Abstract:
:It has been observed that H9c2 cardiac cells cultured in physiologic solutions exhibit delayed cell death after repeated medium replacements, of which the cause was the relatively mild osmotic challenges during the renewal of the culture medium. Interestingly, the cell damage was associated with altered intracellular GSH homeostasis. Therefore, this study attempted to elucidate the effects of osmotic stress on GSH metabolism. In cells subjected to osmotic stress by lowering the NaCl concentration of the medium, the cell swelling was rapidly counterbalanced, but the intracellular GSH content was significantly lower in 3 h. Meanwhile, the ratio of GSH-to-GSSG was not affected. As expected, osmotic stress also increased the sensitivity to H(2)O(2), which was attributable to the decrease of GSH content. The decrease of GSH content was similarly evident when the synthetic pathways of GSH were blocked by BSO or acivicin. It was concluded that osmotic stress induced the decrease of intracellular GSH content by increased consumption and this loss of GSH rendered the cells susceptible to a subsequent oxidative stress.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Lee JW,Ko YE,Lee IH,Lee HK,Kim HW,Kim YHdoi
10.1080/10715760802691471subject
Has Abstractpub_date
2009-03-01 00:00:00pages
262-71issue
3eissn
1071-5762issn
1029-2470pii
908472562journal_volume
43pub_type
杂志文章abstract::Ascorbic acid (vitamin C) induced hydrogen peroxide (H(2)O(2)) formation was measured in household drinking water and metal supplemented Milli-Q water by using the FOX assay. Here we show that ascorbic acid readily induces H(2)O(2) formation in Cu(II) supplemented Milli-Q water and poorly buffered household drinking w...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500249861
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abstract::The significance of 5-lipoxygenase and myeloperoxidase activities has not been extensively studied among young male smokers. Leukotriene B(4), 20-hydroxy-leukotriene B(4), 20-carboxy-leukotriene B(4) and 3-chlorotyrosine were measured in plasma and urinary samples of young male smokers at 8 hours following cigarette a...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2012.701291
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abstract::Expression of multiple drug resistant (MDR) phenotype and over-expression of P-glycoprotein (P-gp) in the human hepatocellular carcinoma (HCC) cell clone P1(0.5), derived from the PLC/PRF/5 cell line (P5), are associated with strong resistance to oxidative stress and a significant (p < 0.01) increase in intracellular ...
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更新日期:2004-07-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1089987
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abstract::We investigated whether oxidant status and antioxidant enzyme activities during ageing of mouse brain are regulated in sex-dependent manner. In the homogenate from the brain of 1, 4, 10 and 18 months old male and female CBA mice, lipid peroxidation (LPO), total superoxide dismutase (tSOD), catalase (CAT) and glutathio...
journal_title:Free radical research
pub_type: 杂志文章
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abstract::After minimal sample preparation, two different HPLC methodologies, one based on a single gradient reversed-phase HPLC step, the other on multiple HPLC runs each optimised for specific components, were used to investigate the composition of flavonoids and phenolic acids in apple and tomato juices. The principal compon...
journal_title:Free radical research
pub_type: 杂志文章
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journal_title:Free radical research
pub_type: 杂志文章
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abstract::We have previously reported that H2O2-induced apoptosis in HL-60 human leukemia cells takes place in the presence of chloride, requires myeloperoxidase (MPO), and occurs through oxidative reactions involving hypochlorous acid and chloramines. We now report that when chloride is replaced by the pseudohalide thiocyanate...
journal_title:Free radical research
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更新日期:2004-02-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
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更新日期:1999-02-01 00:00:00
abstract::Silibinin is an active constituent extracted from blessed milk thistle (Silybum marianum). Our previous study demonstrated that silibinin induced autophagy and apoptosis via reactive oxygen species (ROS) generation in HeLa cells. In this study, we investigated whether the autophagy- and apoptosis-associated molecules ...
journal_title:Free radical research
pub_type: 杂志文章
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更新日期:2012-03-01 00:00:00
abstract::Ribonucleotide reductases catalyze the irreversible reductive formation of 2'-deoxyribonucleotides required for DNA replication and cell proliferation, and a radical mechanism was assumed to be involved in this reaction. In order to search for a radical in the aerobic manganese ribonucleotide reductase (Mn-RRase) by e...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769609088046
更新日期:1996-06-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章,评审
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更新日期:2000-11-01 00:00:00
abstract::Hypericin and pseudohypericin are polycyclic-phenolic structurally related compounds found in Hypericum perforatum L. (St John's wort). As hypericin has been found to bind to LDL one may assume that it can act as antioxidant of LDL lipid oxidation, a property which is of prophylactic/therapeutic interest regarding ath...
journal_title:Free radical research
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doi:10.1080/10715760600978831
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pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Free radical research
pub_type: 杂志文章,评审
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更新日期:2006-10-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576021000043058
更新日期:2003-03-01 00:00:00
abstract::The aim of this study was to test the hypothesis that pulmonary inflammation and emphysema induced by cadmium (Cd) inhalation are associated with pulmonary oxidative stress. Two groups of Sprague Dawley rats were used: one vehicle-exposed group undergoing inhalation of NaCl (0.9%, n = 24) and one Cd-exposed group unde...
journal_title:Free radical research
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abstract::Thioredoxin-1 (TRX) is a redox-active protein with anti-inflammatory effects. This study investigated the optimal delivery method and the mechanisms of recombinant human TRX (rhTRX) to suppress neutrophil recruitment in a rat bleomycin (BLM)-induced sustained acute lung injury model. In male Wister rats intratracheall...
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更新日期:2010-02-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
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更新日期:2002-04-01 00:00:00
abstract::Ionizing radiations such as X-ray and γ-ray can directly or indirectly produce clustered or multiple damages in DNA. Previous studies have reported that overexpression of DNA glycosylases in Escherichia coli (E. coli) and human lymphoblast cells caused increased sensitivity to γ-ray and X-ray irradiation. However, the...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1061186
更新日期:2015-10-01 00:00:00
abstract::The mechanism of high-grade serous ovarian cancer (HGSC) development remains elusive. This review outlines recent advances in the understanding of sequential molecular changes associated with the development of HGSC, as well as describes oxidative stress-induced genomic instability and carcinogenesis. This article rev...
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pub_type: 杂志文章,评审
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journal_title:Free radical research
pub_type: 杂志文章
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更新日期:1999-05-01 00:00:00
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pub_type: 杂志文章,评审
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abstract::Nerve growth factor (NGF) is one of the several structurally related proteins, named neurotrophins (NTs), that regulate neuronal survival, development, function, and plasticity. Moreover, NGF is an important activator of antioxidant mechanisms. These NGF functions are mediated by tropomyosin-related kinase receptor A ...
journal_title:Free radical research
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