of growth-hormone-releasing factor (GHRF) was investigated. Low extracellular Ca2+ (less than 100 microM), methoxyverapamil, flunarizine, cinnarizine, and Co2+ decreased both basal and GHRF-stimulated growth-hormone secretion, but did not totally inhibit GHRF-stimu...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116691
更新日期:1984-12-01 00:00:00
abstract::Background: Many epidemiologic studies have explored the association between age at last birth (ALB) and the risk of epithelial ovarian cancer, but the results remain controversial. Methods: A literature search was performed in PubMed, Web of Science, China National Knowledge Infrastructure (CNKI) and WanFang Med Onli...
journal_title:Bioscience reports
pub_type: 杂志文章,meta分析
doi:10.1042/BSR20182035
更新日期:2019-09-13 00:00:00
abstract::Permeant cationic fluorescent probes are widely employed to monitor mitochondrial transmembrane potential and its changes. The application of such potential-dependent probes in conjunction with both fluorescence microscopy and fluorescence spectroscopy allows the monitoring of mitochondrial membrane potential in indiv...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/s10540-007-9033-4
更新日期:2007-06-01 00:00:00
abstract::Circulating tumor cells can provide important diagnostic and prognostic information of the patients with non-small cell lung cancer (NSCLC). Aldehyde dehydrogenase 1 (ALDH1), a cancer stem cell marker, has been used in various tumors, including NSCLC. In the present study, we isolated the circulating ALDH1+ tumor cell...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180914
更新日期:2018-10-31 00:00:00
abstract::Hepatocellular carcinoma (HCC) has been one of the most highly lethal cancers. The acquisition of drug resistance accounts for the majority of poor effects of chemotherapy in HCC. Non-coding RNAs (ncRNAs) including miRNAs, long ncRNAs (lncRNAs), and circular RNA (circRNA) have been well-documented to participate in ca...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1042/BSR20180915
更新日期:2018-10-09 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116377
更新日期:1982-03-01 00:00:00
abstract::A range of different types of interactions at biological membrane surfaces have been studied using various different spin label electron spin resonance (ESR) techniques. These include: (1) the interfacial ionization of local anaesthetics, (2) the binding of peripheral membrane proteins, (3) the membrane insertion of t...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1023/a:1020590122846
更新日期:1999-08-01 00:00:00
abstract::Guanylate kinase is an essential and conserved enzyme in nucleotide biosynthetic pathway that transfers phosphoryl group of ATP to GMP for yielding GDP. Here, we report the phosphorylation of guanylate kinase from Mycobacterium tuberculosis (mGmk) by eukaryotic-type Ser/Thr kinase, PknA. Mass spectrometric studies ide...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20171048
更新日期:2017-11-15 00:00:00
abstract:BACKGROUND:Nasopharyngeal carcinoma (NPC) is a tumor that is commonly found in southern China. NPC has several risk factors, such as infection with the Epstein-Barr virus. However, we know little about the risk factors for eye metastasis (EM) in male patients with NPC. Serum lipids are well recognized as risk factors f...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20201082
更新日期:2020-06-26 00:00:00
abstract::Intracellular lipid-binding proteins (iLBPs) of the fatty acid-binding protein (FABP) family of animals transport, mainly fatty acids or retinoids, are confined to the cytosol and have highly similar 3D structures. In contrast, nematodes possess fatty acid-binding proteins (nemFABPs) that are secreted into the perivit...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20191292
更新日期:2019-07-23 00:00:00
abstract::In order to gain information on the metabolism of dolichol, the rates of excretion of dolichol and dolichyl phosphate were determined in rats maintained on a dolichol-free diet for 10 days. Analysis of fecal samples collected every 48 h yielded mean output values of 9.0 +/- 1.4 and 20.7 +/- 3.0 micrograms/rat/day of d...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01128818
更新日期:1984-09-01 00:00:00
abstract::eNOS (endothelial nitric oxide synthase) contains a MAPK (mitogen-activated protein kinase)-binding site associated with a major eNOS control element. Purified ERK (extracellular-signal-regulated kinase) phosphorylates eNOS with a stoichiometry of 2-3 phosphates per eNOS monomer. Phosphorylation decreases NO synthesis...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140015
更新日期:2014-09-17 00:00:00
abstract::Mice and rats are animals commonly used in research and laboratory testing. Compared with other animal species, they harbor many more zoonotic agents. Hymenolepis nana (H. nana) is a common tapeworm that parasitizes both humans and rodents. Although this tapeworm is of socio-economic importance worldwide, information ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181224
更新日期:2019-02-08 00:00:00
abstract::Gallbladder cancer (GBC) is a relatively rare but fatal gastrointestinal tumor. The microRNA-33b (miR-33b), a member of miR-33 family, is reported to function as a tumor suppressor in various cancers. Notably, miR-33 was predicted to target CROCC based on microarray-based analysis. Hereby, we aimed to characterize the...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190108
更新日期:2020-01-31 00:00:00