Localized energy coupling during photophosphorylation by chromatophores of Rhodopseudomonas capsulata N22.

Abstract:

:The principle of the dual inhibitor titration method for testing models of electron-transport phosphorylation is outlined, and the method is applied to the study of photophosphorylation in bacterial chromatophores. It is concluded that energy coupling is strictly localized in nature in this system, in the sense that free energy released by a particular electron-transport chain may be used only by a particular H+-ATP synthase. Dual inhibitor titrations using the uncoupler SF 6847 and the H+-ATP synthase inhibitor oligomycin indicate that uncouplers act by shuttling rapidly between the localized energy-coupling sites.

journal_name

Biosci Rep

journal_title

Bioscience reports

authors

Hitchens GD,Kell DB

doi

10.1007/BF01114933

subject

Has Abstract

pub_date

1982-10-01 00:00:00

pages

743-9

issue

10

eissn

0144-8463

issn

1573-4935

journal_volume

2

pub_type

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