Effects of esculetin on lipopolysaccharide (LPS)-induced acute lung injury via regulation of RhoA/Rho Kinase/NF-кB pathways in vivo and in vitro.


:The purpose of the present study was to investigate the protective effect of esculetin (ES) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the lung epithelial A549 cells. Mice were intragastrically administered with ES (20 and 40 mg/kg) 1 h prior to LPS challenge. ES pretreatment at doses of 20 and 40 mg/kg effectively attenuated LPS-induced lung histopathological change, myeloperoxidase or MPO activity, inflammatory cells infiltration, pulmonary wet-to-dry weight ratio, and the generation of pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in vivo and in vitro. Furthermore, we demonstrated that ES blocked the activation of NF-кB and RhoA/Rho kinase pathways in LPS-induced mice and A549 cells. The results suggested that ES exhibited protective effect on ALI and might attribute partly to the inhibition of NF-кB and RhoA/Rho kinase pathways in vivo and in vitro.


Free Radic Res


Free radical research


Chen T,Guo Q,Wang H,Zhang H,Wang C,Zhang P,Meng S,Li Y,Ji H,Yan T




Has Abstract


2015-01-01 00:00:00












  • Oxidative inactivation of the proteasome in Alzheimer's disease.

    abstract::In the present study we isolated proteasome complexes from control, mild cognitive impairment (MCI), and Alzheimer's disease (AD) subjects. No significant difference in the amount of proteasomes was detected across the different groups, although impairments in chymotrypsin-like proteasome activity was observed in AD s...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Cecarini V,Ding Q,Keller JN

    更新日期:2007-06-01 00:00:00

  • Posttranslational protein modifications by reactive nitrogen and chlorine species and strategies for their prevention and elimination.

    abstract::Proteins are subject to various posttranslational modifications, some of them being undesired from the point of view of metabolic efficiency. Prevention of such modifications is expected to provide new means of therapy of diseases and decelerate the process of aging. In this review, modifications of proteins by reacti...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Sadowska-Bartosz I,Ott C,Grune T,Bartosz G

    更新日期:2014-11-01 00:00:00

  • Neurofilaments are the major neuronal target of hydroxynonenal-mediated protein cross-links.

    abstract::Lipid peroxidation generates reactive aldehydes, most notably hydroxynonenal (HNE), which covalently binds amino acid residue side chains leading to protein inactivation and insolubility. Specific adducts of lipid peroxidation have been demonstrated to be intimately associated with pathological lesions of Alzheimer's ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Perry EA,Castellani RJ,Moreira PI,Nunomura A,Lui Q,Harris PL,Sayre LM,Szweda PA,Szweda LI,Zhu X,Smith MA,Perry G

    更新日期:2013-07-01 00:00:00

  • Prevention of intracellular oxidative stress to lens by pyruvate and its ester.

    abstract::Pyruvate is a well-known scavenger of hydrogen peroxide (H2O2). In addition, it scavenges superoxide radical (O2.-). However, evidence on its intracellular antioxidant function is meager at present. Hence, we have examined the effectiveness of this metabolite and its ethyl ester against intracellular oxidative damage ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Varma SD,Devamanoharan PS,Ali AH

    更新日期:1998-02-01 00:00:00

  • Melatonin nitrosation promoted by NO*2; comparison with the peroxynitrite reaction.

    abstract::N-nitroso species have recently been detected in animal tissues. Protein N-nitrosotryptophan is the best candidate for this N-nitroso pool. N-nitrosation of N-blocked trytophan derivatives like melatonin (MelH) by N2O3 or peroxynitrite (ONOOH/ONOO- ) has been observed under conditions of pH and reagent concentrations ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Peyrot F,Houée-Levin C,Ducrocq C

    更新日期:2006-09-01 00:00:00

  • ABC transporters affect the detection of intracellular oxidants by fluorescent probes.

    abstract::Intracellular production of reactive oxygen species (ROS) plays an important role in the control of cell physiology. For the assessment of intracellular ROS production, a plethora of fluorescent probes is commonly used. Interestingly, chemical structures of these probes imply they could be substrates of plasma membran...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Procházková J,Kubala L,Kotasová H,Gudernová I,Šrámková Z,Pekarová M,Sarkadi B,Pacherník J

    更新日期:2011-07-01 00:00:00

  • Late-intervention study with ebselen in an experimental model of type 1 diabetic nephropathy.

    abstract:BACKGROUND AND AIM:Previous studies have shown that preventive treatment with the antioxidant, ebselen, in experimental models of type 1 diabetic nephropathy resulted in an attenuation of structural and functional damage in the kidney. However, evidence for the effectiveness of ebselen in late-intervention studies is l...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Tan SM,Sharma A,Stefanovic N,de Haan JB

    更新日期:2015-03-01 00:00:00

  • Metallothionein over-expression in podocytes reduces adriamycin nephrotoxicity.

    abstract::Adriamycin (ADR) is nephrotoxic. One component of ADR-induced nephropathy may be oxidative stress. This study used a recently developed line of transgenic mice (Nmt) on the FVB background strain, which over-express the antioxidant protein metallothionein (MT) in podocytes. Cultured podocytes from Nmt mice were resista...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Yang L,Zheng S,Epstein PN

    更新日期:2009-02-01 00:00:00

  • Short term citrus flavonoid supplementation of type II diabetic women: no effect on lipoprotein oxidation tendencies.

    abstract::Flavonoids, which are dietary components and have possible drug uses, inhibit lipoprotein oxidation in vitro. The present study considered whether flavonoid supplementation in humans could influence lipoprotein vulnerability to oxidation. Citrus flavonoid supplementation (about 1g/day, 3 weeks), or placebo, was given ...

    journal_title:Free radical research

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Blostein-Fujii A,DiSilvestro RA,Frid D,Katz C

    更新日期:1999-04-01 00:00:00

  • Investigation on the antioxidation of the flavonoids based on the photoinduced chemiluminescence of lucigenin by a new Zinc(II) phthalocyanine compound.

    abstract::A zinc(II) phthalocyanine compound, tetra-alpha-(2,2,4-tirmethyl-3-pentoxy) Phthalocyanine Zinc (ZnPc(OR)(4)) was synthesized in this paper and this zinc (II) phthalocyanine compound was used as the photosensitizer in the photoinduced chemiluminescence (PCL) of lucigenin in N,N-dimethylformamide (DMF). The photoexcite...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Wang J,Jiang Z,Huang L,Huang J,Chen G

    更新日期:2008-02-01 00:00:00

  • A validated method for rapid analysis of ethane in breath and its application in kinetic studies in human volunteers.

    abstract::Oxidative stress may initiate lipid peroxidation that generates ethane. Ethane, at low concentrations, is eliminated by pulmonary exhalation. Previous methods have not allowed frequent sampling, thus ethane kinetics has not been studied in man. A validated method over the range 3.8-100,000 ppb with a limit of quantita...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Dale O,Bergum H,Lund T,Nilsen T,Aadahl P,Stenseth R

    更新日期:2003-08-01 00:00:00

  • Qualitative analysis of phospholipids and their oxidised derivatives - used techniques and examples of their applications related to lipidomic research and food analysis.

    abstract::Phospholipids (PLs) are important biomolecules that not only constitute structural building blocks and scaffolds of cell and organelle membranes but also play a vital role in cell biochemistry and physiology. Moreover, dietary exogenous PLs are characterised by high nutritional value and other beneficial health effect...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Parchem K,Sasson S,Ferreri C,Bartoszek A

    更新日期:2019-01-01 00:00:00

  • Plasma protein carbonyls in nonpregnant, healthy pregnant and preeclamptic women.

    abstract::Increased reactive oxygen species (ROS) and lipid peroxidation may be implicated in the pathogenesis of preeclampsia by causing cell (membrane) damage and impaired endothelial function. Carbonyl derivatives of proteins, or protein carbonyls, may be sensitive biomarkers of ROS-mediated damage. The aim of the study was ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Zusterzeel PL,Mulder TP,Peters WH,Wiseman SA,Steegers EA

    更新日期:2000-11-01 00:00:00

  • Antioxidants inhibit the human cortical neuron apoptosis induced by hydrogen peroxide, tumor necrosis factor alpha, dopamine and beta-amyloid peptide 1-42.

    abstract::Several substances related to the neurodegenerative diseases of Alzheimer and Parkinson, such as hydrogen peroxide, tumor necrosis factor alpha, dopamine and beta-amyloid peptide 1-42, have been shown to induce apoptosis in tumoral cell lines and rat neurons but not in human neurons. Moreover, the role of mitochondria...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Medina S,Martínez M,Hernanz A

    更新日期:2002-11-01 00:00:00

  • BSC2 enhances cell resistance to AmB by inhibiting oxidative damage in Saccharomyces cerevisiae.

    abstract::Amphotericin B has been the gold standard for the treatment of invasive mycosis for many years. Its resistance mechanisms are reported to be mainly related to the decrease of ergosterol content or the changes of cell wall. Previous study has shown that Saccharomyces cerevisiae strain lack of BSC2 was sensitive signifi...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Kong Y,Wang Q,Cao F,Zhang X,Fang Z,Shi P,Wang H,Shen Y,Huang Z

    更新日期:2020-04-01 00:00:00

  • HO-1 mitigates acute kidney injury and subsequent kidney-lung cross-talk.

    abstract::Ischemia-reperfusion injury (IRI) is a leading cause of acute kidney injury (AKI), which contributes to the development of chronic kidney disease (CKD). IRI-induced AKI releases proinflammatory cytokines (e.g. IL-1β, TNF-α, IL-6) that induce a systemic inflammatory response, resulting in proinflammatory cells recruitm...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Rossi M,Delbauve S,Roumeguère T,Wespes E,Leo O,Flamand V,Le Moine A,Hougardy JM

    更新日期:2019-10-01 00:00:00

  • Astragaloside IV synergizes with ferulic acid to suppress hepatic stellate cells activation in vitro.

    abstract::Because hepatic fibrosis usually involves more than one pathological process, combination therapy with modalities that target aberrant signaling cascade in activated hepatic stellate cells (HSCs) represents an alternative strategy. This study evaluates the hypothesis that astragaloside IV (AS-IV) and ferulic acid (FA)...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Dong H,Guo H,Liang Y,Wang X,Niu Y

    更新日期:2017-02-01 00:00:00

  • Scutellarin ameliorates nonalcoholic fatty liver disease through the PPARγ/PGC-1α-Nrf2 pathway.

    abstract::Nonalcoholic fatty liver disease (NAFLD) is characterised by excessive accumulation of hepatic lipids and oxidative injury of hepatocytes. Scutellarin is a flavonoid glycoside having antioxidative stress activity. Our current study aims to investigate the molecular mechanism of scutellarin ameliorating NAFLD. Scutella...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Zhang X,Ji R,Sun H,Peng J,Ma X,Wang C,Fu Y,Bao L,Jin Y

    更新日期:2018-02-01 00:00:00

  • Plasma reactive carbonyl species: Potential risk factor for hypertension.

    abstract::To study the role of oxidative stress in hypertension and pre-hypertension, this study analysed plasma levels of reactive carbonyl species (RCS) in 1204 Chinese Han adults. Results showed a statistically significant positive correlation (p < 0.001) between blood pressure and plasma RCS levels with or without being adj...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Chen K,Xie F,Liu S,Li G,Chen Y,Shi W,Hu H,Liu L,Yin D

    更新日期:2011-05-01 00:00:00

  • Free radical DNA adduct 8-OH-deoxyguanosine affects activity of Hpa II and Msp I restriction endonucleases.

    abstract::8-OH-deoxyguanosine can diminish the ability of the restriction endonucleases Hpa II and Msp I to cleave DNA. The exact position of the adduct within the recognition site appears to determine the extent of the effect. ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Turk PW,Weitzman SA

    更新日期:1995-09-01 00:00:00

  • Validation of markers for their relevance to health and disease.

    abstract::Validation of markers requires two different steps. The first one is the analytical validation including analytical procedures, definition of standard reference, and round-tests analysis. The second one is the assessment of the biological relevance of the selected marker. This biological relevance may be in the field ...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Antoine JM,Diplock AT

    更新日期:2000-11-01 00:00:00

  • Absence of synproportionation between oxy and ferryl leghemoglobin. off.

    abstract::The synproportionation reaction between ferryl leghemoglobin and oxyleghemoglobin does not occur, at least under conditions where this process could be clearly demonstrated with myoglobin and hemoglobin. In contrast, a cross synproportionation can occur between oxyleghemoglobin and ferryl myoglobin or between ferryl l...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Mathieu C,Swaraj K,Davies MJ,Trinchant JC,Puppo A

    更新日期:1997-08-01 00:00:00

  • Nitric oxide (•NO) generation but not ROS plays a major role in silibinin-induced autophagic and apoptotic death in human epidermoid carcinoma A431 cells.

    abstract::Silibinin, a major active constituent of silymarin, is clinically used as a hepatoprotectant, and in recent years, it has been used for the treatment of cancer in China. Because the mechanism of silibinin action on cancer cells was still unclear, we investigated the contribution of silibinin to the induction of apopto...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Yu Y,Fan SM,Yuan SJ,Tashiro S,Onodera S,Ikejima T

    更新日期:2012-11-01 00:00:00

  • Effects of hydrogen peroxide on relaxation through the NO/sGC/cGMP pathway in isolated rat iliac arteries.

    abstract::The production of reactive oxygen species, including hydrogen peroxide (H(2)O(2)), is increased in diseased blood vessels. Although H(2)O(2) leads to impairment of the nitric oxide (NO)/soluble guanylate cyclase (sGC)/cGMP signaling pathway, it is not clear whether this reactive molecule affects the redox state of sGC...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Tawa M,Shimosato T,Iwasaki H,Imamura T,Okamura T

    更新日期:2015-01-01 00:00:00

  • Peroxyl radical scavenging activities of hamamelitannin in chemical and biological systems.

    abstract::The antioxidative activities of hamamelitannin (2',5-di-O-galloyl-hamamelose), gallic acid and dl-alpha-tocopherol against lipid peroxyl radicals were evaluated in chemical and biological systems. The peroxyl radical scavenging activity was evaluated by electron spin resonance (ESR) method in which both spin-trapping ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Masaki H,Atsumi T,Sakurai H

    更新日期:1995-05-01 00:00:00

  • Hemin-induced membrane sulfhydryl oxidation: possible involvement of thiyl radicals.

    abstract::Sublytic levels (microM) of hemin destabilized RBC membrane as indicated by ghost fragmentation pattern using a laser viscodiffractometer. Furthermore, electron microscopic study shows that 5 microM of hemin induced echinocytic transformation whereas higher hemin concentration (40 microM) induced spherocytic transform...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Chiu DT,Huang TY,Hung IJ,Wei JS,Liu TZ,Stern A

    更新日期:1997-07-01 00:00:00

  • Increase in insulin-induced relaxation of consecutive arterial segments toward the periphery: Role of vascular oxidative state.

    abstract:RATIONALE:The oxidative state has been implicated in the signaling of various vasomotor functions, yet its role regarding the vasomotor action of insulin is less known. OBJECTIVE:To investigate the insulin-evoked relaxations of consecutive arterial segments of different oxidative state and the role of extracellular si...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Szijártó IA,Molnár GA,Mikolás E,Fisi V,Laczy B,Gollasch M,Koller A,Wittmann I

    更新日期:2014-07-01 00:00:00

  • No significant paraquat-induced oxidative DNA damage in rats.

    abstract::The metabolism of paraquat generates oxygen radicals. Paraquat has thus been suggested as a model compound to induce oxidative damage to DNA, lipids and proteins in different cells and tissues, although experimental data are inconsistent. In order to explore the possibilities for an animal model of oxidative DNA damag...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Sørensen M,Loft S

    更新日期:2000-05-01 00:00:00

  • Carvedilol inhibition of lipid peroxidation. A new antioxidative mechanism.

    abstract::To define the molecular mechanism(s) of carvedilol inhibition of lipid peroxidation we have utilized model systems that allow us to study the different reactions involved in this complex process. Carvedilol inhibits the peroxidation of sonicated phosphatidylcholine liposomes triggered by FeCl2 addition whereas atenolo...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Tadolini B,Franconi F

    更新日期:1998-11-01 00:00:00

  • A novel method using 8-hydroperoxy-2'-deoxyguanosine formation for evaluating antioxidative potency.

    abstract::Degenerative diseases such as cancer are induced by oxidative genetic damage. Antioxidants can scavenge reactive oxygen species, but to prevent disease, they must do so quickly, before the DNA bases are damaged. In the present study, a novel method was established for evaluating the potency of antioxidants employing 2...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Sakakibara H,Ashida H,Kanazawa K

    更新日期:2002-03-01 00:00:00