Effect of pre-exposure of human erythrocytes to oxidants on the haemolytic activity of Sticholysin II. A comparison between peroxynitrite and hypochlorous acid.


:Stichodactyla heliantus II (St II) is a haemolytic toxin whose activity depends of the characteristics of red blood cells (RBC). Among the factors that may tune the response of the RBC to the toxin activity stand the oxidative status of the cell. This study investigates how pre-oxidation of RBC modifies St II activity employing two oxidants, peroxynitrite and hypochlorous acid. Results show that peroxynitrite-treated RBC are more resistant to St II activity. On the other hand, hypochlorous acid-treated RBC become more susceptible to St II. This contrasting behaviour of both oxidants is related to the modifications elicited in RBC by both oxidant agents. Peroxynitrite does not modify RBC osmotic fragility but reduces anion transport through band 3 protein. This effect, together with an increase in K+ efflux, can explain the increased resistance to the toxin activity. On the other hand, results obtained with hypochlorous acid can be explained in terms of a disruption of the membrane organization without the compensating effect of a reduction in band 3-mediated anion transport. The present results, obtained employing the effect of a model haemolytic toxin on RBC, emphasize the specificity of the RBC response to different endogenous oxidative agents.


Free Radic Res


Free radical research


Celedón G,González G,Lissi E,Cerda T,Bascuñant D,Lepeley M,Pazos F,Lanio ME,Alvarez C




Has Abstract


2011-04-01 00:00:00












  • Human studies related to protein oxidation: protein carbonyl content as a marker of damage.

    abstract::Proteins constitute the major 'working force' for all forms of biological work. Their exact conformation and pattern of folding are tightly connected to their activity and function. Reactive oxygen and nitrogen species (ROS and RNS) are formed during normal metabolism and in higher fluxes under pathological conditions...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Chevion M,Berenshtein E,Stadtman ER

    更新日期:2000-11-01 00:00:00

  • Sensitization of cisplatin therapy by a naphthalimide based organoselenium compound through modulation of antioxidant enzymes and p53 mediated apoptosis.

    abstract::The widely used anti-cancer drug cisplatin imparts various toxic manifestations in the host, with nephrotoxicity being the most severe one. The trace element selenium shows antioxidant activity in both human and animals. The present study was designed to assess the chemoprotecting and chemoenhancing efficacy of a naph...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Ghosh P,Singha Roy S,Basu A,Bhattacharjee A,Bhattacharya S

    更新日期:2015-04-01 00:00:00

  • PKCδ phosphorylation is an upstream event of GSK3 inactivation-mediated ROS generation in TGF-β1-induced senescence.

    abstract::Transforming growth factor β1 (TGF-β1) induces Mv1Lu cell senescence through inactivating glycogen synthase kinase 3 (GSK3), thereby inactivating complex IV and increasing intracellular ROS. In the present study, we identified protein kinase C delta (PKCδ) as an upstream regulator of GSK3 inactivation in this mechanis...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Byun HO,Jung HJ,Kim MJ,Yoon G

    更新日期:2014-09-01 00:00:00

  • Synthesis and characterization of a practically better DEPMPO-type spin trap, 5-(2,2-dimethyl-1,3-propoxy cyclophosphoryl)-5-methyl-1-pyrroline N-oxide (CYPMPO).

    abstract::5-(2,2-dimethyl-1,3-propoxy cyclophosphoryl)-5-methyl-1-pyrroline N-oxide (CYPMPO), a new cyclic DEPMPO-type nitrone was evaluated for spin-trapping capabilities toward hydroxyl and superoxide radicals. CYPMPO is colorless crystalline and freely soluble in water. Both the solid and diluted aqueous solution did not dev...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Kamibayashi M,Oowada S,Kameda H,Okada T,Inanami O,Ohta S,Ozawa T,Makino K,Kotake Y

    更新日期:2006-11-01 00:00:00

  • Edaravone inhibits rheumatoid synovial cell proliferation and migration.

    abstract::Rheumatoid arthritis (RA) is characterized by synovial proliferation and migration which is induced by proinflammatory cytokines or oxidative stress, followed by joint destruction. Edaravone, clinically available free radical scavenger in Japan, is confirmed to be beneficial in the acute stage of cerebral infarction. ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Arii K,Kumon Y,Ikeda Y,Suehiro T,Hashimoto K

    更新日期:2006-02-01 00:00:00

  • The involvement of Nrf2 antioxidant signalling pathway in the protection of monocrotaline-induced hepatic sinusoidal obstruction syndrome in rats by (+)-catechin hydrate.

    abstract::Hepatic sinusoidal obstruction syndrome (HSOS) is a rare and life-threatening liver disease. (+)-Catechin is a natural dietary flavonol with high antioxidant capacity. This study aims to investigate the involvement of nuclear factor erythroid 2-related factor 2 (Nrf2) antioxidant signalling pathway in the protection o...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Jing X,Zhang J,Huang Z,Sheng Y,Ji L

    更新日期:2018-04-01 00:00:00

  • Relationship between serum reactive oxidative metabolite level and skin reaction in an irradiated rat model.

    abstract:PURPOSE:Ionizing radiation generates free radicals and reactive oxygen species that induce DNA damage in vivo. This study aimed to determine the relationship between serum reactive oxygen metabolite (ROM) levels and skin reaction after irradiation in a rat model. METHODS AND MATERIALS:I. Female Wistar rats were classi...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Nomiya T,Kaneko T,Goto J,Harada M,Akamatsu H,Hagiwara Y,Ota I,Nemoto K

    更新日期:2014-05-01 00:00:00

  • Lipofuscin-mediated photic stress inhibits phagocytic activity of ARPE-19 cells; effect of donors' age and antioxidants.

    abstract::The risk of chronic oxidative stress in the retinal pigment epithelium (RPE) increases with age due to accumulation of the photoreactive age pigment lipofuscin (LFG). Here, we asked whether sublethal and weakly lethal photic stress, induced by irradiation of ARPE-19 cells containing phagocytised LFG, affected the cell...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Olchawa MM,Furso JA,Szewczyk GM,Sarna TJ

    更新日期:2017-10-01 00:00:00

  • Oxidative stress to rat lens in vitro: protection by taurine.

    abstract::The concentration of taurine is high in the lens. However, its function therein remains unknown. Studies from other tissues suggest that in addition to several other modes of action, it acts as an antioxidant. We therefore hypothesize that taurine may be a part of the antioxidant defense mechanisms involved in protect...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Devamanoharan PS,Ali AH,Varma SD

    更新日期:1998-09-01 00:00:00

  • EPR studies on the effects of complexation of heme by hemopexin upon its reactions with organic peroxides.

    abstract::Hemopexin, a heme-binding serum glycoprotein, is thought to play an important role in the prevention of oxidative damage that may be catalysed by free heme. Through the use of EPR techniques, the generation of free radicals from organic hydroperoxides by heme and heme-hemopexin complexes, and the concomitant formation...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Timmins GS,Davies MJ,Song DX,Muller-Eberhard U

    更新日期:1995-12-01 00:00:00

  • Linoleic acid hydroperoxide favours hypochlorite- and myeloperoxidase-induced lipid peroxidation.

    abstract::Liposomes composed of soybean phosphatidylcholine were peroxidized using the reagent sodium hypochlorite or the myeloperoxidase-hydrogen peroxide-Cl- system. Linoleic acid hydroperoxide previously prepared from linoleic acid by means of lipoxidase was incorporated into liposomes. The yield of thiobarbituric acid react...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Panasenko OM,Arnhold J

    更新日期:1999-06-01 00:00:00

  • Lymphocyte antioxidant response and H2O2 production after a swimming session: gender differences.

    abstract::This study evaluated the gender differences in response to intense exercise on lymphocyte hydrogen peroxide production, nitric oxide handling and mitochondrial superoxide dismutase (MnSOD) activity and gene expression. Fifteen males and nine females participated voluntarily in the study and performed a swimming sessio...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Sureda A,Ferrer MD,Tauler P,Tur JA,Pons A

    更新日期:2008-04-01 00:00:00

  • Scavenging of benzylperoxyl radicals by carotenoids.

    abstract::Carotenoids scavenge simple lipid-like alkylperoxyl radicals. However, the rate constant is too low to be determined directly and the mechanism is likewise not known with certainty [Mortensen, A. and Skibsted, L.H. (1998) FEBS Lett. 426, 392-396]. It is demonstrated that carotenoids react with peroxyl radicals only sl...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Mortensen A

    更新日期:2002-02-01 00:00:00

  • EPR and electrochemical quantification of oxygen using newly synthesized para-silylated triarylmethyl radicals.

    abstract::Novel silylated triarylmethyl (TAM) radicals based on TAM core CT-03 and their electron paramagnetic resonance (EPR) spectra are evaluated as a function of oxygen concentration. Combination of peak-to-peak linewidth of the EPR signal and electrochemical determination allows designing a method for oxygen quantification...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Boutier-Pischon A,Auger F,Noël JM,Almario A,Frapart YM

    更新日期:2015-03-01 00:00:00

  • Genes, ageing and longevity in humans: problems, advantages and perspectives.

    abstract::Many epidemiological data indicate the presence of a strong familial component of longevity that is largely determined by genetics, and a number of possible associations between longevity and allelic variants of genes have been described. A breakthrough strategy to get insight into the genetics of longevity is the stu...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Salvioli S,Olivieri F,Marchegiani F,Cardelli M,Santoro A,Bellavista E,Mishto M,Invidia L,Capri M,Valensin S,Sevini F,Cevenini E,Celani L,Lescai F,Gonos E,Caruso C,Paolisso G,De Benedictis G,Monti D,Franceschi C

    更新日期:2006-12-01 00:00:00

  • A novel method using 8-hydroperoxy-2'-deoxyguanosine formation for evaluating antioxidative potency.

    abstract::Degenerative diseases such as cancer are induced by oxidative genetic damage. Antioxidants can scavenge reactive oxygen species, but to prevent disease, they must do so quickly, before the DNA bases are damaged. In the present study, a novel method was established for evaluating the potency of antioxidants employing 2...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Sakakibara H,Ashida H,Kanazawa K

    更新日期:2002-03-01 00:00:00

  • Adaptation to multiday ozone exposure is associated with a sustained increase of bronchoalveolar uric acid.

    abstract::The phenomenon of ozone tolerance is described, but the underlying mechanisms remain unknown. We tested whether adaptation to multiday ozone exposure was related to an upregulated pulmonary antioxidant defence. Six calves were exposed to 0.75 ppm ozone, 12 h day(-1) for seven consecutive days. Pulmonary function tests...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Kirschvink N,Fiévez L,Bureau F,Degand G,Maghuin-Rogister G,Smith N,Art T,Lekeux P

    更新日期:2002-01-01 00:00:00

  • Pharmaco/ferrokinetic-related pro-oxidant activity of deferiprone in beta-thalassemia.

    abstract::The potential of free radical formation in serum of beta-thalassemia/Hb E patients receiving a single oral dose of 25 mg/kg body weight of deferiprone, a bidentate orally active iron chelator, was evaluated using EPR/spin trapping technique. In the presence of ascorbic acid and tert-butylhydroperoxide, EPR signals of ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Jirasomprasert T,Morales NP,Limenta LM,Sirijaroonwong S,Yamanont P,Wilairat P,Fucharoen S,Chantharaksri U

    更新日期:2009-05-01 00:00:00

  • Nitric oxide (•NO) generation but not ROS plays a major role in silibinin-induced autophagic and apoptotic death in human epidermoid carcinoma A431 cells.

    abstract::Silibinin, a major active constituent of silymarin, is clinically used as a hepatoprotectant, and in recent years, it has been used for the treatment of cancer in China. Because the mechanism of silibinin action on cancer cells was still unclear, we investigated the contribution of silibinin to the induction of apopto...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Yu Y,Fan SM,Yuan SJ,Tashiro S,Onodera S,Ikejima T

    更新日期:2012-11-01 00:00:00

  • Suppression of mitochondrial NADP(+)-dependent isocitrate dehydrogenase activity enhances curcumin-induced apoptosis in HCT116 cells.

    abstract::Curcumin is a polyphenol derived from the plant Curcuma longa that induces apoptotic cell death in malignant cancer cell lines. It has been shown previously that mitochondrial NADP(+)-dependent isocitrate dehydrogenase (IDPm) plays an essential role in defense against oxidative stress by supplying NADPH for antioxidan...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Jung KH,Park JW

    更新日期:2011-04-01 00:00:00

  • Excess processing of oxidative damaged bases causes hypersensitivity to oxidative stress and low dose rate irradiation.

    abstract::Ionizing radiations such as X-ray and γ-ray can directly or indirectly produce clustered or multiple damages in DNA. Previous studies have reported that overexpression of DNA glycosylases in Escherichia coli (E. coli) and human lymphoblast cells caused increased sensitivity to γ-ray and X-ray irradiation. However, the...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Yoshikawa Y,Yamasaki A,Takatori K,Suzuki M,Kobayashi J,Takao M,Zhang-Akiyama QM

    更新日期:2015-10-01 00:00:00

  • Interactions of malondialdehyde and 4-hydroxynonenal with rat liver plasma membranes and their effect on binding of prostaglandin E2 by specific receptors.

    abstract::We investigated effect of aldehydic products of lipid peroxidation, malondialdehyde (MDA) and 4-hydroxynonenal (HNE) on prostaglandin (PG) E2 receptors of liver plasma membranes. The modification of the membranes by MDA diminished PGE2 binding, decreasing receptor affinity for PGE2 and receptor density whereas HNE inc...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Buko VU,Artsukevich A,Zavodnik I,Maltsev A,Sushko L,Zimmermann T,Dianzani MU

    更新日期:1996-11-01 00:00:00

  • The identification of antioxidants in dark soy sauce.

    abstract::Soy sauce is a traditional fermented seasoning in Asian countries, that has high antioxidant activity in vitro and some antioxidant activity in vivo. We attempted to identify the major antioxidants present, using the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay as a guide. 3-Hydroxy-2-methyl-4H-p...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Wang H,Jenner AM,Lee CY,Shui G,Tang SY,Whiteman M,Wenk MR,Halliwell B

    更新日期:2007-04-01 00:00:00

  • Knockdown of LINC00473 promotes radiosensitivity of non-small cell lung cancer cells via sponging miR-513a-3p.

    abstract::Non-small cell lung cancer (NSCLC) is the most common form of lung cancer. Radioresistance is a significant obstacle in NSCLC radiotherapy. Long non-coding RNA LINC00473 has been found to impact the radiotherapy in several malignant tumours. This study aimed to investigate the underlying role and mechanism of LINC0047...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Qin P,Li Y,Liu J,Wang N

    更新日期:2020-10-01 00:00:00

  • Diosgenin inhibits superoxide generation in FMLP-activated mouse neutrophils via multiple pathways.

    abstract::Diosgenin possesses anti-inflammatory and anticancer properties. Activated neutrophils produce high concentrations of the superoxide anion which is involved in the pathophysiology of inflammation-related diseases and cancer. In the present study, the inhibitory effect and possible mechanisms of diosgenin on superoxide...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Lin Y,Jia R,Liu Y,Gao Y,Zeng X,Kou J,Yu B

    更新日期:2014-12-01 00:00:00

  • Severe total hepatic ischemia and reperfusion: relationship between very high alpha-tocopherol uptake and lipid peroxidation.

    abstract::Reperfusion injury of the liver occurs in liver transplantation and in major hepatectomies. It triggers a severe oxidative stress that leads to increased lipid peroxidation. In our study we examined the effect of parenteral supranutritional administration of alpha-tocopherol, a vitamin that plays a key role in the end...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Giakoustidis D,Kontos N,Iliadis S,Papageorgiou G,Tsantilas D,Spyridis C,Papazoglou K,Botsoglou N,Dimitriadou A,Giakoustidis E

    更新日期:2001-08-01 00:00:00

  • Curcumin prevents maleate-induced nephrotoxicity: relation to hemodynamic alterations, oxidative stress, mitochondrial oxygen consumption and activity of respiratory complex I.

    abstract::The potential protective effect of the dietary antioxidant curcumin (120 mg/Kg/day for 6 days) against the renal injury induced by maleate was evaluated. Tubular proteinuria and oxidative stress were induced by a single injection of maleate (400 mg/kg) in rats. Maleate-induced renal injury included increase in renal v...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Tapia E,Sánchez-Lozada LG,García-Niño WR,García E,Cerecedo A,García-Arroyo FE,Osorio H,Arellano A,Cristóbal-García M,Loredo ML,Molina-Jijón E,Hernández-Damián J,Negrette-Guzmán M,Zazueta C,Huerta-Yepez S,Reyes JL,Madero M

    更新日期:2014-11-01 00:00:00

  • Pharmacology, genomics, and the evolutionary biology of ageing.

    abstract::Aging is a multifold process affected by many genes and thus many biochemical pathways. This conclusion is underscored by the failure to find simple central controls for the aging process during the 20th Century. This situation poses a fundamental challenge to anti-aging medicine: how to develop effective therapies fo...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Rose MR,Mueller LD,Long AD

    更新日期:2002-12-01 00:00:00

  • Antioxidants inhibit the human cortical neuron apoptosis induced by hydrogen peroxide, tumor necrosis factor alpha, dopamine and beta-amyloid peptide 1-42.

    abstract::Several substances related to the neurodegenerative diseases of Alzheimer and Parkinson, such as hydrogen peroxide, tumor necrosis factor alpha, dopamine and beta-amyloid peptide 1-42, have been shown to induce apoptosis in tumoral cell lines and rat neurons but not in human neurons. Moreover, the role of mitochondria...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Medina S,Martínez M,Hernanz A

    更新日期:2002-11-01 00:00:00

  • Toxicity of polyunsaturated fatty acid esters for human monocyte-macrophages: the anomalous behaviour of cholesteryl linolenate.

    abstract::We have investigated the toxicity to human monocytemacrophages, and susceptibility to oxidation, of different individual dietary fatty acids in cholesterol esters and triglycerides, added to the cell cultures as coacervates with bovine serum albumin. Toxicity was assessed using release of radioactivity from cells prel...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Hardwick SJ,Carpenter KL,Law NS,Van Der Veen C,Marchant CE,Hird R,Mitchinson MJ

    更新日期:1997-04-01 00:00:00