Abstract:
:The tetracycline repressor (TetR)-regulated system is a widely used tool to study gene functions through control of its expression. Various effectors such as tetracycline (Tc) and doxycycline (Dox) quickly induce or shut down gene expression, but reversing gene expression has not been eligible due to long half-lives of such effectors. Here, we found that procaspase activating compound 1 (PAC-1) rapidly reduces transient expression of TetR-regulated green fluorescent protein (GFP) in mammalian cells. Next, we applied PAC-1 to control of expression of transient receptor potential melastatin 7 (TRPM7) protein, whose downstream cellular events can be monitored by cell morphological changes. We observed that PAC-1 quickly reduces TRPM7 expression, consequently affecting cell morphology regulated by TRPM7. The present study demonstrates the first small molecule that efficiently turns off the TetR-regulated gene expression in mammalian cells, thereby precisely regulating the expression level of target gene.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Song C,Kim N,Park M,Lee J,Oh KB,Sim Tdoi
10.1042/BSR20180793subject
Has Abstractpub_date
2019-01-08 00:00:00issue
1eissn
0144-8463issn
1573-4935pii
BSR20180793journal_volume
39pub_type
杂志文章abstract:BACKGROUND:MicroRNA-135 (miR-135) is a well-known non-coding RNA that has been demonstrated to participate in tumorigenesis and cancer development; however, the clinical prognostic value of miR-135 in digestive system cancers remains controversial. This meta-analysis aims to explore the potential value of miR-135 as a ...
journal_title:Bioscience reports
pub_type: 杂志文章,meta分析
doi:10.1042/BSR20190845
更新日期:2019-12-20 00:00:00
abstract::The rate of efflux of L-glutamate from renal brush-border membrane vesicles was enhanced by Na+ and by extravesicular L-glutamate, but not by D-glutamate nor analogs of L-glutamate that do not share the Na+-L-glutamate co-transport system. These results suggest that efflux was mediated by the Na+-L-glutamate carrier. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116468
更新日期:1981-09-01 00:00:00
abstract::31P NMR studies on microorganisms have been carried out with the cells embedded in agarose gel. The novel use of the gel for the NMR studies has advantages over the usual liquid suspensions in terms of improved reproducibility of data and cell viability, with no net loss of spectral quality. Polyphosphate formation in...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117011
更新日期:1981-02-01 00:00:00
abstract::In order to better understand the role of cell surface glycolipids in T lymphocyte activation, heparin was used to simultaneously modulate the expression of glycolipids and the lytic capacity of lymphocytes activated by interleukin-2. Results presented here show that heparin added at the start of a 3 day culture inhib...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01115230
更新日期:1988-08-01 00:00:00
abstract::Background: Ki67 has been a key role for the treatment options and prognosis evaluation in some kinds of tumors; however, the spatial expression of Ki67 in oral squamous cell carcinoma (OSCC) has not been fully-evaluated. Therefore, in the present study, we aimed to elucidate the prognosis value of Ki67 spatial expres...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181271
更新日期:2018-11-20 00:00:00
abstract::Prognostic significance of family with sequence similarity 83, member D (FAM83D) in hepatocellular carcinoma (HCC) patients has not been well-investigated using Gene Expression Omnibus (GEO) series and TCGA database, we compared FAM83D expression levels between tumor and adjacent tissues, and correlated FAM83D in tumo...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181640
更新日期:2019-05-07 00:00:00
abstract::The present study investigated the effects of Isorhamnetin on two types of prostate cancer cells (androgen-independent and androgen-dependent) and explored its possible mechanisms underlying such effects. Treatment with Isorhamnetin significantly inhibited cell growth and induced lactate dehydrogenase (LDH) release of...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20192826
更新日期:2020-03-27 00:00:00
abstract::Cervical carcinoma is the most common gynecological cancer in women worldwide. Emerging evidence has shown that long non-coding RNAs (lncRNAs) participate in multiple biological processes of cervical carcinoma tumorigenesis. We aimed to investigate the function of a novel lncRNA RP11-284F21.9 in cervical carcinoma. We...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200784
更新日期:2020-09-30 00:00:00
abstract::Heparin causes lysis of the multilamellar liposomes of all three charges, positive, neutral, and negative, and thus releases the entrapped [3H]glucose or chromate. The lytic effect of heparin is also observed in liposomes prepared from the lipids extracted from human red blood cells. Heparin is found to interact with ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121569
更新日期:1983-01-01 00:00:00
abstract::Blood pressure (BP) is a known prognostic marker for mortality in patients on maintenance hemodialysis (MHD). However, definition of the BP and its optimal values vary essentially among different MHD populations. Our purpose was to clarify these important clinical parameters in a Chinese MHD cohort. Accordingly, we re...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200858
更新日期:2020-08-28 00:00:00
abstract::The large size of a 1.5-MDa ice-binding adhesin [MpAFP (Marinomonas primoryensis antifreeze protein)] from an Antarctic Gram-negative bacterium, M. primoryensis, is mainly due to its highly repetitive RII (Region II). MpAFP_RII contains roughly 120 tandem copies of an identical 104-residue repeat. We have previously d...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140083
更新日期:2014-07-04 00:00:00
abstract::Myocardial ischemia/reperfusion (MI/R) triggers a complicated chain of inflammatory reactions. Dexmedetomidine (Dex) has been reported to be important in myocardial disorders. We evaluated the role of Dex in MI/R injury via the silent information regulator factor 2-related enzyme 1 (SIRT1)/mammalian target of rapamyci...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20194030
更新日期:2020-05-29 00:00:00
abstract:BACKGROUND:Breast cancer (BC) is the most common malignancy among females worldwide. The tumor microenvironment usually prevents effective lymphocyte activation and infiltration, and suppresses infiltrating effector cells, leading to a failure of the host to reject the tumor. CC chemokines play a significant role in in...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20202042
更新日期:2020-11-27 00:00:00
abstract::Native bovine parathyroid hormone (bPTH) was found to be readily cleaved with human leukocyte elastase to yield the fragments bPTH(1-41) and bPTH(42-84). These were then isolated by reverse-phase HPLC and characterised by gas-phase sequencing and amino acid analysis. The biological activities of these fragments were a...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122726
更新日期:1987-01-01 00:00:00
abstract::Wilms tumor is the most common renal malignancy that occurs in children. TP53 gene is considered as a tumor-suppressing gene through controlling cell growth. TP53 gene rs1042522 C>G (Arg72Pro) polymorphism is widely investigated in various types of cancers. However, it is not established if TP53 rs1042522 C>G polymorp...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181891
更新日期:2019-01-22 00:00:00
abstract::Brain metastases are the most common intracranial tumors in adults. They usually originate from: lung, breast, renal cell and gastrointestinal cancers, as well as melanoma. Prognosis for brain metastases is still poor and classical treatment combining surgery and radiation therapy should be strongly supported with mol...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180731
更新日期:2018-10-22 00:00:00
abstract::Type 2 diabetes mellitus (T2DM) complicated with non-alcoholic fatty liver disease (NAFLD) is difficult to treat. The present study explored the efficacy of (liraglutide) Lira in treating T2DM complicated with NAFLD. A total of 127 patients suffering from T2DM complicated with NAFLD were enrolled in the present study,...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181304
更新日期:2018-12-21 00:00:00
abstract::eNOS (endothelial nitric oxide synthase) contains a MAPK (mitogen-activated protein kinase)-binding site associated with a major eNOS control element. Purified ERK (extracellular-signal-regulated kinase) phosphorylates eNOS with a stoichiometry of 2-3 phosphates per eNOS monomer. Phosphorylation decreases NO synthesis...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140015
更新日期:2014-09-17 00:00:00
abstract::Sendai virus envelopes (SVE) were isolated from Sendai virus particles by Triton X-100 solubilization and ultracentrifugation. The envelopes were reconstituted in the presence of the fluorescent dye calcein by gradual removal of the detergent with Bio-beads SM-2. The internal volume of reconstituted Sendai virus envel...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121911
更新日期:1984-07-01 00:00:00
abstract::In recent decades, many genome-wide association studies on insomnia have reported numerous genes harboring multiple risk variants. Nevertheless, the molecular functions of these risk variants conveying risk to insomnia are still ill-studied. In the present study, we integrated GWAS summary statistics (N=386,533) with ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20201084
更新日期:2020-09-30 00:00:00
abstract::Prostate cancer (PCa), the second most mortal cancer from developed countries, presents a high level of chemoresistance. There is emerging evidence underscores the critical role of autophagy in the onset, progression, and chemoresistance of PCa. In the present study, we investigated the possible role of a novel autoph...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170770
更新日期:2019-08-23 00:00:00
abstract::In the present study, we investigated the role of lncRNA SAMMSON in hepatocellular carcinoma (HCC). We found that SAMMSON was up-regulated in HCC tissues, and patients with high levels of SAMMSON in HCC tissues had significantly lower overall rate within 5 years after admission. miR-9-3p was down-regulated in HCC tiss...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190615
更新日期:2019-07-05 00:00:00
abstract::The nature of certain forms of memory is discussed in relation to neural networks and REM sleep. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117331
更新日期:1988-12-01 00:00:00
abstract::Increasing studies are indicating that long non-coding RNA (lncRNA) X-inactive specific transcript (XIST) is associated with the prognosis of cancer patients. However, the results have been disputed. Therefore, we aimed to further explore the prognostic value and clinical significance of XIST in various types of cance...
journal_title:Bioscience reports
pub_type: 杂志文章,meta分析,评审
doi:10.1042/BSR20180169
更新日期:2018-06-21 00:00:00
abstract:OBJECTIVE:The current study aimed to investigate the functional roles and clinical significance of microRNA-148a (miR-148a) in the progression of oral squamous cell carcinoma (OSCC). METHODS:Relative expression of miR-148a in OSCC cells and tissues were detected using quantitative real-time polymerase chain reaction (...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20182458
更新日期:2020-04-30 00:00:00
abstract::NUF2 (NUF2, Ndc80 kinetochore complex component) plays an important role in kinetochore-microtubule attachment. It has been reported that NUF2 is associated with multiple human cancers. However, the functional role of NUF2 in pancreatic cancer remains unclear. In this study, we found that NUF2 expression was stronger ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140124
更新日期:2015-01-14 00:00:00
abstract::Hepatocellular carcinoma (HCC) has a high recurrence rate and poor clinical outcome after currently used therapies, including radiofrequency ablation. To explore the possible mechanisms for the relapse of HCC, in the present study we focussed on long non-coding RNA (LncRNA), which has been reported to be involved in t...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180896
更新日期:2019-11-29 00:00:00
abstract::C3G (Crk SH3-domain-binding guanine-nucleotide-releasing factor) is a ubiquitously expressed member of a class of molecules called GEFs (guanine-nucleotide-exchange factor) that activate small GTPases and is involved in pathways triggered by a variety of signals. It is essential for mammalian embryonic development and...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1042/BSR20100094
更新日期:2011-08-01 00:00:00
abstract::Whole-genome analyses become more and more necessary for pharmaceutical research. DNA chip hybridizations are an important tool for monitoring gene expression profiles during diseases or medical treatment. However, drug target identification and validation as well as an increasing number of antibodies and other polype...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/s10540-005-2848-y
更新日期:2005-02-01 00:00:00
abstract::The present study describes the enzymatic properties and molecular identification of 5'-nucleotidase in soluble and microsomal fractions from rat cardiac ventricles. Using AMP as a substrate, the results showed that the cation and the concentration required for maximal activity in the two fractions was magnesium at a ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20070039
更新日期:2008-10-01 00:00:00