Abstract:
:Sublytic levels (microM) of hemin destabilized RBC membrane as indicated by ghost fragmentation pattern using a laser viscodiffractometer. Furthermore, electron microscopic study shows that 5 microM of hemin induced echinocytic transformation whereas higher hemin concentration (40 microM) induced spherocytic transformation. In addition, hemin oxidized sulfhydryl groups in a dose dependent fashion and Electron Spin Resonance study suggests that such oxidation may involve a thiyl radical. Moreover, sulfhydryl compounds enhanced hemin-induced lipid peroxidation. Desferroxamine could prevent hemin-induced sulfhydryl oxidation as well as hemin-induced decrease in membrane stability. In contrast, vitamin E could effectively prevent hemin-induced lipid peroxidation but could not prevent hemin-mediated membrane destabilization.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Chiu DT,Huang TY,Hung IJ,Wei JS,Liu TZ,Stern Adoi
10.3109/10715769709097838subject
Has Abstractpub_date
1997-07-01 00:00:00pages
55-62issue
1eissn
1071-5762issn
1029-2470journal_volume
27pub_type
杂志文章abstract::Intracellular levels of H2O2 in BHK-21 cells are not static but decline progressively with cell growth. Exposure of cells to inhibitors of catalase, or glutathione peroxidase, not only diminishes this decline but also depresses rates of cell proliferation, suggesting important growth regulatory roles for those antioxi...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769509065268
更新日期:1995-11-01 00:00:00
abstract::We tested the hypothesis that hypertension associated with polycythemia vera (PV) may be related to hemoglobin released from erythrocytes (cell-free hemoglobin, fHb). We assessed hematocrit, mean arterial pressure (MAP), blood viscosity, and the level of fHb and nitrite/nitrate (NOx) in the plasma of 73 PV patients an...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2013.860225
更新日期:2014-02-01 00:00:00
abstract::The biomimetic model of micelles of linoleic acid containing 2-mercaptoethanol and the antioxidant was examined under gamma irradiation up to 400 Gy in aerobic or deoxygenated conditions where thiyl radicals are the main reactive species. Lipid peroxidation was retarded by ascorbic acid and α-tocopherol, whereas this ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2016.1231401
更新日期:2016-11-01 00:00:00
abstract::Gamma-glutamylcysteine synthetase (GCS; also referred to as glutamate-cysteine ligase, GLCL) catalyzes the rate-limiting reaction in glutathione (GSH) biosynthesis. The GCS holoenzyme is composed of a catalytic and regulatory subunit, each encoded by a unique gene. In addition to some conditions which specifically upr...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760000300291
更新日期:2000-04-01 00:00:00
abstract::Hyperthermia-induced apoptosis and its enhancement in the presence of a temperature-dependent free radical initiator, 2,2'-azobis (2-aminopropane) dihydrochloride (AAPH) were examined in human uterine cervical cancer cell lines, CaSki and HeLa. When both cell lines were treated with hyperthermia at 44 degrees C for 60...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576031000088292
更新日期:2003-06-01 00:00:00
abstract::To define the molecular mechanism(s) of carvedilol inhibition of lipid peroxidation we have utilized model systems that allow us to study the different reactions involved in this complex process. Carvedilol inhibits the peroxidation of sonicated phosphatidylcholine liposomes triggered by FeCl2 addition whereas atenolo...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769800300421
更新日期:1998-11-01 00:00:00
abstract::The oxidative stress possibly resulting from an inherited respiratory chain (RC) deficiency was investigated in a series of human cultured skin fibroblasts presenting either ubiquinone depletion or isolated defect of the various RC complexes. Taken as an index for superoxide overproduction, a significant induction of ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760290021216
更新日期:2002-04-01 00:00:00
abstract::Liver microsomal functions related to xenobiotic biotransformation and free radical production were studied in control rats and in animals subjected to L-3,3',5-triiodothyronine (T3) and/or lindane administration as possible mechanisms contributing to oxidative stress, in relation to the activity of enzymes (superoxid...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769800300051
更新日期:1998-07-01 00:00:00
abstract::The reduction of ferrate(VI) to ferrate(V) by superoxide ions was studied over the pH range 2.6-13.0 using the premix pulse radiolysis technique. The pH dependence indicates that only the unstable protonated forms of ferrate, H2FeO4 (pKa3.5) and HFeO4- (pKa7.3) are reactive, k(HFeO4(-) + O2) = (1.7 +/- 0.2) x 10(7) M-...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769609088016
更新日期:1996-03-01 00:00:00
abstract::The purpose of this double clinical study was (1) to evaluate the effect of one single intake (300 ml) of red wine (RW) on the plasma antioxidant capacity (pAOC) and plasma phenolics over the 24-h time period following the intake, and (2) to compare the long-term effects of daily intakes (250 ml/d) of RW, white wine (...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760310001598097
更新日期:2003-09-01 00:00:00
abstract::We investigated the effects of fluvastatin, a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, on reactive oxygen species (ROS) and on oxidative DNA damage in vitro, as well as the effects of the main fluvastatin metabolites (M2, M3, and M4) and other inhibitors of the same enzyme, pravastatin and simvastati...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100301291
更新日期:2001-12-01 00:00:00
abstract::The turning point between apoptosis and necrosis induced by hydrogen peroxide (H2O2) have been investigated using human T-lymphoma Jurkat cells. Cells treated with 50 microM H2O2 exhibited caspase-9 and caspase-3 activation, finally leading to apoptotic cell death. Treatment with 500 microM H2O2 did not exhibit caspas...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600632552
更新日期:2006-06-01 00:00:00
abstract::Ascorbic acid (vitamin C) induced hydrogen peroxide (H(2)O(2)) formation was measured in household drinking water and metal supplemented Milli-Q water by using the FOX assay. Here we show that ascorbic acid readily induces H(2)O(2) formation in Cu(II) supplemented Milli-Q water and poorly buffered household drinking w...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500249861
更新日期:2005-11-01 00:00:00
abstract::Effects of vitamins B, C, E, K and P, as well as coenzymes Q, on formation of final products of radiation-induced free-radical transformations of ethanol, ethylene glycol, alpha-methylglycoside and glucose in aqueous solutions were studied. Based on the obtained results, it can be concluded that there are substances a...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500147479
更新日期:2005-07-01 00:00:00
abstract::C-phycocyanins, major biliproteins of blue green algae (cyanobacteria), widely used as colourants in food and cosmetics are known for their antioxidant as well as therapeutic potential. Recent claims indicating phycobiliproteins exert stronger photodynamic action on tumor cells than clinically approved hematoporphyrin...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600696938
更新日期:2006-08-01 00:00:00
abstract::APE1/Ref-1, the mammalian ortholog of E. coli Xth, and a multifunctional protein possessing both DNA repair and transcriptional regulatory activities, has dual role in controlling cellular response to oxidative stress. It is rate-limiting in repair of oxidative DNA damage including strand breaks and also has co-transc...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701765616
更新日期:2008-01-01 00:00:00
abstract::Mammalian odorant-binding proteins (OBPs) are soluble lipocalins produced in the nasal mucosa and in other epithelial tissues of several animal species, where they are supposed to serve as scavengers for small structurally unrelated hydrophobic molecules. These would include odorants and toxic aldehydes like 4-hydroxy...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2014.910867
更新日期:2014-07-01 00:00:00
abstract::In human organism, the gaseous radical molecule nitric oxide (NO) is produced in various cells from L-arginine by the catalytic action of NO synthases (NOS). The metabolic fate of NO includes oxidation to nitrate by oxyhaemoglobin in red blood cells and autoxidation in haemoglobin-free media to nitrite. Nitrate and ni...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760500053651
更新日期:2005-08-01 00:00:00
abstract::Hypoxia is a well-known threat to neuronal cells and triggers the pathophysiological syndromes in extreme environments such as high altitudes and traumatic conditions such as stroke. Among several prophylactic molecules proven suitable for ameliorating free radical damage, NAP (an octapeptide with initial amino acids:...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1011153
更新日期:2015-04-01 00:00:00
abstract:INTRODUCTION:Nuclear factor, erythroid 2 like 2 (NFE2L2) is an important transcription factor that protects cells from oxidative stress (OS). NFE2L2 deficiency in placentas is associated with pregnancy complications. We have demonstrated that elevated OS existed in placental shares of the smaller fetus in selective int...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1315113
更新日期:2017-04-01 00:00:00
abstract::We have investigated the toxicity to human monocytemacrophages, and susceptibility to oxidation, of different individual dietary fatty acids in cholesterol esters and triglycerides, added to the cell cultures as coacervates with bovine serum albumin. Toxicity was assessed using release of radioactivity from cells prel...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709097815
更新日期:1997-04-01 00:00:00
abstract::The widely used anti-cancer drug cisplatin imparts various toxic manifestations in the host, with nephrotoxicity being the most severe one. The trace element selenium shows antioxidant activity in both human and animals. The present study was designed to assess the chemoprotecting and chemoenhancing efficacy of a naph...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1012079
更新日期:2015-04-01 00:00:00
abstract::Involvement of oxidative stress is implicated in the progression of complication of diabetes mellitus. With respect to heart diseases, we have studied role of oxidative stress/antioxidants using rats treated with streptozotocin to induce diabetes (DM). Hemodynamic and echocardiographic measurements showed thickening o...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100300231
更新日期:2001-03-01 00:00:00
abstract::We investigated the effect of curcumin on liver injury in diabetic rats induced by streptozotocin (STZ) through modulation of endoplasmic reticulum stress (ERS) and unfolded protein response (UPR). Experimental diabetes was induced by a single intraperitoneal injection of STZ (55 mg/kg), and curcumin was given at 100 ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2014.999674
更新日期:2015-03-01 00:00:00
abstract::Manganese superoxide dismutase (MnSOD) levels have been found to be low in human pancreatic cancer [Pancreas 26, (2003), 23] and human pancreatic cancer cell lines [Cancer Res. 63, (2003), 1297] when compared to normal human pancreas. We hypothesized that stable overexpression of pancreatic cancer cells with MnSOD cDN...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760400017376
更新日期:2004-11-01 00:00:00
abstract::The oxidized intermediates generated upon exposure of Aspergillus niger catalase to hydrogen peroxide and superoxide radical fluxes were examined with UV-visible spectrophotometry. Hydrogen peroxide and superoxide radical were generated by means of glucose/glucose oxidase and xanthine/xanthine oxidase systems. Serial ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769409145624
更新日期:1994-01-01 00:00:00
abstract::In aerobes, oxygen is essential for maintenance of life. However, incomplete reduction of oxygen leads to generation of reactive oxygen species. These oxidants oxidise biological macromolecules present in their vicinity and thereby impair cellular functions causing oxidative stress (OS). Aerobes have evolved both enzy...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715762.2019.1702656
更新日期:2020-01-01 00:00:00
abstract::The preventive effect of 20(S)-ginsenoside Rg(3) (20(S)-Rg(3)) on lipopolysaccharide (LPS)-induced oxidative tissue injury in rats was investigated in this study. The elevated serum nitrite/nitrate, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase and creatinine levels in LPS-treated control rats were ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701581740
更新日期:2007-10-01 00:00:00
abstract::Recovery of neurological function in patients following cardiac arrest and cardiopulmonary resuscitation (CPR) is a complex event. Free radical induced oxidative stress is supposed to be involved in this process. We studied levels of 8-iso-PGF2alpha (indicating oxidative injury) and 15-keto-dihydro-PGF2alpha (indicati...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576021000043058
更新日期:2003-03-01 00:00:00
abstract::Asthma is an allergic inflammation driven by the Th2 immune response with release of cytokines such as IL-4 and IL-13, which contribute to the airflow limitations and airway hyperresponsiveness (AHR). The involvement of oxidative stress in this process is well-established, but the specific role of the superoxide anion...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1364377
更新日期:2017-07-01 00:00:00