Effects of esculetin on lipopolysaccharide (LPS)-induced acute lung injury via regulation of RhoA/Rho Kinase/NF-кB pathways in vivo and in vitro.


:The purpose of the present study was to investigate the protective effect of esculetin (ES) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the lung epithelial A549 cells. Mice were intragastrically administered with ES (20 and 40 mg/kg) 1 h prior to LPS challenge. ES pretreatment at doses of 20 and 40 mg/kg effectively attenuated LPS-induced lung histopathological change, myeloperoxidase or MPO activity, inflammatory cells infiltration, pulmonary wet-to-dry weight ratio, and the generation of pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in vivo and in vitro. Furthermore, we demonstrated that ES blocked the activation of NF-кB and RhoA/Rho kinase pathways in LPS-induced mice and A549 cells. The results suggested that ES exhibited protective effect on ALI and might attribute partly to the inhibition of NF-кB and RhoA/Rho kinase pathways in vivo and in vitro.


Free Radic Res


Free radical research


Chen T,Guo Q,Wang H,Zhang H,Wang C,Zhang P,Meng S,Li Y,Ji H,Yan T




Has Abstract


2015-01-01 00:00:00












  • Pharmacokinetics and metabolism of dietary flavonoids in humans.

    abstract::Flavonoids are components of fruit and vegetables that may be beneficial in the prevention of disease such as cancer and cardiovascular diseases. Their beneficial effects will be dependent upon their uptake and disposition in tissues and cells. The metabolism and pharmacokinetics of flavonoids has been an area of acti...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Manach C,Donovan JL

    更新日期:2004-08-01 00:00:00

  • Detection of a stable free radical in the B2 subunit of the manganese ribonucleotide reductase (Mn-RRase) of Corynebacterium ammoniagenes.

    abstract::Ribonucleotide reductases catalyze the irreversible reductive formation of 2'-deoxyribonucleotides required for DNA replication and cell proliferation, and a radical mechanism was assumed to be involved in this reaction. In order to search for a radical in the aerobic manganese ribonucleotide reductase (Mn-RRase) by e...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Griepenburg U,Lassmann G,Auling G

    更新日期:1996-06-01 00:00:00

  • Characterization of hydrogen peroxide and superoxide degrading pathways of Aspergillus niger catalase: a steady-state analysis.

    abstract::The oxidized intermediates generated upon exposure of Aspergillus niger catalase to hydrogen peroxide and superoxide radical fluxes were examined with UV-visible spectrophotometry. Hydrogen peroxide and superoxide radical were generated by means of glucose/glucose oxidase and xanthine/xanthine oxidase systems. Serial ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Lardinois OM,Rouxhet PG

    更新日期:1994-01-01 00:00:00

  • Inhibition of cell growth by overexpression of manganese superoxide dismutase (MnSOD) in human pancreatic carcinoma.

    abstract::Manganese superoxide dismutase (MnSOD) levels have been found to be low in human pancreatic cancer [Pancreas 26, (2003), 23] and human pancreatic cancer cell lines [Cancer Res. 63, (2003), 1297] when compared to normal human pancreas. We hypothesized that stable overexpression of pancreatic cancer cells with MnSOD cDN...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Ough M,Lewis A,Zhang Y,Hinkhouse MM,Ritchie JM,Oberley LW,Cullen JJ

    更新日期:2004-11-01 00:00:00

  • Diacylglycerol kinase alpha regulates globular adiponectin-induced reactive oxygen species.

    abstract::It has previously been reported that the globular form of adiponectin (gAd), mature adipocyte-derived cytokine, induced generation of reactive oxygen species (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. This study investigated whether diacylglycerol kinases (DGKs), enzymes functioning in sub...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Kamio N,Akifusa S,Yamashita Y

    更新日期:2011-03-01 00:00:00

  • The role of endothelial dysfunction and oxidative stress in cerebrovascular diseases.

    abstract::Cerebrovascular diseases (CBD) are one of the most dangerous complications of atherosclerosis. The clinical consequences of CBD deeply impact quality of life and the prognosis of patients. Atherosclerosis is the main cause of CBD development. Hypertension, dyslipidemia, diabetes, smoking, obesity, and other risk facto...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Scicchitano P,Cortese F,Gesualdo M,De Palo M,Massari F,Giordano P,Ciccone MM

    更新日期:2019-06-01 00:00:00

  • Vitamin E-coated filter decreases levels of free 4-hydroxyl-2-nonenal during haemodialysis sessions.

    abstract::Uraemic subjects undergoing chronic haemodialysis show increased oxidative stress. The use of non-biocompatible filters and reduced antioxidative defences are important sources of reactive oxygen species (ROS) release. The highly oxidative environment accelerates the onset and progression of tissue damage and atherosc...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Odetti P,Traverso N,Monacelli F,Menini S,Vazzana J,Tasso B,Pronzato MA,Robaudo C,Deferrari G

    更新日期:2006-02-01 00:00:00

  • pLG72 induces superoxide radicals via interaction and aggregation with SOD1.

    abstract::G72 has been characterised as a susceptibility gene that can have wide-ranging effects in a number of neurodegenerative diseases, including schizophrenia and major depression. Indeed, its product, pLG72, is a potential serum biomarker for schizophrenia. Previous transcriptomic and biochemical studies have indicated th...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Wang M,Saw HP,Cui FF,Lin SY,Chang HT,Chiu CD

    更新日期:2018-09-01 00:00:00

  • Hydrogen-rich water improves cognitive impairment gender-dependently in APP/PS1 mice without affecting Aβ clearance.

    abstract::Alzheimer's disease (AD) is characterised as a provoked inflammatory response and oxidative stress along with amyloid β peptide (Aβ) deposition and neurofibrillary tangles in the brain, and effective treatment is greatly needed. Molecular hydrogen, which has been proposed to be an antioxidant that selectively reduces ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Hou C,Peng Y,Qin C,Fan F,Liu J,Long J

    更新日期:2018-12-01 00:00:00

  • Iron prevents ascorbic acid (vitamin C) induced hydrogen peroxide accumulation in copper contaminated drinking water.

    abstract::Ascorbic acid (vitamin C) induced hydrogen peroxide (H(2)O(2)) formation was measured in household drinking water and metal supplemented Milli-Q water by using the FOX assay. Here we show that ascorbic acid readily induces H(2)O(2) formation in Cu(II) supplemented Milli-Q water and poorly buffered household drinking w...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Jansson PJ,Lindqvist C,Nordström T

    更新日期:2005-11-01 00:00:00

  • Metallothionein over-expression in podocytes reduces adriamycin nephrotoxicity.

    abstract::Adriamycin (ADR) is nephrotoxic. One component of ADR-induced nephropathy may be oxidative stress. This study used a recently developed line of transgenic mice (Nmt) on the FVB background strain, which over-express the antioxidant protein metallothionein (MT) in podocytes. Cultured podocytes from Nmt mice were resista...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Yang L,Zheng S,Epstein PN

    更新日期:2009-02-01 00:00:00

  • Mechanism of metal-mediated DNA damage and apoptosis induced by 6-hydroxydopamine in neuroblastoma SH-SY5Y cells.

    abstract::6-Hydroxydopamine (6-OHDA) is a neurotoxin to produce an animal model of Parkinson's disease. 6-OHDA increased the formation of 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodG), a biomarker of oxidatively damaged DNA, and induced apoptosis in human neuroblastoma SH-SY5Y cells. Iron or copper chelators inhibited 6-OHDA-i...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Kobayashi H,Oikawa S,Umemura S,Hirosawa I,Kawanishi S

    更新日期:2008-07-01 00:00:00

  • Alteration of antioxidants during the progression of heart disease in streptozotocin-induced diabetic rats.

    abstract::Involvement of oxidative stress is implicated in the progression of complication of diabetes mellitus. With respect to heart diseases, we have studied role of oxidative stress/antioxidants using rats treated with streptozotocin to induce diabetes (DM). Hemodynamic and echocardiographic measurements showed thickening o...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Doi K,Sawada F,Toda G,Yamachika S,Seto S,Urata Y,Ihara Y,Sakata N,Taniguchi N,Kondo T,Yano K

    更新日期:2001-03-01 00:00:00

  • The heme oxygenase pathway and its interaction with nitric oxide in the control of cellular homeostasis.

    abstract::Heme oxygenase is the rate limiting enzyme in heme degradation to carbon monoxide (CO), iron and bilirubin. The inducible isoform of the protein, heme oxygenase-1 (HO-1), is susceptible to up-regulation by a diverse variety of conditions and agents in mammalian tissue, leading to the common conception that HO-1 is a s...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Foresti R,Motterlini R

    更新日期:1999-12-01 00:00:00

  • Lymphocyte antioxidant response and H2O2 production after a swimming session: gender differences.

    abstract::This study evaluated the gender differences in response to intense exercise on lymphocyte hydrogen peroxide production, nitric oxide handling and mitochondrial superoxide dismutase (MnSOD) activity and gene expression. Fifteen males and nine females participated voluntarily in the study and performed a swimming sessio...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Sureda A,Ferrer MD,Tauler P,Tur JA,Pons A

    更新日期:2008-04-01 00:00:00

  • xCT deficiency aggravates acetaminophen-induced hepatotoxicity under inhibition of the transsulfuration pathway.

    abstract::Cystine, an oxidized form of cysteine (Cys), is imported into cells via the protein xCT, which is also associated with the export of glutamate as the counter amino acid. In the current study, we attempted to rationalize roles of xCT in the livers of male mice. While xCT was not expressed in the livers of ordinary mice...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Kang ES,Lee J,Homma T,Kurahashi T,Kobayashi S,Nabeshima A,Yamada S,Seo HG,Miyata S,Sato H,Fujii J

    更新日期:2017-01-01 00:00:00

  • Singlet oxygen quenching and radical scavenging capacities of structurally-related flavonoids present in Zuccagnia punctata Cav.

    abstract::The singlet oxygen (1O2) quenching and free radical (DPPH(*), ABTS(* +) and O2(* -)) scavenging ability of three structurally-related flavonoids (7-hydroxyflavanone HF, 2',4'-dihydroxychalcone DHC and 3,7-dihydroxyflavone DHF) present in the Argentinean native shrub Zuccagnia punctata Cav. were studied in solution by ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Vieyra FE,Boggetti HJ,Zampini IC,Ordoñez RM,Isla MI,Alvarez RM,De Rosso V,Mercadante AZ,Borsarelli CD

    更新日期:2009-06-01 00:00:00

  • Resveratrol treatment protects against doxorubicin-induced cardiotoxicity by alleviating oxidative damage.

    abstract::The possible protective effects of resveratrol (RVT) against cardiotoxicity were investigated in Wistar albino rats treated with saline, saline+doxorubicin (DOX; 20 mg/kg) or RVT (10 mg/kg)+DOX. Blood pressure and heart rate were recorded on the 1st week and on the 7th week, while cardiomyopathy was assessed using tra...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Tatlidede E,Sehirli O,Velioğlu-Oğünc A,Cetinel S,Yeğen BC,Yarat A,Süleymanoğlu S,Sener G

    更新日期:2009-03-01 00:00:00

  • Coenzyme Q10 serves to couple mitochondrial oxidative phosphorylation and fatty acid β-oxidation, and attenuates NLRP3 inflammasome activation.

    abstract::Multiple acyl-CoA dehydrogenase deficiency (MADD), an autosomal recessive metabolic disorder of fatty acid metabolism, is mostly caused by mutations in the ETFA, ETFB or ETFDH genes that result in dysfunctions in electron transfer flavoprotein (ETF) or electron transfer flavoprotein-ubiquinone dehydrogenase (ETFDH). I...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Chokchaiwong S,Kuo YT,Lin SH,Hsu YC,Hsu SP,Liu YT,Chou AJ,Kao SH

    更新日期:2018-12-01 00:00:00

  • Peptides as antioxidants and carbonyl quenchers in biological model systems.

    abstract::Subjecting selected peptides to in vitro analyses covering their ability to interfere with the lipid oxidation chain reaction as well as to protect proteins from direct and indirect oxidation has provided the basis for a more detailed understanding of peptide-mediated protection in biological systems. The efficiency o...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Jongberg S,Carlsen CU,Skibsted LH

    更新日期:2009-10-01 00:00:00

  • Inhibitory effects of post low dose gamma-ray irradiation on ferric-nitrilotriacetate-induced mice liver damage.

    abstract::We studied the effects of a single post whole-body low-dose irradiation (50 cGy of gamma-ray) on mice with ferric nitrilotriacetate (Fe3+-NTA)-induced transient hepatopathy. As a result, low-dose irradiation accelerated the rate of recovery. Based on the changes in glutamic oxaloacetic transaminase (GOT) activities, g...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Yamaoka K,Kojima S,Nomura T

    更新日期:2000-03-01 00:00:00

  • Liver microsomal parameters related to oxidative stress and antioxidant systems in hyperthyroid rats subjected to acute lindane treatment.

    abstract::Liver microsomal functions related to xenobiotic biotransformation and free radical production were studied in control rats and in animals subjected to L-3,3',5-triiodothyronine (T3) and/or lindane administration as possible mechanisms contributing to oxidative stress, in relation to the activity of enzymes (superoxid...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Simon Giavarotti KA,Rodrigues L,Rodrigues T,Junqueira VB,Videla LA

    更新日期:1998-07-01 00:00:00

  • Nucleoside transporters are critical to the uptake and antioxidant activity of 7,8-dihydroneopterin in monocytic cells.

    abstract::7,8-Dihydroneopterin protects cells intracellularly from oxidative stress-induced death, but its mode of transport across the cell membrane is unknown. Nucleosides, such as guanosine, are transported via nucleoside transporters of the equilibrative and concentrative forms. Therefore, the objective of this study was to...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Janmale TV,Lindsay A,Gieseg SP

    更新日期:2020-05-01 00:00:00

  • Antioxidant properties of catechins and proanthocyanidins: effect of polymerisation, galloylation and glycosylation.

    abstract::A range of catechins and oligomeric procyanidins was purified by high performance liquid chromatography (HPLC) from grape seed, apple skin, lentil and almond flesh. Catechins, galloylated epicatechin, glycosylated catechin, procyanidin dimers, galloylated dimers, trimer, and tetramer species were all identified, purif...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Plumb GW,De Pascual-Teresa S,Santos-Buelga C,Cheynier V,Williamson G

    更新日期:1998-10-01 00:00:00

  • Effects of various vitamins and coenzymes Q on reactions involving alpha-hydroxyl-containing radicals.

    abstract::Effects of vitamins B, C, E, K and P, as well as coenzymes Q, on formation of final products of radiation-induced free-radical transformations of ethanol, ethylene glycol, alpha-methylglycoside and glucose in aqueous solutions were studied. Based on the obtained results, it can be concluded that there are substances a...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Shadyro OI,Sosnovskaya AA,Edimecheva IP,Grintsevich IB,Lagutin PY,Alekseev AV,Kazem K

    更新日期:2005-07-01 00:00:00

  • Ceruloplasmin decreases respiratory burst reaction during pregnancy.

    abstract::Testing of pregnant women reveals weakening of neutrophil-mediated effector functions, such as reactive oxygen species generation. This study provides data confirming the phenomenon, gained through application of the flow cytometry technique. Key factors influencing neutrophil functional activity in blood plasma of pr...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Varfolomeeva EY,Semenova EV,Sokolov AV,Aplin KD,Timofeeva KE,Vasilyev VB,Filatov MV

    更新日期:2016-08-01 00:00:00

  • Synthesis and spin trapping properties of polystyrene supported trifluoromethylated cyclic nitrones.

    abstract::Polystyrene supported fluorinated cyclic nitrone spin-traps: Resin-2-HFDMPO (2-hydroxymethyl-2-methyl-5-(trifluoromethyl)-3,4-dihydro-2H-pyrrole-1-oxide) and Resin-2-PFDMPO (2-(3-hydroxypropyl)-2-methyl-5-(trifluoromethyl)-3,4-dihydro-2H-pyrrole 1-oxide) containing a trifluoromethyl pyrroline-N-oxide core were develop...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Earla A,Walter ED,Braslau R

    更新日期:2019-12-01 00:00:00

  • Analysis of the oxidative stress response of Penicillium chrysogenum to menadione.

    abstract::The intracellular superoxide and glutathione disulphide concentrations increased in Penicillium chrysogeum treated with 50, 250 or 500 microM menadione (MQ). A significant increase in the intracellular peroxide concentration was also observed when mycelia were exposed to 250 or 500 microM MQ. The specific activity of ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Emri T,Pócsi I,Szentirmai A

    更新日期:1999-02-01 00:00:00

  • Kinetics of 3-nitrotyrosine modification on exposure to hypochlorous acid.

    abstract::The markers 3-nitrotyrosine and 3-chlorotyrosine are measured as surrogates for reactive nitrogen species and hypochlorous acid respectively, which are both elevated in inflamed human tissues. Previous studies reported a loss of 3-nitrotyrosine when exposed to hypochlorous acid, suggesting that observations of 3-nitro...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Curtis MP,Neidigh JW

    更新日期:2014-11-01 00:00:00

  • In vitro antioxidant capacity and antigenotoxic properties of protoporphyrin and structurally related tetrapyrroles.

    abstract::The antioxidant properties of protoporphyrin IX and related tetrapyrroles are poorly characterized. Therefore, eight tetrapyrroles, five of which are produced in vivo, were tested to assess their antioxidant capacities in the Salmonella reverse mutation, TEAC, FRAP and ORAC assays. Tertiary-butyl hydroperoxide (tert-B...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Mölzer C,Huber H,Steyrer A,Ziesel G,Ertl A,Plavotic A,Wallner M,Bulmer AC,Wagner KH

    更新日期:2012-11-01 00:00:00