Abstract:
:Nerve growth factor (NGF) is one of the several structurally related proteins, named neurotrophins (NTs), that regulate neuronal survival, development, function, and plasticity. Moreover, NGF is an important activator of antioxidant mechanisms. These NGF functions are mediated by tropomyosin-related kinase receptor A (TrkA). Although NTs and their receptors have been shown to be expressed in visceral tissues, the extent to which NTs are involved in the physiology of visceral tissues is less clear. NGF is the most expressed NT in adult mouse livers. Although NGF is an important modulator of antioxidant mechanisms in neural tissues, few studies describe the relationship between oxidative stress and NGF expression in the liver. In this study, we demonstrate that ngfb mRNA is positively modulated in mouse livers after oxidative injury via intraperitoneal injection of 14 mg/kg sodium arsenite, 6 mmol/kg L-buthionine-S-R-sulfoximine (BSO), or 300 mg/kg acetaminophen (APAP). In addition to the upregulation of ngfb, we observed the phosphorylation of the NGF high-affinity receptor TrkA in the liver as well as the downstream phosphorylation of Akt, NF-kB nuclear migration and iκbα and tx-1 mRNA upregulation. These effects were abolished when a neutralizing anti-NGF antibody was used. Furthermore, this anti-NGF antibody alone induced oxidative stress in the liver by decreasing the reduced glutathione, increasing the oxidized glutathione, and downregulating tx-1 mRNA. Thus, NGF plays a critical role in liver protection against oxidative stress and xenobiotic injury as well as maintains a reduced thiol state.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Valdovinos-Flores C,Gonsebatt MEdoi
10.3109/10715762.2013.783210subject
Has Abstractpub_date
2013-05-01 00:00:00pages
404-12issue
5eissn
1071-5762issn
1029-2470journal_volume
47pub_type
杂志文章abstract::It is well known that reactive oxygen species (ROS) attack several living tissues and increase the risk of development and progression of serious diseases. In neuronal level, ROS induce cell death in concentration-dependent fashion. However, little is known about the mechanisms of neuronal changes by ROS prior to indu...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2011.647689
更新日期:2012-02-01 00:00:00
abstract::The impact of classic cardiovascular risk factors on oxidative stress status in a high-risk cardiovascular Mediterranean population of 527 subjects was estimated. Oxidative stress markers (malondialdehyde, 8-oxo-7'8'-dihydro-2'-deoxyguanosine, oxidized/reduced glutathione ratio) together with the activity of antioxida...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715760903247231
更新日期:2009-12-01 00:00:00
abstract::The use of antioxidants to prevent intracellular free radical damage is an area currently attracting considerable research interest. The compound 2',7'-dichlorofluorescin diacetate (DCFH-DA) is a probe for intracellular peroxide formation commonly used in such studies. During our studies we unexpectedly found that inc...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709097782
更新日期:1997-01-01 00:00:00
abstract::Radicals from one-electron oxidation of 4-hydroxycinnamate, ferulate and 3,4-dihydroxycinnamate have been formed by reaction with the oxidising triplet state of duroquinone. All three compounds react with triplet duroquinone with second order rate constants close to the diffusion-controlled limit. The identity of the ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100300621
更新日期:2001-07-01 00:00:00
abstract::Lipid peroxidation generates reactive aldehydes, most notably hydroxynonenal (HNE), which covalently binds amino acid residue side chains leading to protein inactivation and insolubility. Specific adducts of lipid peroxidation have been demonstrated to be intimately associated with pathological lesions of Alzheimer's ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2013.794265
更新日期:2013-07-01 00:00:00
abstract::Intake of antioxidants from the diet has been recognized to have beneficial health effects, but the potential benefit of taking antioxidants such as β-carotene as supplements is controversial. The aim of the present study was to evaluate the potential protective effects of a physiologically relevant concentration (2 μ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2013.815346
更新日期:2013-09-01 00:00:00
abstract::The production of microRNAs (miRNA) is influenced by various stimuli, including environmental stresses. We hypothesized that reactive oxygen species (ROS)-associated stress could regulate macrophage miRNA synthesis. miRNAs undergo unique steps of maturation processing through either one of two pathways of cytoplasmic ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2014.974037
更新日期:2015-01-01 00:00:00
abstract::Antioxidants are important species which possess the ability to protect the body from damage caused by free radical-induced oxidative stress. A variety of free radical-scavenging antioxidants exist within the body many of which are derived from dietary sources. There is currently much interest in the antioxidant role ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709097807
更新日期:1997-03-01 00:00:00
abstract::Nine male volunteers were given 36 g of cocoa powder (containing 2610 mg of polyphenols) per day with sugar and 6 volunteers received an equivalent amount of sugar for 2 weeks. Conjugated diene production in LDL induced by 2-2' azobis(4-methoxy-2,4-dimethylvaleronitrile) (V-70) and copper ion were evaluated. The lag t...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100300091
更新日期:2001-01-01 00:00:00
abstract::Anxiety disorders are the most common mental illness in the USA affecting 18% of the population. The cause(s) of anxiety disorders is/are not completely clear, and research in the neurobiology of anxiety at the molecular level is still rather limited. Although mounting clinical and preclinical evidence now indicates t...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715762.2018.1475733
更新日期:2018-07-01 00:00:00
abstract::The effects of various free radicals derived from 6-formylpterin (6-FP), alpha-phenyl-tert-butyl nitrone (PBN) and 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) combined with hyperthermia, on gene expression in similarly enhanced apoptosis of human lymphoma U937 cells were investigated using cDNA microarrays c...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600946432
更新日期:2007-01-01 00:00:00
abstract::There is a correlation between oxidative stress generated by diethylnitrosamine (DEN) metabolism and liver cancer development. Quercetin is a flavonoid with anti-carcinogenic and antioxidant properties. This study demonstrates the mechanism of action for the chemopreventive effect of quercetin. A 10 mg/kg dose of quer...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760802626535
更新日期:2009-02-01 00:00:00
abstract::There is a convincing body of evidence that oxidative stress is involved in the pathogenesis of acute pancreatitis. The effects of different radical scavengers suggested that reactive oxygen metabolites are generated at very early stage of disease and contribute to amplify the pancreatic damage. Oxidative stress is al...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2013.829571
更新日期:2013-11-01 00:00:00
abstract::The effect of antioxidant supplementation on biomarkers of oxidative stress was investigated in a 6-week intervention study in 60 overweight men. The supplement contained a combination of antioxidants aiming to correspond to the antioxidant content found in a diet rich in fruit and vegetables. Placebo, single or doubl...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715761003647101
更新日期:2010-05-01 00:00:00
abstract::The antioxidative activities of hamamelitannin (2',5-di-O-galloyl-hamamelose), gallic acid and dl-alpha-tocopherol against lipid peroxyl radicals were evaluated in chemical and biological systems. The peroxyl radical scavenging activity was evaluated by electron spin resonance (ESR) method in which both spin-trapping ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769509147550
更新日期:1995-05-01 00:00:00
abstract::Tropolones, the naturally occurring compounds responsible for the durability of heartwood of several cupressaceous trees, have been shown to possess both metal chelating and antioxidant properties. However, little is known about the ability of tropolone and its derivatives to protect cultured cells from oxidative stre...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760400017244
更新日期:2005-02-01 00:00:00
abstract::Sublytic levels (microM) of hemin destabilized RBC membrane as indicated by ghost fragmentation pattern using a laser viscodiffractometer. Furthermore, electron microscopic study shows that 5 microM of hemin induced echinocytic transformation whereas higher hemin concentration (40 microM) induced spherocytic transform...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709097838
更新日期:1997-07-01 00:00:00
abstract::Recovery of neurological function in patients following cardiac arrest and cardiopulmonary resuscitation (CPR) is a complex event. Free radical induced oxidative stress is supposed to be involved in this process. We studied levels of 8-iso-PGF2alpha (indicating oxidative injury) and 15-keto-dihydro-PGF2alpha (indicati...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576021000043058
更新日期:2003-03-01 00:00:00
abstract::Retinal mitochondria become dysfunctional in diabetes and the production of superoxide radicals is increased; over-expression of MnSOD abrogates mitochondrial dysfunction and prevents the development of diabetic retinopathy. The mitochondrial DNA (mtDNA) is particularly prone to oxidative damage. The aim of this study...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715760903494168
更新日期:2010-03-01 00:00:00
abstract::We have investigated the toxicity to human monocytemacrophages, and susceptibility to oxidation, of different individual dietary fatty acids in cholesterol esters and triglycerides, added to the cell cultures as coacervates with bovine serum albumin. Toxicity was assessed using release of radioactivity from cells prel...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709097815
更新日期:1997-04-01 00:00:00
abstract::Rabbits were fed a diet containing 1% cholesterol for 8 weeks and the levels of iron and oxidized lipids in liver analysed using atomic absorption spectroscopy and gas chromatography-mass spectrometry. A non-significant trend to an increase in iron level, but significant increases in the lipid peroxidation products, F...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760802691455
更新日期:2009-03-01 00:00:00
abstract::Glucose at pathophysiological concentrations was able to accelerate copper-induced oxidation of isolated low-density lipoprotein (LDL) and whole serum. The efficiency of glucose was favored under the following circumstances: (a) when LDL oxidation was induced by low copper concentration, (b) when LDL was partly oxidiz...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760290025906
更新日期:2002-05-01 00:00:00
abstract::Various lines of evidence indicate that an important part in the pathogenesis of atherosclerosis is the modification of the plasma low-density lipoproteins (LDLs). A large number of pro-inflammatory and pro-atherogenic properties have been ascribed to the oxidatively modified LDLs and their components. There is consid...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2015.1036052
更新日期:2015-01-01 00:00:00
abstract::Oxidatively induced damage caused by free radicals and other DNA-damaging agents generate a plethora of products in the DNA of living organisms. There is mounting evidence for the involvement of this type of damage in the etiology of numerous diseases including carcinogenesis. For a thorough understanding of the mecha...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2015.1014814
更新日期:2015-05-01 00:00:00
abstract::In human organism, the gaseous radical molecule nitric oxide (NO) is produced in various cells from L-arginine by the catalytic action of NO synthases (NOS). The metabolic fate of NO includes oxidation to nitrate by oxyhaemoglobin in red blood cells and autoxidation in haemoglobin-free media to nitrite. Nitrate and ni...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760500053651
更新日期:2005-08-01 00:00:00
abstract::Reactive oxygen species (ROS) have been poised at a straddled state of being beneficiary as well detrimental depending on its threshold levels. Maintaining the homeostasis of ROS is imperative for normal cellular physiology, wherein physiological concentrations of ROS are involved in cell signaling and elevated ROS co...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2016.1155708
更新日期:2016-01-01 00:00:00
abstract::Over the last decades, oxidative stress has been described as a deleterious phenomenon contributing to numerous noncommunicable diseases such as cardiovascular disease, diabetes, and cancers. As many authors ascribed the healthy effect of fruit and vegetable consumption mainly to their antioxidant contents, it has bee...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2013.853877
更新日期:2013-12-01 00:00:00
abstract::Peroxiredoxin 1 (PRDX1) is an antioxidant enzyme that, when secreted, can act as a proinflammatory signal. Here we studied the regulation of intracellular PRDX1 by lipopolysaccharide (LPS) and interferon-gamma (IFN-γ) in the RAW 264.7 mouse macrophage cell line. While LPS or IFN-γ alone did not affect PRDX1 protein le...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2019.1637863
更新日期:2019-08-01 00:00:00
abstract::Although the primary biological function of retinal photoreceptors is to absorb light and provide visual information, extensive exposure to intense light could increase the risk of phototoxic reactions mediated by products of rhodopsin bleaching that might accumulate in photoreceptor outer segments (POS). The phototox...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2019.1603377
更新日期:2019-04-01 00:00:00
abstract::In cells, NADH and NADPH are mainly bound to dehydrogenases such as lactate dehydrogenase (LDH). In cell-free systems, the binary LDH-NADH complex has been demonstrated to produce reactive oxygen species via a chain oxidation of NADH initiated and propagated by superoxide. We studied here whether this chain radical re...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500231786
更新日期:2005-10-01 00:00:00