Abstract：
BACKGROUND:Ethanol and cocaine are widely abused drugs triggering long-lasting changes in neuronal circuits and synaptic transmission through the regulation of enzyme activity and gene expression. Compelling evidence indicates that the ubiquitin-proteasome system plays a role in the molecular changes induced by addictive substances, impacting on several mechanisms implicated in abuse. The goal of these studies was to evaluate the effects of cocaine or ethanol on proteasome activity in neuroblastoma cells. Moreover, the gene expression of specific subunits was assessed. METHODS:Chymotrypsin-like activity was measured after 2 h, 24 h, and 48 h exposure to 5 μM cocaine or 40 mM ethanol. Proteasome subunit transcripts were evaluated by qPCR at the same time-points. RESULTS:Treatments modified proteasome function in opposite directions, since cocaine increased and ethanol reduced chymotrypsin-like activity. Interestingly, we observed gene expression alterations induced by these drugs. In the core particle, the β1 and α5 subunits were mainly up-regulated by cocaine, whereas α6 transcripts were mostly decreased. β2 and β5 did not change. Similarly, ethanol exposure generally increased β1 and α5 mRNAs. Moreover, the β2 subunit was significantly up-regulated by ethanol only. The β5 and α6 subunits were not altered. In the regulatory particle, Rpt3 was increased by cocaine exposure, whereas it was reduced by ethanol. No significant Rpn9 alterations were observed. CONCLUSIONS:These findings support the notion that addictive substances regulate proteasome function, contributing to the dysregulations related to drug abuse since the availability of adequate subunit amounts is necessary for proper complex assembly and function.
journal_name
Drug Alcohol Dependjournal_title
Drug and alcohol dependenceauthors
Caputi FF,Carboni L,Mazza D,Candeletti S,Romualdi Pdoi
10.1016/j.drugalcdep.2016.02.012subject
Has Abstractpub_date
2016-04-01 00:00:00pages
265-75eissn
0376-8716issn
1879-0046pii
S0376-8716(16)00087-9journal_volume
161pub_type
杂志文章abstract：:The Subjective Opiate Withdrawal Questionnaire (SOWQ) and the Objective Opiate Withdrawal Scale (OOWS) were administered to detect withdrawal distress during a rapid opiate detoxification. No correlations could be observed between the clinical assessments and self ratings of the 20 patients participating in this study...
journal_title：Drug and alcohol dependence
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journal_title：Drug and alcohol dependence
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journal_title：Drug and alcohol dependence
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journal_title：Drug and alcohol dependence
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journal_title：Drug and alcohol dependence
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journal_title：Drug and alcohol dependence
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更新日期：1983-10-01 00:00:00
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journal_title：Drug and alcohol dependence
pub_type： 临床试验,杂志文章,随机对照试验
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journal_title：Drug and alcohol dependence
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journal_title：Drug and alcohol dependence
pub_type： 临床试验,杂志文章,随机对照试验
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更新日期：1990-11-01 00:00:00
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journal_title：Drug and alcohol dependence
pub_type： 杂志文章
doi：10.1016/j.drugalcdep.2013.11.020
更新日期：2014-02-01 00:00:00
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journal_title：Drug and alcohol dependence
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journal_title：Drug and alcohol dependence
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journal_title：Drug and alcohol dependence
pub_type： 杂志文章
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journal_title：Drug and alcohol dependence
pub_type： 临床试验,杂志文章
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更新日期：1996-09-01 00:00:00
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journal_title：Drug and alcohol dependence
pub_type： 杂志文章
doi：10.1016/j.drugalcdep.2017.12.024
更新日期：2018-04-01 00:00:00
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journal_title：Drug and alcohol dependence
pub_type： 杂志文章,随机对照试验
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更新日期：2009-09-01 00:00:00
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journal_title：Drug and alcohol dependence
pub_type： 杂志文章
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更新日期：2020-04-01 00:00:00
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journal_title：Drug and alcohol dependence
pub_type： 杂志文章
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更新日期：2020-03-01 00:00:00
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journal_title：Drug and alcohol dependence
pub_type： 杂志文章
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更新日期：2020-04-01 00:00:00
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journal_title：Drug and alcohol dependence
pub_type： 杂志文章
doi：10.1016/j.drugalcdep.2012.06.019
更新日期：2013-01-01 00:00:00
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journal_title：Drug and alcohol dependence
pub_type： 杂志文章
doi：10.1016/j.drugalcdep.2009.08.002
更新日期：2010-01-15 00:00:00
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journal_title：Drug and alcohol dependence
pub_type： 杂志文章
doi：10.1016/j.drugalcdep.2013.05.027
更新日期：2013-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2008-06-01 00:00:00
