Abstract：
:To observe the effect of gene expression and tumorigenicity in hybrid cells of human embryonic stem cells (hESCs) and ovarian cancer cells in vitro and in vivo using a mouse model, and to determine its feasibility in reprogramming tumour cells growth and apoptosis, for a potential exploration of the role of hESCs and tumour cells fusion in the management of ovarian cancer. Stable transgenic hESCs (H1) and ovarian cancer cell line OVCAR-3 were established before fusion, and cell fusion system was established to analyse the related indicators. PTEN expression in HO-H1 cells was higher than those in the parental stem cells and lower than those in parental tumour cells; the growth of OV-H1 (RFP+GFP) hybrid cells with double fluorescence expressions were obviously slower than that of human embryonic stem cells and OVCAR-3 ovarian cancer cells. The apoptosis signal of the OV-H1 hybrid cells was significantly higher than that of the hESCs and OVCAR-3 ovarian cancer cells. In vivo results showed that compared with 7 days, 28 days and 35 days after inoculation of OV-H1 hybrid cells; also, apoptotic cell detection indicated that much stronger apoptotic signal was found in OV-H1 hybrid cells inoculated mouse. The hESCs can inhibit the growth of OVCAR-3 cells in vitro by suppressing p53 and PTEN expression to suppress the growth of tumour that may be achieved by inducing apoptosis of OVCAR-3 cells. The change of epigenetics after fusion of ovarian cancer cells and hESCs may become a novel direction for treatment of ovarian cancer.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
He K,Qu H,Xu LN,Gao J,Cheng FY,Xiang P,Zhou CQdoi
10.1042/BSR20160104subject
Has Abstractpub_date
2016-09-16 00:00:00issue
5eissn
0144-8463issn
1573-4935pii
BSR20160104journal_volume
36pub_type
杂志文章abstract：:Evidence is presented in support of a pathway in skeletal muscle of glyconeogenesis (glycogen biosynthesis de novo) from L-glutamate and related amino acids involving the enzyme phosphoenolpyruvate carboxykinase (PEP CK). In the rat hemidiaphragm in vitro, not only did L-[U]-14C]glutamate exert a glycogen-sparing acti...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01117013
更新日期：1981-02-01 00:00:00
abstract：:The role of the topoisomerase enzyme in DNA recombination was investigated by extracting chromosomal deoxyribonucleoproteins from a variety of cultured mammalian cells and assaying for the formation of recombinant DNA structures. Although each of the crude deoxyribonucleoprotein preparations contained topoisomerase ac...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01115252
更新日期：1982-07-01 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
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更新日期：2020-08-28 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章,收录出版
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更新日期：2018-09-13 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01124790
更新日期：1987-03-01 00:00:00
abstract：:The cytosolic Ca2+ activity of insulin-releasing clonal cells (RINm5F) was studied with the intracellular fluorescent indicator quin-2. When the extracellular Ca2+ concentration was 1 mM, the basal cytosolic Ca2+ activity was 101 +/- 5 nM. Depolarization with 25 mM K+ increased this Ca2+ activity to at least 318 nM, a...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01140663
更新日期：1983-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1042/BSR20160226
更新日期：2016-11-03 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1023/a:1013290316939
更新日期：2001-06-01 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20202042
更新日期：2020-11-27 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
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更新日期：2017-07-27 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20200784
更新日期：2020-09-30 00:00:00
abstract：:Circulating tumor cells can provide important diagnostic and prognostic information of the patients with non-small cell lung cancer (NSCLC). Aldehyde dehydrogenase 1 (ALDH1), a cancer stem cell marker, has been used in various tumors, including NSCLC. In the present study, we isolated the circulating ALDH1+ tumor cell...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20180914
更新日期：2018-10-31 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20203944
更新日期：2021-01-29 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01116383
更新日期：1982-03-01 00:00:00
abstract：:The emergence of multidrug-resistant (MDR) microbes caused by overuse of antibiotics leads to urgent demands for novel antibiotics exploration. Our recent data showed that Ly2.1-3 (a novel lymphocyte antigen 6 (Ly6) gene cluster) were proteins with cationic nature and rich in cysteine content, that are characteristic ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20160265
更新日期：2017-01-17 00:00:00
abstract：:The influence of lindane upon the dynamic properties of plasma membranes from rat ventral prostate has been investigated using a fluorescence polarization technique. Preincubation with lindane decreased the fluorescence polarization in a dose dependent manner. This effect, which is associated with an increased membran...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01119197
更新日期：1991-04-01 00:00:00
abstract：:In the course of evolution, mitochondria lost their independence, and mtDNA became "slave" of nDNA, depending on numerous nucleus-encoded factors for its integrity, replication and expression. Mutations in any of these factors may alter the cross-talk between the two genomes and cause diseases that affect mtDNA integr...
journal_title：Bioscience reports
pub_type： 杂志文章,评审
doi：10.1007/s10540-007-9036-1
更新日期：2007-06-01 00:00:00
abstract：:Bleaching of the purple membrane strongly reduces the number of divalent cation binding sites as well as their affinities. Conversely, deionization of the bleached membrane drastically inhibits the chromophore regeneration. Proteolysis experiments using bromelain show that the bleached membrane has an additional cleav...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01114972
更新日期：1986-11-01 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01117344
更新日期：1988-12-01 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01119802
更新日期：1989-10-01 00:00:00
abstract：:It is well known that the amino acids in the blood are distributed between the plasma and inside the cells. This study was conducted to determine whether amino acids can be located adsorbed on blood cell membranes. The amino acid concentration in the deproteinized haemolysed blood was higher than that in the fraction ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01136856
更新日期：1991-08-01 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01116866
更新日期：1987-09-01 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20200936
更新日期：2020-12-23 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01114689
更新日期：1989-06-01 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20181640
更新日期：2019-05-07 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20160174
更新日期：2016-09-16 00:00:00
abstract：:Hematopoietic pre-B cell leukemia transcription factor (PBX)-interacting protein (RRBP1) has been shown to participate in various aspects of malignancies. The clinical significance of RRBP1 and its involvement in the epithelial ovarian cancer have yet to be studied. The aim of the present study was to investigate the ...
journal_title：Bioscience reports
pub_type： 临床试验,杂志文章
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更新日期：2019-07-23 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20140012
更新日期：2014-04-01 00:00:00
abstract：:Calpain-1 and calpain-2 are highly structurally similar isoforms of calpain. The calpains, a family of intracellular cysteine proteases, cleave their substrates at specific sites, thus modifying their properties such as function or activity. These isoforms have long been considered to function in a redundant or comple...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20200552
更新日期：2020-11-27 00:00:00
abstract：:Imipramine and clorgyline, at concentrations of 0.002 M, inhibit the respiration of brain tissue by 82 and 71 per cent respectively, while chloropromazine and tranylcypromine, at concentrations of 0.01 M, inhibit it about 25 per cent. Deprenyl and amphetamine at a concentration of 0.002 M inhibit brain tissue respirat...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01118600
更新日期：1991-02-01 00:00:00
