EPR studies on the effects of complexation of heme by hemopexin upon its reactions with organic peroxides.


:Hemopexin, a heme-binding serum glycoprotein, is thought to play an important role in the prevention of oxidative damage that may be catalysed by free heme. Through the use of EPR techniques, the generation of free radicals from organic hydroperoxides by heme and heme-hemopexin complexes, and the concomitant formation of high oxidation-state iron species has been studied; these species are implicated as causative agents in processes such as cardiovascular disease and carcinogenesis. From the rates of production of these species from both n-alkyl and branched hydroperoxides, it has been inferred that the dramatic reduction in the yield of oxidising species generated by heme upon its complexation with hemopexin arises from steric hindrance of the access of hydroperoxide to the bound heme.


Free Radic Res


Free radical research


Timmins GS,Davies MJ,Song DX,Muller-Eberhard U




Has Abstract


1995-12-01 00:00:00












  • Cyanidin-3-O-beta-glucopyranoside protects myocardium and erythrocytes from oxygen radical-mediated damages.

    abstract::The cyanidin-3-O-beta-glucopyranoside (C-3-G) antioxidant capacity towards reactive oxygen species (ROS)-mediated damages was assessed in tissue and cells submitted to increased oxidative stress. In the isolated ischemic and reperfused rat heart, 10 or 30 degreesM C-3-G protected from both lipid peroxidation (66.7 and...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Amorini AM,Lazzarino G,Galvano F,Fazzina G,Tavazzi B,Galvano G

    更新日期:2003-04-01 00:00:00

  • Comparison of the phenolic composition of fruit juices by single step gradient HPLC analysis of multiple components versus multiple chromatographic runs optimised for individual families.

    abstract::After minimal sample preparation, two different HPLC methodologies, one based on a single gradient reversed-phase HPLC step, the other on multiple HPLC runs each optimised for specific components, were used to investigate the composition of flavonoids and phenolic acids in apple and tomato juices. The principal compon...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Bremner PD,Blacklock CJ,Paganga G,Mullen W,Rice-Evans CA,Crozier A

    更新日期:2000-06-01 00:00:00

  • Scavenging of benzylperoxyl radicals by carotenoids.

    abstract::Carotenoids scavenge simple lipid-like alkylperoxyl radicals. However, the rate constant is too low to be determined directly and the mechanism is likewise not known with certainty [Mortensen, A. and Skibsted, L.H. (1998) FEBS Lett. 426, 392-396]. It is demonstrated that carotenoids react with peroxyl radicals only sl...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Mortensen A

    更新日期:2002-02-01 00:00:00

  • Hispidulin: antioxidant properties and effect on mitochondrial energy metabolism.

    abstract::Hispidulin (6-methoxy-5,7,4'-trihydroxyflavone) and eupafolin (6-methoxy-5,7,3',4'-tetrahydroxyflavone), are flavonoids found in the leaves of Eupatorium litoralle. They have recognized antioxidant and antineoplastic properties, although their action mechanisms have not been previously described. We now report the eff...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Dabaghi-Barbosa P,Mariante Rocha A,Franco da Cruz Lima A,Heleno de Oliveira B,Benigna Martinelli de Oliveira M,Gunilla Skare Carnieri E,Cadena SM,Eliane Merlin Rocha M

    更新日期:2005-12-01 00:00:00

  • Influence of an orally effective SOD on hyperbaric oxygen-related cell damage.

    abstract::In a prospective, double-blind, randomised placebo-controlled study, we tested the hypothesis that a new formulation consisting of wheat gliadin chemically combined with a vegetal (thus orally effective) preparation of superoxide dismutase (SOD) allows to prevent hyperbaric oxygen (HBO)-induced oxidative cell stress. ...

    journal_title:Free radical research

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Muth CM,Glenz Y,Klaus M,Radermacher P,Speit G,Leverve X

    更新日期:2004-09-01 00:00:00

  • Will the 'good fairies' please prove to us that vitamin E lessens human degenerative disease?

    abstract::Recent research about the role of free radical derivatives of oxygen and nitrogen in biological systems has highlighted the possibility that antioxidants, such as vitamin E, that prevent these processes in vitro may be capable of carrying out a similar function in living organisms in vivo. There is increasing evidence...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Diplock AT

    更新日期:1997-06-01 00:00:00

  • Lymphocyte antioxidant response and H2O2 production after a swimming session: gender differences.

    abstract::This study evaluated the gender differences in response to intense exercise on lymphocyte hydrogen peroxide production, nitric oxide handling and mitochondrial superoxide dismutase (MnSOD) activity and gene expression. Fifteen males and nine females participated voluntarily in the study and performed a swimming sessio...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Sureda A,Ferrer MD,Tauler P,Tur JA,Pons A

    更新日期:2008-04-01 00:00:00

  • An investigation of the antioxidant activity of black tea using enhanced chemiluminescence.

    abstract::Antioxidants are important species which possess the ability to protect the body from damage caused by free radical-induced oxidative stress. A variety of free radical-scavenging antioxidants exist within the body many of which are derived from dietary sources. There is currently much interest in the antioxidant role ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Robinson EE,Maxwell SR,Thorpe GH

    更新日期:1997-03-01 00:00:00

  • p38 MAPK: a dual role in hepatocyte proliferation through reactive oxygen species.

    abstract::p38 MAPKs are important mediators of signal transduction that respond to a wide range of extracellular stressors such as UV radiation, osmotic shock, hypoxia, pro-inflammatory cytokines, and oxidative stress. The most abundant family member is p38α, which helps to couple cell proliferation and growth in response to ce...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Tormos AM,Taléns-Visconti R,Nebreda AR,Sastre J

    更新日期:2013-11-01 00:00:00

  • APE1/Ref-1 regulates PTEN expression mediated by Egr-1.

    abstract::APE1/Ref-1, the mammalian ortholog of E. coli Xth, and a multifunctional protein possessing both DNA repair and transcriptional regulatory activities, has dual role in controlling cellular response to oxidative stress. It is rate-limiting in repair of oxidative DNA damage including strand breaks and also has co-transc...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Fantini D,Vascotto C,Deganuto M,Bivi N,Gustincich S,Marcon G,Quadrifoglio F,Damante G,Bhakat KK,Mitra S,Tell G

    更新日期:2008-01-01 00:00:00

  • Short term citrus flavonoid supplementation of type II diabetic women: no effect on lipoprotein oxidation tendencies.

    abstract::Flavonoids, which are dietary components and have possible drug uses, inhibit lipoprotein oxidation in vitro. The present study considered whether flavonoid supplementation in humans could influence lipoprotein vulnerability to oxidation. Citrus flavonoid supplementation (about 1g/day, 3 weeks), or placebo, was given ...

    journal_title:Free radical research

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Blostein-Fujii A,DiSilvestro RA,Frid D,Katz C

    更新日期:1999-04-01 00:00:00

  • Diosgenin inhibits superoxide generation in FMLP-activated mouse neutrophils via multiple pathways.

    abstract::Diosgenin possesses anti-inflammatory and anticancer properties. Activated neutrophils produce high concentrations of the superoxide anion which is involved in the pathophysiology of inflammation-related diseases and cancer. In the present study, the inhibitory effect and possible mechanisms of diosgenin on superoxide...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Lin Y,Jia R,Liu Y,Gao Y,Zeng X,Kou J,Yu B

    更新日期:2014-12-01 00:00:00

  • A novel method using 8-hydroperoxy-2'-deoxyguanosine formation for evaluating antioxidative potency.

    abstract::Degenerative diseases such as cancer are induced by oxidative genetic damage. Antioxidants can scavenge reactive oxygen species, but to prevent disease, they must do so quickly, before the DNA bases are damaged. In the present study, a novel method was established for evaluating the potency of antioxidants employing 2...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Sakakibara H,Ashida H,Kanazawa K

    更新日期:2002-03-01 00:00:00

  • Induction of glutathione peroxidase in response to inactivation by nitric oxide.

    abstract::To determine effect of nitric oxide (NO) on cellular glutathione peroxidase (GPX) level in living cells, we measured the activity, protein and mRNA of GPX in rat kidney (KNRK) cells under a high NO condition. Combined treatment of lipopolysaccharide (LPS, 1 microgram/ml) and tumor necrosis factor-alpha (TNF-alpha, 50 ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Dobashi K,Asayama K,Nakane T,Kodera K,Hayashibe H,Nakazawa S

    更新日期:2001-09-01 00:00:00

  • TNF alpha alters mitochondrial membrane potential in L929 but not in TNF alpha-resistant L929.12 cells: relationship with the expression of stress proteins, annexin 1 and superoxide dismutase activity.

    abstract::Tumour necrosis factor alpha (TNF alpha) cytotoxicity is mediated, at least in part, by oxidative stress and phospholipase A2 activation. The first post-receptor events to be observed in TNF alpha-sensitive lines are the generation of superoxide anion (O2-) within the mitochondria and the activation of phospholipase A...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Polla BS,Jacquier-Sarlin MR,Kantengwa S,Mariéthoz E,Hennet T,Russo-Marie F,Cossarizza A

    更新日期:1996-08-01 00:00:00

  • Oxidative damage of rat cerebral cortex and hippocampus, and changes in antioxidative defense systems caused by hyperoxia.

    abstract::In order to elucidate the oxidative damage in rat brain caused by oxidative stress, regional changes in the levels of lipid peroxidation products and antioxidative defense systems in cerebral cortex and hippocampus, and in their synapses, which modulate learning and memory functions in the brain, were studied. When ra...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Onodera K,Omoi NO,Fukui K,Hayasaka T,Shinkai T,Suzuki S,Abe K,Urano S

    更新日期:2003-04-01 00:00:00

  • BSC2 enhances cell resistance to AmB by inhibiting oxidative damage in Saccharomyces cerevisiae.

    abstract::Amphotericin B has been the gold standard for the treatment of invasive mycosis for many years. Its resistance mechanisms are reported to be mainly related to the decrease of ergosterol content or the changes of cell wall. Previous study has shown that Saccharomyces cerevisiae strain lack of BSC2 was sensitive signifi...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Kong Y,Wang Q,Cao F,Zhang X,Fang Z,Shi P,Wang H,Shen Y,Huang Z

    更新日期:2020-04-01 00:00:00

  • Inactivation of heart dihydrolipoamide dehydrogenase by copper Fenton systems. Effect of thiol compounds and metal chelators.

    abstract::Copper Fenton systems (Cu(II)/H2O2 and Cu(II)/Asc) inactivated the lipoamide reductase and enhanced the diaphorase activity of pig-heart lipoamide dehydrogenase (LADH). Cupric ions alone were less effective. As a result of Cu(II)/H2O2 treatment, the number of titrated thiols in LADH decreased from 6 to 1 per subunit. ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Gutierrez-Correa J,Stoppani AO

    更新日期:1995-03-01 00:00:00

  • Hydrogen-rich water improves cognitive impairment gender-dependently in APP/PS1 mice without affecting Aβ clearance.

    abstract::Alzheimer's disease (AD) is characterised as a provoked inflammatory response and oxidative stress along with amyloid β peptide (Aβ) deposition and neurofibrillary tangles in the brain, and effective treatment is greatly needed. Molecular hydrogen, which has been proposed to be an antioxidant that selectively reduces ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Hou C,Peng Y,Qin C,Fan F,Liu J,Long J

    更新日期:2018-12-01 00:00:00

  • Augmented oxidative stress increases 8-oxoguanine preferentially in the transcriptionally active genomic regions.

    abstract::8-Oxoguanine (8-oxoG) is the most common DNA base modification in the mammalian genome, associated with oxidative stress. Here we analysed the alterations in the distribution of 8-oxoG across the entire murine genome, before and after an elevation of oxidative stress by the use of ferric nitrilotriacetate (Fe-NTA) as ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Akatsuka S,Li GH,Kawaguchi S,Takahashi T,Yoshihara M,Suyama M,Toyokuni S

    更新日期:2020-04-16 00:00:00

  • A(1) and A(3) adenosine receptors alter glutathione status in an organ-specific manner and influence the changes after inhibition of gamma-glutamylcysteine ligase.

    abstract::Adenosine levels are increased in stress and act as anti-oxidant and anti-inflammatory mediators by binding to 4 G-protein-coupled receptors. Using genetically modified mice lacking A(1) and A(3) adenosine receptors, treated with ip buthionine-[S,R]-sulphoximine injections to inhibit gamma-glutamylcysteine ligase, the...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Conte C,Grottelli S,Prudenzi E,Bellezza I,Fredholm BB,Minelli A

    更新日期:2009-03-01 00:00:00

  • Oxidative damage of mitochondrial DNA in diabetes and its protection by manganese superoxide dismutase.

    abstract::Retinal mitochondria become dysfunctional in diabetes and the production of superoxide radicals is increased; over-expression of MnSOD abrogates mitochondrial dysfunction and prevents the development of diabetic retinopathy. The mitochondrial DNA (mtDNA) is particularly prone to oxidative damage. The aim of this study...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Madsen-Bouterse SA,Zhong Q,Mohammad G,Ho YS,Kowluru RA

    更新日期:2010-03-01 00:00:00

  • Longitudinal study of circulating oxidized LDL and HDL and fatty liver: the Cardiovascular Risk in Young Finns Study.

    abstract::Oxidative reactions are thought to play a role in the inflammatory condition called fatty liver. It is unclear whether oxidized lipoprotein lipids or proteins are associated with future fatty liver. In the Cardiovascular Risk in Young Finns Study, we determined the circulating levels of LDL and HDL oxidized lipids and...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Kaikkonen JE,Kresanov P,Ahotupa M,Jula A,Mikkilä V,Viikari JS,Juonala M,Hutri-Kähönen N,Kähönen M,Lehtimäki T,Kangas AJ,Soininen P,Ala-Korpela M,Raitakari OT

    更新日期:2016-01-01 00:00:00

  • Free radical oxidation of cholesterol and its precursors: Implications in cholesterol biosynthesis disorders.

    abstract::Free radical oxidation of cholesterol and its precursors contribute significantly to the pathophysiology of a number of human diseases. This review intends to summarize recent developments and provide a perspective on the reactivities of sterols toward free radical oxidation, the free radical reaction mechanism, and t...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Xu L,Porter NA

    更新日期:2015-01-01 00:00:00

  • Knockdown of LINC00473 promotes radiosensitivity of non-small cell lung cancer cells via sponging miR-513a-3p.

    abstract::Non-small cell lung cancer (NSCLC) is the most common form of lung cancer. Radioresistance is a significant obstacle in NSCLC radiotherapy. Long non-coding RNA LINC00473 has been found to impact the radiotherapy in several malignant tumours. This study aimed to investigate the underlying role and mechanism of LINC0047...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Qin P,Li Y,Liu J,Wang N

    更新日期:2020-10-01 00:00:00

  • Effect of pre-exposure of human erythrocytes to oxidants on the haemolytic activity of Sticholysin II. A comparison between peroxynitrite and hypochlorous acid.

    abstract::Stichodactyla heliantus II (St II) is a haemolytic toxin whose activity depends of the characteristics of red blood cells (RBC). Among the factors that may tune the response of the RBC to the toxin activity stand the oxidative status of the cell. This study investigates how pre-oxidation of RBC modifies St II activity...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Celedón G,González G,Lissi E,Cerda T,Bascuñant D,Lepeley M,Pazos F,Lanio ME,Alvarez C

    更新日期:2011-04-01 00:00:00

  • Redox balance following magnetic stimulation training in the quadriceps of patients with severe COPD.

    abstract::In severe COPD patients, oxidative stress, which is involved in their peripheral muscle dysfunction, increases in response to exercise. In this study, muscle oxidative stress was explored after quadriceps magnetic stimulation training. A randomized controlled study was conducted on very severe COPD patients, who under...

    journal_title:Free radical research

    pub_type: 杂志文章,随机对照试验


    authors: Bustamante V,Casanova J,López de Santamaría E,Mas S,Sellarés J,Gea J,Gáldiz JB,Barreiro E

    更新日期:2008-11-01 00:00:00

  • Peroxyl radical scavenging activities of hamamelitannin in chemical and biological systems.

    abstract::The antioxidative activities of hamamelitannin (2',5-di-O-galloyl-hamamelose), gallic acid and dl-alpha-tocopherol against lipid peroxyl radicals were evaluated in chemical and biological systems. The peroxyl radical scavenging activity was evaluated by electron spin resonance (ESR) method in which both spin-trapping ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Masaki H,Atsumi T,Sakurai H

    更新日期:1995-05-01 00:00:00

  • A pilot study to assess the effect of acute exercise on brain glutathione.

    abstract::The brain is highly susceptible to oxidative stress due to its high metabolic demand. Increased oxidative stress and depletion of glutathione (GSH) are observed with aging and many neurological diseases. Exercise training has the potential to reduce oxidative stress in the brain. In this study, nine healthy sedentary ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Rai S,Chowdhury A,Reniers RLEP,Wood SJ,Lucas SJE,Aldred S

    更新日期:2018-01-01 00:00:00

  • Effect of β-carotene on catechol-induced genotoxicity in vitro: evidence of both enhanced and reduced DNA damage.

    abstract::Intake of antioxidants from the diet has been recognized to have beneficial health effects, but the potential benefit of taking antioxidants such as β-carotene as supplements is controversial. The aim of the present study was to evaluate the potential protective effects of a physiologically relevant concentration (2 μ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Åsgård R,Hellman B

    更新日期:2013-09-01 00:00:00