Abstract:
:Epidemiology studies have shown that consumption of fruits and vegetables is associated with the prevention of chronic diseases such as cancer and cardiovascular disease. Induction of cellular phase II detoxifying enzymes is associated with cancer preventive potential. Phenolsulfotransferases (PSTs) are traditionally known as phase II drug-metabolizing or detoxifying enzymes that facilitate the removal of drugs and other xenobiotic compounds. Phenolic acids are known to increase the activities of PSTs. In the present study, human HepG2 cells were used as model to investigate the influence of twenty vegetables on human PST activity and to evaluate the relationships to their antioxidant activity and total phenolics content. The result showed that PST-P activity was significantly (p < 0.01) induced by asparagus, broccoli, cauliflower, celery and eggplant, whereas PST-M activity was induced by asparagus, broccoli, carrot, eggplant and potato at a concentration of 100 microg/ml. The vegetable extracts that induced both forms of PSTs activities were found to have higher antioxidant capacities and total phenolic content in the oxygen radical absorbance capacity (ORAC) and Folin-Ciocalteu assay. The major polyphenols in broccoli, the most potential inducer in both forms of PSTs activities, was antioxidant phenolic acids. HPLC retention times and standard spiked indicated the presence of gallic acid, p-hydroxybenzoic acid, p-coumaric acid, gentisic acid and ferulic acid in broccoli. The overall effect of vegetables tested on the activity of PST-P was well correlated to their ORAC value and total phenolics content (r= 0.82, p < 0.05 and r = 0.78, p < 0.05). These results imply that vegetables have a capability of inducing PST activity, and the PST induction may be possibly ascribed to antioxidant phenolic acids in vegetable extracts.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Yeh CT,Yen GCdoi
10.1080/10715760500150424keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
893-904issue
8eissn
1071-5762issn
1029-2470pii
J7H8LH6044804Q05journal_volume
39pub_type
杂志文章abstract::Formation of the alpha-hydroxyethyl radical (CH3 degree CHOH) has already been extensively demonstrated after ethanol metabolism in the liver. Despite favourable conditions, this formation in the brain has remained speculative since there is no direct experimental evidence in intact brain cells. In this preliminary st...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709097868
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600946432
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journal_title:Free radical research
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journal_title:Free radical research
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journal_title:Free radical research
pub_type: 杂志文章,评审
doi:
更新日期:2000-11-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
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journal_title:Free radical research
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pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1999-04-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2010.532495
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doi:10.1080/10715762.2020.1733548
更新日期:2020-04-16 00:00:00
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journal_title:Free radical research
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1133906
更新日期:2016-01-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2015.1036052
更新日期:2015-01-01 00:00:00
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更新日期:2009-02-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2010.536838
更新日期:2011-04-01 00:00:00
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doi:10.3109/10715769609149069
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pub_type: 杂志文章,评审
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journal_title:Free radical research
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journal_title:Free radical research
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pub_type: 临床试验,杂志文章
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abstract::Melatonin is an endogenous indolamine, classically known as a light/dark regulator. Besides classical functions, melatonin has also showed to have a wide range of antitumoral effects in numerous cancer experimental models. However, no definite mechanism has been described to explain the whole range of antineoplasic ef...
journal_title:Free radical research
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