Flow cytometric estimation of 'labile iron pool' in human white blood cells reveals a positive association with ageing.

Abstract:

:A small part of cellular iron, usually called 'labile iron pool' (LIP), is not securely stored and has the potential to catalyse the formation of highly reactive oxygen species. The present work estimated LIP levels in human white cells by using the analytical power of flow cytometry. The method relies essentially on already established principles but has the added value of monitoring LIP in different subpopulations of human blood cells concurrently in a single sample. Examination of 41 apparently healthy individuals revealed a positive correlation between LIP levels and the age of the donors (r=0.656, 0.572 and 0.702 for granulocytes, lymphocytes and monocytes, respectively, p<0.0001), indicating that cells of older individuals are prone to oxidations in conditions of oxidative stress. It is suggested that LIP estimation may represent a valuable tool in examinations searching for links between iron and a variety of oxidative stress-related pathological conditions.

journal_name

Free Radic Res

journal_title

Free radical research

authors

Doulias PT,Vlachou C,Boudouri C,Kanavaros P,Siamopoulos KC,Galaris D

doi

10.1080/10715760801911649

subject

Has Abstract

pub_date

2008-03-01 00:00:00

pages

253-9

issue

3

eissn

1071-5762

issn

1029-2470

pii

791484422

journal_volume

42

pub_type

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