Abstract:
:Validation of markers requires two different steps. The first one is the analytical validation including analytical procedures, definition of standard reference, and round-tests analysis. The second one is the assessment of the biological relevance of the selected marker. This biological relevance may be in the field of physiology to measure a physiological function, or in the area of pathology to measure a disease. Validation of markers is a time-consuming task that needs cooperation between laboratories. On the other hand a validated marker is a keystone for scientists to share their results and to explore more efficiently a given biological system.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Antoine JM,Diplock ATkeywords:
subject
Has Abstractpub_date
2000-11-01 00:00:00pages
S117-20eissn
1071-5762issn
1029-2470journal_volume
33 Supplpub_type
杂志文章,评审abstract::Approaching living systems, aqueous solutions are appropriate to characterize antioxidants, whereas the frequently used standard 1,1-diphenyl-2-picrylhydrazyl (DPPH) is insoluble in water. Therefore, mixed water-ethanol solvents were investigated using the electron paramagnetic resonance (EPR) spectroscopy. Two forms ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600930014
更新日期:2007-04-01 00:00:00
abstract::8-Oxoguanine (8-oxoG) is the most common DNA base modification in the mammalian genome, associated with oxidative stress. Here we analysed the alterations in the distribution of 8-oxoG across the entire murine genome, before and after an elevation of oxidative stress by the use of ferric nitrilotriacetate (Fe-NTA) as ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2020.1733548
更新日期:2020-04-16 00:00:00
abstract::Oxidative stress is associated with a range of inflammatory lung diseases including asthma, adult respiratory distress syndrome, idiopathic pulmonary fibrosis, pneumonia, lung transplantation, chronic obstructive pulmonary disease, cystic fibrosis, bronchiectasis and lung cancer. Increased concentrations of reactive o...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760310001623331
更新日期:2003-12-01 00:00:00
abstract::The hydroxyl radical (.OH)-scavenging activity of d-2-[4-(3-methyl-2-thienyl)phenyl]propionic acid (M-5011), a novel nonsteroidal anti-inflammatory drug (NSAID), and that of several other NSAIDs were investigated by the hyaluronic acid (HA) degradation method and the electron spin resonance (ESR) spin-trapping techniq...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709065781
更新日期:1997-10-01 00:00:00
abstract:INTRODUCTION:Nuclear factor, erythroid 2 like 2 (NFE2L2) is an important transcription factor that protects cells from oxidative stress (OS). NFE2L2 deficiency in placentas is associated with pregnancy complications. We have demonstrated that elevated OS existed in placental shares of the smaller fetus in selective int...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1315113
更新日期:2017-04-01 00:00:00
abstract::Dietary flavonoids, present in different amount in foods, are associated with the prevention of hypertension, but little is known about the interactions between them. The aim of this study was to explore the effect of quercetin (Q), catechin (C) and the mixture, on Angiotensin II (AngII)-induced redox-dependent signal...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2012.660527
更新日期:2012-05-01 00:00:00
abstract::The ability of a range of dietary flavonoids to inhibit low-density lipoprotein (LDL) oxidation in vitro was tested using a number of different methods to assess oxidative damage to LDL. Overall quercetin was the most effective inhibitor of oxidative damage to LDL in vitro. On this basis, a diet enriched with onions a...
journal_title:Free radical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1080/10715760000300951
更新日期:2000-10-01 00:00:00
abstract::Silibinin, a major active constituent of silymarin, is clinically used as a hepatoprotectant, and in recent years, it has been used for the treatment of cancer in China. Because the mechanism of silibinin action on cancer cells was still unclear, we investigated the contribution of silibinin to the induction of apopto...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2012.715369
更新日期:2012-11-01 00:00:00
abstract::Oxidative stress is a major pathogenetic event occurring in several liver disorders and is a major cause of liver damage due to Ischemia/Reperfusion (I/R) during liver transplantation. While several markers of chronic oxidative stress are well known, early protein targets of oxidative injury are not well defined. In o...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760400029603
更新日期:2005-03-01 00:00:00
abstract::It is well known that antioxidants such as AA (reduced ascorbate), glutathione (GSH) (reduced glutathione) and melatonin can delay seed ageing. Can they recover aged seed? Artificial aged maize seeds were obtained and their reduced germination rate (GR) and high lipid peroxidation were recorded. Exogenous melatonin wa...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1375099
更新日期:2017-10-01 00:00:00
abstract::The effect of antioxidants on ammonia induced CNS and renal pathobiology in 10 sheep infested by L. Cuprina larvae was investigated. The condition produces severe dermatitis, proliferation of macrophages and hyperammonaemia, and free radicals may therefore be involved in the pathogenesis. Five of the sheep (treated gr...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769409056553
更新日期:1994-07-01 00:00:00
abstract::MCI-186 (3-methyl-1-phenyl-2-pyrazolin-5-one) is a newly developed antioxidant which has been shown to reduce brain edema in cerebral ischemia through inhibition of the lipoxygenase pathway of arachidonic acid. However, its effect on myocardial reperfusion injury after prolonged ischemia has not yet been demonstrated....
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769609088034
更新日期:1996-05-01 00:00:00
abstract::Identification of free radicals was performed for the reaction mixtures of autoxidized 1,2-dilinoleoylphosphatidylcholine (DLPC) with ferrous ions (or DLPC hydroperoxide with ferrous ions) and of DLPC with soybean lipoxygenase using electron spin resonance (ESR), high performance liquid chromatography (HPLC)--ESR and ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500232073
更新日期:2005-09-01 00:00:00
abstract::Orellanine is the tetrahydroxylated and di-N-oxidized bipyridine toxin extracted from several Cortinarius mushrooms among them C. orellanus. The pathogenic mechanism involved in the C. orellanus-poisoning by orellanine leading to kidney impairment is not yet fully understood until now. Electron spin resonance (ESR) sp...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769809066887
更新日期:1998-05-01 00:00:00
abstract::In a prospective, double-blind, randomised placebo-controlled study, we tested the hypothesis that a new formulation consisting of wheat gliadin chemically combined with a vegetal (thus orally effective) preparation of superoxide dismutase (SOD) allows to prevent hyperbaric oxygen (HBO)-induced oxidative cell stress. ...
journal_title:Free radical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1080/10715760412331273197
更新日期:2004-09-01 00:00:00
abstract::Along with differentiation of mouse stromal preadipocytes OP9 into adipocytes, intracellular ROS, especially superoxide anion radicals detected by NBT reduction assay, were found to appreciably increase, mainly in cytoplasmic area, parallelling with increases in intracellular lipid-droplet accumulation, whereas undiff...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2010.499905
更新日期:2010-09-01 00:00:00
abstract::Glycation of proteins or other biomolecules and their further long-term degradation result in the formation of advanced glycation end products, AGEs. AGEs and other ligands interact with their receptors, RAGEs, localized to a variety of tissues, but mainly in endothelium and vascular wall cells. This interaction trigg...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2013.815347
更新日期:2013-08-01 00:00:00
abstract::The present study was designed to determine whether hydroxymethylglutaryl-CoA reductase inhibitors (statins) modulate the NO production via iNOS in adipocytes stimulated by lipopolysaccharide (L) and tumour necrosis factor-alpha (T). Well-differentiated 3T3-L1 adipocytes significantly produced NO by LT-treatment. Pre-...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701534368
更新日期:2007-09-01 00:00:00
abstract::To study the role of oxidative stress in hypertension and pre-hypertension, this study analysed plasma levels of reactive carbonyl species (RCS) in 1204 Chinese Han adults. Results showed a statistically significant positive correlation (p < 0.001) between blood pressure and plasma RCS levels with or without being adj...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2011.557723
更新日期:2011-05-01 00:00:00
abstract::The COST Action CM1201 "Biomimetic Radical Chemistry" has been active since December 2012 for 4 years, developing research topics organized into four working groups: WG1 - Radical Enzymes, WG2 - Models of DNA damage and consequences, WG3 - Membrane stress, signalling and defenses, and WG4 - Bio-inspired synthetic stra...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715762.2016.1248961
更新日期:2016-11-01 00:00:00
abstract::Alzheimer's disease (AD) is characterised as a provoked inflammatory response and oxidative stress along with amyloid β peptide (Aβ) deposition and neurofibrillary tangles in the brain, and effective treatment is greatly needed. Molecular hydrogen, which has been proposed to be an antioxidant that selectively reduces ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2018.1460749
更新日期:2018-12-01 00:00:00
abstract::A glycomic approach to the identification of target molecules in glycosyltransferase gene targeting mice is a promising strategy to understand the biological significance of glycosyltransferase genes in vivo. In order to understand the biological effects of N-acetylglucosaminyltransferase III (GnT-III) on tumor format...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576021000005258
更新日期:2002-08-01 00:00:00
abstract::Sublytic levels (microM) of hemin destabilized RBC membrane as indicated by ghost fragmentation pattern using a laser viscodiffractometer. Furthermore, electron microscopic study shows that 5 microM of hemin induced echinocytic transformation whereas higher hemin concentration (40 microM) induced spherocytic transform...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709097838
更新日期:1997-07-01 00:00:00
abstract::The review deals with impairment of Ca(2+)-ATPases by high glucose or its derivatives in vitro, as well as in human diabetes and experimental animal models. Acute increases in glucose level strongly correlate with oxidative stress. Dysfunction of Ca(2+)-ATPases in diabetic and in some cases even in nondiabetic conditi...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2013.807923
更新日期:2013-08-01 00:00:00
abstract::There is a correlation between oxidative stress generated by diethylnitrosamine (DEN) metabolism and liver cancer development. Quercetin is a flavonoid with anti-carcinogenic and antioxidant properties. This study demonstrates the mechanism of action for the chemopreventive effect of quercetin. A 10 mg/kg dose of quer...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760802626535
更新日期:2009-02-01 00:00:00
abstract::Coenzyme Q10 (CoQ10) is an essential cofactor of the electron transport gene as well as an important antioxidant, which is particularly effective within mitochondria. A number of prior studies have shown that it can exert efficacy in treating patients with known mitochondrial disorders. We investigated the potential u...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760290021315
更新日期:2002-04-01 00:00:00
abstract::The risk of chronic oxidative stress in the retinal pigment epithelium (RPE) increases with age due to accumulation of the photoreactive age pigment lipofuscin (LFG). Here, we asked whether sublethal and weakly lethal photic stress, induced by irradiation of ARPE-19 cells containing phagocytised LFG, affected the cell...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1380307
更新日期:2017-10-01 00:00:00
abstract::Effects of 12 wk exercise training on oxidative stress were examined in elderly humans. We measured oxidative stress during a 45 min cycling test by using antipyrine hydroxylation products. Antipyrine breakdown is independent of blood flow to the liver, which is important during exercise. Furthermore, antipyrine react...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100300951
更新日期:2001-10-01 00:00:00
abstract::Ceruloplasmin (CP) is a blue copper glycoprotein with multiple physiological functions including ferroxidase and oxidase activities. CP is also an important serum oxygen free radical (OFR) scavenger and antioxidant, exerting cardioprotective and antifibrillatory actions. Although it has been reported that CP activitie...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760310001624079
更新日期:2004-01-01 00:00:00
abstract::Oxidized low density lipoprotein (LDL) plays an important role in atherogenesis. It is generally thought that LDL is mainly oxidized in the intima of vessel walls, surrounded by hydrophilic antioxidants and proteins such as albumin. The aim of this study was to investigate the possible interrelationships between oxida...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760290006466
更新日期:2002-02-01 00:00:00