Abstract:
:Resistance to chemotherapy is one of main obstacles in the treatment of colorectal cancer (CRC). However, the mechanisms are still unclear, and the treatment options are still limited. miR-543 has been indicated to act as an oncogene in some cancers, but its function in regulating chemoresistance has not been considered in CRC cells. This study investigated whether the down-regulation of miR-543 expression enhanced 5-fluorouracil (5-FU)-induced apoptosis in HCT8/FU colon cancer cells. In our study, qRT-PCR revealed that miR-543 expression was up-regulated in the HCT8/FU colon cancer cell line compared with that of HCT8 colon cancer cell line. An miR-543 inhibitor or mimic was transfected, followed by MTT assay to detect 5-FU sensitivity in HCT8 and HCT8/FU cell lines, which showed that IC50 of 5-FU was positively correlated with miR-543 expression. Further studies showed that miR-543 enhanced drug resistance by down-regulating the expression of phosphatase and tensin homolog (PTEN), which negatively regulates protein kinase B (AKT) activation. Additionally, an elevated expression of PTEN reversed the chemoresistance of miR-543-overexpressing HCT8 cells to 5-FU. These results indicate that miR-543 might be a target to increase the sensitivity of CRC cells to 5-FU through the PTEN/PI3K/AKT pathway.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Liu G,Zhou J,Dong Mdoi
10.1042/BSR20190249subject
Has Abstractpub_date
2019-03-22 00:00:00issue
3eissn
0144-8463issn
1573-4935pii
BSR20190249journal_volume
39pub_type
杂志文章abstract::Background: Selenoprotein S (SelS) gene expression is positively correlated to triglyceride (TG) concentrations and is associated with diabetes in animal model. However, the relationship between genetic polymorphisms of SelS and Type 2 diabetes (T2DM) remains unclear. Methods: In the present study, we genotyped four s...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181696
更新日期:2018-11-28 00:00:00
abstract::Osteoarthritis (OA) is one of the most common chronic joint disease. Long non-coding RNAs (lncRNAs) have been confirmed to play important roles in a variety of diseases including OA. However, the underlying mechanism of lncRNA differentiation antagonizing non-protein coding RNA (DANCR) in OA has not been well elucidat...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181228
更新日期:2018-12-14 00:00:00
abstract:OBJECTIVE:Gastric cancer is the most common gastrointestinal malignancy in China and results from a combination of genetic and environmental factors. The present study was conducted to investigate the relationship between long noncoding RNA (lncRNA) materally expressed gene 3 (MEG3) single nucleotide polymorphisms (SNP...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200305
更新日期:2020-11-27 00:00:00
abstract::Peanut (Arachis hypogaea) seed lectin, PNA is widely used to identify tumor specific antigen (T-antigen), Galbeta1-3GalNAc on the eukaryotic cell surface. The functional amino acid coding region of a cDNA clone, pBSH-PN was PCR amplified and cloned downstream of the polyhedrin promoter in the Autographa californica nu...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1023/a:1020234005099
更新日期:1999-06-01 00:00:00
abstract::Mitochondrial disorders encompass any medical specialty and affect patients at any age. Likewise, the spectrum of clinical and genetic signatures of these disorders is ample, making a precise diagnosis difficult. We will report some of the major clinical phenotypes observed in infancy, their underlining molecular feat...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/s10540-007-9039-y
更新日期:2007-06-01 00:00:00
abstract::In order to better understand the role of cell surface glycolipids in T lymphocyte activation, heparin was used to simultaneously modulate the expression of glycolipids and the lytic capacity of lymphocytes activated by interleukin-2. Results presented here show that heparin added at the start of a 3 day culture inhib...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01115230
更新日期:1988-08-01 00:00:00
abstract::Cervical cancer (CC) is ranked as the fourth most common cancer that occurs in women universally, which normally causes pain in the lower belly. Plenty of studies have stated that the expression of long non-coding RNAs (lncRNAs) is linked to the cellular development of many kinds of cancers. DSCAM-AS1 has been reporte...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20192061
更新日期:2020-01-31 00:00:00
abstract::Blood platelets have the capacity to participate in a number of physiological as well as pathological processes within the circulation. In order to evaluate their cellular reactivity a number of platelet function tests have been developed. The main in vitro function tests are assessment of aggregation and adhesion, se...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114683
更新日期:1989-06-01 00:00:00
abstract::Erythrocyte membrane is crucial to maintain the stability of erythrocyte structure. The membrane protein on the surface of erythrocyte membrane enables erythrocyte to have plasticity and pass through the microcirculation without being blocked or destroyed. Decreased deformability of erythrocyte membrane protein will l...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200799
更新日期:2020-06-26 00:00:00
abstract::This article discusses how changes in luminal calcium concentration affect calcium release rates from triad-enriched sarcoplasmic reticulum vesicles, as well as single channel opening probability of the ryanodine receptor/calcium release channels incorporated in bilayers. The possible participation of calsequestrin, o...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/BF01788370
更新日期:1995-10-01 00:00:00
abstract::A key mechanism mediating cellular adaptive responses to hypoxia involves the activity of hypoxia-inducible factor 1 (HIF-1), a transcription factor composed of HIF-1α, and HIF-1β subunits. The classical mechanism of regulation of HIF-1 activity involves destabilisation of HIF-1α via oxygen-dependent hydroxylation of ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20192345
更新日期:2020-01-31 00:00:00
abstract::Bleaching of the purple membrane strongly reduces the number of divalent cation binding sites as well as their affinities. Conversely, deionization of the bleached membrane drastically inhibits the chromophore regeneration. Proteolysis experiments using bromelain show that the bleached membrane has an additional cleav...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114972
更新日期:1986-11-01 00:00:00
abstract::Chemical transformations, like osmotic translocations, are transport processes when looked at in detail. In chemiosmotic systems, the pathways of specific ligand conduction are spatially orientated through osmoenzymes and porters in which the actions of chemical group, electron and solute transfer occur as vectorial (...
journal_title:Bioscience reports
pub_type:
doi:10.1007/s10540-005-2739-2
更新日期:2004-08-01 00:00:00
abstract::Brain cells dissociated from 15-day-old embryonic mice and grown in culture contain both cytosolic and nuclear receptors for L-3,5,3'-triiodothyronine (L-T3). KD values for L-T3 of 3.05 X 10(-9) M and 4.2 X 10(-9) M were determined with the cytosolic and nuclear receptors respectively. These cultured cells, which are ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114868
更新日期:1981-04-01 00:00:00
abstract::Three myoid-cell clones were established from the thymuses of two Wistar rats; one thymus yielded two clones, R615A and R615B2, and the other yielded one clone, R613Ad. The three clones were divided into two subtypes. Both subtypes were able to form myofibrils, expressed AChR on their cell-surface membrane, and contai...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116890
更新日期:1984-11-01 00:00:00
abstract::Cathepsin D occurs in two forms, a single polypeptide chain (Mr 44 000) and a non-covalent complex of two peptides of Mr 14 000 and 30 000 that is derived by proteolytic processing of the 44 000 polypeptide. The two forms from bovine spleen are closely similar in secondary structure content, in aromatic amino acid env...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119908
更新日期:1985-10-01 00:00:00
abstract::The nucleotide sequence of the thioredoxin gene from Escherichia coli was determined. The structural gene was identified on a cloned 3-kb PvuII fragment by hybridization with a synthetic oligodeoxyribonucleotide corresponding to a part of the amino acid sequence of thioredoxin. Restriction-enzyme fragments were used a...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116889
更新日期:1984-11-01 00:00:00
abstract::Although wasting marmoset syndrome (WMS) is one of the biggest problems facing captive marmoset colonies, the mechanisms underlying its pathogenesis remain unclear. In our clinical experience, it is difficult to cure WMS-affected marmosets with severe hypoalbuminemia. Thus, the mechanisms underlying hypoalbuminemia in...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190562
更新日期:2019-07-08 00:00:00
abstract::CXCL8, a member of CXC chemokines, was constitutively expressed in many types of human cancers, and its overexpression has been shown to play a critical role in promoting tumorigenesis. The purpose of the present study was to determine CXCL8 expression in a commercial human liver tissue microarray, and elucidate the e...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20201169
更新日期:2020-08-28 00:00:00
abstract::TRα1 (thyroid hormone receptor α1) is well recognized for its importance in brain development. However, due to the difficulties in predicting TREs (thyroid hormone response elements) in silico and the lack of suitable antibodies against TRα1 for ChIP (chromatin immunoprecipitation), only a few direct TRα1 target genes...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20120124
更新日期:2013-03-13 00:00:00
abstract::The discovery of genes that control cell division in yeast, and their relation to cancer, is reviewed. ...
journal_title:Bioscience reports
pub_type: 传,历史文章
doi:10.1023/a:1020918107706
更新日期:2002-06-01 00:00:00
abstract::Transfer of Bomirski amelanotic melanoma cells from in vivo to in vitro growth conditions results in occurrence of rapid melanization in their cytoplasm. The melanized cells from primary cell culture initiate tumours in hamsters, which do not contain traces of melanin and resemble typical amelanotic melanoma. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121951
更新日期:1983-02-01 00:00:00
abstract::We investigated the possible translational role which elevated concentrations of highly purified Semliki Forest virus (SFV) capsid (C)-protein molecules may play in a cell-free translation system. Here we demonstrate that in the absence of double-stranded RNA high concentrations of C protein triggered the phosphorylat...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01198464
更新日期:1996-12-01 00:00:00
abstract::Long non-coding RNA (lncRNA) H19 has been implicated in tumor angiogenesis. However, whether H19 regulates the progression of corneal neovascularization (CNV) is unclear. The present study aimed to determine the function of H19 in CNV and its possible molecular mechanism. Here, we found that the H19 levels were remark...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20182394
更新日期:2019-05-02 00:00:00
abstract::Nonsmall cell lung cancer (NSCLC) is one of the most frequent malignancies worldwide. Long noncoding RNAs (LncRNAs) play critical roles in cancer initiation and progression. Previous studies have demonstrated that overexpression of cytoskeleton regulator RNA (CYTOR) predicates poor prognosis and promotes tumor progres...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181599
更新日期:2018-12-18 00:00:00
abstract::Hypocaloric diet feeding reduced the mitochondrial protein content and whole tissue GDP-binding in interscapular brown adipose tissue from both virgin and lactating rats. A reduction in brown fat lipoprotein lipase activity was also detected in underfed virgin and lactating animals. These results indicate that lactati...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114762
更新日期:1986-07-01 00:00:00
abstract::A natural avermectin complex, aversectin C, was shown to be capable of exerting selective cytostatic and neurotoxic effects on mammalian cells. Specifically, it killed proliferating neuroblastoma B103 cells but was non-toxic for differentiated cells of this culture. The antiproliferation action of aversectin C was not...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1023/a:1020262811459
更新日期:1999-12-01 00:00:00
abstract::Wilms tumor is the most common pediatric malignancy in the kidney. The miR34b/c is a downstream target gene of the transcription factor p53. The important role of TP53 mutations, the methylation of miR34b/c, and the interaction between these two molecules in tumorigenesis have been well documented. Due to the biologic...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20194202
更新日期:2020-02-28 00:00:00
abstract::The molecular mechanism of the occurrence and development of papillary thyroid carcinoma (PTC) has been widely explored, but has not been completely elucidated. The present study aimed to identify and analyze genes associated with PTC by bioinformatics methods. Two independent datasets were downloaded from Gene Expres...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190083
更新日期:2019-04-02 00:00:00
abstract::Cell membranes have important functions in many steps of the blood coagulation cascade, including the activation of factor X (FX) by the factor VIIa (FVIIa)-tissue factor (TF) complex (extrinsic Xase). FVIIa shares structural similarity with factor IXa (FIXa) and FXa. FIXa and FXa are regulated by binding to phosphati...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20204077
更新日期:2021-01-22 00:00:00