Abstract:
:Formation of the alpha-hydroxyethyl radical (CH3 degree CHOH) has already been extensively demonstrated after ethanol metabolism in the liver. Despite favourable conditions, this formation in the brain has remained speculative since there is no direct experimental evidence in intact brain cells. In this preliminary study, the formation of such a radical was observed after exposure of astrocytes and astrocytic C6 glioma cells to ethanol. These cells were studied because astrocyte integrity is essential for normal growth and functioning of neurons. The free radicals were detected by EPR spectroscopy using the spin trapping technique. Astrocytes appeared to be more sensitive than the C6 cells to free radical formation as the intensity of the signal was higher after exposure of the astrocytes and increased with time, a fact not observed after exposure of the C6 cells.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Gonthier B,Eysseric H,Soubeyran A,Daveloose D,Saxod R,Barret Ldoi
10.3109/10715769709097868subject
Has Abstractpub_date
1997-12-01 00:00:00pages
645-56issue
6eissn
1071-5762issn
1029-2470journal_volume
27pub_type
杂志文章abstract::Nitronyl nitroxides (NNs) are the paramagnetic probes that are capable of scavenging physiologically relevant reactive oxygen (ROS) and nitrogen (RNS) species, namely superoxide, nitric oxide (NO), and nitroxyl (HNO). NNs are increasingly considered as potent antioxidants and potential therapeutic agents. Understandin...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1013951
更新日期:2015-01-01 00:00:00
abstract::Aging is a multifold process affected by many genes and thus many biochemical pathways. This conclusion is underscored by the failure to find simple central controls for the aging process during the 20th Century. This situation poses a fundamental challenge to anti-aging medicine: how to develop effective therapies fo...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/1071576021000016535
更新日期:2002-12-01 00:00:00
abstract::The intracellular superoxide and glutathione disulphide concentrations increased in Penicillium chrysogeum treated with 50, 250 or 500 microM menadione (MQ). A significant increase in the intracellular peroxide concentration was also observed when mycelia were exposed to 250 or 500 microM MQ. The specific activity of ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769900300131
更新日期:1999-02-01 00:00:00
abstract::The significance of 5-lipoxygenase and myeloperoxidase activities has not been extensively studied among young male smokers. Leukotriene B(4), 20-hydroxy-leukotriene B(4), 20-carboxy-leukotriene B(4) and 3-chlorotyrosine were measured in plasma and urinary samples of young male smokers at 8 hours following cigarette a...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2012.701291
更新日期:2012-10-01 00:00:00
abstract::Assessment of oxidative stress status (OSS) in human tissues is still troublesome. Using an innovative EPR-radical-probe we successfully measured the instantaneous concentration of ROS directly in peripheral blood of athletes and normally active workers during 60 min controlled exercise. The probe employed was bis(1-h...
journal_title:Free radical research
pub_type: 临床试验,杂志文章
doi:10.1080/1071576031000076240
更新日期:2003-05-01 00:00:00
abstract::Prostaglandin E2 (PGE2) has been reported to play critical roles in cell fate decision by interacting with four types of prostanoid receptors such as EP1, EP2, EP3 and EP4. The present study was aimed at investigating the effect of the EP4-specific agonist CAY10598 in human colon cancer HCT116 cells. Our study reveale...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2014.951838
更新日期:2014-11-01 00:00:00
abstract::Asthma is an allergic inflammation driven by the Th2 immune response with release of cytokines such as IL-4 and IL-13, which contribute to the airflow limitations and airway hyperresponsiveness (AHR). The involvement of oxidative stress in this process is well-established, but the specific role of the superoxide anion...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1364377
更新日期:2017-07-01 00:00:00
abstract::Oxidative stress may initiate lipid peroxidation that generates ethane. Ethane, at low concentrations, is eliminated by pulmonary exhalation. Previous methods have not allowed frequent sampling, thus ethane kinetics has not been studied in man. A validated method over the range 3.8-100,000 ppb with a limit of quantita...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576031000107353
更新日期:2003-08-01 00:00:00
abstract::The effects of inorganic phosphate (Pi), the main intracellular membrane permeable anion capable of altering mitochondrial pH gradients (Delta pH), were measured on mitochondrial H2O2 release. As expected, Pi decreased Delta pH and increased the electric membrane potential (Delta Psi). Mitochondrial H2O2 release was s...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760400009258
更新日期:2004-10-01 00:00:00
abstract::The radiation-induced reactions of a water-soluble coumarin derivative, coumarin-3-carboxyl acid (C3CA), have been investigated in aqueous solutions by pulse radiolysis with a 35 MeV electron beam, final product analysis following (60)Co γ-irradiations and deterministic model simulations. Pulse radiolysis revealed tha...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2012.684879
更新日期:2012-07-01 00:00:00
abstract::N-nitroso species have recently been detected in animal tissues. Protein N-nitrosotryptophan is the best candidate for this N-nitroso pool. N-nitrosation of N-blocked trytophan derivatives like melatonin (MelH) by N2O3 or peroxynitrite (ONOOH/ONOO- ) has been observed under conditions of pH and reagent concentrations ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600693414
更新日期:2006-09-01 00:00:00
abstract::The oxidative stress possibly resulting from an inherited respiratory chain (RC) deficiency was investigated in a series of human cultured skin fibroblasts presenting either ubiquinone depletion or isolated defect of the various RC complexes. Taken as an index for superoxide overproduction, a significant induction of ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760290021216
更新日期:2002-04-01 00:00:00
abstract::Araucaria angustifolia (Bert.) O. Kuntze is a species critically endangered of extinction and its development and propagation is strongly affected by abiotic stress. We have previously shown the activation of uncoupling protein in A. angustifolia embryogenic stem cells subjected to cold stress. Now, we have furthered ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2018.1548767
更新日期:2019-01-01 00:00:00
abstract::Global gene expression profiles of livers from mice, fed diets differing in alpha-tocopherol content, were compared using DNA microarray technology. Three hundred and eighty nine genes were found to significantly differ in their expression level by a factor of 2 or higher between the high and the low alpha-tocopherol ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701416988
更新日期:2007-08-01 00:00:00
abstract::Oxidative stress is associated with a range of inflammatory lung diseases including asthma, adult respiratory distress syndrome, idiopathic pulmonary fibrosis, pneumonia, lung transplantation, chronic obstructive pulmonary disease, cystic fibrosis, bronchiectasis and lung cancer. Increased concentrations of reactive o...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760310001623331
更新日期:2003-12-01 00:00:00
abstract::Resorcinols (pentadecylresorcinol, olivetol, orcinol and resorcinol) exhibit antioxidant properties in liposomal systems. Antioxidant potency depends on the length of the alkyl chain. Pentadecylresorcinol has been demonstrated to be the most active antioxidant, indicating significance of its alkyl chain in a lipid bil...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769809070804
更新日期:1998-04-01 00:00:00
abstract::Over the last decades, oxidative stress has been described as a deleterious phenomenon contributing to numerous noncommunicable diseases such as cardiovascular disease, diabetes, and cancers. As many authors ascribed the healthy effect of fruit and vegetable consumption mainly to their antioxidant contents, it has bee...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2013.853877
更新日期:2013-12-01 00:00:00
abstract::Carotenoids scavenge simple lipid-like alkylperoxyl radicals. However, the rate constant is too low to be determined directly and the mechanism is likewise not known with certainty [Mortensen, A. and Skibsted, L.H. (1998) FEBS Lett. 426, 392-396]. It is demonstrated that carotenoids react with peroxyl radicals only sl...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760290006501
更新日期:2002-02-01 00:00:00
abstract::Radicals from one-electron oxidation of 4-hydroxycinnamate, ferulate and 3,4-dihydroxycinnamate have been formed by reaction with the oxidising triplet state of duroquinone. All three compounds react with triplet duroquinone with second order rate constants close to the diffusion-controlled limit. The identity of the ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100300621
更新日期:2001-07-01 00:00:00
abstract::Recent research about the role of free radical derivatives of oxygen and nitrogen in biological systems has highlighted the possibility that antioxidants, such as vitamin E, that prevent these processes in vitro may be capable of carrying out a similar function in living organisms in vivo. There is increasing evidence...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715769709097827
更新日期:1997-06-01 00:00:00
abstract::To examine local and systemic oxidative status of lung cancer (LC) and oxidant effects of radiotherapy (RT), this study evaluated antioxidants and markers of oxidative and nitrosative stress in bronchoalveolar lavage (BAL) fluid and in the blood of 36 LC patients and 36 non-cancer controls at baseline and during and a...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760902942824
更新日期:2009-07-01 00:00:00
abstract::Although the primary biological function of retinal photoreceptors is to absorb light and provide visual information, extensive exposure to intense light could increase the risk of phototoxic reactions mediated by products of rhodopsin bleaching that might accumulate in photoreceptor outer segments (POS). The phototox...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2019.1603377
更新日期:2019-04-01 00:00:00
abstract::The interaction between the hydrophobic indolinonic nitroxide radical, 1,2-dihydro-2-methyl-2-phenyl-3H-indole-3-one-1-oxyl and hydrophilic alpha-, beta- and gamma-cyclodextrin derivatives was investigated in water by phase-solubility analysis. Among the studied cyclodextrins, random methyl-beta-cyclodextrin (RM-beta-...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760400023689
更新日期:2005-01-01 00:00:00
abstract::The effect of antioxidants on ammonia induced CNS and renal pathobiology in 10 sheep infested by L. Cuprina larvae was investigated. The condition produces severe dermatitis, proliferation of macrophages and hyperammonaemia, and free radicals may therefore be involved in the pathogenesis. Five of the sheep (treated gr...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769409056553
更新日期:1994-07-01 00:00:00
abstract::α-Tocopherol (a form of vitamin E) is a fat-soluble vitamin that can prevent lipid peroxidation of cell membranes. This antioxidant activity of α-tocopherol can help to prevent cardiovascular disease, atherosclerosis and cancer. We investigated the α-tocopherol level and the expression of α-tocopherol transfer protein...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760310001600426
更新日期:2003-01-01 00:00:00
abstract::Phospholipids (PLs) are important biomolecules that not only constitute structural building blocks and scaffolds of cell and organelle membranes but also play a vital role in cell biochemistry and physiology. Moreover, dietary exogenous PLs are characterised by high nutritional value and other beneficial health effect...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715762.2019.1657573
更新日期:2019-01-01 00:00:00
abstract::Curcumin is a polyphenol derived from the plant Curcuma longa that induces apoptotic cell death in malignant cancer cell lines. It has been shown previously that mitochondrial NADP(+)-dependent isocitrate dehydrogenase (IDPm) plays an essential role in defense against oxidative stress by supplying NADPH for antioxidan...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2010.540574
更新日期:2011-04-01 00:00:00
abstract::Pyruvate is a well-known scavenger of hydrogen peroxide (H2O2). In addition, it scavenges superoxide radical (O2.-). However, evidence on its intracellular antioxidant function is meager at present. Hence, we have examined the effectiveness of this metabolite and its ethyl ester against intracellular oxidative damage ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769809065799
更新日期:1998-02-01 00:00:00
abstract::The seasonal variation of CuCl2-mediated low density lipoprotein (LDL) oxidation (10 microM Cu2+, lag phase, rate of oxidation and maximum absorbance at 234 nm) were measured in 43 men and women on 4-6 occasions (mean 5.7 +/- 0.5) over a 12-month period. The lag phase averaged 52.7 +/- 0.6 min and did not differ by ge...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576031000061020
更新日期:2003-03-01 00:00:00
abstract::Hemopexin, a heme-binding serum glycoprotein, is thought to play an important role in the prevention of oxidative damage that may be catalysed by free heme. Through the use of EPR techniques, the generation of free radicals from organic hydroperoxides by heme and heme-hemopexin complexes, and the concomitant formation...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769509065277
更新日期:1995-12-01 00:00:00