Abstract:
:The response of HeLa cells to subcellular spatially localized two-photon irradiation of a singlet oxygen photosensitizer (protoporphyrin IX, PpIX) using a focused laser was assessed. Upon irradiation under these conditions, a localized population of PpIX excited states can be produced with meaningful intracellular spatial resolution; the dimensions of the domain where the incident light flux is high enough for PpIX two-photon absorption are defined by the microscope optics and by the diffraction of light (spot diameter at beam waist of ˜0.5-1.0 μm). In turn, the dimensions of the intracellular domain containing cytotoxic PpIX-sensitized singlet oxygen will likewise be confined. Most importantly, cell response (e.g., morphological signs of cell death) correlates with the light dose delivered and the intracellular domain irradiated. Thus, controlling light delivery can complement other techniques used to impart intracellular spatial localization in mechanistic studies of photoinitiated reactive oxygen species. Such controlled light delivery is also expected to be a particularly useful tool to study the so-called bystander effect in which a selectively-perturbed cell can influence a neighboring cell through intercellular signaling mechanisms.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Gollmer A,Besostri F,Breitenbach T,Ogilby PRdoi
10.3109/10715762.2013.817670subject
Has Abstractpub_date
2013-09-01 00:00:00pages
718-30issue
9eissn
1071-5762issn
1029-2470journal_volume
47pub_type
杂志文章abstract::Hypericin and pseudohypericin are polycyclic-phenolic structurally related compounds found in Hypericum perforatum L. (St John's wort). As hypericin has been found to bind to LDL one may assume that it can act as antioxidant of LDL lipid oxidation, a property which is of prophylactic/therapeutic interest regarding ath...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600978831
更新日期:2007-02-01 00:00:00
abstract::Endothelial dysfunction is an early pathophysiological feature and independent predictor of poor prognosis in most forms of cardiovascular diseases. Epidemiological studies report an inverse association between dietary flavonoid consumption and mortality from cardiovascular diseases. In the present paper, we review th...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760600823128
更新日期:2006-10-01 00:00:00
abstract::p38 MAPKs are important mediators of signal transduction that respond to a wide range of extracellular stressors such as UV radiation, osmotic shock, hypoxia, pro-inflammatory cytokines, and oxidative stress. The most abundant family member is p38α, which helps to couple cell proliferation and growth in response to ce...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2013.821200
更新日期:2013-11-01 00:00:00
abstract::Expression of multiple drug resistant (MDR) phenotype and over-expression of P-glycoprotein (P-gp) in the human hepatocellular carcinoma (HCC) cell clone P1(0.5), derived from the PLC/PRF/5 cell line (P5), are associated with strong resistance to oxidative stress and a significant (p < 0.01) increase in intracellular ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760410001705140
更新日期:2004-07-01 00:00:00
abstract::Thioredoxin-1 (TRX) is a redox-active protein with anti-inflammatory effects. This study investigated the optimal delivery method and the mechanisms of recombinant human TRX (rhTRX) to suppress neutrophil recruitment in a rat bleomycin (BLM)-induced sustained acute lung injury model. In male Wister rats intratracheall...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701487682
更新日期:2007-10-01 00:00:00
abstract::Reperfusion injury of the liver occurs in liver transplantation and in major hepatectomies. It triggers a severe oxidative stress that leads to increased lipid peroxidation. In our study we examined the effect of parenteral supranutritional administration of alpha-tocopherol, a vitamin that plays a key role in the end...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100300641
更新日期:2001-08-01 00:00:00
abstract::APE1/Ref-1, the mammalian ortholog of E. coli Xth, and a multifunctional protein possessing both DNA repair and transcriptional regulatory activities, has dual role in controlling cellular response to oxidative stress. It is rate-limiting in repair of oxidative DNA damage including strand breaks and also has co-transc...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701765616
更新日期:2008-01-01 00:00:00
abstract::Oxidative DNA modification has been implicated in development of certain cancers and 8-oxodG, the most abundant and mutagenic DNA modification, has for some time been considered a biomarker of this activity. Urinary excretion of 8-oxodG over 24h has been used to estimate the rate of damage to DNA, and animal studies h...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769800300601
更新日期:1998-12-01 00:00:00
abstract::Silibinin is an active constituent extracted from blessed milk thistle (Silybum marianum). Our previous study demonstrated that silibinin induced autophagy and apoptosis via reactive oxygen species (ROS) generation in HeLa cells. In this study, we investigated whether the autophagy- and apoptosis-associated molecules ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2012.655244
更新日期:2012-03-01 00:00:00
abstract::Generation of toxic oxygen metabolites followed by oxidant- and inflammatory-mediated tissue injury plays a crucial role in the pathogenesis of ischemia and reperfusion (IR). Ubiquinol, the reduced form of coenzyme Q10, is recognized for its potent antioxidant and anti-inflammatory properties in biological membranes. ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2013.858148
更新日期:2014-02-01 00:00:00
abstract::Epidemiologically, a high-carotenoid intake via a fruit- and vegetable-rich diet is associated with a decreased risk of various forms of cancer. The mechanisms by which carotenoids exert this protective effect are controversial. In this study, we examined the potency of a range of carotenoids commonly found in human p...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760290032539
更新日期:2002-07-01 00:00:00
abstract::The intracellular superoxide and glutathione disulphide concentrations increased in Penicillium chrysogeum treated with 50, 250 or 500 microM menadione (MQ). A significant increase in the intracellular peroxide concentration was also observed when mycelia were exposed to 250 or 500 microM MQ. The specific activity of ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769900300131
更新日期:1999-02-01 00:00:00
abstract::Experimental autoimmune encephalomyelitis (EAE) reproduces a multiple sclerosis (MS)-like experimental model. The main objective was to evaluate the effect of extremely low-frequency electromagnetic fields (EL-EMF) application, like a paradigm of transcranial magnetic stimulation (TMS) in the development of EAE. Rats ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1324955
更新日期:2017-05-01 00:00:00
abstract::MCI-186 (3-methyl-1-phenyl-2-pyrazolin-5-one) is a newly developed antioxidant which has been shown to reduce brain edema in cerebral ischemia through inhibition of the lipoxygenase pathway of arachidonic acid. However, its effect on myocardial reperfusion injury after prolonged ischemia has not yet been demonstrated....
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769609088034
更新日期:1996-05-01 00:00:00
abstract::Tropolones, the naturally occurring compounds responsible for the durability of heartwood of several cupressaceous trees, have been shown to possess both metal chelating and antioxidant properties. However, little is known about the ability of tropolone and its derivatives to protect cultured cells from oxidative stre...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760400017244
更新日期:2005-02-01 00:00:00
abstract::The host inflammatory response appears to be an important contributor to the pathogenesis of human viral respiratory illness. Virus-induced oxidative stress appears to mediate an early phase of elaboration of the proinflammatory cytokine interleukin-8 by respiratory epithelial cells. The purpose of these studies was t...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576021000028316
更新日期:2002-10-01 00:00:00
abstract::Stichodactyla heliantus II (St II) is a haemolytic toxin whose activity depends of the characteristics of red blood cells (RBC). Among the factors that may tune the response of the RBC to the toxin activity stand the oxidative status of the cell. This study investigates how pre-oxidation of RBC modifies St II activity...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2010.536838
更新日期:2011-04-01 00:00:00
abstract::A glycomic approach to the identification of target molecules in glycosyltransferase gene targeting mice is a promising strategy to understand the biological significance of glycosyltransferase genes in vivo. In order to understand the biological effects of N-acetylglucosaminyltransferase III (GnT-III) on tumor format...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576021000005258
更新日期:2002-08-01 00:00:00
abstract::Globular adiponectin (gAd) induces the generation of reactive oxygen species (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. This study investigated the role of the mammalian target of rapamycin (mTOR) in gAd-induced ROS and NO generation. gAd stimulation induced phosphorylation of mTOR, which ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715760903348328
更新日期:2010-02-01 00:00:00
abstract::We have determined the effects of chronic vitamin C intake on neutrophil and lymphocyte antioxidant defences during the acute phase immune response induced by intense exercise. Blood samples were taken from 16 voluntary athletes in basal conditions, both immediately after and 1 h after a duathlon competition. Sportsme...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576031000150454
更新日期:2003-09-01 00:00:00
abstract::Hydroxyl and 1-hydroxyethyl radical adducts of 5,5-dimethylpyrroline N-oxide (DMPO) were prepared by photolysis, and mechanisms for loss of their EPR signals in rat liver microsomal suspensions were evaluated. Rates of NADPH-dependent EPR signal loss were more rapid in phosphate buffer than in Tris buffer. Addition of...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769609149069
更新日期:1996-12-01 00:00:00
abstract::In aerobes, oxygen is essential for maintenance of life. However, incomplete reduction of oxygen leads to generation of reactive oxygen species. These oxidants oxidise biological macromolecules present in their vicinity and thereby impair cellular functions causing oxidative stress (OS). Aerobes have evolved both enzy...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715762.2019.1702656
更新日期:2020-01-01 00:00:00
abstract::Degenerative diseases such as cancer are induced by oxidative genetic damage. Antioxidants can scavenge reactive oxygen species, but to prevent disease, they must do so quickly, before the DNA bases are damaged. In the present study, a novel method was established for evaluating the potency of antioxidants employing 2...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760290019336
更新日期:2002-03-01 00:00:00
abstract::The bioactive flavonoid baicalein has been shown to have radioprotective activity, although the molecular mechanism is poorly understood in vivo. C57BL/6 mice were irradiated with X-rays (15 Gy) with and without baicalein treatment (5 mg/kg/day). Irradiation groups showed an increase of NF-κB-mediated inflammatory fac...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2011.555479
更新日期:2011-05-01 00:00:00
abstract::The effects of various free radicals derived from 6-formylpterin (6-FP), alpha-phenyl-tert-butyl nitrone (PBN) and 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) combined with hyperthermia, on gene expression in similarly enhanced apoptosis of human lymphoma U937 cells were investigated using cDNA microarrays c...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600946432
更新日期:2007-01-01 00:00:00
abstract::To define the molecular mechanism(s) of carvedilol inhibition of lipid peroxidation we have utilized model systems that allow us to study the different reactions involved in this complex process. Carvedilol inhibits the peroxidation of sonicated phosphatidylcholine liposomes triggered by FeCl2 addition whereas atenolo...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769800300421
更新日期:1998-11-01 00:00:00
abstract::Oxidative stress may initiate lipid peroxidation that generates ethane. Ethane, at low concentrations, is eliminated by pulmonary exhalation. Previous methods have not allowed frequent sampling, thus ethane kinetics has not been studied in man. A validated method over the range 3.8-100,000 ppb with a limit of quantita...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576031000107353
更新日期:2003-08-01 00:00:00
abstract::Nonalcoholic fatty liver disease (NAFLD) is characterised by excessive accumulation of hepatic lipids and oxidative injury of hepatocytes. Scutellarin is a flavonoid glycoside having antioxidative stress activity. Our current study aims to investigate the molecular mechanism of scutellarin ameliorating NAFLD. Scutella...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1422602
更新日期:2018-02-01 00:00:00
abstract::Exposure to asbestos fiber is central to mesothelial carcinogenesis. Recent sequencing studies on human and rodent malignant mesothelioma (MM) revealed frequently mutated genes, including CDKN2A, BAP1 and NF2. Crocidolite directly or indirectly catalyses the generation of hydroxyl radicals, which appears to be the maj...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2020.1743285
更新日期:2020-03-01 00:00:00
abstract::Reactive oxygen species (ROS) released from polymorphonuclear leukocytes and macrophages could cause DNA damage, but also induce cell death. Therefore inhibition of cell death must be an important issue for accumulation of genetic changes in lymphoid cells in inflammatory foci. Scavengers in the post culture medium of...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760000300601
更新日期:2000-07-01 00:00:00