Abstract:
:Nine male volunteers were given 36 g of cocoa powder (containing 2610 mg of polyphenols) per day with sugar and 6 volunteers received an equivalent amount of sugar for 2 weeks. Conjugated diene production in LDL induced by 2-2' azobis(4-methoxy-2,4-dimethylvaleronitrile) (V-70) and copper ion were evaluated. The lag time was significantly prolonged at 1 and 2 weeks in V-70 and at 2 weeks in copper ion after cocoa powder consumption. The level of excretion of epicatechin in urine was significantly higher in the cocoa group than that in the control group. In conclusion, the antioxidants in cocoa powder might be absorbed and increase the resistance of human LDL to oxidation.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Osakabe N,Baba S,Yasuda A,Iwamoto T,Kamiyama M,Takizawa T,Itakura H,Kondo Kdoi
10.1080/10715760100300091keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
93-9issue
1eissn
1071-5762issn
1029-2470journal_volume
34pub_type
杂志文章abstract::The effects of various free radicals derived from 6-formylpterin (6-FP), alpha-phenyl-tert-butyl nitrone (PBN) and 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) combined with hyperthermia, on gene expression in similarly enhanced apoptosis of human lymphoma U937 cells were investigated using cDNA microarrays c...
journal_title:Free radical research
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abstract::The intracellular superoxide and glutathione disulphide concentrations increased in Penicillium chrysogeum treated with 50, 250 or 500 microM menadione (MQ). A significant increase in the intracellular peroxide concentration was also observed when mycelia were exposed to 250 or 500 microM MQ. The specific activity of ...
journal_title:Free radical research
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abstract::The response of HeLa cells to subcellular spatially localized two-photon irradiation of a singlet oxygen photosensitizer (protoporphyrin IX, PpIX) using a focused laser was assessed. Upon irradiation under these conditions, a localized population of PpIX excited states can be produced with meaningful intracellular spa...
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journal_title:Free radical research
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abstract::Reactive oxygen species (ROS) are involved in the mechanism of photoaging and carcinogenesis. Skin is endowed with antioxidant enzymes including superoxide dismutases (SOD): cytosolic copper zinc SOD and mitochondrial manganese SOD. The aim of our study was to estimate the protective effect of manganese against oxidat...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769509065255
更新日期:1995-10-01 00:00:00
abstract::Oxygen radical generating systems, namely, Cu(II)/ H2O2, Cu(II)/ascorbate, Cu(II)/NAD(P)H, Cu(II)/ H2O2/catecholamine and Cu(II)/H2O2/SH-compounds irreversibly inhibited yeast glutathione reductase (GR) but Cu(II)/H2O2 enhanced the enzyme diaphorase activity. The time course of GR inactivation by Cu(II)/H2O2 dependent...
journal_title:Free radical research
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journal_title:Free radical research
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更新日期:1996-07-01 00:00:00
abstract::Many epidemiological data indicate the presence of a strong familial component of longevity that is largely determined by genetics, and a number of possible associations between longevity and allelic variants of genes have been described. A breakthrough strategy to get insight into the genetics of longevity is the stu...
journal_title:Free radical research
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更新日期:2006-12-01 00:00:00
abstract::Amphotericin B has been the gold standard for the treatment of invasive mycosis for many years. Its resistance mechanisms are reported to be mainly related to the decrease of ergosterol content or the changes of cell wall. Previous study has shown that Saccharomyces cerevisiae strain lack of BSC2 was sensitive signifi...
journal_title:Free radical research
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更新日期:2020-04-01 00:00:00
abstract::APE1/Ref-1, the mammalian ortholog of E. coli Xth, and a multifunctional protein possessing both DNA repair and transcriptional regulatory activities, has dual role in controlling cellular response to oxidative stress. It is rate-limiting in repair of oxidative DNA damage including strand breaks and also has co-transc...
journal_title:Free radical research
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更新日期:2008-01-01 00:00:00
abstract::Validation of markers requires two different steps. The first one is the analytical validation including analytical procedures, definition of standard reference, and round-tests analysis. The second one is the assessment of the biological relevance of the selected marker. This biological relevance may be in the field ...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:
更新日期:2000-11-01 00:00:00
abstract::Silibinin, a major active constituent of silymarin, is clinically used as a hepatoprotectant, and in recent years, it has been used for the treatment of cancer in China. Because the mechanism of silibinin action on cancer cells was still unclear, we investigated the contribution of silibinin to the induction of apopto...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2012.715369
更新日期:2012-11-01 00:00:00
abstract::Peroxiredoxin 1 (PRDX1) is an antioxidant enzyme that, when secreted, can act as a proinflammatory signal. Here we studied the regulation of intracellular PRDX1 by lipopolysaccharide (LPS) and interferon-gamma (IFN-γ) in the RAW 264.7 mouse macrophage cell line. While LPS or IFN-γ alone did not affect PRDX1 protein le...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2019.1637863
更新日期:2019-08-01 00:00:00
abstract::Myeloperoxidase (MPO), a major constituent of neutrophils, catalyzes the production of hypochlorous acid (HOCl) from hydrogen peroxide (H2O2) and chloride anion. We have previously reported that MPO-deficient (MPO(-/-)) neutrophils produce greater amount of macrophage inflammatory protein-2 (MIP-2) in vitro than do wi...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2013.778990
更新日期:2013-05-01 00:00:00
abstract::Gastric inflammation is a highly complex biochemical protective response to cellular/tissue injury. When this process occurs in an uncontrolled manner, the result is excessive cellular/tissue damage that results chronic inflammation and destruction of normal tissue. Current evidence suggests that Helicobacter pylori (...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760000301301
更新日期:2000-12-01 00:00:00
abstract::Previous studies have shown that glucose-6-phosphate dehydrogenase (G6PD)-deficient cells are under increased oxidative stress and undergo premature cellular senescence. The present study demonstrates that G6PD-deficient cells cultured under 3% oxygen concentration had an extended replicative lifespan, as compared wit...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760601184819
更新日期:2007-05-01 00:00:00
abstract::A range of catechins and oligomeric procyanidins was purified by high performance liquid chromatography (HPLC) from grape seed, apple skin, lentil and almond flesh. Catechins, galloylated epicatechin, glycosylated catechin, procyanidin dimers, galloylated dimers, trimer, and tetramer species were all identified, purif...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769800300391
更新日期:1998-10-01 00:00:00
abstract::We have investigated the toxicity to human monocytemacrophages, and susceptibility to oxidation, of different individual dietary fatty acids in cholesterol esters and triglycerides, added to the cell cultures as coacervates with bovine serum albumin. Toxicity was assessed using release of radioactivity from cells prel...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709097815
更新日期:1997-04-01 00:00:00
abstract::The hydroxyl radical adducts of 5,5 dimethyl-1-pyrolline-N-oxide (DMPO) and 3,3,5,5 tetramethyl-1-pyrolline-N-oxide (TMPO) formed in the presence of hydrogen peroxide and FeII are normally quite stable, but in the presence of 5-20 micromolar myoglobin their ESR signals decay rapidly. This decay probably reflects furth...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769409145632
更新日期:1994-05-01 00:00:00
abstract::Reperfusion injury of the liver occurs in liver transplantation and in major hepatectomies. It triggers a severe oxidative stress that leads to increased lipid peroxidation. In our study we examined the effect of parenteral supranutritional administration of alpha-tocopherol, a vitamin that plays a key role in the end...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100300641
更新日期:2001-08-01 00:00:00
abstract::Membrane rafts (MRs) are specialized microdomains in the cell membrane with an altered lipid composition. Upon various stimulations, MRs can be clustered to aggregate or recruit NADPH oxidase sub-units and related proteins to form MR redox signalosomes in the membrane of cells like vascular endothelial cells (ECs). Mu...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2010.485994
更新日期:2010-08-01 00:00:00
abstract::Oxidative stress appears to be implicated in the pathogenesis of various diseases including hepatotoxicity. Although intracellular Ca2+ signals have been suggested to play a role in the oxidative damage of hepatocytes, the sources and effects of oxidant-induced intracellular Ca2+ increases are currently debatable. Thu...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760000301431
更新日期:2000-09-01 00:00:00
abstract::The purpose of this double clinical study was (1) to evaluate the effect of one single intake (300 ml) of red wine (RW) on the plasma antioxidant capacity (pAOC) and plasma phenolics over the 24-h time period following the intake, and (2) to compare the long-term effects of daily intakes (250 ml/d) of RW, white wine (...
journal_title:Free radical research
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doi:10.1080/10715760310001598097
更新日期:2003-09-01 00:00:00
abstract::In this study, three rapid assay techniques for the determination of glutathione, one enzymatic, one fluorometric and one newly patented colorimetric method, were compared by measuring reduced (GSH) and oxidized (GSSG) glutathione in guinea-pig heart and liver. The HPLC technique was used as a standard, since it is co...
journal_title:Free radical research
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doi:10.3109/10715769709084481
更新日期:1997-05-01 00:00:00
abstract::The host inflammatory response appears to be an important contributor to the pathogenesis of human viral respiratory illness. Virus-induced oxidative stress appears to mediate an early phase of elaboration of the proinflammatory cytokine interleukin-8 by respiratory epithelial cells. The purpose of these studies was t...
journal_title:Free radical research
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更新日期:2002-10-01 00:00:00
abstract::The translocation of transcription factor EB (TFEB) to the nucleus plays a pivotal role in the regulation of basic cellular processes, such as lysosome biogenesis and autophagy. Autophagy is an intracellular degradation system that delivers cytoplasmic constituents to the lysosome, which is important in maintaining ce...
journal_title:Free radical research
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更新日期:2020-07-28 00:00:00
abstract::The use of salicylate as a chemical trap for .OH represents a simple and convenient alternative to the use of spin trapping techniques to study oxidative injury in isolated perfused organs. In these systems, salicylate is included in the perfusion buffer at concentrations ranging from 0.1 to 2 mM depending on the dete...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715769409056588
更新日期:1994-11-01 00:00:00
abstract::Neopterin and the reduced form, 7,8-dihydroneopterin (78NP) are pteridines released from macrophages when stimulated with gamma-interferon in vivo. The role of 78NP in inflammatory response is unknown though neopterin has been used clinically as a marker of immune cell activation, due to its very fluorescent nature. U...
journal_title:Free radical research
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doi:10.1080/10715760100300121
更新日期:2001-02-01 00:00:00
abstract::Radicals from one-electron oxidation of 4-hydroxycinnamate, ferulate and 3,4-dihydroxycinnamate have been formed by reaction with the oxidising triplet state of duroquinone. All three compounds react with triplet duroquinone with second order rate constants close to the diffusion-controlled limit. The identity of the ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100300621
更新日期:2001-07-01 00:00:00
abstract::The biomimetic model of micelles of linoleic acid containing 2-mercaptoethanol and the antioxidant was examined under gamma irradiation up to 400 Gy in aerobic or deoxygenated conditions where thiyl radicals are the main reactive species. Lipid peroxidation was retarded by ascorbic acid and α-tocopherol, whereas this ...
journal_title:Free radical research
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更新日期:2016-11-01 00:00:00