Abstract:
:Oxygen radical generating systems, namely, Cu(II)/ H2O2, Cu(II)/ascorbate, Cu(II)/NAD(P)H, Cu(II)/ H2O2/catecholamine and Cu(II)/H2O2/SH-compounds irreversibly inhibited yeast glutathione reductase (GR) but Cu(II)/H2O2 enhanced the enzyme diaphorase activity. The time course of GR inactivation by Cu(II)/H2O2 dependent on Cu(II) and H2O2 concentrations and was relatively slow, as compared with the effect of Cu(II)/ascorbate. The fluorescence of the enzyme Tyr and Trp residues was modified as a result of oxidative damage. Copper chelators, catalase, bovine serum albumin and HO. scavengers prevented GR inactivation by Cu(II)/H2O2 and related systems. Cysteine, N-acetylcysteine, N-(2-dimercaptopropionylglycine and penicillamine enhanced the effect of Cu(II)/H2O2 in a concentration- and time-dependent manner. GSH, Captopril, dihydrolipoic acid and dithiotreitol also enhanced the Cu(II)/H2O2 effect, their actions involving the simultaneous operation of pro-oxidant and antioxidant reactions. GSSG and trypanothione disulfide effectively protected GR against Cu(II)/H2O2 inactivation. Thiol compounds prevented GR inactivation by the radical cation ABTS.+. GR inactivation by the systems assayed correlated with their capability for HO. radical generation. The role of amino acid residues at GR active site as targets for oxygen radicals is discussed.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Gutierrez-Correa J,Stoppani AOdoi
10.3109/10715769709097858subject
Has Abstractpub_date
1997-12-01 00:00:00pages
543-55issue
6eissn
1071-5762issn
1029-2470journal_volume
27pub_type
杂志文章,评审abstract::The response of HeLa cells to subcellular spatially localized two-photon irradiation of a singlet oxygen photosensitizer (protoporphyrin IX, PpIX) using a focused laser was assessed. Upon irradiation under these conditions, a localized population of PpIX excited states can be produced with meaningful intracellular spa...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2013.817670
更新日期:2013-09-01 00:00:00
abstract::Of all the aerobic respiration by-products, cytotoxic superoxide derived from mitochondrial-leaked electrons, is the only one known to be disposed of intracellularly. Is this fate the only destiny for mitochondrial-leaked electrons? When Cynomolgus monkeys were injected intravenously with reactive oxygen species (ROS)...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2019.1677902
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND:Although cis-diamminedichloroplatinum (II) (cisplatin) is an effective anticancer agent, its clinical use is highly limited predominantly due to its adverse effects on renal functions. The present work examined the therapeutic potential of edaravone, a free radical scavenger, for inhibiting cisplatin-induced...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760310001646886
更新日期:2004-04-01 00:00:00
abstract::To examine the involvement of p38 mitogen-activated protein kinase (p38 MAPK) and extracellular signal-regulated kinase (ERK) in the oxidative stress-induced increase of permeability in endothelial cells, the effects of a p38 MAPK inhibitor (SB203580) and ERK inhibitor (PD90859) on the H2O2-induced increase of permeab...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100301531
更新日期:2001-11-01 00:00:00
abstract::This work describes the synthesis and characterisation of some novel hybrid molecules which contains in the same molecule a free radical moiety of hydrazyl type and a spin-trap moiety of nitrone type. The new compounds synthesized have multiple and easy to follow spectroscopic properties, making them useful as sensors...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500385699
更新日期:2006-01-01 00:00:00
abstract::To solve the problems faced in clinical use of hypocrellins, a water-soluble preparation of Hypocrellin B (HB), HB-Triton X-100 (TX-100) micelles, was prepared. To evaluate the photodynamic activity, the free radicals (*OH and HB*-) and singlet oxygen (1O2) generated via photosensitization of the preparation in aqueou...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760310001601056
更新日期:2003-10-01 00:00:00
abstract::Gamma-glutamyltransferase (GGT) plays a central role in the homeostasis of the antioxidant glutathione (GSH). The expression of GGT has been shown to be upregulated after oxidative stress, but the signalling pathways implicated remain poorly characterized. The results here show that acute exposure of CC531 cells to ox...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701739488
更新日期:2007-12-01 00:00:00
abstract::Involvement of oxidative stress is implicated in the progression of complication of diabetes mellitus. With respect to heart diseases, we have studied role of oxidative stress/antioxidants using rats treated with streptozotocin to induce diabetes (DM). Hemodynamic and echocardiographic measurements showed thickening o...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100300231
更新日期:2001-03-01 00:00:00
abstract::The oxidized intermediates generated upon exposure of Aspergillus niger catalase to hydrogen peroxide and superoxide radical fluxes were examined with UV-visible spectrophotometry. Hydrogen peroxide and superoxide radical were generated by means of glucose/glucose oxidase and xanthine/xanthine oxidase systems. Serial ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769409145624
更新日期:1994-01-01 00:00:00
abstract::Weaning mice were fed a diet supplemented with beef tallow (BT) or BT plus docosahexaenoic acid (DHA) containing 100 mg alpha-tocopherol/kg (alpha-Toc100) or 500 mg alpha-tocopherol/kg (alpha-Toc500) for 4 wk to modify membrane fatty acid unsaturation, and then were administered ferric nitrilotriacetate (Fe-NTA). The ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769900300221
更新日期:1999-03-01 00:00:00
abstract::Lipid peroxidation (LPO) product accumulation in human tissues is a major cause of tissular and cellular dysfunction that plays a major role in ageing and most age-related and oxidative stress-related diseases. The current evidence for the implication of LPO in pathological processes is discussed in this review. New d...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2010.498478
更新日期:2010-10-01 00:00:00
abstract::Interactions between transition metal ions and polyphenols can result in complexation, redox or polymerization, but the relative importance of these reactions is unclear. The present paper reports results from the reaction of gallic acid (GA) with Cu(II) using electron paramagnetic resonance (EPR) and UV/visible spect...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2010.515220
更新日期:2011-02-01 00:00:00
abstract::To determine effect of nitric oxide (NO) on cellular glutathione peroxidase (GPX) level in living cells, we measured the activity, protein and mRNA of GPX in rat kidney (KNRK) cells under a high NO condition. Combined treatment of lipopolysaccharide (LPS, 1 microgram/ml) and tumor necrosis factor-alpha (TNF-alpha, 50 ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100300851
更新日期:2001-09-01 00:00:00
abstract::Adriamycin (ADR) is a chemotherapeutic agent useful in treating various cancers. ADR is a quinone-containing anthracycline chemotherapeutic and is known to produce reactive oxygen species (ROS) in heart. Application of this drug can have serious side effects in various tissues, including brain, apart from the known ca...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500143478
更新日期:2005-11-01 00:00:00
abstract::The present study has been designed and carried out to investigate the protective role of taurine (2-aminoethanesulphonic acid) against NaAsO(2) induced nephrotoxicity. Oral administration of arsenic increased the productions of ROS and RNS, enhanced lipid peroxidation, protein carbonylation and decreased intracellula...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760903164998
更新日期:2009-10-01 00:00:00
abstract::Methylmercury (MeHg) is one of the ubiquitous environmental toxicant that leads to long-lasting neurological deficits in animals and humans. However, the mechanisms of MeHg-induced neuronal cell death are incompletely understood. Treatment of neuronal cells with MeHg (0-2 μM) for 0.5-12 h, or pretreated with LA (12.5-...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2016.1152362
更新日期:2016-01-01 00:00:00
abstract::It has been observed that H9c2 cardiac cells cultured in physiologic solutions exhibit delayed cell death after repeated medium replacements, of which the cause was the relatively mild osmotic challenges during the renewal of the culture medium. Interestingly, the cell damage was associated with altered intracellular ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760802691471
更新日期:2009-03-01 00:00:00
abstract::The significance of 5-lipoxygenase and myeloperoxidase activities has not been extensively studied among young male smokers. Leukotriene B(4), 20-hydroxy-leukotriene B(4), 20-carboxy-leukotriene B(4) and 3-chlorotyrosine were measured in plasma and urinary samples of young male smokers at 8 hours following cigarette a...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2012.701291
更新日期:2012-10-01 00:00:00
abstract::It has previously been reported that the globular form of adiponectin (gAd), mature adipocyte-derived cytokine, induced generation of reactive oxygen species (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. This study investigated whether diacylglycerol kinases (DGKs), enzymes functioning in sub...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2010.532495
更新日期:2011-03-01 00:00:00
abstract::Mammalian odorant-binding proteins (OBPs) are soluble lipocalins produced in the nasal mucosa and in other epithelial tissues of several animal species, where they are supposed to serve as scavengers for small structurally unrelated hydrophobic molecules. These would include odorants and toxic aldehydes like 4-hydroxy...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2014.910867
更新日期:2014-07-01 00:00:00
abstract::In this study, we studied whether chronic oral administration of the natural antioxidant, malabaricone C (mal C) can reduce blood pressure (BP) and attenuate cardio-vascular remodeling in deoxycorticosterone acetate (DOCA)-salt hypertensive rats. The dose of mal C for its anti-hypertensive action was optimized by meas...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1112005
更新日期:2016-01-01 00:00:00
abstract::Oxidative stress appears to be implicated in the pathogenesis of various diseases including hepatotoxicity. Although intracellular Ca2+ signals have been suggested to play a role in the oxidative damage of hepatocytes, the sources and effects of oxidant-induced intracellular Ca2+ increases are currently debatable. Thu...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760000301431
更新日期:2000-09-01 00:00:00
abstract::The effect of antioxidants on ammonia induced CNS and renal pathobiology in 10 sheep infested by L. Cuprina larvae was investigated. The condition produces severe dermatitis, proliferation of macrophages and hyperammonaemia, and free radicals may therefore be involved in the pathogenesis. Five of the sheep (treated gr...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769409056553
更新日期:1994-07-01 00:00:00
abstract::Using a novel phosphorylated spin trap, 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO), an analogue of the commonly used spin trap 5,5'-dimethyl-1-pyrroline N-oxide (DMPO), we have investigated the production of oxygen radical species under illumination of thylakoids from wheat (Triticum durum Desf cv. O...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769900301511
更新日期:1999-12-01 00:00:00
abstract::Oxidative stress is associated with a range of inflammatory lung diseases including asthma, adult respiratory distress syndrome, idiopathic pulmonary fibrosis, pneumonia, lung transplantation, chronic obstructive pulmonary disease, cystic fibrosis, bronchiectasis and lung cancer. Increased concentrations of reactive o...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760310001623331
更新日期:2003-12-01 00:00:00
abstract::Autophagy modulation is a potential therapeutic strategy for breast cancer, and a previous study indicated that metformin exhibits significant anti-carcinogenic activity. However, the ability of metformin to induce autophagy and its role in breast cancer cell death remains unclear. In this study, we exposed MCF-7 cell...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2018.1485075
更新日期:2018-08-01 00:00:00
abstract::5-(2,2-dimethyl-1,3-propoxy cyclophosphoryl)-5-methyl-1-pyrroline N-oxide (CYPMPO), a new cyclic DEPMPO-type nitrone was evaluated for spin-trapping capabilities toward hydroxyl and superoxide radicals. CYPMPO is colorless crystalline and freely soluble in water. Both the solid and diluted aqueous solution did not dev...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600883254
更新日期:2006-11-01 00:00:00
abstract::The synproportionation reaction between ferryl leghemoglobin and oxyleghemoglobin does not occur, at least under conditions where this process could be clearly demonstrated with myoglobin and hemoglobin. In contrast, a cross synproportionation can occur between oxyleghemoglobin and ferryl myoglobin or between ferryl l...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709097848
更新日期:1997-08-01 00:00:00
abstract::Approaching living systems, aqueous solutions are appropriate to characterize antioxidants, whereas the frequently used standard 1,1-diphenyl-2-picrylhydrazyl (DPPH) is insoluble in water. Therefore, mixed water-ethanol solvents were investigated using the electron paramagnetic resonance (EPR) spectroscopy. Two forms ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600930014
更新日期:2007-04-01 00:00:00
abstract::Intracellular levels of H2O2 in BHK-21 cells are not static but decline progressively with cell growth. Exposure of cells to inhibitors of catalase, or glutathione peroxidase, not only diminishes this decline but also depresses rates of cell proliferation, suggesting important growth regulatory roles for those antioxi...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769509065268
更新日期:1995-11-01 00:00:00