Abstract:
:A small part of cellular iron, usually called 'labile iron pool' (LIP), is not securely stored and has the potential to catalyse the formation of highly reactive oxygen species. The present work estimated LIP levels in human white cells by using the analytical power of flow cytometry. The method relies essentially on already established principles but has the added value of monitoring LIP in different subpopulations of human blood cells concurrently in a single sample. Examination of 41 apparently healthy individuals revealed a positive correlation between LIP levels and the age of the donors (r=0.656, 0.572 and 0.702 for granulocytes, lymphocytes and monocytes, respectively, p<0.0001), indicating that cells of older individuals are prone to oxidations in conditions of oxidative stress. It is suggested that LIP estimation may represent a valuable tool in examinations searching for links between iron and a variety of oxidative stress-related pathological conditions.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Doulias PT,Vlachou C,Boudouri C,Kanavaros P,Siamopoulos KC,Galaris Ddoi
10.1080/10715760801911649subject
Has Abstractpub_date
2008-03-01 00:00:00pages
253-9issue
3eissn
1071-5762issn
1029-2470pii
791484422journal_volume
42pub_type
杂志文章abstract::N-nitroso species have recently been detected in animal tissues. Protein N-nitrosotryptophan is the best candidate for this N-nitroso pool. N-nitrosation of N-blocked trytophan derivatives like melatonin (MelH) by N2O3 or peroxynitrite (ONOOH/ONOO- ) has been observed under conditions of pH and reagent concentrations ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600693414
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abstract::The use of antioxidants to prevent intracellular free radical damage is an area currently attracting considerable research interest. The compound 2',7'-dichlorofluorescin diacetate (DCFH-DA) is a probe for intracellular peroxide formation commonly used in such studies. During our studies we unexpectedly found that inc...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709097782
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abstract::In severe COPD patients, oxidative stress, which is involved in their peripheral muscle dysfunction, increases in response to exercise. In this study, muscle oxidative stress was explored after quadriceps magnetic stimulation training. A randomized controlled study was conducted on very severe COPD patients, who under...
journal_title:Free radical research
pub_type: 杂志文章,随机对照试验
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更新日期:2008-11-01 00:00:00
abstract::Rabbits were fed a diet containing 1% cholesterol for 8 weeks and the levels of iron and oxidized lipids in liver analysed using atomic absorption spectroscopy and gas chromatography-mass spectrometry. A non-significant trend to an increase in iron level, but significant increases in the lipid peroxidation products, F...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760802691455
更新日期:2009-03-01 00:00:00
abstract::We have determined the effects of chronic vitamin C intake on neutrophil and lymphocyte antioxidant defences during the acute phase immune response induced by intense exercise. Blood samples were taken from 16 voluntary athletes in basal conditions, both immediately after and 1 h after a duathlon competition. Sportsme...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576031000150454
更新日期:2003-09-01 00:00:00
abstract::Oxidation of low density lipoprotein (LDL) has been implicated in the pathogenesis of atherosclerosis. The most common technique for measuring the oxidation of lipoproteins is the continuous measurement of the formation of conjugated diene at OD 234 nm. The concept of "lag time", derived from such measurements, has be...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715769809065814
更新日期:1998-06-01 00:00:00
abstract::The present study was designed to determine whether hydroxymethylglutaryl-CoA reductase inhibitors (statins) modulate the NO production via iNOS in adipocytes stimulated by lipopolysaccharide (L) and tumour necrosis factor-alpha (T). Well-differentiated 3T3-L1 adipocytes significantly produced NO by LT-treatment. Pre-...
journal_title:Free radical research
pub_type: 杂志文章
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abstract::The hydroxyl radical adducts of 5,5 dimethyl-1-pyrolline-N-oxide (DMPO) and 3,3,5,5 tetramethyl-1-pyrolline-N-oxide (TMPO) formed in the presence of hydrogen peroxide and FeII are normally quite stable, but in the presence of 5-20 micromolar myoglobin their ESR signals decay rapidly. This decay probably reflects furth...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769409145632
更新日期:1994-05-01 00:00:00
abstract::Tropolones, the naturally occurring compounds responsible for the durability of heartwood of several cupressaceous trees, have been shown to possess both metal chelating and antioxidant properties. However, little is known about the ability of tropolone and its derivatives to protect cultured cells from oxidative stre...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760400017244
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abstract::The significance of 5-lipoxygenase and myeloperoxidase activities has not been extensively studied among young male smokers. Leukotriene B(4), 20-hydroxy-leukotriene B(4), 20-carboxy-leukotriene B(4) and 3-chlorotyrosine were measured in plasma and urinary samples of young male smokers at 8 hours following cigarette a...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2012.701291
更新日期:2012-10-01 00:00:00
abstract::The increasing knowledge on the participation of free radicals in many diverse clinical and pathological conditions, has consequently expanded the search for new and versatile antioxidants aimed at combating oxidative stress. Our interest in this field concerns aromatic indolinonic aminoxyls (nitroxides) which efficie...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769900301621
更新日期:1999-08-01 00:00:00
abstract::The in vitro toxicity of the reactive oxygen species generating enzyme xanthine oxidoreductase (XOR) to human peripheral blood lymphocytes was studied after stimulation with phytohaemoagglutinin or anti-CD3/CD28 antibodies. Apoptosis and necrosis were induced by the XOR/hypoxanthine system in a time- and concentration...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100301191
更新日期:2001-12-01 00:00:00
abstract::Paraquat is a synthetic bipyridylium salt widely used as herbicide and defoliant. Enzyme-catalyzed redoxcycling of paraquat generates oxygen radicals. The toxic, even lethal, effects of paraquat are due to free radical-mediated tissue injury. Ocular lesions, sometimes quite severe, have been observed following acciden...
journal_title:Free radical research
pub_type: 杂志文章
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更新日期:1995-07-01 00:00:00
abstract::Tumour necrosis factor alpha (TNF alpha) cytotoxicity is mediated, at least in part, by oxidative stress and phospholipase A2 activation. The first post-receptor events to be observed in TNF alpha-sensitive lines are the generation of superoxide anion (O2-) within the mitochondria and the activation of phospholipase A...
journal_title:Free radical research
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doi:10.3109/10715769609149917
更新日期:1996-08-01 00:00:00
abstract::Lipid peroxidation (LPO) product accumulation in human tissues is a major cause of tissular and cellular dysfunction that plays a major role in ageing and most age-related and oxidative stress-related diseases. The current evidence for the implication of LPO in pathological processes is discussed in this review. New d...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2010.498478
更新日期:2010-10-01 00:00:00
abstract::Asthma is an allergic inflammation driven by the Th2 immune response with release of cytokines such as IL-4 and IL-13, which contribute to the airflow limitations and airway hyperresponsiveness (AHR). The involvement of oxidative stress in this process is well-established, but the specific role of the superoxide anion...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1364377
更新日期:2017-07-01 00:00:00
abstract::The widely used anti-cancer drug cisplatin imparts various toxic manifestations in the host, with nephrotoxicity being the most severe one. The trace element selenium shows antioxidant activity in both human and animals. The present study was designed to assess the chemoprotecting and chemoenhancing efficacy of a naph...
journal_title:Free radical research
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doi:10.3109/10715762.2015.1012079
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abstract::The antioxidant properties of protoporphyrin IX and related tetrapyrroles are poorly characterized. Therefore, eight tetrapyrroles, five of which are produced in vivo, were tested to assess their antioxidant capacities in the Salmonella reverse mutation, TEAC, FRAP and ORAC assays. Tertiary-butyl hydroperoxide (tert-B...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2012.715371
更新日期:2012-11-01 00:00:00
abstract::Intestinal injury is inevitable during exposure to high radiation doses and is a common side effect observed during abdominal/pelvic radiotherapy. Yet, no radiation countermeasures are available for gastrointestine (GI) injury management. The aim of this study is to determine the effects of podophyllotoxin and rutin i...
journal_title:Free radical research
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abstract::Recent research about the role of free radical derivatives of oxygen and nitrogen in biological systems has highlighted the possibility that antioxidants, such as vitamin E, that prevent these processes in vitro may be capable of carrying out a similar function in living organisms in vivo. There is increasing evidence...
journal_title:Free radical research
pub_type: 杂志文章,评审
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更新日期:1997-06-01 00:00:00
abstract::The aim of this study was to investigate the effect of HDL oxidation on PON1 paraoxonase activity. Also, we were interested in investigating the mechanism by which PON1 could be inactivated and the correlation between its enzymatic activity and the antioxidant properties of HDL. Three different oxidation systems were ...
journal_title:Free radical research
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journal_title:Free radical research
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doi:10.1080/1071576021000028316
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abstract::Myeloperoxidase (MPO), a major constituent of neutrophils, catalyzes the production of hypochlorous acid (HOCl) from hydrogen peroxide (H2O2) and chloride anion. We have previously reported that MPO-deficient (MPO(-/-)) neutrophils produce greater amount of macrophage inflammatory protein-2 (MIP-2) in vitro than do wi...
journal_title:Free radical research
pub_type: 杂志文章
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更新日期:2013-05-01 00:00:00
abstract::The effect of antioxidants on ammonia induced CNS and renal pathobiology in 10 sheep infested by L. Cuprina larvae was investigated. The condition produces severe dermatitis, proliferation of macrophages and hyperammonaemia, and free radicals may therefore be involved in the pathogenesis. Five of the sheep (treated gr...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769409056553
更新日期:1994-07-01 00:00:00
abstract::Tetrahydrobiopterin (BH4) is a ubiquitous pteridine metabolite that serves as a NOS cofactor. Recently, we showed that BH4 efficiently inhibits superoxide generation from the heme group at the oxygenase domain of eNOS. This role indicates that BH4 acts as a redox switch in the catalytic mechanism of the enzyme, which ...
journal_title:Free radical research
pub_type: 杂志文章,评审
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更新日期:2003-02-01 00:00:00
abstract::We investigated hepatoprotective activity and antioxidant effect of the 2,5-dihydroxy-4,3'-di(beta-D-glucopyranosyloxy)-trans-stilbene that purified from Morus bombycis Koidzumi roots against CCl4-induced liver damage in rats. The 2,5-dihydroxy-4,3'-di(beta-D-glucopyranosyloxy)-trans-stilbene displayed dose-dependent ...
journal_title:Free radical research
pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
abstract::Gamma-glutamyltransferase (GGT) plays a central role in the homeostasis of the antioxidant glutathione (GSH). The expression of GGT has been shown to be upregulated after oxidative stress, but the signalling pathways implicated remain poorly characterized. The results here show that acute exposure of CC531 cells to ox...
journal_title:Free radical research
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更新日期:2007-12-01 00:00:00
abstract::Coenzyme Q10 (CoQ10) is an essential cofactor of the electron transport gene as well as an important antioxidant, which is particularly effective within mitochondria. A number of prior studies have shown that it can exert efficacy in treating patients with known mitochondrial disorders. We investigated the potential u...
journal_title:Free radical research
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更新日期:2002-04-01 00:00:00
abstract::The phenomenon of ozone tolerance is described, but the underlying mechanisms remain unknown. We tested whether adaptation to multiday ozone exposure was related to an upregulated pulmonary antioxidant defence. Six calves were exposed to 0.75 ppm ozone, 12 h day(-1) for seven consecutive days. Pulmonary function tests...
journal_title:Free radical research
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abstract::Oxidatively induced damage caused by free radicals and other DNA-damaging agents generate a plethora of products in the DNA of living organisms. There is mounting evidence for the involvement of this type of damage in the etiology of numerous diseases including carcinogenesis. For a thorough understanding of the mecha...
journal_title:Free radical research
pub_type: 杂志文章,评审
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更新日期:2015-05-01 00:00:00