Abstract:
:The oxidative stress possibly resulting from an inherited respiratory chain (RC) deficiency was investigated in a series of human cultured skin fibroblasts presenting either ubiquinone depletion or isolated defect of the various RC complexes. Taken as an index for superoxide overproduction, a significant induction of superoxide dismutase activity was observed in complex V-deficient fibroblasts harboring the NARP-mutation in the ATPase 6 gene. Superoxide dismutase induction was also noticed, albeit to a lesser extent, in complex II-deficient fibroblasts with a mutation in the nuclear gene encoding the flavoprotein subunit of the succinate dehydrogenase. No sign of oxidative stress could be found in ubiquinone-depleted fibroblasts. In all cases but complex IV-defect, increased oxidative stress was associated with increased cell death. In glucose-rich medium, apoptosis appeared as the main cell death process associated with all types of RC defect. However, similar to the great variations in oxidative stress associated with the various types of RC defect, we found that apoptotic features differed noticeably between defects. No indication of increased cell death was found in ubiquinone-depleted fibroblasts.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Geromel V,Rötig A,Munnich A,Rustin Pdoi
10.1080/10715760290021216keywords:
subject
Has Abstractpub_date
2002-04-01 00:00:00pages
375-9issue
4eissn
1071-5762issn
1029-2470journal_volume
36pub_type
杂志文章abstract::Hydroxyl and 1-hydroxyethyl radical adducts of 5,5-dimethylpyrroline N-oxide (DMPO) were prepared by photolysis, and mechanisms for loss of their EPR signals in rat liver microsomal suspensions were evaluated. Rates of NADPH-dependent EPR signal loss were more rapid in phosphate buffer than in Tris buffer. Addition of...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769609149069
更新日期:1996-12-01 00:00:00
abstract::Reactive oxygen species (ROS) are involved in the mechanism of photoaging and carcinogenesis. Skin is endowed with antioxidant enzymes including superoxide dismutases (SOD): cytosolic copper zinc SOD and mitochondrial manganese SOD. The aim of our study was to estimate the protective effect of manganese against oxidat...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769509065255
更新日期:1995-10-01 00:00:00
abstract::In human organism, the gaseous radical molecule nitric oxide (NO) is produced in various cells from L-arginine by the catalytic action of NO synthases (NOS). The metabolic fate of NO includes oxidation to nitrate by oxyhaemoglobin in red blood cells and autoxidation in haemoglobin-free media to nitrite. Nitrate and ni...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760500053651
更新日期:2005-08-01 00:00:00
abstract::Copper Fenton systems (Cu(II)/H2O2 and Cu(II)/Asc) inactivated the lipoamide reductase and enhanced the diaphorase activity of pig-heart lipoamide dehydrogenase (LADH). Cupric ions alone were less effective. As a result of Cu(II)/H2O2 treatment, the number of titrated thiols in LADH decreased from 6 to 1 per subunit. ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769509147543
更新日期:1995-03-01 00:00:00
abstract::Reactive oxygen species (ROS) have been poised at a straddled state of being beneficiary as well detrimental depending on its threshold levels. Maintaining the homeostasis of ROS is imperative for normal cellular physiology, wherein physiological concentrations of ROS are involved in cell signaling and elevated ROS co...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2016.1155708
更新日期:2016-01-01 00:00:00
abstract::Hispidulin (6-methoxy-5,7,4'-trihydroxyflavone) and eupafolin (6-methoxy-5,7,3',4'-tetrahydroxyflavone), are flavonoids found in the leaves of Eupatorium litoralle. They have recognized antioxidant and antineoplastic properties, although their action mechanisms have not been previously described. We now report the eff...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/13561820500177659
更新日期:2005-12-01 00:00:00
abstract::APE1/Ref-1, the mammalian ortholog of E. coli Xth, and a multifunctional protein possessing both DNA repair and transcriptional regulatory activities, has dual role in controlling cellular response to oxidative stress. It is rate-limiting in repair of oxidative DNA damage including strand breaks and also has co-transc...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701765616
更新日期:2008-01-01 00:00:00
abstract::Reperfusion injury of the liver occurs in liver transplantation and in major hepatectomies. It triggers a severe oxidative stress that leads to increased lipid peroxidation. In our study we examined the effect of parenteral supranutritional administration of alpha-tocopherol, a vitamin that plays a key role in the end...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100300641
更新日期:2001-08-01 00:00:00
abstract::Oxidative stress is associated with a range of inflammatory lung diseases including asthma, adult respiratory distress syndrome, idiopathic pulmonary fibrosis, pneumonia, lung transplantation, chronic obstructive pulmonary disease, cystic fibrosis, bronchiectasis and lung cancer. Increased concentrations of reactive o...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760310001623331
更新日期:2003-12-01 00:00:00
abstract::We investigated the effects of fluvastatin, a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, on reactive oxygen species (ROS) and on oxidative DNA damage in vitro, as well as the effects of the main fluvastatin metabolites (M2, M3, and M4) and other inhibitors of the same enzyme, pravastatin and simvastati...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100301291
更新日期:2001-12-01 00:00:00
abstract::Over the last decade, a dual character of cell response to oxidative stress, eustress versus distress, has become increasingly recognized. A growing body of evidence indicates that under physiological conditions, low concentrations of reactive oxygen and nitrogen species (RONS) maintained by the activity of endogenous...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715762.2018.1457217
更新日期:2018-05-01 00:00:00
abstract::We have previously reported that H2O2-induced apoptosis in HL-60 human leukemia cells takes place in the presence of chloride, requires myeloperoxidase (MPO), and occurs through oxidative reactions involving hypochlorous acid and chloramines. We now report that when chloride is replaced by the pseudohalide thiocyanate...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760310001643302
更新日期:2004-02-01 00:00:00
abstract::We studied the effects of a single post whole-body low-dose irradiation (50 cGy of gamma-ray) on mice with ferric nitrilotriacetate (Fe3+-NTA)-induced transient hepatopathy. As a result, low-dose irradiation accelerated the rate of recovery. Based on the changes in glutamic oxaloacetic transaminase (GOT) activities, g...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760000300221
更新日期:2000-03-01 00:00:00
abstract::p38 MAPKs are important mediators of signal transduction that respond to a wide range of extracellular stressors such as UV radiation, osmotic shock, hypoxia, pro-inflammatory cytokines, and oxidative stress. The most abundant family member is p38α, which helps to couple cell proliferation and growth in response to ce...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2013.821200
更新日期:2013-11-01 00:00:00
abstract::Globular adiponectin (gAd) induces the generation of reactive oxygen species (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. This study investigated the role of the mammalian target of rapamycin (mTOR) in gAd-induced ROS and NO generation. gAd stimulation induced phosphorylation of mTOR, which ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715760903348328
更新日期:2010-02-01 00:00:00
abstract::Adriamycin (ADR) is a chemotherapeutic agent useful in treating various cancers. ADR is a quinone-containing anthracycline chemotherapeutic and is known to produce reactive oxygen species (ROS) in heart. Application of this drug can have serious side effects in various tissues, including brain, apart from the known ca...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500143478
更新日期:2005-11-01 00:00:00
abstract::Coenzyme Q10 (CoQ10) is an essential cofactor of the electron transport gene as well as an important antioxidant, which is particularly effective within mitochondria. A number of prior studies have shown that it can exert efficacy in treating patients with known mitochondrial disorders. We investigated the potential u...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760290021315
更新日期:2002-04-01 00:00:00
abstract::Free radical oxidation of cholesterol and its precursors contribute significantly to the pathophysiology of a number of human diseases. This review intends to summarize recent developments and provide a perspective on the reactivities of sterols toward free radical oxidation, the free radical reaction mechanism, and t...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2014.985219
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Measurement of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) has recently become more popular as a means of assessing oxidative stress in the human body. The aim of this study is to compare the levels of urine 8-OHdG in patients with type 2 diabetes with and without nephropathy and to evaluate its role as a b...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2012.710902
更新日期:2012-10-01 00:00:00
abstract::Experimental autoimmune encephalomyelitis (EAE) reproduces a multiple sclerosis (MS)-like experimental model. The main objective was to evaluate the effect of extremely low-frequency electromagnetic fields (EL-EMF) application, like a paradigm of transcranial magnetic stimulation (TMS) in the development of EAE. Rats ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1324955
更新日期:2017-05-01 00:00:00
abstract::Manganese superoxide dismutase (MnSOD) is essential for life as dramatically illustrated by the neonatal lethality of mice that are deficient in MnSOD. In addition, mice expressing only 50% of the normal compliment of MnSOD demonstrate increased susceptibility to oxidative stress and severe mitochondrial dysfunction r...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760100300281
更新日期:2001-04-01 00:00:00
abstract::The turning point between apoptosis and necrosis induced by hydrogen peroxide (H2O2) have been investigated using human T-lymphoma Jurkat cells. Cells treated with 50 microM H2O2 exhibited caspase-9 and caspase-3 activation, finally leading to apoptotic cell death. Treatment with 500 microM H2O2 did not exhibit caspas...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600632552
更新日期:2006-06-01 00:00:00
abstract::Approaching living systems, aqueous solutions are appropriate to characterize antioxidants, whereas the frequently used standard 1,1-diphenyl-2-picrylhydrazyl (DPPH) is insoluble in water. Therefore, mixed water-ethanol solvents were investigated using the electron paramagnetic resonance (EPR) spectroscopy. Two forms ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600930014
更新日期:2007-04-01 00:00:00
abstract::γ-Tocopherol (γ-T) scavenges reactive nitrogen species (RNS) to form 5-NO2-γ-T (NGT). However, α-T supplementation decreases circulating γ-T, which could limit its RNS scavenging activities. We hypothesized that α-T supplementation would mitigate NGT accumulation by impairing γ-T status. Healthy smokers (21 ± 1 y, n =...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1040788
更新日期:2015-01-01 00:00:00
abstract::The present study was designed to determine whether hydroxymethylglutaryl-CoA reductase inhibitors (statins) modulate the NO production via iNOS in adipocytes stimulated by lipopolysaccharide (L) and tumour necrosis factor-alpha (T). Well-differentiated 3T3-L1 adipocytes significantly produced NO by LT-treatment. Pre-...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701534368
更新日期:2007-09-01 00:00:00
abstract::The aim of the study was to investigate whether there is transmembrane transport of intact glutathione ([3H]-GSH, 0.1 microCi) in rat and human type II pneumocytes (T2P), and if this transport might be dependent on the redox state of the extracellular fluid. The T2P were pretreated with acivicin (250 microM) to inhibi...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769900300411
更新日期:1999-05-01 00:00:00
abstract::A study of peroxyl radical-mediated bovine serum albumin oxidation in the presence of the quinolinic aminoxyl 1,2-dihydro-2,2-diphenyl-4-ethoxy-quinoline-1-oxyl (QAO) was carried out in order to test its efficiency as a protein antioxidant. Albumin oxidation was induced by the tert-butylhydroperoxide/PbO2 system. The ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769409056583
更新日期:1994-10-01 00:00:00
abstract::To solve the problems faced in clinical use of hypocrellins, a water-soluble preparation of Hypocrellin B (HB), HB-Triton X-100 (TX-100) micelles, was prepared. To evaluate the photodynamic activity, the free radicals (*OH and HB*-) and singlet oxygen (1O2) generated via photosensitization of the preparation in aqueou...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760310001601056
更新日期:2003-10-01 00:00:00
abstract::Stichodactyla heliantus II (St II) is a haemolytic toxin whose activity depends of the characteristics of red blood cells (RBC). Among the factors that may tune the response of the RBC to the toxin activity stand the oxidative status of the cell. This study investigates how pre-oxidation of RBC modifies St II activity...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2010.536838
更新日期:2011-04-01 00:00:00
abstract::During the early postimplantation period, rodent embryos survive in a relatively anaerobic environment in utero and are vulnerable to a high oxygen pressure. They become resistant to oxygen stress when they are exposed to a higher oxygen pressure after the uteroplacental circulation is established. However, it is unkn...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576021000006626
更新日期:2002-09-01 00:00:00