Abstract:
:The use of salicylate as a chemical trap for .OH represents a simple and convenient alternative to the use of spin trapping techniques to study oxidative injury in isolated perfused organs. In these systems, salicylate is included in the perfusion buffer at concentrations ranging from 0.1 to 2 mM depending on the detection apparatus employed. In our studies, we have used a coulometric detector, which has a theoretical efficiency of 100% as compared to 1-5% for the standard glassy carbon electrode. We have been able to generate reproducible results by inclusion of only 100 microM salicylate, a concentration demonstrated not to affect pre- or post-ischemic cardiac function. In initial studies, we observed an increase in perfusate 2,5-dihydroxybenzoic acid consistent with an early post-ischemic burst of .OH, not unlike that reported using spin trapping techniques. Since then we and others have used this technique to examine possible relationships between .OH formation and treatments that alter post-ischemic cardiac functional recovery. For example, preischemic loading of hearts with copper results in increases in post-ischemic dysfunction and LDH release that were associated with an increase in 2,5-dihydroxybenzoate and by inference, .OH formation. Alternatively, we have reported that the nitroxide spin label, TEMPO, reputed to be a superoxide dismutase mimetic, decreased post-ischemic arrhythmias and 2,5-dihydroxybenzoate formation. Most recently, we have observed that preischemic loading of hearts with zinc-bis-histidinate results in improved post-ischemic cardiac function and decreased LDH release; changes that were associated with decreased 2,5-dihydroxybenzoate formation. These studies indicate that under certain conditions, salicylate is a valuable alternative to spin trapping techniques to probe the role of .OH in cardiac oxidative injury, particularly when applied to the isolated perfused heart preparation.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Powell SRdoi
10.3109/10715769409056588subject
Has Abstractpub_date
1994-11-01 00:00:00pages
355-70issue
6eissn
1071-5762issn
1029-2470journal_volume
21pub_type
杂志文章,评审abstract::Degenerative diseases such as cancer are induced by oxidative genetic damage. Antioxidants can scavenge reactive oxygen species, but to prevent disease, they must do so quickly, before the DNA bases are damaged. In the present study, a novel method was established for evaluating the potency of antioxidants employing 2...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760290019336
更新日期:2002-03-01 00:00:00
abstract::Cardiolipin (CL) is a mitochondria-specific phospholipid and is critical for maintaining the integrity of mitochondrial membrane and mitochondrial function. CL also plays an active role in mitochondria-dependent apoptosis by interacting with cytochrome c (cyt c), tBid and other important Bcl-2 proteins. The unique str...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2012.676642
更新日期:2012-08-01 00:00:00
abstract::After minimal sample preparation, two different HPLC methodologies, one based on a single gradient reversed-phase HPLC step, the other on multiple HPLC runs each optimised for specific components, were used to investigate the composition of flavonoids and phenolic acids in apple and tomato juices. The principal compon...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760000300551
更新日期:2000-06-01 00:00:00
abstract::Mammalian odorant-binding proteins (OBPs) are soluble lipocalins produced in the nasal mucosa and in other epithelial tissues of several animal species, where they are supposed to serve as scavengers for small structurally unrelated hydrophobic molecules. These would include odorants and toxic aldehydes like 4-hydroxy...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2014.910867
更新日期:2014-07-01 00:00:00
abstract::It has been reported that haem oxygenase-1 (Hmox1) induction attenuates neointimal thickening. Here we further investigated the potential mechanisms regulating this important pathological process. We revealed that histone deacetylase 2 (HDAC2) was induced following Hmox1 induction under 1% oxygen treatment and this in...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2018.1524578
更新日期:2018-10-01 00:00:00
abstract::Gastric inflammation is a highly complex biochemical protective response to cellular/tissue injury. When this process occurs in an uncontrolled manner, the result is excessive cellular/tissue damage that results chronic inflammation and destruction of normal tissue. Current evidence suggests that Helicobacter pylori (...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760000301301
更新日期:2000-12-01 00:00:00
abstract::The concentration of taurine is high in the lens. However, its function therein remains unknown. Studies from other tissues suggest that in addition to several other modes of action, it acts as an antioxidant. We therefore hypothesize that taurine may be a part of the antioxidant defense mechanisms involved in protect...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769800300221
更新日期:1998-09-01 00:00:00
abstract::Oxidative stress is associated with many cardiovascular diseases, such as hypertension and arteriosclerosis. Oxidative stress reportedly activates the L-type voltage-gated calcium channel (VDCCL) and elevates [Ca2+]i in many cells. However, how oxidative stress activates VDCCL under clinical setting and the consequenc...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2018.1556394
更新日期:2019-01-01 00:00:00
abstract::Liposomes composed of soybean phosphatidylcholine were peroxidized using the reagent sodium hypochlorite or the myeloperoxidase-hydrogen peroxide-Cl- system. Linoleic acid hydroperoxide previously prepared from linoleic acid by means of lipoxidase was incorporated into liposomes. The yield of thiobarbituric acid react...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769900300521
更新日期:1999-06-01 00:00:00
abstract::Sublytic levels (microM) of hemin destabilized RBC membrane as indicated by ghost fragmentation pattern using a laser viscodiffractometer. Furthermore, electron microscopic study shows that 5 microM of hemin induced echinocytic transformation whereas higher hemin concentration (40 microM) induced spherocytic transform...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709097838
更新日期:1997-07-01 00:00:00
abstract::Oxidatively generated damage to nucleic acids may play an important role in the pathophysiological processes of a variety of diseases. 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGsn) and 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn) are oxidatively generated products of DNA and RNA, respectively. Our previous studies have s...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1346249
更新日期:2017-06-01 00:00:00
abstract::Epidemiology studies have shown that consumption of fruits and vegetables is associated with the prevention of chronic diseases such as cancer and cardiovascular disease. Induction of cellular phase II detoxifying enzymes is associated with cancer preventive potential. Phenolsulfotransferases (PSTs) are traditionally ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500150424
更新日期:2005-08-01 00:00:00
abstract::Reactive oxygen species (ROS) are involved in the mechanism of photoaging and carcinogenesis. Skin is endowed with antioxidant enzymes including superoxide dismutases (SOD): cytosolic copper zinc SOD and mitochondrial manganese SOD. The aim of our study was to estimate the protective effect of manganese against oxidat...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769509065255
更新日期:1995-10-01 00:00:00
abstract::Adenosine levels are increased in stress and act as anti-oxidant and anti-inflammatory mediators by binding to 4 G-protein-coupled receptors. Using genetically modified mice lacking A(1) and A(3) adenosine receptors, treated with ip buthionine-[S,R]-sulphoximine injections to inhibit gamma-glutamylcysteine ligase, the...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760802712616
更新日期:2009-03-01 00:00:00
abstract::Approaching living systems, aqueous solutions are appropriate to characterize antioxidants, whereas the frequently used standard 1,1-diphenyl-2-picrylhydrazyl (DPPH) is insoluble in water. Therefore, mixed water-ethanol solvents were investigated using the electron paramagnetic resonance (EPR) spectroscopy. Two forms ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600930014
更新日期:2007-04-01 00:00:00
abstract::MCI-186 (3-methyl-1-phenyl-2-pyrazolin-5-one) is a newly developed antioxidant which has been shown to reduce brain edema in cerebral ischemia through inhibition of the lipoxygenase pathway of arachidonic acid. However, its effect on myocardial reperfusion injury after prolonged ischemia has not yet been demonstrated....
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769609088034
更新日期:1996-05-01 00:00:00
abstract::The hydroxyl radical adducts of 5,5 dimethyl-1-pyrolline-N-oxide (DMPO) and 3,3,5,5 tetramethyl-1-pyrolline-N-oxide (TMPO) formed in the presence of hydrogen peroxide and FeII are normally quite stable, but in the presence of 5-20 micromolar myoglobin their ESR signals decay rapidly. This decay probably reflects furth...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769409145632
更新日期:1994-05-01 00:00:00
abstract::8-OH-deoxyguanosine can diminish the ability of the restriction endonucleases Hpa II and Msp I to cleave DNA. The exact position of the adduct within the recognition site appears to determine the extent of the effect. ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769509064038
更新日期:1995-09-01 00:00:00
abstract::The mechanism of high-grade serous ovarian cancer (HGSC) development remains elusive. This review outlines recent advances in the understanding of sequential molecular changes associated with the development of HGSC, as well as describes oxidative stress-induced genomic instability and carcinogenesis. This article rev...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715762.2017.1383605
更新日期:2017-10-01 00:00:00
abstract::It is well known that reactive oxygen species (ROS) attack several living tissues and increase the risk of development and progression of serious diseases. In neuronal level, ROS induce cell death in concentration-dependent fashion. However, little is known about the mechanisms of neuronal changes by ROS prior to indu...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2011.647689
更新日期:2012-02-01 00:00:00
abstract::The beneficial effects of hyperoxia have been noted in treatment of several diseases and pathological states. However, the excessive production of ROS under hyperoxic conditions can directly damage cellular macromolecules if the imbalance in antioxidant status exists. Cytochrome P450 (Cyp) 4a14 has an important role i...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715760903390820
更新日期:2010-02-01 00:00:00
abstract::Polystyrene supported fluorinated cyclic nitrone spin-traps: Resin-2-HFDMPO (2-hydroxymethyl-2-methyl-5-(trifluoromethyl)-3,4-dihydro-2H-pyrrole-1-oxide) and Resin-2-PFDMPO (2-(3-hydroxypropyl)-2-methyl-5-(trifluoromethyl)-3,4-dihydro-2H-pyrrole 1-oxide) containing a trifluoromethyl pyrroline-N-oxide core were develop...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2019.1683171
更新日期:2019-12-01 00:00:00
abstract::We have determined the effects of chronic vitamin C intake on neutrophil and lymphocyte antioxidant defences during the acute phase immune response induced by intense exercise. Blood samples were taken from 16 voluntary athletes in basal conditions, both immediately after and 1 h after a duathlon competition. Sportsme...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576031000150454
更新日期:2003-09-01 00:00:00
abstract::A glycomic approach to the identification of target molecules in glycosyltransferase gene targeting mice is a promising strategy to understand the biological significance of glycosyltransferase genes in vivo. In order to understand the biological effects of N-acetylglucosaminyltransferase III (GnT-III) on tumor format...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576021000005258
更新日期:2002-08-01 00:00:00
abstract::3-Nitrotyrosine (3NT) is known as an important indicator of nitrosative stress and has been linked to various diseases. Our aim was to develop an indirect ELISA (enzyme-linked immunosorbent assay) method suitable for the detection of protein-bound 3NT in clinical plasma and serum samples. Nitrated protein standards an...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2011.652627
更新日期:2012-03-01 00:00:00
abstract::Diosgenin possesses anti-inflammatory and anticancer properties. Activated neutrophils produce high concentrations of the superoxide anion which is involved in the pathophysiology of inflammation-related diseases and cancer. In the present study, the inhibitory effect and possible mechanisms of diosgenin on superoxide...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2014.966705
更新日期:2014-12-01 00:00:00
abstract::Resorcinols (pentadecylresorcinol, olivetol, orcinol and resorcinol) exhibit antioxidant properties in liposomal systems. Antioxidant potency depends on the length of the alkyl chain. Pentadecylresorcinol has been demonstrated to be the most active antioxidant, indicating significance of its alkyl chain in a lipid bil...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769809070804
更新日期:1998-04-01 00:00:00
abstract::The purpose of the present study was to investigate the protective effect of esculetin (ES) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the lung epithelial A549 cells. Mice were intragastrically administered with ES (20 and 40 mg/kg) 1 h prior to LPS challenge. ES pretreatment at doses of 20 and 40...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1087643
更新日期:2015-01-01 00:00:00
abstract::Microcystin-LR (MCLR) is a potent hepatotoxin. Oxidative stress is thought to be implicated in the cytotoxicity of MCLR, but the mechanisms by which MCLR produces reactive oxygen species (ROS) are still unclear. This study investigated the role and possible sources of ROS generation in MCLR-induced cytogenotoxicity in...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701704599
更新日期:2007-12-01 00:00:00
abstract::We investigated the effects of fluvastatin, a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, on reactive oxygen species (ROS) and on oxidative DNA damage in vitro, as well as the effects of the main fluvastatin metabolites (M2, M3, and M4) and other inhibitors of the same enzyme, pravastatin and simvastati...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100301291
更新日期:2001-12-01 00:00:00