Abstract:
:In order to better understand the role of cell surface glycolipids in T lymphocyte activation, heparin was used to simultaneously modulate the expression of glycolipids and the lytic capacity of lymphocytes activated by interleukin-2. Results presented here show that heparin added at the start of a 3 day culture inhibited the formation of lymphokine activated killer cells by up to 50%. Heparin also has a profound effect on the synthesis of glycolipids during this three day period. Asialo GM1, a useful cell surface marker for subsets of murine cytotoxic cells, is reduced in amount, as are the other two major neutral glycolipids lactosylceramide and asialo GM2. In addition, the synthesis of some gangliosides is affected by heparin treatment. Comparison of the glycosyltransferase activities of untreated and heparin-treated cells shows that the activities of a 2-3-sialyltransferase and a beta 1-3 galactosyltransferase are inhibited dramatically, while a third enzyme, N-acetyl-galactosaminyltransferase is unaffected. The two heparin inhibitable enzymes bind to heparin affinity columns but the galactosaminyltransferase does not. These studies suggest that the proper regulation of the activities of specific glycosyltransferases may be important events in lymphocyte activation.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Schwarting GA,Gajewski Adoi
10.1007/BF01115230subject
Has Abstractpub_date
1988-08-01 00:00:00pages
389-99issue
4eissn
0144-8463issn
1573-4935journal_volume
8pub_type
杂志文章abstract::Maclurin, the potent non-specific blood-group hemagglutinin present in extracts of Maclura pomifera, has been purified by a new biospecific affinity-chromatographic procedure. Additional studies have indicated that this hemagglutinin occurs as five closely related tetrameric protein isoforms derived from two non-coval...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01136724
更新日期:1982-04-01 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1042/BSR20190377
更新日期:2019-08-02 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181090
更新日期:2018-11-15 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181176
更新日期:2018-10-09 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190091
更新日期:2019-04-16 00:00:00
abstract:BACKGROUND:Disc degeneration is correlated with mechanical load. Osteogenic protein-1 (OP-1) is potential to regenerate degenerative disc. OBJECTIVE:To investigate whether OP-1 can protect against high magitude compression-induced nucleus pulposus (NP) cell apoptosis and NP matrix catabolism, and its potential mechani...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180018
更新日期:2018-02-13 00:00:00
abstract::We have investigated the intramembranal ion traffic in apoptotic 3T6 cells in culture. Apoptosis was induced by various treatments, such as serum deprivation, high density growth and hydrogen peroxide at subnecrotic doses. Cell death was assessed by nucleosomal DNA fragmentation, single cell electrophoresis, immunoflu...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1023/a:1027364308147
更新日期:1997-12-01 00:00:00
abstract::Long non-coding RNA (lncRNA) H19 has been implicated in tumor angiogenesis. However, whether H19 regulates the progression of corneal neovascularization (CNV) is unclear. The present study aimed to determine the function of H19 in CNV and its possible molecular mechanism. Here, we found that the H19 levels were remark...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20182394
更新日期:2019-05-02 00:00:00
abstract::Mitochondria are known to participate in the initiation of programmed cell death (PCD) in animals and in plants. The role of chloroplasts in PCD is still unknown. We describe a new system to study PCD in plants; namely, leaf epidermal peels. The peel represents a monolayer consisting of cells of two types: phototrophi...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1023/a:1025576307912
更新日期:2003-04-01 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180367
更新日期:2018-05-22 00:00:00
abstract::The solvation properties of ubiquinone-10 and ubiquinol-10 in a wide variety of solvents of polarity varying from alkanes to water are reported. Greatest solubility is observed in solvents of intermediate polarity and particularly where low polarity is combined with a pronounced tendency to interact with the benzoquin...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117101
更新日期:1986-09-01 00:00:00
abstract::Wilms tumor is the most common pediatric malignancy in the kidney. The miR34b/c is a downstream target gene of the transcription factor p53. The important role of TP53 mutations, the methylation of miR34b/c, and the interaction between these two molecules in tumorigenesis have been well documented. Due to the biologic...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20194202
更新日期:2020-02-28 00:00:00
abstract::Calmodulin-free ghost membranes were prepared from erythrocytes of kwashiorkor children and from healthy children in the same age bracket. In the absence of calmodulin, the specific activity of Mg2+-dependent Ca2+-pumping ATPase (Ca2+ + Mg2+-ATPase) of kwashiorkor membranes was more than 40 percent lower than the spec...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114689
更新日期:1989-06-01 00:00:00
abstract::Transfer of Bomirski amelanotic melanoma cells from in vivo to in vitro growth conditions results in occurrence of rapid melanization in their cytoplasm. The melanized cells from primary cell culture initiate tumours in hamsters, which do not contain traces of melanin and resemble typical amelanotic melanoma. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121951
更新日期:1983-02-01 00:00:00
abstract::Signal transducer and activator of transcription (STAT), a family of latent cytoplasmic transcription factors, are composed of seven identified members (STAT1, STAT2, STAT3, STAT4, STAT5a, STAT5b, STAT6). STATs are associated with several biological processes such as cell proliferation, invasion, and metastasis in var...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170650
更新日期:2017-07-16 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20150084
更新日期:2015-05-22 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181304
更新日期:2018-12-21 00:00:00
abstract::The purpose of the study is to investigate the correlation between the expression of C-reactive protein (CRP) and autophagy-related 9B (ATG9B) and pathological features of clear cell renal cell carcinoma (CCRCC) patients. We also intended to explore the effects of manipulated expression of CRP and ATG9B on the apoptos...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20171082
更新日期:2017-11-15 00:00:00
abstract::The tetracycline repressor (TetR)-regulated system is a widely used tool to study gene functions through control of its expression. Various effectors such as tetracycline (Tc) and doxycycline (Dox) quickly induce or shut down gene expression, but reversing gene expression has not been eligible due to long half-lives o...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180793
更新日期:2019-01-08 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章,meta分析
doi:10.1042/BSR20190845
更新日期:2019-12-20 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20171529
更新日期:2018-06-12 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119802
更新日期:1989-10-01 00:00:00
abstract::A range of different types of interactions at biological membrane surfaces have been studied using various different spin label electron spin resonance (ESR) techniques. These include: (1) the interfacial ionization of local anaesthetics, (2) the binding of peripheral membrane proteins, (3) the membrane insertion of t...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1023/a:1020590122846
更新日期:1999-08-01 00:00:00
abstract::Objectives In the present study, we examined available articles from online databases to comprehensively investigate the effect of the XPC (xeroderma pigmentosum complementation group C) rs2228000 polymorphism on the risk of different types of clinical cancer. Methods We conducted a group of overall and subgroup pooli...
journal_title:Bioscience reports
pub_type: 杂志文章,meta分析
doi:10.1042/BSR20192452
更新日期:2019-12-20 00:00:00
abstract::Cervical carcinoma is the most common gynecological cancer in women worldwide. Emerging evidence has shown that long non-coding RNAs (lncRNAs) participate in multiple biological processes of cervical carcinoma tumorigenesis. We aimed to investigate the function of a novel lncRNA RP11-284F21.9 in cervical carcinoma. We...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200784
更新日期:2020-09-30 00:00:00
abstract::31P NMR studies on microorganisms have been carried out with the cells embedded in agarose gel. The novel use of the gel for the NMR studies has advantages over the usual liquid suspensions in terms of improved reproducibility of data and cell viability, with no net loss of spectral quality. Polyphosphate formation in...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117011
更新日期:1981-02-01 00:00:00
abstract::In recent decades, many genome-wide association studies on insomnia have reported numerous genes harboring multiple risk variants. Nevertheless, the molecular functions of these risk variants conveying risk to insomnia are still ill-studied. In the present study, we integrated GWAS summary statistics (N=386,533) with ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20201084
更新日期:2020-09-30 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116904
更新日期:1985-04-01 00:00:00
abstract::This is the first report of its kind that well demonstrates that a lectin from Phytolacca americana [Pa-2 (P. americana lectin-2)] can also be intrinsically unordered, based on the results obtained by CD, tryptophan fluorescence, ANS (8-anilinonaphthalene-1-sulfonic acid) binding, acrylamide quenching, DLS (dynamic li...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20090035
更新日期:2009-11-23 00:00:00
abstract::Since the Protein Data Bank (PDB) was founded in 1971, there are now over 120,000 depositions, the majority of which are from X-ray crystallography and 90% of those made use of synchrotron beamlines. At the Cambridge Structure Database (CSD), founded in 1965, there are more than 800,000 'small molecule' crystal struct...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1042/BSR20170204
更新日期:2017-07-04 00:00:00