Effects of transcranial magnetic stimulation on oxidative stress in experimental autoimmune encephalomyelitis.


:Experimental autoimmune encephalomyelitis (EAE) reproduces a multiple sclerosis (MS)-like experimental model. The main objective was to evaluate the effect of extremely low-frequency electromagnetic fields (EL-EMF) application, like a paradigm of transcranial magnetic stimulation (TMS) in the development of EAE. Rats were injected with a single dose of 150 μg of myelin oligodendrocyte glycoprotein (MOG, fragment 35-55) to produce experimental MS. To assess the effect of TMS application in EAE, the rats were treated with TMS (60 Hz and 0.7 mT) for 2 h in the morning, once a day, 5 days a week, during 3 weeks. TMS was applied to the head. The effect of TMS on EAE was evaluated as motor symptoms and, oxidative and cell damage. The data showed that MOG induced motor symptoms as tail paralysis and limb paresis/paralysis, oxidative stress and cell death similar to MS when compared with control animals. Importantly, TMS application attenuated motor symptoms, oxidative and cell damage, whereas it increased antioxidant system. Our findings suggest that: (i) MOG reproduces an experimental model of MS characterised by oxidative and cell damage; and (ii) TMS application decreases oxidative stress and cell death induced by MOG.


Free Radic Res


Free radical research


Medina-Fernandez FJ,Escribano BM,Agüera E,Aguilar-Luque M,Feijoo M,Luque E,Garcia-Maceira FI,Pascual-Leone A,Drucker-Colin R,Tunez I




Has Abstract


2017-05-01 00:00:00












  • Analysis of the oxidative stress response of Penicillium chrysogenum to menadione.

    abstract::The intracellular superoxide and glutathione disulphide concentrations increased in Penicillium chrysogeum treated with 50, 250 or 500 microM menadione (MQ). A significant increase in the intracellular peroxide concentration was also observed when mycelia were exposed to 250 or 500 microM MQ. The specific activity of ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Emri T,Pócsi I,Szentirmai A

    更新日期:1999-02-01 00:00:00

  • Genes, ageing and longevity in humans: problems, advantages and perspectives.

    abstract::Many epidemiological data indicate the presence of a strong familial component of longevity that is largely determined by genetics, and a number of possible associations between longevity and allelic variants of genes have been described. A breakthrough strategy to get insight into the genetics of longevity is the stu...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Salvioli S,Olivieri F,Marchegiani F,Cardelli M,Santoro A,Bellavista E,Mishto M,Invidia L,Capri M,Valensin S,Sevini F,Cevenini E,Celani L,Lescai F,Gonos E,Caruso C,Paolisso G,De Benedictis G,Monti D,Franceschi C

    更新日期:2006-12-01 00:00:00

  • Serum total antioxidant activity in relative hypo- and hypercholesterolemia.

    abstract::Individuals with low serum cholesterol experience greater than expected age-adjusted mortality from non-atherosclerotic diseases, including cancer, respiratory and digestive illnesses, but the basis for these associations remains unclear. The current investigation considered the hypothesis that hypocholesterolemia is ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Muldoon MF,Kritchevsky SB,Evans RW,Kagan VE

    更新日期:1996-09-01 00:00:00

  • Kinetics of 3-nitrotyrosine modification on exposure to hypochlorous acid.

    abstract::The markers 3-nitrotyrosine and 3-chlorotyrosine are measured as surrogates for reactive nitrogen species and hypochlorous acid respectively, which are both elevated in inflamed human tissues. Previous studies reported a loss of 3-nitrotyrosine when exposed to hypochlorous acid, suggesting that observations of 3-nitro...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Curtis MP,Neidigh JW

    更新日期:2014-11-01 00:00:00

  • Spatially resolved two-photon irradiation of an intracellular singlet oxygen photosensitizer: correlating cell response to the site of localized irradiation.

    abstract::The response of HeLa cells to subcellular spatially localized two-photon irradiation of a singlet oxygen photosensitizer (protoporphyrin IX, PpIX) using a focused laser was assessed. Upon irradiation under these conditions, a localized population of PpIX excited states can be produced with meaningful intracellular spa...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Gollmer A,Besostri F,Breitenbach T,Ogilby PR

    更新日期:2013-09-01 00:00:00

  • Invited review: manganese superoxide dismutase in disease.

    abstract::Manganese superoxide dismutase (MnSOD) is essential for life as dramatically illustrated by the neonatal lethality of mice that are deficient in MnSOD. In addition, mice expressing only 50% of the normal compliment of MnSOD demonstrate increased susceptibility to oxidative stress and severe mitochondrial dysfunction r...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Macmillan-Crow LA,Cruthirds DL

    更新日期:2001-04-01 00:00:00

  • Oxidative stress to human lymphocytes by xanthine oxidoreductase activity.

    abstract::The in vitro toxicity of the reactive oxygen species generating enzyme xanthine oxidoreductase (XOR) to human peripheral blood lymphocytes was studied after stimulation with phytohaemoagglutinin or anti-CD3/CD28 antibodies. Apoptosis and necrosis were induced by the XOR/hypoxanthine system in a time- and concentration...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Battelli MG,Musiani S,Tazzari PL,Stirpe F

    更新日期:2001-12-01 00:00:00

  • Low oxygen tension alleviates oxidative damage and delays cellular senescence in G6PD-deficient cells.

    abstract::Previous studies have shown that glucose-6-phosphate dehydrogenase (G6PD)-deficient cells are under increased oxidative stress and undergo premature cellular senescence. The present study demonstrates that G6PD-deficient cells cultured under 3% oxygen concentration had an extended replicative lifespan, as compared wit...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Ho HY,Cheng ML,Cheng PF,Chiu DT

    更新日期:2007-05-01 00:00:00

  • The effect of myoglobin on the stability of the hydroxyl-radical adducts of 5,5 dimethyl-1-pyrolline-N-oxide (DMPO), 3,3,5,5 tetramethyl-1-pyrolline-N-oxide (TMPO) and 1-alpha-phenyl-tert-butyl nitrone (PBN) in the presence of hydrogen peroxide.

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    journal_title:Free radical research

    pub_type: 杂志文章


    authors: De Bono D,Yang WD,Symons MC

    更新日期:1994-05-01 00:00:00

  • EPR and electrochemical quantification of oxygen using newly synthesized para-silylated triarylmethyl radicals.

    abstract::Novel silylated triarylmethyl (TAM) radicals based on TAM core CT-03 and their electron paramagnetic resonance (EPR) spectra are evaluated as a function of oxygen concentration. Combination of peak-to-peak linewidth of the EPR signal and electrochemical determination allows designing a method for oxygen quantification...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Boutier-Pischon A,Auger F,Noël JM,Almario A,Frapart YM

    更新日期:2015-03-01 00:00:00

  • Carotenoids induce apoptosis in the T-lymphoblast cell line Jurkat E6.1.

    abstract::Epidemiologically, a high-carotenoid intake via a fruit- and vegetable-rich diet is associated with a decreased risk of various forms of cancer. The mechanisms by which carotenoids exert this protective effect are controversial. In this study, we examined the potency of a range of carotenoids commonly found in human p...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Müller K,Carpenter KL,Challis IR,Skepper JN,Arends MJ

    更新日期:2002-07-01 00:00:00

  • Sequential molecular changes and dynamic oxidative stress in high-grade serous ovarian carcinogenesis.

    abstract::The mechanism of high-grade serous ovarian cancer (HGSC) development remains elusive. This review outlines recent advances in the understanding of sequential molecular changes associated with the development of HGSC, as well as describes oxidative stress-induced genomic instability and carcinogenesis. This article rev...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Kobayashi H,Ogawa K,Kawahara N,Iwai K,Niiro E,Morioka S,Yamada Y

    更新日期:2017-10-01 00:00:00

  • Inhibition of cell growth by overexpression of manganese superoxide dismutase (MnSOD) in human pancreatic carcinoma.

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    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Ough M,Lewis A,Zhang Y,Hinkhouse MM,Ritchie JM,Oberley LW,Cullen JJ

    更新日期:2004-11-01 00:00:00

  • Vitamin A deficiency induces prooxidant environment and inflammation in rat aorta.

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    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Gatica L,Alvarez S,Gomez N,Zago MP,Oteiza P,Oliveros L,Gimenez MS

    更新日期:2005-06-01 00:00:00

  • Evidence for time-dependent maximum increase of free radical damage and eicosanoid formation in the brain as related to duration of cardiac arrest and cardio-pulmonary resuscitation.

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    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Basu S,Liu X,Nozari A,Rubertsson S,Miclescu A,Wiklund L

    更新日期:2003-03-01 00:00:00

  • Role of thiocyanate, bromide and hypobromous acid in hydrogen peroxide-induced apoptosis.

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    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Wagner BA,Reszka KJ,McCormick ML,Britigan BE,Evig CB,Burns CP

    更新日期:2004-02-01 00:00:00

  • Excess processing of oxidative damaged bases causes hypersensitivity to oxidative stress and low dose rate irradiation.

    abstract::Ionizing radiations such as X-ray and γ-ray can directly or indirectly produce clustered or multiple damages in DNA. Previous studies have reported that overexpression of DNA glycosylases in Escherichia coli (E. coli) and human lymphoblast cells caused increased sensitivity to γ-ray and X-ray irradiation. However, the...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Yoshikawa Y,Yamasaki A,Takatori K,Suzuki M,Kobayashi J,Takao M,Zhang-Akiyama QM

    更新日期:2015-10-01 00:00:00

  • Effects of hydrogen peroxide on relaxation through the NO/sGC/cGMP pathway in isolated rat iliac arteries.

    abstract::The production of reactive oxygen species, including hydrogen peroxide (H(2)O(2)), is increased in diseased blood vessels. Although H(2)O(2) leads to impairment of the nitric oxide (NO)/soluble guanylate cyclase (sGC)/cGMP signaling pathway, it is not clear whether this reactive molecule affects the redox state of sGC...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Tawa M,Shimosato T,Iwasaki H,Imamura T,Okamura T

    更新日期:2015-01-01 00:00:00

  • Plasma reactive carbonyl species: Potential risk factor for hypertension.

    abstract::To study the role of oxidative stress in hypertension and pre-hypertension, this study analysed plasma levels of reactive carbonyl species (RCS) in 1204 Chinese Han adults. Results showed a statistically significant positive correlation (p < 0.001) between blood pressure and plasma RCS levels with or without being adj...

    journal_title:Free radical research

    pub_type: 杂志文章


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    更新日期:2011-05-01 00:00:00

  • Will the 'good fairies' please prove to us that vitamin E lessens human degenerative disease?

    abstract::Recent research about the role of free radical derivatives of oxygen and nitrogen in biological systems has highlighted the possibility that antioxidants, such as vitamin E, that prevent these processes in vitro may be capable of carrying out a similar function in living organisms in vivo. There is increasing evidence...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Diplock AT

    更新日期:1997-06-01 00:00:00

  • The role of endothelial dysfunction and oxidative stress in cerebrovascular diseases.

    abstract::Cerebrovascular diseases (CBD) are one of the most dangerous complications of atherosclerosis. The clinical consequences of CBD deeply impact quality of life and the prognosis of patients. Atherosclerosis is the main cause of CBD development. Hypertension, dyslipidemia, diabetes, smoking, obesity, and other risk facto...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Scicchitano P,Cortese F,Gesualdo M,De Palo M,Massari F,Giordano P,Ciccone MM

    更新日期:2019-06-01 00:00:00

  • Cyanidin-3-O-beta-glucopyranoside protects myocardium and erythrocytes from oxygen radical-mediated damages.

    abstract::The cyanidin-3-O-beta-glucopyranoside (C-3-G) antioxidant capacity towards reactive oxygen species (ROS)-mediated damages was assessed in tissue and cells submitted to increased oxidative stress. In the isolated ischemic and reperfused rat heart, 10 or 30 degreesM C-3-G protected from both lipid peroxidation (66.7 and...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Amorini AM,Lazzarino G,Galvano F,Fazzina G,Tavazzi B,Galvano G

    更新日期:2003-04-01 00:00:00

  • Intracellular redox state as determinant for melatonin antiproliferative vs cytotoxic effects in cancer cells.

    abstract::Melatonin is an endogenous indolamine, classically known as a light/dark regulator. Besides classical functions, melatonin has also showed to have a wide range of antitumoral effects in numerous cancer experimental models. However, no definite mechanism has been described to explain the whole range of antineoplasic ef...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Sánchez-Sánchez AM,Martín V,García-Santos G,Rodríguez-Blanco J,Casado-Zapico S,Suarez-Garnacho S,Antolín I,Rodriguez C

    更新日期:2011-11-01 00:00:00

  • The influence of antioxidants in the thiyl radical induced lipid peroxidation and geometrical isomerization in micelles of linoleic acid.

    abstract::The biomimetic model of micelles of linoleic acid containing 2-mercaptoethanol and the antioxidant was examined under gamma irradiation up to 400 Gy in aerobic or deoxygenated conditions where thiyl radicals are the main reactive species. Lipid peroxidation was retarded by ascorbic acid and α-tocopherol, whereas this ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Tartaro Bujak I,Mihaljević B,Ferreri C,Chatgilialoglu C

    更新日期:2016-11-01 00:00:00

  • Drugs with susceptible sites for free radical induced oxidative transformations: the case of a penicillin.

    abstract::Penicillins, as bactericidal antibiotics, have been widely used to treat infections for several decades. Their structure contains both aromatic and thioether moieties susceptible to free radical oxidation. The (•)OH induced oxidation mechanism of amoxicillin was investigated by pulse radiolysis techniques and by final...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Szabó L,Tóth T,Rácz G,Takács E,Wojnárovits L

    更新日期:2016-01-01 00:00:00

  • p38 MAPK: a dual role in hepatocyte proliferation through reactive oxygen species.

    abstract::p38 MAPKs are important mediators of signal transduction that respond to a wide range of extracellular stressors such as UV radiation, osmotic shock, hypoxia, pro-inflammatory cytokines, and oxidative stress. The most abundant family member is p38α, which helps to couple cell proliferation and growth in response to ce...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Tormos AM,Taléns-Visconti R,Nebreda AR,Sastre J

    更新日期:2013-11-01 00:00:00

  • EPR spin trapping study of the decomposition of azo compounds in aqueous solutions by ultrasound: potential for use as sonodynamic sensitizers for cell killing.

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    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Misík V,Miyoshi N,Riesz P

    更新日期:1996-07-01 00:00:00

  • Induction of glutathione peroxidase in response to inactivation by nitric oxide.

    abstract::To determine effect of nitric oxide (NO) on cellular glutathione peroxidase (GPX) level in living cells, we measured the activity, protein and mRNA of GPX in rat kidney (KNRK) cells under a high NO condition. Combined treatment of lipopolysaccharide (LPS, 1 microgram/ml) and tumor necrosis factor-alpha (TNF-alpha, 50 ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Dobashi K,Asayama K,Nakane T,Kodera K,Hayashibe H,Nakazawa S

    更新日期:2001-09-01 00:00:00

  • Regulation of gamma-glutamylcysteine synthetase subunit gene expression: insights into transcriptional control of antioxidant defenses.

    abstract::Gamma-glutamylcysteine synthetase (GCS; also referred to as glutamate-cysteine ligase, GLCL) catalyzes the rate-limiting reaction in glutathione (GSH) biosynthesis. The GCS holoenzyme is composed of a catalytic and regulatory subunit, each encoded by a unique gene. In addition to some conditions which specifically upr...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Wild AC,Mulcahy RT

    更新日期:2000-04-01 00:00:00

  • Do mitochondria make nitric oxide? no?

    abstract::Several papers have claimed that mitochondria contain nitric oxide synthase (NOS) and make nitric oxide (NO*) in amounts sufficient to affect mitochondrial respiration. However, we found that the addition of L-arginine or the NOS inhibitor L-NMMA to intact rat liver mitochondria did not have any effect on the respirat...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Tay YM,Lim KS,Sheu FS,Jenner A,Whiteman M,Wong KP,Halliwell B

    更新日期:2004-06-01 00:00:00