Effects of esculetin on lipopolysaccharide (LPS)-induced acute lung injury via regulation of RhoA/Rho Kinase/NF-кB pathways in vivo and in vitro.


:The purpose of the present study was to investigate the protective effect of esculetin (ES) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the lung epithelial A549 cells. Mice were intragastrically administered with ES (20 and 40 mg/kg) 1 h prior to LPS challenge. ES pretreatment at doses of 20 and 40 mg/kg effectively attenuated LPS-induced lung histopathological change, myeloperoxidase or MPO activity, inflammatory cells infiltration, pulmonary wet-to-dry weight ratio, and the generation of pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in vivo and in vitro. Furthermore, we demonstrated that ES blocked the activation of NF-кB and RhoA/Rho kinase pathways in LPS-induced mice and A549 cells. The results suggested that ES exhibited protective effect on ALI and might attribute partly to the inhibition of NF-кB and RhoA/Rho kinase pathways in vivo and in vitro.


Free Radic Res


Free radical research


Chen T,Guo Q,Wang H,Zhang H,Wang C,Zhang P,Meng S,Li Y,Ji H,Yan T




Has Abstract


2015-01-01 00:00:00












  • Formation of guanidinosuccinic acid, a stable nitric oxide mimic, from argininosuccinic acid and nitric oxide-derived free radicals.

    abstract::Guanidinosuccinic acid (GSA) is noted for its nitric oxide (NO) mimicking actions such as vasodilatation and activation of the N-methyl-D-aspartate (NMDA) receptor. We have reported that GSA is the product of argininosuccinate (ASA) and some reactive oxygen species, mainly the hydroxyl radical. We tested for GSA synth...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Aoyagi K,Akiyama K,Shahrzad S,Tomida C,Hirayama A,Nagase S,Takemura K,Koyama A,Ohba S,Narita M

    更新日期:1999-07-01 00:00:00

  • Oxidative stress, d-ROMs test, and ceruloplasmin.

    abstract::Human serum samples were evaluated for oxidative stress with the d-ROMs test. The ceruloplasmin (CP) and copper contents of the samples was independently measured and compared to those calculated on the basis of the d-ROMs test results for pure CP solutions. The d-ROMs readings did not show any significant correlation...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Colombini F,Carratelli M,Alberti A

    更新日期:2016-01-01 00:00:00

  • Arctigenin suppresses transforming growth factor-β1-induced expression of monocyte chemoattractant protein-1 and the subsequent epithelial-mesenchymal transition through reactive oxygen species-dependent ERK/NF-κB signaling pathway in renal tubular epithe

    abstract::Transforming growth factor-β1 (TGF-β1) induces expression of the proinflammatory and profibrotic cytokine monocyte chemoattractant protein-1 (MCP-1) in tubular epithelial cells (TECs) and thereby contributes to the tubular epithelial-mesenchymal transition (EMT), which in turn leads to the progression of tubulointerst...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Li A,Wang J,Zhu D,Zhang X,Pan R,Wang R

    更新日期:2015-01-01 00:00:00

  • Pharmacological modulation of reactive oxygen species (ROS) improves the airway hyperresponsiveness by shifting the Th1 response in allergic inflammation induced by ovalbumin.

    abstract::Asthma is an allergic inflammation driven by the Th2 immune response with release of cytokines such as IL-4 and IL-13, which contribute to the airflow limitations and airway hyperresponsiveness (AHR). The involvement of oxidative stress in this process is well-established, but the specific role of the superoxide anion...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Nesi RT,Barroso MV,Souza Muniz V,de Arantes AC,Martins MA,Brito Gitirana L,Neves JS,Benjamim CF,Lanzetti M,Valenca SS

    更新日期:2017-07-01 00:00:00

  • Redox properties of the nitronyl nitroxide antioxidants studied via their reactions with nitroxyl and ferrocyanide.

    abstract::Nitronyl nitroxides (NNs) are the paramagnetic probes that are capable of scavenging physiologically relevant reactive oxygen (ROS) and nitrogen (RNS) species, namely superoxide, nitric oxide (NO), and nitroxyl (HNO). NNs are increasingly considered as potent antioxidants and potential therapeutic agents. Understandin...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Bobko AA,Khramtsov VV

    更新日期:2015-01-01 00:00:00

  • The role of tetrahydrobiopterin in superoxide generation from eNOS: enzymology and physiological implications.

    abstract::Tetrahydrobiopterin (BH4) is a ubiquitous pteridine metabolite that serves as a NOS cofactor. Recently, we showed that BH4 efficiently inhibits superoxide generation from the heme group at the oxygenase domain of eNOS. This role indicates that BH4 acts as a redox switch in the catalytic mechanism of the enzyme, which ...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Vásquez-Vivar J,Kalyanaraman B,Martásek P

    更新日期:2003-02-01 00:00:00

  • Severe total hepatic ischemia and reperfusion: relationship between very high alpha-tocopherol uptake and lipid peroxidation.

    abstract::Reperfusion injury of the liver occurs in liver transplantation and in major hepatectomies. It triggers a severe oxidative stress that leads to increased lipid peroxidation. In our study we examined the effect of parenteral supranutritional administration of alpha-tocopherol, a vitamin that plays a key role in the end...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Giakoustidis D,Kontos N,Iliadis S,Papageorgiou G,Tsantilas D,Spyridis C,Papazoglou K,Botsoglou N,Dimitriadou A,Giakoustidis E

    更新日期:2001-08-01 00:00:00

  • Hydrogen peroxide constricts rat arteries by activating Na+-permeable and Ca2+-permeable cation channels.

    abstract::Oxidative stress is associated with many cardiovascular diseases, such as hypertension and arteriosclerosis. Oxidative stress reportedly activates the L-type voltage-gated calcium channel (VDCCL) and elevates [Ca2+]i in many cells. However, how oxidative stress activates VDCCL under clinical setting and the consequenc...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Park HJ,Shin KC,Yoou SK,Kang M,Kim JG,Sung DJ,Yu W,Lee Y,Kim SH,Bae YM,Park SW

    更新日期:2019-01-01 00:00:00

  • xCT deficiency aggravates acetaminophen-induced hepatotoxicity under inhibition of the transsulfuration pathway.

    abstract::Cystine, an oxidized form of cysteine (Cys), is imported into cells via the protein xCT, which is also associated with the export of glutamate as the counter amino acid. In the current study, we attempted to rationalize roles of xCT in the livers of male mice. While xCT was not expressed in the livers of ordinary mice...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Kang ES,Lee J,Homma T,Kurahashi T,Kobayashi S,Nabeshima A,Yamada S,Seo HG,Miyata S,Sato H,Fujii J

    更新日期:2017-01-01 00:00:00

  • Hydroxyl radical scavenging activity of nonsteroidal anti-inflammatory drugs.

    abstract::The hydroxyl radical (.OH)-scavenging activity of d-2-[4-(3-methyl-2-thienyl)phenyl]propionic acid (M-5011), a novel nonsteroidal anti-inflammatory drug (NSAID), and that of several other NSAIDs were investigated by the hyaluronic acid (HA) degradation method and the electron spin resonance (ESR) spin-trapping techniq...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Kataoka M,Tonooka K,Ando T,Imai K,Aimoto T

    更新日期:1997-10-01 00:00:00

  • Regulation of gamma-glutamylcysteine synthetase subunit gene expression: insights into transcriptional control of antioxidant defenses.

    abstract::Gamma-glutamylcysteine synthetase (GCS; also referred to as glutamate-cysteine ligase, GLCL) catalyzes the rate-limiting reaction in glutathione (GSH) biosynthesis. The GCS holoenzyme is composed of a catalytic and regulatory subunit, each encoded by a unique gene. In addition to some conditions which specifically upr...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Wild AC,Mulcahy RT

    更新日期:2000-04-01 00:00:00

  • Carvedilol inhibition of lipid peroxidation. A new antioxidative mechanism.

    abstract::To define the molecular mechanism(s) of carvedilol inhibition of lipid peroxidation we have utilized model systems that allow us to study the different reactions involved in this complex process. Carvedilol inhibits the peroxidation of sonicated phosphatidylcholine liposomes triggered by FeCl2 addition whereas atenolo...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Tadolini B,Franconi F

    更新日期:1998-11-01 00:00:00

  • Anti-infective control in human bronchiolar epithelial cells by mucin phenotypic changes following uptake of N-acetyl-L-cysteine.

    abstract:PURPOSE:Aspiration pneumonia is infection of the respiratory tract resulting from accumulation of sputum in the larynx. N-acetyl-L-cysteine (NAC) might regulate mucin (MUC) expression and activate inherent anti-infective system in bronchiolar epithelial cells after cellular uptake, and therefore, serve as the preventat...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Koizumi C,Yamada M,Ishizaki K,Ueda T,Sakurai K

    更新日期:2015-01-01 00:00:00

  • The impact of different antioxidant agents alone or in combination on reactive oxygen species, antioxidant enzymes and cytokines in a series of advanced cancer patients at different sites: correlation with disease progression.

    abstract::In the present study we tested the ability of different antioxidant agents, used alone or in combination, to reduce the reactive oxygen species (ROS) levels and to increase the glutathione peroxidase (GPx) activity. Moreover, we tested the ability of such antioxidant agents to reduce the serum levels of proinflammator...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Mantovani G,Macciò A,Madeddu C,Mura L,Gramignano G,Lusso MR,Murgia V,Camboni P,Ferreli L,Mocci M,Massa E

    更新日期:2003-02-01 00:00:00

  • Diacylglycerol kinase alpha regulates globular adiponectin-induced reactive oxygen species.

    abstract::It has previously been reported that the globular form of adiponectin (gAd), mature adipocyte-derived cytokine, induced generation of reactive oxygen species (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. This study investigated whether diacylglycerol kinases (DGKs), enzymes functioning in sub...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Kamio N,Akifusa S,Yamashita Y

    更新日期:2011-03-01 00:00:00

  • Mechanism of metal-mediated DNA damage and apoptosis induced by 6-hydroxydopamine in neuroblastoma SH-SY5Y cells.

    abstract::6-Hydroxydopamine (6-OHDA) is a neurotoxin to produce an animal model of Parkinson's disease. 6-OHDA increased the formation of 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodG), a biomarker of oxidatively damaged DNA, and induced apoptosis in human neuroblastoma SH-SY5Y cells. Iron or copper chelators inhibited 6-OHDA-i...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Kobayashi H,Oikawa S,Umemura S,Hirosawa I,Kawanishi S

    更新日期:2008-07-01 00:00:00

  • A free radical initiator, 2,2'-azobis (2-aminopropane) dihydrochloride enhances hyperthermia-induced apoptosis in human uterine cervical cancer cell lines.

    abstract::Hyperthermia-induced apoptosis and its enhancement in the presence of a temperature-dependent free radical initiator, 2,2'-azobis (2-aminopropane) dihydrochloride (AAPH) were examined in human uterine cervical cancer cell lines, CaSki and HeLa. When both cell lines were treated with hyperthermia at 44 degrees C for 60...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Yuki H,Kondo T,Zhao QL,Fujiwara Y,Tanabe K,Ogawa R,Nakashima A,Fushiki H,Fujimura M,Saito S

    更新日期:2003-06-01 00:00:00

  • BSC2 enhances cell resistance to AmB by inhibiting oxidative damage in Saccharomyces cerevisiae.

    abstract::Amphotericin B has been the gold standard for the treatment of invasive mycosis for many years. Its resistance mechanisms are reported to be mainly related to the decrease of ergosterol content or the changes of cell wall. Previous study has shown that Saccharomyces cerevisiae strain lack of BSC2 was sensitive signifi...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Kong Y,Wang Q,Cao F,Zhang X,Fang Z,Shi P,Wang H,Shen Y,Huang Z

    更新日期:2020-04-01 00:00:00

  • Hemin-induced membrane sulfhydryl oxidation: possible involvement of thiyl radicals.

    abstract::Sublytic levels (microM) of hemin destabilized RBC membrane as indicated by ghost fragmentation pattern using a laser viscodiffractometer. Furthermore, electron microscopic study shows that 5 microM of hemin induced echinocytic transformation whereas higher hemin concentration (40 microM) induced spherocytic transform...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Chiu DT,Huang TY,Hung IJ,Wei JS,Liu TZ,Stern A

    更新日期:1997-07-01 00:00:00

  • Effect of antioxidants on ammonia induced CNS-renal pathobiology in sheep.

    abstract::The effect of antioxidants on ammonia induced CNS and renal pathobiology in 10 sheep infested by L. Cuprina larvae was investigated. The condition produces severe dermatitis, proliferation of macrophages and hyperammonaemia, and free radicals may therefore be involved in the pathogenesis. Five of the sheep (treated gr...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Guerrini VH

    更新日期:1994-07-01 00:00:00

  • DNA strand scission by the nephrotoxin [2,2'-bipyridine]-3,3',4,4'-tetrol-1,1'-dioxide and related compounds in the presence of iron.

    abstract::The capacity of non-illuminated nephrotoxin orellanine ([2,2'-bipyridine]-3,3',4,4'-tetrol-1,1'-dioxide) to induce DNA damage in the presence of ferrous iron and dioxygen has been evaluated. Maximal single-strand breaks in plasmid DNA were obtained with a metal to ligand ratio 1:3. Instantaneous oxidation of Fe2+ in p...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Cantin-Esnault D,Oubrahim H,Richard JM

    更新日期:2000-08-01 00:00:00

  • Suppression of mitochondrial NADP(+)-dependent isocitrate dehydrogenase activity enhances curcumin-induced apoptosis in HCT116 cells.

    abstract::Curcumin is a polyphenol derived from the plant Curcuma longa that induces apoptotic cell death in malignant cancer cell lines. It has been shown previously that mitochondrial NADP(+)-dependent isocitrate dehydrogenase (IDPm) plays an essential role in defense against oxidative stress by supplying NADPH for antioxidan...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Jung KH,Park JW

    更新日期:2011-04-01 00:00:00

  • GSK-3β inhibition by curcumin mitigates amyloidogenesis via TFEB activation and anti-oxidative activity in human neuroblastoma cells.

    abstract::The translocation of transcription factor EB (TFEB) to the nucleus plays a pivotal role in the regulation of basic cellular processes, such as lysosome biogenesis and autophagy. Autophagy is an intracellular degradation system that delivers cytoplasmic constituents to the lysosome, which is important in maintaining ce...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Song HC,Chen Y,Chen Y,Park J,Zheng M,Surh YJ,Kim UH,Park JW,Yu R,Chung HT,Joe Y

    更新日期:2020-07-28 00:00:00

  • Reduced glomerular filtration rate, inflammation and HDL cholesterol as main determinants of superoxide production in non-dialysis chronic kidney disease patients.

    abstract::Enhanced oxidative stress partly resulting from an over-production of superoxide anion (O(2)(•-)) represents a novel and particular risk factor in chronic kidney disease (CKD) patients. This study was therefore designed to evaluate O(2)(•-) determinants in this population. O(2)(•-) production was evaluated using chemi...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Morena M,Patrier L,Jaussent I,Bargnoux AS,Dupuy AM,Badiou S,Leray-Moragues H,Klouche K,Canaud B,Cristol JP

    更新日期:2011-06-01 00:00:00

  • Effect of complexation with randomly methylated beta-cyclodextrin on the aqueous solubility, photostability and antioxidant activity of an indolinonic nitroxide radical.

    abstract::The interaction between the hydrophobic indolinonic nitroxide radical, 1,2-dihydro-2-methyl-2-phenyl-3H-indole-3-one-1-oxyl and hydrophilic alpha-, beta- and gamma-cyclodextrin derivatives was investigated in water by phase-solubility analysis. Among the studied cyclodextrins, random methyl-beta-cyclodextrin (RM-beta-...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Damiani E,Tursilli R,Casolari A,Astolfi P,Greci L,Scalia S

    更新日期:2005-01-01 00:00:00

  • Toxicity of polyunsaturated fatty acid esters for human monocyte-macrophages: the anomalous behaviour of cholesteryl linolenate.

    abstract::We have investigated the toxicity to human monocytemacrophages, and susceptibility to oxidation, of different individual dietary fatty acids in cholesterol esters and triglycerides, added to the cell cultures as coacervates with bovine serum albumin. Toxicity was assessed using release of radioactivity from cells prel...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Hardwick SJ,Carpenter KL,Law NS,Van Der Veen C,Marchant CE,Hird R,Mitchinson MJ

    更新日期:1997-04-01 00:00:00

  • Induction of glutathione peroxidase in response to inactivation by nitric oxide.

    abstract::To determine effect of nitric oxide (NO) on cellular glutathione peroxidase (GPX) level in living cells, we measured the activity, protein and mRNA of GPX in rat kidney (KNRK) cells under a high NO condition. Combined treatment of lipopolysaccharide (LPS, 1 microgram/ml) and tumor necrosis factor-alpha (TNF-alpha, 50 ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Dobashi K,Asayama K,Nakane T,Kodera K,Hayashibe H,Nakazawa S

    更新日期:2001-09-01 00:00:00

  • The influence of oxidative bursts of phagocytes on red blood cell oxidation in anemic cattle infected with Theileria sergenti.

    abstract::The primary clinical symptom of Japanese bovine theileriosis, caused by the intraerythrocytic protozoan Theileria sergenti, is anemia, but the underlying mechanism of this anemia remains unknown. To elucidate the pathogenesis of anemia developing in bovine theileriosis, we investigated the relationship between oxidati...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Shiono H,Yagi Y,Chikayama Y,Miyazaki S,Nakamura I

    更新日期:2003-11-01 00:00:00

  • Role of thioredoxin peroxidase in aging of stationary cultures of Saccharomyces cerevisiae.

    abstract::A soluble protein from Saccharomyces cerevisiae acts as a peroxidase but requires a NADPH-dependent thioredoxin system and was named thioredoxin peroxidase (TPx). The role of TPx in aging of stationary cultures of S. cerevisiae was investigated in a wild-type strain and a mutant yeast strain in which the tsa gene that...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Lee JH,Park JW

    更新日期:2004-03-01 00:00:00

  • Glucose accelerates copper- and ceruloplasmin-induced oxidation of low-density lipoprotein and whole serum.

    abstract::Glucose at pathophysiological concentrations was able to accelerate copper-induced oxidation of isolated low-density lipoprotein (LDL) and whole serum. The efficiency of glucose was favored under the following circumstances: (a) when LDL oxidation was induced by low copper concentration, (b) when LDL was partly oxidiz...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Leoni V,Albertini R,Passi A,Abuja PM,Borroni P,D'Eril GM,De Luca G

    更新日期:2002-05-01 00:00:00