Isorhamnetin inhibited the proliferation and metastasis of androgen-independent prostate cancer cells by targeting the mitochondrion-dependent intrinsic apoptotic and PI3K/Akt/mTOR pathway.


:The present study investigated the effects of Isorhamnetin on two types of prostate cancer cells (androgen-independent and androgen-dependent) and explored its possible mechanisms underlying such effects. Treatment with Isorhamnetin significantly inhibited cell growth and induced lactate dehydrogenase (LDH) release of androgen-independent DU145 and PC3 prostate cancer cells, but exhibited almost no toxicity effect on androgen-dependent LNCaP prostate cancer cell line or normal human prostate epithelial PrEC cells, which was achieved by the induction of apoptosis in a mitochondrion-dependent intrinsic apoptotic pathway. Furthermore, Isorhamnetin inhibited cell migration and invasion in concentration-dependent manners by enhancing mesenchymal-epithelial transition (MET) and inhibiting matrix metalloproteinase (MMP) 2 (MMP-2) and MMP-9 overexpression. In addition, Isorhamnetin also down-regulated the expression of phosphorylated PI3K (p-P13K), Akt (p-Akt), and mTOR (p-mTOR) proteins in both cancer cells, revealing Isorhamnetin to be a selective PI3K-Akt-mTOR pathway inhibitor. In summary, these findings propose that Isorhamnetin might be a novel therapeutic candidate for the treatment of androgen-independent prostate cancer.


Biosci Rep


Bioscience reports


Cai F,Zhang Y,Li J,Huang S,Gao R




Has Abstract


2020-03-27 00:00:00












  • Evidence on the conformation of HeLa-cell 5.8 S rRNA during interaction with 28 S rRNA.

    abstract::The sites and extents of bisulphite-mediated deamination of cytidine residues in HeLa-cell 5.8 S rRNA within the 5.8 S / 28 S rRNA complex have been determined. Comparison of these data with equivalent data for isolated ('unbound') 5.8 S rRNA suggests that the conformation of several cytidine residues, including inter...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Kelly JM,Goddard JP

    更新日期:1981-05-01 00:00:00

  • HPO-Shuffle: an associated gene prioritization strategy and its application in drug repurposing for the treatment of canine epilepsy.

    abstract::Epilepsy is a common neurological disorder that affects mammalian species including human beings and dogs. In order to discover novel drugs for the treatment of canine epilepsy, multiomics data were analyzed to identify epilepsy associated genes. In this research, the original ranking of associated genes was obtained ...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Wang S,Meng X,Wang Y,Liu Y,Xia J

    更新日期:2019-09-06 00:00:00

  • Ultrasound reverses chemoresistance in breast cancer stem cell like cells by altering ABCG2 expression.

    abstract::Doxorubicin (DOX) resistance in breast cancer largely results from the breast cancer stem cell like cells (BCSCs) which could be targetted to improve the efficacy of chemotherapy. Cell permeabilization using microbubbles (MBs) and ultrasound (US) have the potential for delivering molecules into the cytoplasm. We aim t...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Guo L,Zheng P,Fan H,Wang H,Xu W,Zhou W

    更新日期:2017-11-09 00:00:00

  • Efficacy of liraglutide in treating type 2 diabetes mellitus complicated with non-alcoholic fatty liver disease.

    abstract::Type 2 diabetes mellitus (T2DM) complicated with non-alcoholic fatty liver disease (NAFLD) is difficult to treat. The present study explored the efficacy of (liraglutide) Lira in treating T2DM complicated with NAFLD. A total of 127 patients suffering from T2DM complicated with NAFLD were enrolled in the present study,...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Tian F,Zheng Z,Zhang D,He S,Shen J

    更新日期:2018-12-21 00:00:00

  • Antigen delivery to mucosa-associated lymphoid tissues using liposomes as a carrier.

    abstract::Oral vaccination requires an antigen delivery vehicle to protect the antigen and to enhance translocation of the antigen to the mucosa-associated lymphoid tissue. A variety of antigen delivery vehicles including liposomes have been studied for mucosal immunization. The advantages of liposome formulations are their par...

    journal_title:Bioscience reports

    pub_type: 杂志文章,评审


    authors: Zho F,Neutra MR

    更新日期:2002-04-01 00:00:00

  • Ca-ATPase activity and protein composition of sarcoplasmic reticulum membranes isolated from skeletal muscles of typical hibernator, the ground squirrel Spermophilus undulatus.

    abstract::Ca-ATPase activity in sarcoplasmic reticulum (SR) membranes isolated from skeletal muscles of the typical hibernator, the ground squirrel Spermophilus undulatus, is about 2-fold lower than that in SR membranes of rats and rabbits and is further decreased 2-fold during hibernation. The use of carbocyanine anionic dye S...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Malysheva AN,Storey KB,Lopina OD,Rubtsov AM

    更新日期:2001-12-01 00:00:00

  • microRNA-613 exerts anti-angiogenic effect on nasopharyngeal carcinoma cells through inactivating the AKT signaling pathway by down-regulating FN1.

    abstract::Background: Nasopharyngeal carcinoma (NPC) is a disease highly sensitive to radiotherapy with the unclear etiology. However, the specific effects of microRNA-613 (miR-613) on NPC still remain elusive. Therefore, the present study probes into the underlying mechanism of miR-613 in NPC via AKT signaling pathway by regul...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Gao R,Feng Q,Tan G

    更新日期:2019-07-10 00:00:00

  • Long non-coding RNA DSCAM-AS1 contributes to the tumorigenesis of cervical cancer by targeting miR-877-5p/ATXN7L3 axis.

    abstract::Cervical cancer (CC) is ranked as the fourth most common cancer that occurs in women universally, which normally causes pain in the lower belly. Plenty of studies have stated that the expression of long non-coding RNAs (lncRNAs) is linked to the cellular development of many kinds of cancers. DSCAM-AS1 has been reporte...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Liang J,Zhang S,Wang W,Xu Y,Kawuli A,Lu J,Xiu X

    更新日期:2020-01-31 00:00:00

  • Epithelial barrier function properties of the 16HBE14o- human bronchial epithelial cell culture model.

    abstract::The human bronchial epithelial cell line, 16HBE14o- (16HBE), is widely used as a model for respiratory epithelial diseases and barrier function. During differentiation, transepithelial electrical resistance (TER) increased to approximately 800 Ohms × cm2, while 14C-d-mannitol flux rates (Jm) simultaneously decreased. ...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Callaghan PJ,Ferrick B,Rybakovsky E,Thomas S,Mullin JM

    更新日期:2020-10-30 00:00:00

  • A putative pathway of glyconeogenesis in skeletal muscle.

    abstract::Evidence is presented in support of a pathway in skeletal muscle of glyconeogenesis (glycogen biosynthesis de novo) from L-glutamate and related amino acids involving the enzyme phosphoenolpyruvate carboxykinase (PEP CK). In the rat hemidiaphragm in vitro, not only did L-[U]-14C]glutamate exert a glycogen-sparing acti...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Odedra BR,Palmer TN

    更新日期:1981-02-01 00:00:00

  • The tumor-suppressive function of miR-1296-5p by targeting EGFR and CDK6 in gastric cancer.

    abstract::We aimed to confirm the role of miR-1296-5p in gastric cancer and to identify its target genes. The expression of miR-1296-5p was measured in gastric cancer tissues and cell lines. The function of miR-1296-5p was examined by the overexpression and inhibition of its expression in typical gastric cell lines as well as S...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Jia Y,Zhao LM,Bai HY,Zhang C,Dai SL,Lv HL,Shan BE

    更新日期:2019-01-08 00:00:00

  • Microstructure changes in whiter matter relate to cognitive impairment in Wilson's disease.

    abstract::Purpose: Wilson's disease (WD) is a genetic disorder of copper metabolism with pathological copper accumulation in the brain. The purpose of the present study was to evaluate the relationship between the damaged white matter and the impaired cognitive function in WD patients. Materials and methods: Thirty WD adolescen...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Dong T,Yang WM,Wu MC,Zhang J,Huang P,Xu CS,Wang AQ,Kuang CJ,Gao ZL

    更新日期:2019-03-15 00:00:00

  • Identification of novel biomarkers involved in pulmonary arterial hypertension based on multiple-microarray analysis.

    abstract::Pulmonary arterial hypertension (PAH) is a life-threatening chronic cardiopulmonary disorder. However, studies providing PAH-related gene expression profiles are scarce. To identify hub genes involved in PAH, we investigate two microarray data sets from gene expression omnibus (GEO). A total of 150 differentially expr...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Ma Y,Chen SS,Feng YY,Wang HL

    更新日期:2020-09-30 00:00:00

  • Reduction of the cytosolic calcium activity in clonal insulin-releasing cells exposed to glucose.

    abstract::The cytosolic Ca2+ activity of insulin-releasing clonal cells (RINm5F) was studied with the intracellular fluorescent indicator quin-2. When the extracellular Ca2+ concentration was 1 mM, the basal cytosolic Ca2+ activity was 101 +/- 5 nM. Depolarization with 25 mM K+ increased this Ca2+ activity to at least 318 nM, a...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Rorsman P,Berggren PO,Gylfe E,Hellman B

    更新日期:1983-10-01 00:00:00

  • Studies on the subcellular localization and role of glutathione-insulin transhydrogenase in rat liver.

    abstract::125I-insulin was shown to be internalized in vivo to a discrete population of low-density membranes (ligandosomes), distinct from the +Golgi, endoplasmic reticulum, plasma membrane, and lysosomes. However, analytical subcellular fractionation shows that glutathione-insulin transhydrogenase is localized to the endoplas...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Chowdhary BK,Smith GD,Mahler R,Peters TJ

    更新日期:1983-04-01 00:00:00

  • Quantal Ca2+ release and inactivation in a model of the inositol 1,4,5-trisphosphate receptor involving transformation of the ligand by the receptor.

    abstract::A model explaining quantal Ca2+ release as an intrinsic property of the inositol 1,4,5-triphosphate (IP3) receptor has been put forward. The model is based on the hypothesis that the IP3 receptor can catalyze a transformation of the IP3 molecule differing from its conventional metabolism. A simple kinetic mechanism is...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Kaimachnikov NP,Nazarenko VG

    更新日期:1996-10-01 00:00:00

  • Evidence that protein kinase C and mitogen activated protein kinase are not involved in the mechanism by which insulin stimulates translation in L6 myoblasts.

    abstract::Insulin stimulated a concentration-dependent increase in protein synthesis in L6 myoblasts which was significant at 1 nM. This response was not prevented by the transcription inhibitor, actinomycin D. The protein kinase C (PKC) inhibitor, Ro-31-8220, and downregulation of PKC by prolonged incubation of cells with 12-O...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Thompson MG,Pascal M,Mackie SC,Thom A,Morrison KS,Backwell FR,Palmer RM

    更新日期:1995-02-01 00:00:00

  • Phagocytosis and phagosome maturation are regulated by calcium in J774 macrophages interacting with unopsonized prey.

    abstract::Phagocytosis by neutrophils, macrophages, and other professional phagocytes requires rapid remodeling of actin. Early phagosomes are surrounded by a rim of F-actin that is disassembled during phagosomoal maturation. Breakdown of periphagosomal F-actin and phagolysosome fusion are calcium dependent processes in neutrop...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Tejle K,Magnusson KE,Rasmusson B

    更新日期:2002-10-01 00:00:00

  • Clusterin role in hepatocellular carcinoma patients treated with oxaliplatin.

    abstract:AIM:To explore the prognostic value of clusterin (CLU) in hepatocellular carcinoma (HCC) patients treated with oxaliplatin (OXA). METHODS:Relative expression of plasma CLU mRNA was examined via fluorescence quantitative real-time PCR (qRT-PCR), and CLU protein level in tissue samples was detected through immunohistoch...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Wang X,Liu Y,Qin Q,Zheng T

    更新日期:2020-02-28 00:00:00

  • Osteoprotective effect of combination therapy of low-dose oestradiol with G15, a specific antagonist of GPR30/GPER in ovariectomy-induced osteoporotic rats.

    abstract::Identified and cloned in 1996 for the first time, G protein-coupled oestrogen receptor (ER) 30 (GPR30/GPER) has been a hot spot in the field of sex hormone research till now. In the present study, we examined the effects of low-dose oestradiol (E2) combined with G15, a specific antagonist of GPR30 on ovariectomy (OVX)...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Kang WB,Cong Y,Ru JY,Ying SQ,Zhu T,Wang DS,Liu XW,Liu G,Zhao JN

    更新日期:2015-07-03 00:00:00

  • Interactions at the membrane surface studied by spin label ESR spectroscopy.

    abstract::A range of different types of interactions at biological membrane surfaces have been studied using various different spin label electron spin resonance (ESR) techniques. These include: (1) the interfacial ionization of local anaesthetics, (2) the binding of peripheral membrane proteins, (3) the membrane insertion of t...

    journal_title:Bioscience reports

    pub_type: 杂志文章,评审


    authors: Marsh D

    更新日期:1999-08-01 00:00:00

  • The effect of different concentrations of gold nanoparticles on growth performance, toxicopathological and immunological parameters of broiler chickens.

    abstract::The present study aimed to evaluate what dosage of gold nanoparticles (GNPs) would improve growth performance, antioxidant levels and immune defense in broiler chickens. The experiment was carried out on 90 one-day-old mixbred Cobb chicks. The birds were allocated into three groups with three replicates. Group (1) kep...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Hassanen EI,Morsy EA,Hussien AM,Ibrahim MA,Farroh KY

    更新日期:2020-03-27 00:00:00

  • The transcription factor USF1 promotes glioma cell invasion and migration by activating lncRNA HAS2-AS1.

    abstract:OBJECTIVE:The role of lncRNAs in tumor has been widely concerned. The present study took HAS2-AS1 (the antisense RNA 1 of HAS2) as a starting point to explore its expression in glioma and its role in the process of migration and invasion, providing a strong theoretical basis for mining potential therapeutic targets of ...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Wang J,Gu J,You A,Li J,Zhang Y,Rao G,Ge X,Zhang K,Li J,Liu X,Wang Q,Lin T,Cheng L,Zhu M,Wu X,Wang D

    更新日期:2020-08-28 00:00:00

  • LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo.

    abstract::Accumulating evidence demonstrates that long non-coding RNAs (LncRNAs) play important roles in regulating gene expression and are involved in various cancers, including colorectal cancer (CRC). However, LncRNA profiles in CRC remain largely unknown. The present study aims to find the key LncRNA associated with CRC and...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Sun L,Xue H,Jiang C,Zhou H,Gu L,Liu Y,Xu C,Xu Q

    更新日期:2016-05-06 00:00:00

  • The novel testicular enrichment protein Cfap58 is required for Notch-associated ciliogenesis.

    abstract::Cilia and flagella are critical organelles with conserved internal structures and diverse developmental and physiological processes according to cell type. Although the core components of structures are shared with thousands of associated proteins involved in cilia or flagella formation, we hypothesized that some unkn...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Li ZZ,Zhao WL,Wang GS,Gu NH,Sun F

    更新日期:2020-01-31 00:00:00

  • Identification of key genes involved in type 2 diabetic islet dysfunction: a bioinformatics study.

    abstract::Aims: To identify the key differentially expressed genes (DEGs) in islet and investigate their potential pathway in the molecular process of type 2 diabetes.Methods: Gene Expression Omnibus (GEO) datasets (GSE20966, GSE25724, GSE38642) of type 2 diabetes patients and normal controls were downloaded from GEO database. ...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Zhong M,Wu Y,Ou W,Huang L,Yang L

    更新日期:2019-05-31 00:00:00

  • miRNA-340 inhibits osteoclast differentiation via repression of MITF.

    abstract::Many miRNAs play critical roles in modulating various biological processes of osteoclast differentiation and function. Microphthalmia-associated transcription factor (MITF), a target of miR-340, served as pivotal transcription factor involved in osteoclast differentiation. However, the role of miR-340 and MITF during ...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Zhao H,Zhang J,Shao H,Liu J,Jin M,Chen J,Huang Y

    更新日期:2017-07-20 00:00:00

  • Disorders of nuclear-mitochondrial intergenomic communication.

    abstract::In the course of evolution, mitochondria lost their independence, and mtDNA became "slave" of nDNA, depending on numerous nucleus-encoded factors for its integrity, replication and expression. Mutations in any of these factors may alter the cross-talk between the two genomes and cause diseases that affect mtDNA integr...

    journal_title:Bioscience reports

    pub_type: 杂志文章,评审


    authors: Spinazzola A,Zeviani M

    更新日期:2007-06-01 00:00:00

  • Potential role of miR-155-5p in fat deposition and skeletal muscle development of chicken.

    abstract::miR-155 has multiple functions in many physiological and pathological processes. However, little is known about the expression characteristics of avian miR-155. In the present study, partial pri-miR-155 sequences were cloned from AA+ broiler, Sanhuang broiler and Hy-Line Brown layer, respectively. Stem-loop qRT-PCR wa...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Xu S,Chang Y,Wu G,Zhang W,Man C

    更新日期:2020-06-26 00:00:00

  • Variants and expression changes in PPAR-encoding genes display no significant association with schizophrenia.

    abstract::A few studies suggested the contribution of PPARs to the etiology of schizophrenia (SCZ). However, it is still not clear whether variants in PPAR-encoding genes have a direct association with SCZ. The potential linkage between SCZ and the variants within PPAR encoding genes (PPARA, PPARD, and PPARG) was tested in a la...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Li X,Zhu Y,Keaton M,Baranova A,Liu S,Hu X,Li Q,Cheng L,Zhou P,Cao H,Xu Y

    更新日期:2020-07-31 00:00:00