Abstract：
:Measles virus and mumps virus (MeV and MuV) are enveloped RNA viruses used for production of live attenuated vaccines for prophylaxis of measles and mumps disease, respectively. For biotechnological production of and basic research on these viruses, the preparation of highly purified and infectious viruses is a prerequisite, and to meet that aim, knowledge of their stability and biophysical properties is crucial. Our goal was to carry out a detailed investigation of the stability of MeV and MuV under various pH, temperature, shear stress, filtration and storage conditions, as well as to evaluate two commonly used purification techniques, ultracentrifugation and diafiltration, with regard to their efficiency and effect on virus properties. Virus titers were estimated by CCID50 assay, particle size and concentration were measured by Nanoparticle tracking analysis (NTA) measurements, and the host cell protein content was determined by ELISA. The results demonstrated the stability of MuV and MeV at pH <9 and above pH 4 and 5, respectively, and aggregation was observed at pH >9. Storage without stabilizer did not result in structural changes, but the reduction in infectivity after 24 hours was significant at +37 °C. Vortexing of the viruses resulted in significant particle degradation, leading to lower virus titers, whereas pipetting had much less impact on virus viability. Diafiltration resulted in higher recovery of both total and infectious virus particles than ultracentrifugation. These results provide important data for research on all upstream and downstream processes on these two viruses regarding biotechnological production and basic research.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Sviben D,Forčić D,Kurtović T,Halassy B,Brgles Mdoi
10.1007/s00705-016-2801-3subject
Has Abstractpub_date
2016-06-01 00:00:00pages
1455-67issue
6eissn
0304-8608issn
1432-8798pii
10.1007/s00705-016-2801-3journal_volume
161pub_type
杂志文章abstract：:A rotavirus-like virus has been isolated from cases of neonatal diarrhoea in piglets. No antigenic relationship with known rotaviruses or with an American strain of a rotavirus-like virus has been demonstrated. Morphologically the virus is similar to known rotaviruses, but it differs in the ability to form syncytia of...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311220
更新日期：1984-01-01 00:00:00
abstract：:Noroviruses belong to a genus of genetically diverse viruses within the family Caliciviridae and cause acute gastroenteritis in humans and animals. They are subdivided into genogroups, each of which further segregates into genotypes. Until recently, a new genotype was based on a defined pairwise distance cutoff of com...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1708-5
更新日期：2013-10-01 00:00:00
abstract：:A new member of the genus Nepovirus was isolated from blueberry in Japan. The virus was associated with latent infection of blueberry trees and provisionally named blueberry latent spherical virus (BLSV). BLSV was found to have isometric particles approximately 30 nm in diameter, which were composed of a single coat p...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1177-7
更新日期：2012-02-01 00:00:00
abstract：:The present work shows that at least four different sequence types of Aleutian mink disease parvovirus (ADV) are present in ADV isolates from mink. We here report the nucleotide sequences of these four types of ADV from nucleotide 123 to 2208 (map unit 3 to 46). This part of the genome encodes three non-structural (NS...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01379127
更新日期：1994-01-01 00:00:00
abstract：:We present the molecular characterization of a new virus infecting yerba mate (Ilex paraguariensis St. Hil.) in Argentina. Deep sequencing of diseased yerba mate plants showing chlorotic linear patterns, chlorotic rings, and vein yellowing resulted in the identification of a new virus resembling plant rhabdoviruses in...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3363-8
更新日期：2017-08-01 00:00:00
abstract：:The base sequences of the coding region of the nucleoprotein (NP) genes of two H 10 influenza A viruses, one avian (virus N) and one mink virus, have been determined by primer extension. When the NP genes and the NP sequences derived from the only open reading frame of the two H 10 viruses were compared with those of ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01319816
更新日期：1988-01-01 00:00:00
abstract：:Cell cultures inoculated with caprine respiratory syncytial virus (RSV) were studied with light, fluorescent, and electron microscopy to determine the morphogenesis and structure of the virus. Small syncytia were seen after 36 hours in culture. After 48 hours in culture, syncytia were large and numerous and pleomorphi...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314543
更新日期：1980-01-01 00:00:00
abstract：:The present study confirms that herpesviruses (LF) previously isolated from testes and buffy coat cells of male turkeys with semen abnormalities establish a latent infection in testicular cells. These experiments also present the first evidence that these herpesviruses are harbored latently in cells of spinal ganglia....
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311163
更新日期：1984-01-01 00:00:00
abstract：:We constructed a recombinant baculovirus encoding a dengue (DEN)-2 virus envelope glycoprotein truncated of 102 amino acids (aa) at its C-terminus (D2E delta 102). The production, processing and transportation of the recombinant protein in baculovirus-infected Spodoptera frugiperda (Sf9) cells and its immunogenic prop...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309465
更新日期：1994-01-01 00:00:00
abstract：:Bovine leukemia virus (BLV) causes enzootic bovine leucosis (EBL) and is responsible for substantial economic losses in cattle globally. However, information in Africa on the disease is limited. Here, based on clinical, hematological, pathological and molecular analyses, two clinical cases of EBL were confirmed in a d...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3205-0
更新日期：2017-04-01 00:00:00
abstract：:Phylogenetic relationships of novel Vietnamese strains of feline immunodeficiency virus (FIV) were analysed. One Vietnamese strain was found to cluster with subtype D, which was previously known only in Japan, while the other seven strains were placed with members of subtype C. Calculation of the relative numbers of m...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0954-8
更新日期：2003-04-01 00:00:00
abstract：:Serological and genetical relationships of three salmonid herpesviruses [strain H-83, OO-7812 strain of Oncorhynchus masou virus (OMV) and Herpesvirus salmonis] were investigated. Although the serological relationships of these viruses were not distinctly demonstrated by cross-neutralization test, cross enzyme-linked ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313818
更新日期：1989-01-01 00:00:00
abstract：:The effect of temperature on the response of tomato (Lycopersicon esculentum Mill. cv. Rutgers) to infections with the necrogenic cucumber mosaic virus (CMV) satellite D-CARNA 5 was investigated with each of four CMV strains D, 1, Y and S functioning as helper virus. At 24 degrees C lethal necrosis was observed in all...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309724
更新日期：1995-01-01 00:00:00
abstract：:The CPK cells derived from swine kidney were infected with the attenuated TO-163 strain of transmissible gastroenteritis (TGE) virus, and fused with uninfected Vero cells in the presence of polyethylene glycol. Repeated cocultivation of the fused cells with uninfected Vero cells rendered the virus to grow in Vero cell...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01321128
更新日期：1992-01-01 00:00:00
abstract：:Avian influenza virus infections are a major cause of morbidity and rapid identification of the virus has important clinical, economical and epidemiological implications. We have developed a one-tube Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) for the rapid diagnosis of avian influenza A. A panel of refer...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170193
更新日期：2001-01-01 00:00:00
abstract：:Nanoviruses and geminiviruses are circular, single stranded DNA viruses that infect many plant species around the world. Nanoviruses and certain geminiviruses that belong to the Begomovirus and Mastrevirus genera are associated with additional circular, single stranded DNA molecules (~ 1-1.4 kb) that encode a replicat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3854-2
更新日期：2018-09-01 00:00:00
abstract：:We have sequenced the coxsackievirus A 21 polymerase gene and 3' noncoding region. The sequence is 98 per cent homologous at the amino acid level to the three poliovirus serotypes. This is comparable to the relationship between polioviruses and indicates a recent evolutionary divergence of the viruses. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313732
更新日期：1987-01-01 00:00:00
abstract：:To determine the prevalence of enteroviruses in aquatic environments in Shenzhen, water samples were collected monthly from April 2010 to December 2011. After concentration by the filter adsorption-beef extract elution method, the enteroviruses were propagated in RD cells. Thirty-five of 105 water samples (33.33 %) we...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1611-0
更新日期：2013-06-01 00:00:00
abstract：:The complete genome of O/Akesu/58 strain of foot-and-mouth disease virus (FMDV) was sequenced. The phylogenetic analysis revealed that it is not closely related to epidemic strains or previous strains compared with reference sequences (the identities of complete VP1 nucleotide sequences range from 77.5 to 84.0%). Its ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-1041-y
更新日期：2007-01-01 00:00:00
abstract：:Mutations were introduced by recombinant DNA techniques into 9 genes of an infectious molecular clone of human immunodeficiency virus type 1. The 24 mutants generated were characterized biochemically and biologically by transfection and infection experiments. None of the mutants which have mutations in gag (p17, p24, ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310491
更新日期：1991-01-01 00:00:00
abstract：:The EDS adenovirus produced either in the allantoic cavity of embryonated duck eggs (A0 virus) or in chicken embryo liver cell cultures (TC0 virus) were comparatively studied by gradient ultracentrifugation in CsCl. The A0 viral particles banded at densities of 1.36 and 1.31 g/ml, whereas, the TC0 viral particle were ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314574
更新日期：1981-01-01 00:00:00
abstract：:This report presents a molecular characterization of the complete genome of a rare hepatitis C virus (HCV) genotype (GT5a) from India. Sequence homology of full genome revealed that the strain belonged to HCV GT5a. To trace the origin of this virus and to understand its evolutionary pattern, a phylogenetic reconstruct...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2220-2
更新日期：2015-01-01 00:00:00
abstract：:Here we describe the results of experimental infections of captive big brown bats (Eptesicus fuscus) with three newly isolated bat lyssaviruses from Eurasia (Aravan, Khujand, and Irkut viruses). Infection of E. fuscus was moderate (total, 55-75%). There was no evidence of transmission to in-contact cage mates. Incubat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0785-0
更新日期：2006-10-01 00:00:00
abstract：:Rabies is one of the most important zoonotic diseases and is caused by several rabies virus (RABV) variants. These variants can exhibit differences in neurovirulence, and few studies have attempted to evaluate the neuroinvasiveness of variants derived from vampire bats and wild carnivores. The aim of this study was to...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3530-y
更新日期：2017-12-01 00:00:00
abstract：:The numbers of the carbohydrate side chains have been analyzed on a series of subtype 7 hemagglutinins of influenza A virus. The procedure employed involves growth of the virus in the presence of the trimming inhibitor N-methyl-1-desoxynojirimycin followed by controlled removal of the resulting mannose-rich oligosacch...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314433
更新日期：1987-01-01 00:00:00
abstract：:Aphidicolin, a specific inhibitor of cellular DNA polymerase alpha and of viral DNA polymerase, inhibits production of infectious virus and cellular and viral DNA synthesis of human cytomegalovirus (HCMV)-infected cells. On the other hand, 2',3'-dideoxynucleosides, inhibitors of DNA polymerases beta and gamma, do not ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310559
更新日期：1985-01-01 00:00:00
abstract：:The high prevalence (14.3%) of HIV/HBV co-infections reported in west China makes it necessary to screen concurrent HBV infection in HIV carriers. HBV B genotype was shown to be dominant in 54 cases of HIV/HBV co-infection, accounting for 81.48% of the total. The total drug resistance rate observed was 3.70%. A1762T, ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3900-0
更新日期：2018-10-01 00:00:00
abstract：:"Phages" include viruses of eubacteria and archaea. At least 5568 phages have been examined in the electron microscope since the introduction of negative staining in 1959. Most virions (96%) are tailed. Only 208 phages (3.7%) are polyhedral, filamentous, or pleomorphic. Phages belong to one order, 17 families, and thr...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-006-0849-1
更新日期：2007-02-01 00:00:00
abstract：:An archival brain sample from an infected dog taken during the 1988/89 rabies outbreak in Finland was investigated to confirm that it was infected with classical rabies virus (genotype 1). The first 400 base pairs of the virus nucleoprotein gene were then sequenced and used to undertake a phylogenetic comparison betwe...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0523-z
更新日期：2005-07-01 00:00:00
abstract：:Gangliosides from different cells were tested for their inhibiting activity on VSV attachment to CER cells and goose erythrocytes. This inhibiting activity was enhanced when gangliosides were inserted into liposomes, in particular if containing phosphatidylserine. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310981
更新日期：1987-01-01 00:00:00
