Abstract:
:Disc nucleus pulposus (NP) cell experiences periodic osmolarity alterations during daily activities, which has been proved to affect cell biology in vitro The present study was aimed to investigate the effects of cartilage-derived morphogenetic protein-2 (CDMP-2) on NP cell apoptosis under the hyperosmolarity culture and the potential mechanism. Isolated rat NP cells were cultured in the in situ-osmolarity medium or hyperosmolarity medium for 3 days. CDMP-2 was added into the hyperosmolarity medium to investigate its effects on NP cell apoptosis. Cell apoptosis rate, caspase-3 activity, gene expression of Bcl-2, Bax, and caspase-3, and protein expression of Bcl-2, Bax, and cleaved caspase-3 were analyzed to evaluate NP cell apoptosis. Additionally, the intracellular reactive oxygen species (ROS) and the total superoxide dismutase (SOD) activity were analyzed to investigate the potential role of oxidative damage in this process. In the hyperosmolarity culture, NP cells showed a significantly increased cell apoptosis rate and caspase-3 activity, an up-regulated expression of Bax and caspase-3/cleaved-caspase-3 and a down-regulated expression of Bcl-2. However, CDMP-2 partly inhibited these effects of hyperosmolarity culture on NP cells. Additionally, the hyperosmolarity culture significantly increased ROS content and decreased the total SOD activity compared with the in situ-osmolarity culture, whereas exogenous CDMP-2 partly decreased the ROS content and increased the total SOD activity in the hyperosmolarity culture. In conclusion, CDMP-2 is effective in attenuating hyperosmolarity environment-induced NP cell apoptosis, and this process may be mediated through inhibiting oxidative stress damage. The present study indicates that CDMP-2 may be helpful to retard hyperosmolarity niche-mediated disc degeneration.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Jiao S,Li J,Liu B,Yang M,Xiu J,Qu Ddoi
10.1042/BSR20181176subject
Has Abstractpub_date
2018-10-09 00:00:00issue
5eissn
0144-8463issn
1573-4935pii
BSR20181176journal_volume
38pub_type
杂志文章abstract::Stromal cell derived factor-1 (SDF-1) and basic fibroblast growth factor (bFGF) were reported to induce the differentiation of bone marrow stem cells (BMSCs) into cells with characteristics of periodontal ligament fibroblasts. Thus SDF-1 and bFGF may play a positive role in BMSCs-mediated periodontal ligament regenera...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190785
更新日期:2019-12-20 00:00:00
abstract::We investigated the possible translational role which elevated concentrations of highly purified Semliki Forest virus (SFV) capsid (C)-protein molecules may play in a cell-free translation system. Here we demonstrate that in the absence of double-stranded RNA high concentrations of C protein triggered the phosphorylat...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01198464
更新日期:1996-12-01 00:00:00
abstract::Objective: We aimed to assess the possible relations between serum levels of macrophage migration inhibitory factor (MIF), a central cytokine of the innate immunity and inflammatory response, and benign paroxysmal positional vertigo (BPPV) risk and BPPV recurrence events.Methods: In the present study, 154 patients wit...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20191831
更新日期:2019-08-23 00:00:00
abstract::This article discusses how changes in luminal calcium concentration affect calcium release rates from triad-enriched sarcoplasmic reticulum vesicles, as well as single channel opening probability of the ryanodine receptor/calcium release channels incorporated in bilayers. The possible participation of calsequestrin, o...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/BF01788370
更新日期:1995-10-01 00:00:00
abstract::Cervical cancer (CC) is ranked as the fourth most common cancer that occurs in women universally, which normally causes pain in the lower belly. Plenty of studies have stated that the expression of long non-coding RNAs (lncRNAs) is linked to the cellular development of many kinds of cancers. DSCAM-AS1 has been reporte...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20192061
更新日期:2020-01-31 00:00:00
abstract::Bifunctional dihaem cytochrome c thiosulfate dehydrogenases/tetrathionate reductases (TsdA) exhibit different catalytic properties depending on the source organism. In the human food-borne intestinal pathogen Campylobacter jejuni, TsdA functions as a tetrathionate reductase enabling respiration with tetrathionate as a...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160457
更新日期:2016-12-09 00:00:00
abstract::The neurotransmitter, serotonin has emerged as a tumor growth factor and immune response regulator through complex signaling pathways. Yip1 Interacting Factor Homolog B (YIF1B) is a membrane protein involved in serotonin receptor (HTR) membrane trafficking and signal transmission in neuropathy. Participation of YIF1B ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20201384
更新日期:2020-07-31 00:00:00
abstract::Prefoldin subunits (PFDN), primarily known for co-chaperone function associated with cytoskeletal rearrangement, have been found involved in epithelial-mesenchymal transition (EMT) and cancer progression. However, studies focusing on the roles of PFDN in gastric cancer (GC) remain limited. The present study aims to ev...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20192712
更新日期:2020-02-28 00:00:00
abstract::Ric c1, an allergenic protein from Ricinus communis , is an insect α-amylase inhibitor that has become an occupational allergen. Ric c1 can cross-react with allergens from wheat, soybean, peanut, shrimp, fish, gluten, house dust, tobacco, and air fungus, thereby amplifying the concern and risks caused by Ricinus aller...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20171245
更新日期:2018-02-14 00:00:00
abstract::The 7-nitro-2,1,3-nitrobenzoxadiazole (NBD) derivatives are a series of compounds containing the NBD scaffold that are not glutathione (GSH) peptidomimetics, and result in a strong inhibition of glutathione S-transferases (GSTs). Growing evidences highlight their pivotal roles and outstanding anticancer activity in di...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1042/BSR20171440
更新日期:2018-02-13 00:00:00
abstract::The effect that research on dopamine and other neutro trasmitters has on disciplines like neurology and psychiatry is described. ...
journal_title:Bioscience reports
pub_type: 传,历史文章
doi:10.1023/a:1015556204669
更新日期:2001-12-01 00:00:00
abstract::miRNAs are non-coding RNA molecules typically 18-22 nucleotides long that can suppress the expression of their target genes. Several laboratories have attempted to identify miRNAs from the pig that are involved in Salmonella infection. These bioinformatics strategies using the newly available genomic sequence are gene...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160243
更新日期:2016-08-31 00:00:00
abstract::Circulating tumor cells can provide important diagnostic and prognostic information of the patients with non-small cell lung cancer (NSCLC). Aldehyde dehydrogenase 1 (ALDH1), a cancer stem cell marker, has been used in various tumors, including NSCLC. In the present study, we isolated the circulating ALDH1+ tumor cell...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180914
更新日期:2018-10-31 00:00:00
abstract::Recent developments in the study of peptide amidation are reviewed. The main areas covered are assay procedures, purification of amidating enzymes, co-factors and regulation, mechanism and specificity of the amidating reaction, and multiple forms of the amidating enzyme and glycosylation. Discussion is presented on as...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/BF01122123
更新日期:1987-12-01 00:00:00
abstract::Although wasting marmoset syndrome (WMS) is one of the biggest problems facing captive marmoset colonies, the mechanisms underlying its pathogenesis remain unclear. In our clinical experience, it is difficult to cure WMS-affected marmosets with severe hypoalbuminemia. Thus, the mechanisms underlying hypoalbuminemia in...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190562
更新日期:2019-07-08 00:00:00
abstract::Due to poor prognosis of glioblastoma (GBM), there is an urgent need to develop new therapeutic strategies. Besides eliminating GBM tumor cells and stem cells, a novel therapeutic approach aims to target Glioma-associated microglia/macrophages (GAMs). We investigated the molecular profile of GAMs correlated with patie...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20182361
更新日期:2019-06-20 00:00:00
abstract::Kinesin family member 14 (KIF14) is a member of kinesin family proteins which have been found to be dysregulated in various cancer types. However, the expression of KIF14 and its potential prognostic significance have not been investigated in cervical cancer. Real-time PCR was performed to assess the expression levels...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20150314
更新日期:2016-04-05 00:00:00
abstract::Three myoid-cell clones were established from the thymuses of two Wistar rats; one thymus yielded two clones, R615A and R615B2, and the other yielded one clone, R613Ad. The three clones were divided into two subtypes. Both subtypes were able to form myofibrils, expressed AChR on their cell-surface membrane, and contai...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116890
更新日期:1984-11-01 00:00:00
abstract::It is well known that the amino acids in the blood are distributed between the plasma and inside the cells. This study was conducted to determine whether amino acids can be located adsorbed on blood cell membranes. The amino acid concentration in the deproteinized haemolysed blood was higher than that in the fraction ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01136856
更新日期:1991-08-01 00:00:00
abstract::A model explaining quantal Ca2+ release as an intrinsic property of the inositol 1,4,5-triphosphate (IP3) receptor has been put forward. The model is based on the hypothesis that the IP3 receptor can catalyze a transformation of the IP3 molecule differing from its conventional metabolism. A simple kinetic mechanism is...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01207265
更新日期:1996-10-01 00:00:00
abstract:OBJECTIVE:Gastric cancer is the most common gastrointestinal malignancy in China and results from a combination of genetic and environmental factors. The present study was conducted to investigate the relationship between long noncoding RNA (lncRNA) materally expressed gene 3 (MEG3) single nucleotide polymorphisms (SNP...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200305
更新日期:2020-11-27 00:00:00
abstract::Hepatocellular carcinoma (HCC) has a high recurrence rate and poor clinical outcome after currently used therapies, including radiofrequency ablation. To explore the possible mechanisms for the relapse of HCC, in the present study we focussed on long non-coding RNA (LncRNA), which has been reported to be involved in t...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180896
更新日期:2019-11-29 00:00:00
abstract::Cholera toxin (CT) is composed of a disulfide-linked A1/A2 heterodimer and a ring-like, cell-binding B homopentamer. The catalytic A1 subunit must dissociate from CTA2/CTB5 to manifest its cellular activity. Reduction of the A1/A2 disulfide bond is required for holotoxin disassembly, but reduced CTA1 does not spontane...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181320
更新日期:2018-09-07 00:00:00
abstract::Abnormal aggregation of pathological tau protein is a neuropathological feature of Alzheimer's disease (AD). In the AD patients, the abnormal tau accumulation first appeared in entorhinal cortex (EC) and then propagated to the hippocampus with microglia activation and inflammation, but the mechanism is elusive. Here, ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20193629
更新日期:2020-02-28 00:00:00
abstract::The molecular mechanism of the occurrence and development of papillary thyroid carcinoma (PTC) has been widely explored, but has not been completely elucidated. The present study aimed to identify and analyze genes associated with PTC by bioinformatics methods. Two independent datasets were downloaded from Gene Expres...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190083
更新日期:2019-04-02 00:00:00
abstract::Clustered Regularly Interspaced Short Palindromic Repeat activation-synergistic activation mediator system (CRISPRa-SAM) has been efficiently used to up-regulate the targeted genes in human and mouse. But it is not known whether the CRISPRa-SAM system can be used against porcine disease because its two important trans...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20191496
更新日期:2019-08-19 00:00:00
abstract::A slow phase of linear exocytosis by fibroblasts of previously endocytosed fluid, succeeding the initial burst of release, is described. This is not due to cell lysis, is virtually abolished at 4 degrees C, and is retarded when the lysosomal system is expanded. It may reflect membrane recycling. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01314215
更新日期:1982-08-01 00:00:00
abstract::Circulating human neutrophils exhibited low rates of protein biosynthesis, as determined by their ability to incorporate [35S]methionine into TCA-precipitable material. Exposure of cells to the chemotactic peptide (N-formyl-L-methionyl-L-leucyl-L-phenylalanine) increased their rate of protein synthesis, and the maxima...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119479
更新日期:1987-11-01 00:00:00
abstract::We have investigated a novel method to monitor real changes of intracellular ROS by the use of CMH2TMRos (a reduced form of MitoTracker orange) in Swiss 3T3 fibroblasts. Arachidonic acid induced a rapid increase of CMTMRos fluorescence with a maximal elevation at 120-150 sec, which was determined by scanning every 10 ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1023/a:1013290316939
更新日期:2001-06-01 00:00:00
abstract::The Gram-positive bacterium Staphylococcus pseudintermedius is a leading cause of canine bacterial pyoderma, resulting in worldwide morbidity in dogs. S. pseudintermedius also causes life-threatening human infections. Furthermore, methicillin-resistant S. pseudintermedius is emerging, resembling the human health threa...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140088
更新日期:2014-11-24 00:00:00