Isorhamnetin inhibited the proliferation and metastasis of androgen-independent prostate cancer cells by targeting the mitochondrion-dependent intrinsic apoptotic and PI3K/Akt/mTOR pathway.


:The present study investigated the effects of Isorhamnetin on two types of prostate cancer cells (androgen-independent and androgen-dependent) and explored its possible mechanisms underlying such effects. Treatment with Isorhamnetin significantly inhibited cell growth and induced lactate dehydrogenase (LDH) release of androgen-independent DU145 and PC3 prostate cancer cells, but exhibited almost no toxicity effect on androgen-dependent LNCaP prostate cancer cell line or normal human prostate epithelial PrEC cells, which was achieved by the induction of apoptosis in a mitochondrion-dependent intrinsic apoptotic pathway. Furthermore, Isorhamnetin inhibited cell migration and invasion in concentration-dependent manners by enhancing mesenchymal-epithelial transition (MET) and inhibiting matrix metalloproteinase (MMP) 2 (MMP-2) and MMP-9 overexpression. In addition, Isorhamnetin also down-regulated the expression of phosphorylated PI3K (p-P13K), Akt (p-Akt), and mTOR (p-mTOR) proteins in both cancer cells, revealing Isorhamnetin to be a selective PI3K-Akt-mTOR pathway inhibitor. In summary, these findings propose that Isorhamnetin might be a novel therapeutic candidate for the treatment of androgen-independent prostate cancer.


Biosci Rep


Bioscience reports


Cai F,Zhang Y,Li J,Huang S,Gao R




Has Abstract


2020-03-27 00:00:00












  • Diagnosis of nonsense mutations in Aspergillus nidulans.

    abstract::Three genotypically suppressible alleles, a1X4, alcA125, and niaD500, are phenotypically suppressed by aminoglycoside antibiotics. Unsuppressible alleles at these loci are unaffected as are known missense mutations at the yA and gdhA loci. This is consistent with the premise that the suppressible mutations are nonsens...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Martinelli SD,Roberts TJ

    更新日期:1983-01-01 00:00:00

  • Toxicity profiling of the ethanolic extract of Citrullus lanatus seed in rats: behavioral, biochemical and histopathological aspects.

    abstract::Citrullus lanatus (Cucurbitaceae) is conventionally used for the treatment of urinary tract infection, renal stones, hypertension, diabetes and diarrhoea. Current study evaluates acute and 28 days repeated toxicity ethanolic extract of C. lanatus seed (EECLS) in Wistar rats to measure its safety profile. The single do...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Belemkar S,Shendge PN

    更新日期:2021-01-29 00:00:00

  • Apoptosis dependent decrease of the intramembrane ion traffic in cultured mouse fibroblasts shown by conductivity dispersion.

    abstract::We have investigated the intramembranal ion traffic in apoptotic 3T6 cells in culture. Apoptosis was induced by various treatments, such as serum deprivation, high density growth and hydrogen peroxide at subnecrotic doses. Cell death was assessed by nucleosomal DNA fragmentation, single cell electrophoresis, immunoflu...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Bonincontro A,Iacoangeli A,Melucci-Vigo G,Risuleo G

    更新日期:1997-12-01 00:00:00

  • Osteogenic protein-1 attenuates apoptosis and enhances matrix synthesis of nucleus pulposus cells under high-magnitude compression though inhibiting the p38 MAPK pathway.

    abstract:BACKGROUND:Disc degeneration is correlated with mechanical load. Osteogenic protein-1 (OP-1) is potential to regenerate degenerative disc. OBJECTIVE:To investigate whether OP-1 can protect against high magitude compression-induced nucleus pulposus (NP) cell apoptosis and NP matrix catabolism, and its potential mechani...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Fang H,Li X,Shen H,Sun B,Teng H,Li P

    更新日期:2018-02-13 00:00:00

  • 6-(7-nitro-2,1,3-benzoxadiazol-4-ylthio) hexanol: a promising new anticancer compound.

    abstract::The 7-nitro-2,1,3-nitrobenzoxadiazole (NBD) derivatives are a series of compounds containing the NBD scaffold that are not glutathione (GSH) peptidomimetics, and result in a strong inhibition of glutathione S-transferases (GSTs). Growing evidences highlight their pivotal roles and outstanding anticancer activity in di...

    journal_title:Bioscience reports

    pub_type: 杂志文章,评审


    authors: Sha HH,Wang Z,Dong SC,Hu TM,Liu SW,Zhang JY,Wu Y,Ma R,Wu JZ,Chen D,Feng JF

    更新日期:2018-02-13 00:00:00

  • Modulation of the junctional integrity by low or high concentrations of cytochalasin B and dihydrocytochalasin B is associated with distinct changes in F-actin and ZO-1.

    abstract::In a study of Necturus gallbladder epithelium Benzel et al. (Benzel et al., 1980) found that low (0.2-1.2 microM) and higher concentrations (1.5 microM and more) of cytochalasin B (CB) caused an increase and decrease in the transepithelial electrical resistance (TER), respectively. Moreover, there were slight changes ...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Nybom P,Magnusson KE

    更新日期:1996-08-01 00:00:00

  • AMP hydrolysis in soluble and microsomal rat cardiac cell fractions: kinetic characterization and molecular identification of 5'-nucleotidase.

    abstract::The present study describes the enzymatic properties and molecular identification of 5'-nucleotidase in soluble and microsomal fractions from rat cardiac ventricles. Using AMP as a substrate, the results showed that the cation and the concentration required for maximal activity in the two fractions was magnesium at a ...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Pochmann D,Innocente AM,Cotomacci G,Barreto-Chaves ML,Sarkis JJ

    更新日期:2008-10-01 00:00:00

  • Comprehensive analysis of protein acetyltransferases of human pathogen Mycobacterium tuberculosis.

    abstract::Tuberculosis (TB), a leading infectious disease caused by Mycobacterium tuberculosis strain, takes four human lives every minute globally. Paucity of knowledge on M. tuberculosis virulence and antibiotic resistance is the major challenge for tuberculosis control. We have identified 47 acetyltransferases in the M. tube...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Xie L,Yang W,Fan X,Xie J

    更新日期:2019-12-20 00:00:00

  • Bacteria-host cell interaction mediated by cellular cholesterol/glycolipid-enriched microdomains.

    abstract::Gram negative bacterial infection is a leading cause of fatality and is attributed, at least in part, to the bacteria's capacity to persist in the host in spite of appropriate antibiotic therapy. It has been suggested that bacteria evade antibiotics by hiding within host cells. We sought to investigate this important ...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Shin JS,Gao Z,Abraham SN

    更新日期:1999-10-01 00:00:00

  • Inhibitory action of estradiol on growth promoting activity in extract from uterine cancers.

    abstract::Extracts from uterine cervical and body cancers, but not from benign tumor or intact tissues tested, were found to contain a growth-promoting activity which induced the proliferation of human endometrial fibroblasts. Exposure of cultured fibroblasts to the cancer extracts increased the rate of [3H]thymidine incorporat...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Imai A,Matsunami K,Iida K,Tamaya T

    更新日期:1990-02-01 00:00:00

  • Some properties of 2-5A binding/nucleolytic activities in gel filtered rabbit reticulocyte lysates.

    abstract::Rabbit reticulocyte lysates, gel filtered on Sephadex G-25 with or without ATP (or its analogs), were preincubated at 37 degrees C and their subsequent binding to p3A4,3'-[32P]pCp was studied. Lysates filtered without ATP or in the presence of 0.1 mM 8-bromo-ATP, 1,N6-etheno-ATP, or ITP showed a time-dependent decreas...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Wu JM,Wertheimer SJ,Eslami B,Figuereido JC,Goswami BB

    更新日期:1985-12-01 00:00:00

  • Rapid melanization of Bomirski amelanotic melanoma cells in cell culture.

    abstract::Transfer of Bomirski amelanotic melanoma cells from in vivo to in vitro growth conditions results in occurrence of rapid melanization in their cytoplasm. The melanized cells from primary cell culture initiate tumours in hamsters, which do not contain traces of melanin and resemble typical amelanotic melanoma. ...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Słomiński A

    更新日期:1983-02-01 00:00:00

  • Characterization of FANCL variants observed in patient cancer cells.

    abstract::Fanconi Anemia (FA) is a rare genetic disorder characterized by developmental defects, bone marrow failure and high predisposition to cancer. The FA DNA repair pathway is required in humans to coordinate repair of DNA interstrand cross-links. The central event in the activation of the pathway is the monoubiquitination...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Frost MG,Mazloumi Aboukheili AM,Toth R,Walden H

    更新日期:2020-06-26 00:00:00

  • Structure and ligand binding of As-p18, an extracellular fatty acid binding protein from the eggs of a parasitic nematode.

    abstract::Intracellular lipid-binding proteins (iLBPs) of the fatty acid-binding protein (FABP) family of animals transport, mainly fatty acids or retinoids, are confined to the cytosol and have highly similar 3D structures. In contrast, nematodes possess fatty acid-binding proteins (nemFABPs) that are secreted into the perivit...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Ibáñez-Shimabukuro M,Rey-Burusco MF,Gabrielsen M,Franchini GR,Riboldi-Tunnicliffe A,Roe AJ,Griffiths K,Cooper A,Córsico B,Kennedy MW,Smith BO

    更新日期:2019-07-23 00:00:00

  • Expression profile and prognostic value of SFN in human ovarian cancer.

    abstract::Ovarian cancer is a highly lethal cancer in females. Therefore, it is necessary to explore effective biomarkers for the diagnosis and prognosis of the disease. Stratifin (SFN) is a cell cycle checkpoint protein that has been reported to be involved in oncogenesis. Our studies detected the expression of SFN in ovarian ...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Hu Y,Zeng Q,Li C,Xie Y

    更新日期:2019-05-02 00:00:00

  • LINC00673 rs11655237 C>T confers neuroblastoma susceptibility in Chinese population.

    abstract::Neuroblastoma, which accounts for approximately 10% of all pediatric cancer-related deaths, has become a therapeutic challenge and global burden attributed to poor outcomes and mortality rates of its high-risk form. Previous genome-wide association studies (GWASs) identified the LINC00673 rs11655237 C>T polymorphism t...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Zhang Z,Chang Y,Jia W,Zhang J,Zhang R,Zhu J,Yang T,Xia H,Zou Y,He J

    更新日期:2018-02-08 00:00:00

  • Aluminium-induced bone disease in uremic rats: effect of deferoxamine.

    abstract::We have previously established a rat model of chronic uremia, which is suitable to investigate the effect of various treatment modalities on renal osteodystrophy [1]. After four months subsequent to 5/6 nephrectomy, some animals were treated by gavage for 9 weeks with tap water (controls), or with aluminium (Al-citrat...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Jablonski G,Klem KH,Danielsen CC,Mosekilde L,Gordeladze JO

    更新日期:1996-02-01 00:00:00

  • Prevalence and type distribution of human papillomavirus (HPV) in Malaysian women with and without cervical cancer: an updated estimate.

    abstract::Information on the prevalence and type distribution of human papillomavirus (HPV) among Malaysian women is currently limited. The present study therefore aimed to provide an updated estimate on the prevalence and type distribution of HPV among Malaysian women with and without cervical cancer. Total DNA was isolated fr...

    journal_title:Bioscience reports

    pub_type: 临床试验,杂志文章


    authors: Tan SC,Ismail MP,Duski DR,Othman NH,Ankathil R

    更新日期:2018-03-29 00:00:00

  • The role of transmembrane sodium gradient in rabbit ileal smooth muscle: utilization of harmaline.

    abstract::Decreasing extracellular sodium concentration was found to produce a contractile response of rabbit ileal smooth muscle. As the concentration decreases, the amplitude of contraction increases, thus producing a dose-dependent curve. Harmaline, a competitor for sodium, was found to inhibit the sodium gradient-dependent ...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Suleiman MS

    更新日期:1985-08-01 00:00:00

  • Higher Ki67 expression in fibroblast like cells at invasive front indicates better clinical outcomes in oral squamous cell carcinoma patients.

    abstract::Background: Ki67 has been a key role for the treatment options and prognosis evaluation in some kinds of tumors; however, the spatial expression of Ki67 in oral squamous cell carcinoma (OSCC) has not been fully-evaluated. Therefore, in the present study, we aimed to elucidate the prognosis value of Ki67 spatial expres...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Jing Y,Yang Y,Hao F,Song Y,Zhang X,Zhang Y,Huang X,Hu Q,Ni Y

    更新日期:2018-11-20 00:00:00

  • Hydrogen sulfide attenuates cardiac dysfunction in a rat model of heart failure: a mechanism through cardiac mitochondrial protection.

    abstract::HF (heart failure) after MI (myocardial infarction) is a major cause of morbidity and mortality worldwide. Recent studies have shown that hydrogen sulfide (H2S) has cardioprotective effects. Hence, we aimed to elucidate the potential effects of H2S on HF after MI in rats. The HF model after MI was made by ligating the...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Wang X,Wang Q,Guo W,Zhu YZ

    更新日期:2011-04-01 00:00:00

  • Adrenaline reactivation of muscle phosphorylase after deactivation during phasic contractile activity.

    abstract::The increase in % phosphorylase a associated with muscle contraction is reversed after a few minutes despite continued contractile activity. The present study was undertaken to determine whether adrenaline can activate phosphorylase after deactivation has occurred during prolonged stimulation of contraction. We found ...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Rennie MJ,Fell RD,Ivy JL,Holloszy JO

    更新日期:1982-05-01 00:00:00

  • miRNA-148b suppresses hepatic cancer stem cell by targeting neuropilin-1.

    abstract::The existence of cancer stem cells (CSCs) is considered as a direct reason for the failure of clinic treatment in hepatocellular carcinoma (HCC). Growing evidences have demonstrated that miRNAs play an important role in regulation of stem cell proliferation, differentiation and self-renewal and their aberrances cause ...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Liu Q,Xu Y,Wei S,Gao W,Chen L,Zhou T,Wang Z,Ying M,Zheng Q

    更新日期:2015-05-22 00:00:00

  • A low-molecular-weight RNA species in yeast mitochondria arising from a 3' end trimming of cytochrome b pre-mRNA.

    abstract::Immobilization of yeast mitochondrial RNA on nitrocellulose filters without prior alkali treatment revealed a low-MW RNA species (integral of 300 bases) which hybridizes specifically to the RNA coding strand of a DNA fragment BglII-HinfI at the 3' end of the COB-BOX gene. This RNA species (often a doublet) was found i...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Halbreich A,Grandchamp C,Foucher M

    更新日期:1981-07-01 00:00:00

  • Increase of glandular epithelial cell clusters by an external volume expansion device promotes adipose tissue regeneration by recruiting macrophages.

    abstract::Background: There is a clinical need for the use of engineered adipose tissue in place of surgical reconstruction. We previously found that the external volume expansion (EVE) device increased special cell clusters in well-vascularized connective stroma during adipose regeneration. However, the origin of these cell cl...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Chen X,He Y,Xu A,Deng Z,Feng J,Lu F,Yuan Y

    更新日期:2019-02-26 00:00:00

  • Comprehensive analysis of prognostic biomarkers in lung adenocarcinoma based on aberrant lncRNA-miRNA-mRNA networks and Cox regression models.

    abstract::Lung adenocarcinoma (LUAD) is the leading cause of cancer-related death worldwide, and its underlying mechanism remains unclear. Accumulating evidence has highlighted that long non-coding RNA (lncRNA) acts as competitive endogenous RNA (ceRNA) and plays an important role in the occurrence and development of LUAD. Here...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Yao Y,Zhang T,Qi L,Liu R,Liu G,Wang J,Song Q,Sun C

    更新日期:2020-01-31 00:00:00

  • PU.1-deficient mice are resistant to thioacetamide-induced hepatic fibrosis: PU.1 finely regulates Sirt1 expression via transcriptional promotion of miR-34a and miR-29c in hepatic stellate cells.

    abstract::PU box binding protein (PU.1) is a critical transcription factor involved in many pathological processes. However, its exact role in activation of hepatic stellate cells (HSCs) and liver fibrosis was rarely reported. Here, we found that, in HSCs of PU.1+/- mice, Sirt1 mRNA expression was not changed but Sirt1 protein ...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Liu Q,Zhang Y,Yang S,Wu Y,Wang J,Yu W,Liu Y

    更新日期:2017-12-12 00:00:00

  • Phytolacca americana lectin (Pa-2; pokeweed mitogen): an intrinsically unordered protein and its conversion into partial order at low pH.

    abstract::This is the first report of its kind that well demonstrates that a lectin from Phytolacca americana [Pa-2 (P. americana lectin-2)] can also be intrinsically unordered, based on the results obtained by CD, tryptophan fluorescence, ANS (8-anilinonaphthalene-1-sulfonic acid) binding, acrylamide quenching, DLS (dynamic li...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Ahmad E,Kamranur Rahman S,Masood Khan J,Varshney A,Hasan Khan R

    更新日期:2009-11-23 00:00:00

  • The effect of tunicamycin on transferrin synthesis and secretion in hormonally stimulated explants of rabbit mammary gland.

    abstract::Pregnant rabbit mammary gland explants cultured with insulin, prolactin and cortisol synthesize and secrete transferrin radiolabelled with [3H]leucine or [3H]mannose. Addition of tunicamycin inhibits the glycosylation but not synthesis of the [3H]leucine-labelled polypeptide. Secretion of the non-glycosylated protein ...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Bradshaw JP,White DA

    更新日期:1985-03-01 00:00:00

  • Genetic and structural validation of Aspergillus fumigatus N-acetylphosphoglucosamine mutase as an antifungal target.

    abstract::Aspergillus fumigatus is the causative agent of IA (invasive aspergillosis) in immunocompromised patients. It possesses a cell wall composed of chitin, glucan and galactomannan, polymeric carbohydrates synthesized by processive glycosyltransferases from intracellular sugar nucleotide donors. Here we demonstrate that A...

    journal_title:Bioscience reports

    pub_type: 杂志文章


    authors: Fang W,Du T,Raimi OG,Hurtado-Guerrero R,Mariño K,Ibrahim AF,Albarbarawi O,Ferguson MA,Jin C,Van Aalten DM

    更新日期:2013-09-04 00:00:00