Abstract:
:Thrombospondins (TSPs) are evolutionarily-conserved, secreted glycoproteins that interact with cell surfaces and extracellular matrix (ECM) and have complex roles in cell interactions. Unlike the structural components of the ECM that form networks or fibrils, TSPs are deposited into ECM as arrays of nanoscale puncta. The cellular and molecular mechanisms for the patterning of TSPs in ECM are poorly understood. In the present study, we investigated whether the mechanisms of TSP patterning in cell-derived ECM involves actin cytoskeletal pathways or TSP oligomer state. From tests of a suite of pharmacological inhibitors of small GTPases, actomyosin-based contractility, or actin microfilament integrity and dynamics, cytochalasin D and jasplakinolide treatment of cells were identified to result in altered ECM patterning of a model TSP1 trimer. The strong effect of cytochalasin D indicated that mechanisms controlling puncta patterning depend on global F-actin dynamics. Similar spatial changes were obtained with endogenous TSPs after cytochalasin D treatment, implicating physiological relevance. Under matched experimental conditions with ectopically-expressed TSPs, the magnitude of the effect was markedly lower for pentameric TSP5 and Drosophila TSP, than for trimeric TSP1 or dimeric Ciona TSPA. To distinguish between the variables of protein sequence or oligomer state, we generated novel, chimeric pentamers of TSP1. These proteins accumulated within ECM at higher levels than TSP1 trimers, yet the effect of cytochalasin D on the spatial distribution of puncta was reduced. These findings introduce a novel concept that F-actin dynamics modulate the patterning of TSPs in ECM and that TSP oligomer state is a key determinant of this process.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Hellewell AL,Gong X,Schärich K,Christofidou ED,Adams JCdoi
10.1042/BSR20140168subject
Has Abstractpub_date
2015-05-20 00:00:00issue
3eissn
0144-8463issn
1573-4935pii
BSR20140168journal_volume
35pub_type
杂志文章abstract::Whether they are small enough to wriggle through the current-carrying part of an ionic channel or big enough to be kept outside and thus able to exert an osmotic stress on the channel space, polymers interact with channels in several instructive ways. The osmotic stress of excluded polymers allows one to measure the n...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01204353
更新日期:1995-12-01 00:00:00
abstract::The large size of a 1.5-MDa ice-binding adhesin [MpAFP (Marinomonas primoryensis antifreeze protein)] from an Antarctic Gram-negative bacterium, M. primoryensis, is mainly due to its highly repetitive RII (Region II). MpAFP_RII contains roughly 120 tandem copies of an identical 104-residue repeat. We have previously d...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140083
更新日期:2014-07-04 00:00:00
abstract::The existence of cancer stem cells (CSCs) is considered as a direct reason for the failure of clinic treatment in hepatocellular carcinoma (HCC). Growing evidences have demonstrated that miRNAs play an important role in regulation of stem cell proliferation, differentiation and self-renewal and their aberrances cause ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20150084
更新日期:2015-05-22 00:00:00
abstract::Safflower (Carthamus tinctorius L.) serves as a reference dicot for investigation of defence mechanisms in Asteraceae due to abundant secondary metabolites and high resistance/tolerance to environmental stresses. In plants, phenylpropanoid and flavonoid pathways are considered as two central defence signalling cascade...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140026
更新日期:2014-06-25 00:00:00
abstract::A few studies suggested the contribution of PPARs to the etiology of schizophrenia (SCZ). However, it is still not clear whether variants in PPAR-encoding genes have a direct association with SCZ. The potential linkage between SCZ and the variants within PPAR encoding genes (PPARA, PPARD, and PPARG) was tested in a la...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20201083
更新日期:2020-07-31 00:00:00
abstract::Glioblastomas (GBMs) are a lethal class of brain cancer, with a median survival <15 months in spite of therapeutic advances. The poor prognosis of GBM is largely attributed to acquired chemotherapy resistance, and new strategies are urgently needed to target resistant glioma cells. Here we report a role for miR-299-5p...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181051
更新日期:2018-09-12 00:00:00
abstract::Baicalin is the main bioactive component extracted from the traditional Chinese medicine Baical Skullcap Root, and its anti-tumor activity has been studied in previous studies. PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK), a serine/threonine protein kinase, is highly expressed in many cancer cell...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181692
更新日期:2019-04-09 00:00:00
abstract::Regulator of G protein signaling (RGS) proteins are GTPase-activating proteins (GAP) for G protein alpha-subunits and are thought to be responsible for rapid deactivation of G protein mediated signaling pathway. In this present study, we demonstrate that PA is the most efficient candidate to inhibit GAP activity of RG...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1023/b:bire.0000007694.71158.02
更新日期:2003-08-01 00:00:00
abstract::Purpose: Wilson's disease (WD) is a genetic disorder of copper metabolism with pathological copper accumulation in the brain. The purpose of the present study was to evaluate the relationship between the damaged white matter and the impaired cognitive function in WD patients. Materials and methods: Thirty WD adolescen...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181651
更新日期:2019-03-15 00:00:00
abstract::Acquired resistance of afatinib is a significant challenge for non-small cell lung cancer (NSCLC) therapy and the mechanisms remain unclear. Aberrant activation of epidermal growth factor receptor (EGFR)-dependent downstream pathways, especially phosphatidylinositol-3-kinases/protein kinase B (PI3K/Akt) signaling path...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181693
更新日期:2018-12-11 00:00:00
abstract::Canstatin, the non-collagenous domain of collagen type IV α-chains, belongs to a series of collagen-derived angiogenic inhibitors. In this study, the inhibitory effect of recombinant canstatin on tumour growth was investigated using a gastric cancer xenograft model. The volume and weight of tumours in mice treated wit...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140012
更新日期:2014-04-01 00:00:00
abstract::Intervertebral disc degeneration is a disease identified as an inflammation response-participated pathological process. As a classical cellular feature, disc cell senescence is reported to be closely related with disc cell senescence. Resveratrol has a protective role against inflammation in some cells. However, its b...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190126
更新日期:2019-05-10 00:00:00
abstract::Emerging evidence are accumulating that long noncoding RNAs (lncRNAs) have recently been identified to participate in various cellular processes. Terminal differentiation induced ncRNA (TINCR) is a newly identified lncRNA with its functional roles not fully elucidated in human malignancy. The current study aims to ide...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170301
更新日期:2017-07-27 00:00:00
abstract::The family of 5-HT4 receptors comprises 16 putative splice variants. We have previously shown that there are differences in signal transduction of the h5-HT(4a) and h5-HT(4b) receptors. In the present study, the internalization of these two splice variants following receptor stimulation was investigated with confocal ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/s10540-005-2582-5
更新日期:2004-06-01 00:00:00
abstract::Background: Previous studies have found that hydrogen sulfide (H2S) has multiple functions such as anti-inflammatory, antioxidative in addition to biological effects among the various organs. Exaggerated proliferation and resistance to apoptosis of pulmonary artery smooth muscle cells (PASMCs) is a key component of va...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190304
更新日期:2019-07-08 00:00:00
abstract::Aspergillus fumigatus is the causative agent of IA (invasive aspergillosis) in immunocompromised patients. It possesses a cell wall composed of chitin, glucan and galactomannan, polymeric carbohydrates synthesized by processive glycosyltransferases from intracellular sugar nucleotide donors. Here we demonstrate that A...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20130053
更新日期:2013-09-04 00:00:00
abstract::Polycationic amino acids induce the leakage and fusion of liposomes containing anionic lipids. We have investigated the nature and extent of the changes in membrane physical properties caused by these polypeptides which could result in the observed membrane destabilization. We found that in the range of pH 5 to pH 7 b...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01207455
更新日期:1995-06-01 00:00:00
abstract::The high proliferation rate of tumor cells demands high energy and metabolites that are sustained by a high glycolytic flux known as the 'Warburg effect'. The activation and further metabolism of glucose is initiated by hexokinase, a focal point of metabolic regulation. The human hexokinase 2 (HK2) is overexpressed in...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20171666
更新日期:2018-02-21 00:00:00
abstract::Muscle tissue levels of d1-alpha-tocopherol (vitamin E) were significantly lower in endurance-trained rats than in sedentary animals, whether the animals were fed on vitamin-E-deficient or control (vitamin-E-sufficient) diets. In vitamin-E-deficient rats, liver tissue levels of vitamin E were significantly lower in th...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119661
更新日期:1984-03-01 00:00:00
abstract::In recent years, the antimicrobial activity of peptides isolated from a wide variety of organs from plant species has been reported. However, a few studies have investigated the potential of antimicrobial peptides (AMPs) found in fruits, especially Capsicum chinense (pepper). The present study aimed to purify and char...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200950
更新日期:2020-08-28 00:00:00
abstract::A riboflavin carrier protein has been purified from human pregnancy and umbilical cord sera by affinity chromatography and fast protein liquid chromatography. This protein has a similar molecular weight to the chicken egg riboflavin carrier protein and shares other physicochemical properties, such as pI and riboflavin...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119773
更新日期:1987-07-01 00:00:00
abstract::To investigate whether the human anti-thrombospondin type 1 domain-containing 7A (THSD7A) antibody-induced membranous nephropathy (MN) is mediated by activating lectin complement pathway. Automatic biochemical apparatus was used to assess renal function of mice. The serum levels of anti-THSD7A antibodies and complemen...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180131
更新日期:2018-06-21 00:00:00
abstract::Tumor microenvironment (TME) is the cellular environment in which tumor exists, and it contributes to tumor formation and progression. The TME is composed of tumor cells, stromal cells, cytokines, and chemotactic factors of which fibroblasts are the main cellular components. In our present study, we found that colorec...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180243
更新日期:2018-12-07 00:00:00
abstract::Osteoarthritis (OA) is one of the most common chronic joint disease. Long non-coding RNAs (lncRNAs) have been confirmed to play important roles in a variety of diseases including OA. However, the underlying mechanism of lncRNA differentiation antagonizing non-protein coding RNA (DANCR) in OA has not been well elucidat...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181228
更新日期:2018-12-14 00:00:00
abstract::Cryogenic transmission electron microscopy (cryo-TEM) is a high-resolution biological imaging method, whereby biological samples, such as purified proteins, macromolecular complexes, viral particles, organelles and cells, are embedded in vitreous ice preserving their native structures. Due to sensitivity of biological...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170203
更新日期:2018-04-27 00:00:00
abstract::Bifunctional dihaem cytochrome c thiosulfate dehydrogenases/tetrathionate reductases (TsdA) exhibit different catalytic properties depending on the source organism. In the human food-borne intestinal pathogen Campylobacter jejuni, TsdA functions as a tetrathionate reductase enabling respiration with tetrathionate as a...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160457
更新日期:2016-12-09 00:00:00
abstract::MicroRNAs (miRNAs) have been frequently identified as key mediators in almost all developmental and pathological processes, including those in the liver. The present study was conducted with aims of investigating the role of microRNA-138 (miR-138) in acute liver failure (ALF) via a mechanism involving p53 and liver ma...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190763
更新日期:2019-07-18 00:00:00
abstract::Recent experimental evidence increasingly shows that the dysregulation of cellular bioenergetics is associated with a wide array of common human diseases, including cancer, neurological diseases and diabetes. Respiration provides a vital source of cellular energy for most eukaryotic cells, particularly high energy dem...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160083
更新日期:2016-06-30 00:00:00
abstract::Choroideremia is a complex form of blindness-causing retinal degeneration. The aim of the present study was to investigate the pathogenic variant and molecular etiology associated with choroideremia in a Chinese family. All available family members underwent detailed ophthalmological examinations. Whole exome sequenci...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200067
更新日期:2020-05-29 00:00:00
abstract::Ehrlich Ascites Tumour cells were irradiated with UV-light to crosslink DNA to proteins in vivo. The DNA fragments associated with the nuclear lamina were purified and characterized. The results of the Cot analysis and the hybridization experiments suggest that the DNA fragments attached to the nuclear lamina although...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119802
更新日期:1989-10-01 00:00:00