Abstract:
:The existence of cancer stem cells (CSCs) is considered as a direct reason for the failure of clinic treatment in hepatocellular carcinoma (HCC). Growing evidences have demonstrated that miRNAs play an important role in regulation of stem cell proliferation, differentiation and self-renewal and their aberrances cause the formation of CSCs and eventually result in carcinogenesis. We recently identified miRNA-148b as one of the miRNAs specifically down-regulated in side population (SP) cells of PLC/PRF/5 cell line. However, it remains elusive how miRNA-148b regulates CSC properties in HCC. In the present study, we observed that overexpression or knockdown of miR-148b through lentiviral transfection could affect the proportion of SP cells as well as CSC-related gene expression in HCC cell lines. In addition, miR-148b blocking could stimulate cell proliferation, enhance chemosensitivity, as well as increase cell metastasis and angiogenesis in vitro. More importantly, miR-148b could significantly suppress tumorigenicity in vivo. Further studies revealed that Neuropilin-1 (NRP1), a transmembrane co-receptor involved in tumour initiation, metastasis and angiogenesis, might be the direct target of miRNA-148b. Taking together, our findings define that miR-148b might play a critical role in maintenance of SP cells with CSC properties by targeting NRP1 in HCC. It is the potential to develop a new strategy specifically targeting hepatic CSCs (HCSCs) through restoration of miR-148b expression in future therapy.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Liu Q,Xu Y,Wei S,Gao W,Chen L,Zhou T,Wang Z,Ying M,Zheng Qdoi
10.1042/BSR20150084subject
Has Abstractpub_date
2015-05-22 00:00:00issue
4eissn
0144-8463issn
1573-4935pii
BSR20150084journal_volume
35pub_type
杂志文章abstract::The 250-kDa sialoglycoprotein of bovine erythrocyte membranes, GP-2, has been found to be an exceptionally rich source of branched sialo-oligosaccharides of poly-N-acetyllactosamine (I antigen) type with receptor activity for the human pathogen Mycoplasma pneumoniae. Desialylated GP-2 is the most potent I-active subst...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01128815
更新日期:1984-09-01 00:00:00
abstract::Transfer of Bomirski amelanotic melanoma cells from in vivo to in vitro growth conditions results in occurrence of rapid melanization in their cytoplasm. The melanized cells from primary cell culture initiate tumours in hamsters, which do not contain traces of melanin and resemble typical amelanotic melanoma. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121951
更新日期:1983-02-01 00:00:00
abstract::Antimicrobial peptides are a promising resource for developing novel antibiotic and even anticancer drugs. Here, a 28-mer polypeptide, Ranatuerin-2PLx (R2PLx), was identified from lyophilised skin secretions. The chemically synthetic replicates exhibited moderate and broadspectrum antimicrobial effect against various ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180710
更新日期:2018-11-09 00:00:00
abstract::Triacylglycerol/fatty acid substrate cycling was measured in vivo in brown adipose tissue (BAT) and white adipose tissue (WAT) of fed, starved and refed rats. Starvation (24h) significantly decreased the rate of cycling in BAT, and refeeding chow diet led to a rapid, 6-fold increase in cycling. Cycling rate in WAT was...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116459
更新日期:1988-04-01 00:00:00
abstract:OBJECTIVE:The role of lncRNAs in tumor has been widely concerned. The present study took HAS2-AS1 (the antisense RNA 1 of HAS2) as a starting point to explore its expression in glioma and its role in the process of migration and invasion, providing a strong theoretical basis for mining potential therapeutic targets of ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200487
更新日期:2020-08-28 00:00:00
abstract::The role of the topoisomerase enzyme in DNA recombination was investigated by extracting chromosomal deoxyribonucleoproteins from a variety of cultured mammalian cells and assaying for the formation of recombinant DNA structures. Although each of the crude deoxyribonucleoprotein preparations contained topoisomerase ac...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01115252
更新日期:1982-07-01 00:00:00
abstract::Incubation of mouse thymocytes with 10 microM monensin for 1 hour induces morphological alterations characterized by the extensive dilatation and vacuolization of the Golgi complex. This effect is used to study the transport and utilization of labelled sugar nucleotides into intracellular vesicles by using thymocytes ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01115011
更新日期:1986-02-01 00:00:00
abstract::Imipramine and clorgyline, at concentrations of 0.002 M, inhibit the respiration of brain tissue by 82 and 71 per cent respectively, while chloropromazine and tranylcypromine, at concentrations of 0.01 M, inhibit it about 25 per cent. Deprenyl and amphetamine at a concentration of 0.002 M inhibit brain tissue respirat...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01118600
更新日期:1991-02-01 00:00:00
abstract::Decreasing extracellular sodium concentration was found to produce a contractile response of rabbit ileal smooth muscle. As the concentration decreases, the amplitude of contraction increases, thus producing a dose-dependent curve. Harmaline, a competitor for sodium, was found to inhibit the sodium gradient-dependent ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116998
更新日期:1985-08-01 00:00:00
abstract::A slow phase of linear exocytosis by fibroblasts of previously endocytosed fluid, succeeding the initial burst of release, is described. This is not due to cell lysis, is virtually abolished at 4 degrees C, and is retarded when the lysosomal system is expanded. It may reflect membrane recycling. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01314215
更新日期:1982-08-01 00:00:00
abstract:OBJECTIVE:The current study aimed to investigate the functional roles and clinical significance of microRNA-148a (miR-148a) in the progression of oral squamous cell carcinoma (OSCC). METHODS:Relative expression of miR-148a in OSCC cells and tissues were detected using quantitative real-time polymerase chain reaction (...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20182458
更新日期:2020-04-30 00:00:00
abstract:OBJECTIVE:Gastric cancer is the most common gastrointestinal malignancy in China and results from a combination of genetic and environmental factors. The present study was conducted to investigate the relationship between long noncoding RNA (lncRNA) materally expressed gene 3 (MEG3) single nucleotide polymorphisms (SNP...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200305
更新日期:2020-11-27 00:00:00
abstract::Oral vaccination requires an antigen delivery vehicle to protect the antigen and to enhance translocation of the antigen to the mucosa-associated lymphoid tissue. A variety of antigen delivery vehicles including liposomes have been studied for mucosal immunization. The advantages of liposome formulations are their par...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1023/a:1020103109483
更新日期:2002-04-01 00:00:00
abstract::Understanding cell migration in a 3D microenvironment is essential as most cells encounter complex 3D extracellular matrix (ECM) in vivo Although interactions between cells and ECM have been studied previously on 2D surfaces, cell migration studies in 3D environment are still limited. To investigate cell migration und...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181596
更新日期:2019-02-19 00:00:00
abstract::Sulphate efflux from human placental microvillus membrane vesicles was inhibited by external DIDS (KI congruent to 10(-6) M). This inhibition was partially reversed on addition of the translocated substrates sulphate or selenate to the external medium: selenite which is not translocated does not protect against DIDS i...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116242
更新日期:1986-10-01 00:00:00
abstract::Acquired resistance of afatinib is a significant challenge for non-small cell lung cancer (NSCLC) therapy and the mechanisms remain unclear. Aberrant activation of epidermal growth factor receptor (EGFR)-dependent downstream pathways, especially phosphatidylinositol-3-kinases/protein kinase B (PI3K/Akt) signaling path...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181693
更新日期:2018-12-11 00:00:00
abstract::The sarcolemma of smooth-muscle cells from human pregnant myometrium possesses a high-affinity Ca2+-ATPase, which has the characteristics of an active Ca2+-extrusion pump. This pump enzyme, either membrane-bound or solubilized, was strongly inhibited by oxytocin (half-maximal inhibition at about 4 microU/ml or 10 pM)....
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117437
更新日期:1985-01-01 00:00:00
abstract::MicroRNAs (miRNAs) have been frequently identified as key mediators in almost all developmental and pathological processes, including those in the liver. The present study was conducted with aims of investigating the role of microRNA-138 (miR-138) in acute liver failure (ALF) via a mechanism involving p53 and liver ma...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190763
更新日期:2019-07-18 00:00:00
abstract::Common colds were shortened by 7 days in a 1984 clinical trial using zinc gluconate throat lozenges each 2 h. Between then and 2004, 10 other double-blind, placebo-controlled clinical trials showed widely varying results. This re-analysis of these trials presents solution chemistry methods to elucidate differences in ...
journal_title:Bioscience reports
pub_type: 杂志文章,meta分析
doi:10.1023/b:bire.0000037754.71063.41
更新日期:2004-02-01 00:00:00
abstract::The extracts of Paulownia tomentosa (P. tomentosa) exhibit multiple pharmacological activities. In the present study, P. tomentosa flower polysaccharides (PTFP) were extracted by water decoction and ethanol precipitation, and the immunologic modulations of PTFP against Newcastle disease (ND) vaccine was investigated i...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190224
更新日期:2019-05-03 00:00:00
abstract::Baicalin is the main bioactive component extracted from the traditional Chinese medicine Baical Skullcap Root, and its anti-tumor activity has been studied in previous studies. PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK), a serine/threonine protein kinase, is highly expressed in many cancer cell...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181692
更新日期:2019-04-09 00:00:00
abstract::Facioscapulohumeral muscular dystrophy (FSHD) region gene 1 (FRG1) is a candidate gene for FSHD. FRG1 regulates various muscle-related functions, but studies have proposed its role in development and angiogenesis also, where it is involved with tumor-associated molecules. Therefore, we decided to look into its role in...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20171062
更新日期:2017-10-27 00:00:00
abstract::Background: Single-nucleotide polymorphisms (SNPs) in lncRNAs could be biomarkers for susceptibility to colorectal cancer (CRC), but the association of PCAT1 polymorphisms and CRC susceptibility is yet to be studied. Methods: Five tagSNPs covering the PCAT1 gene were detected through Kompetitive Allele-Specific PCR am...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190708
更新日期:2019-07-12 00:00:00
abstract::Cilia and flagella are critical organelles with conserved internal structures and diverse developmental and physiological processes according to cell type. Although the core components of structures are shared with thousands of associated proteins involved in cilia or flagella formation, we hypothesized that some unkn...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20192666
更新日期:2020-01-31 00:00:00
abstract::PDC (pyruvate dehydrogenase complex) is a multi-enzyme complex comprising an E1 (pyruvate decarboxylase), an E2 (dihydrolipomide acetyltransferase) and an E3 (dihydrolipoamide dehydrogenase). PDC catalyses the decarboxylation of pyruvate and forms acetyl-CoA and NADH. In the human malaria parasite Plasmodium falciparu...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140150
更新日期:2015-01-14 00:00:00
abstract::An ontogenetic study of the effect of various neurohormones and other activators on adenylate cyclase systems was carried out using cultures of cells from 15-d-old embryonic mouse brain. Dopamine stimulated the enzyme activity at earlier culture ages (i.e. 4 and 10 d) but had little stimulatory effect at later ages (i...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117073
更新日期:1985-07-01 00:00:00
abstract::Thrombospondin-2 (THBS2) is a secreted protein overexpressed in numerous cancers and may function as a diagnostic tumor marker. The objective of the present study was to investigate the diagnostic performance of serum THBS2 in early stage non-small-cell lung cancer (NSCLC). Serum THBS2 and Cyfra21-1 level were evaluat...
journal_title:Bioscience reports
pub_type: 临床试验,杂志文章
doi:10.1042/BSR20190476
更新日期:2019-07-25 00:00:00
abstract::The large size of a 1.5-MDa ice-binding adhesin [MpAFP (Marinomonas primoryensis antifreeze protein)] from an Antarctic Gram-negative bacterium, M. primoryensis, is mainly due to its highly repetitive RII (Region II). MpAFP_RII contains roughly 120 tandem copies of an identical 104-residue repeat. We have previously d...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140083
更新日期:2014-07-04 00:00:00
abstract::Retinitis pigmentosa (RP) is a group of hereditary, degenerative retinal disorders characterized by progressive retinal dysfunction, outer retina cell loss, and retinal tissue atrophy. It eventually leads to tunnel vision and legal, or total blindness. Here we aimed to reveal the causal gene and mutation contributing ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20171300
更新日期:2018-04-27 00:00:00
abstract::Kinesin family member 14 (KIF14) is a member of kinesin family proteins which have been found to be dysregulated in various cancer types. However, the expression of KIF14 and its potential prognostic significance have not been investigated in cervical cancer. Real-time PCR was performed to assess the expression levels...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20150314
更新日期:2016-04-05 00:00:00