Abstract：
:Lipid peroxidation generates reactive aldehydes, most notably hydroxynonenal (HNE), which covalently binds amino acid residue side chains leading to protein inactivation and insolubility. Specific adducts of lipid peroxidation have been demonstrated to be intimately associated with pathological lesions of Alzheimer's disease (AD), suggesting that oxidative stress is a major component in the disease. Here, we examined the HNE-cross-linking modifications by using an antibody specific for a lysine-lysine cross-link. Since in a prior study we noted no immunolabeling of neuritic plaques or neurofibrillary tangles but instead found strong labeling of axons, we focused this study on axons. Axonal labeling was examined in mouse sciatic nerve, and immunoblotting showed the cross-link was restricted to neurofilament heavy and medium subunits, which while altering migration, did not indicate larger NF aggregates, indicative of intermolecular cross-links. Examination of mice at various ages showed the extent of modification remaining relatively constant through the life span. These findings demonstrate lipid-cross-linking peroxidation primarily involves lysine-rich neurofilaments and is restricted to intramolecular cross-links.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Perry EA,Castellani RJ,Moreira PI,Nunomura A,Lui Q,Harris PL,Sayre LM,Szweda PA,Szweda LI,Zhu X,Smith MA,Perry Gdoi
10.3109/10715762.2013.794265subject
Has Abstractpub_date
2013-07-01 00:00:00pages
507-10issue
6-7eissn
1071-5762issn
1029-2470journal_volume
47pub_type
杂志文章abstract：:This study evaluated the gender differences in response to intense exercise on lymphocyte hydrogen peroxide production, nitric oxide handling and mitochondrial superoxide dismutase (MnSOD) activity and gene expression. Fifteen males and nine females participated voluntarily in the study and performed a swimming sessio...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760801989926
更新日期：2008-04-01 00:00:00
abstract：:N-nitroso species have recently been detected in animal tissues. Protein N-nitrosotryptophan is the best candidate for this N-nitroso pool. N-nitrosation of N-blocked trytophan derivatives like melatonin (MelH) by N2O3 or peroxynitrite (ONOOH/ONOO- ) has been observed under conditions of pH and reagent concentrations ...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760600693414
更新日期：2006-09-01 00:00:00
abstract：:Radicals from one-electron oxidation of 4-hydroxycinnamate, ferulate and 3,4-dihydroxycinnamate have been formed by reaction with the oxidising triplet state of duroquinone. All three compounds react with triplet duroquinone with second order rate constants close to the diffusion-controlled limit. The identity of the ...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760100300621
更新日期：2001-07-01 00:00:00
abstract：:Glutathione plays a central role in the maintenance of cellular antioxidant defense. The alterations in the glutathione and associated recyclic enzymes caused by both exercise training and ethanol are well documented; however, their interactive effects with age are not well understood. Therefore, the influence of agei...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760802069462
更新日期：2008-05-01 00:00:00
abstract：:Recent research about the role of free radical derivatives of oxygen and nitrogen in biological systems has highlighted the possibility that antioxidants, such as vitamin E, that prevent these processes in vitro may be capable of carrying out a similar function in living organisms in vivo. There is increasing evidence...
journal_title：Free radical research
pub_type： 杂志文章,评审
doi：10.3109/10715769709097827
更新日期：1997-06-01 00:00:00
abstract：:Transforming growth factor β1 (TGF-β1) induces Mv1Lu cell senescence through inactivating glycogen synthase kinase 3 (GSK3), thereby inactivating complex IV and increasing intracellular ROS. In the present study, we identified protein kinase C delta (PKCδ) as an upstream regulator of GSK3 inactivation in this mechanis...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.3109/10715762.2014.929120
更新日期：2014-09-01 00:00:00
abstract：:Cellular exposure to ionising radiation leads to oxidative stress events, which refer to elevated intracellular levels of reactive oxygen species (ROS). The elevated levels of ROS significantly contributed to γ-radiation (IR) induced cytotoxicity. In an attempt to minimise these cytotoxic effects, antioxidant compound...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715762.2017.1395025
更新日期：2017-12-01 00:00:00
abstract：BACKGROUND:Measurement of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) has recently become more popular as a means of assessing oxidative stress in the human body. The aim of this study is to compare the levels of urine 8-OHdG in patients with type 2 diabetes with and without nephropathy and to evaluate its role as a b...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.3109/10715762.2012.710902
更新日期：2012-10-01 00:00:00
abstract：:The potential of free radical formation in serum of beta-thalassemia/Hb E patients receiving a single oral dose of 25 mg/kg body weight of deferiprone, a bidentate orally active iron chelator, was evaluated using EPR/spin trapping technique. In the presence of ascorbic acid and tert-butylhydroperoxide, EPR signals of ...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760902870611
更新日期：2009-05-01 00:00:00
abstract：:Adriamycin (ADR) is nephrotoxic. One component of ADR-induced nephropathy may be oxidative stress. This study used a recently developed line of transgenic mice (Nmt) on the FVB background strain, which over-express the antioxidant protein metallothionein (MT) in podocytes. Cultured podocytes from Nmt mice were resista...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760802657308
更新日期：2009-02-01 00:00:00
abstract：:Resorcinols (pentadecylresorcinol, olivetol, orcinol and resorcinol) exhibit antioxidant properties in liposomal systems. Antioxidant potency depends on the length of the alkyl chain. Pentadecylresorcinol has been demonstrated to be the most active antioxidant, indicating significance of its alkyl chain in a lipid bil...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.3109/10715769809070804
更新日期：1998-04-01 00:00:00
abstract：:Proteins constitute the major 'working force' for all forms of biological work. Their exact conformation and pattern of folding are tightly connected to their activity and function. Reactive oxygen and nitrogen species (ROS and RNS) are formed during normal metabolism and in higher fluxes under pathological conditions...
journal_title：Free radical research
pub_type： 杂志文章,评审
doi：
更新日期：2000-11-01 00:00:00
abstract：:Intracellular levels of H2O2 in BHK-21 cells are not static but decline progressively with cell growth. Exposure of cells to inhibitors of catalase, or glutathione peroxidase, not only diminishes this decline but also depresses rates of cell proliferation, suggesting important growth regulatory roles for those antioxi...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.3109/10715769509065268
更新日期：1995-11-01 00:00:00
abstract：:Peroxisome proliferator-activated receptor gamma (PPARγ) is known to be activated via exercise-associated transient increases in oxidative stress. However, the precise mechanism(s) triggering PPARγ activation in monocytes during/following exercise remain to be confirmed. Here, two cohorts of five healthy male individu...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.3109/10715762.2014.978311
更新日期：2015-01-01 00:00:00
abstract：:A glycomic approach to the identification of target molecules in glycosyltransferase gene targeting mice is a promising strategy to understand the biological significance of glycosyltransferase genes in vivo. In order to understand the biological effects of N-acetylglucosaminyltransferase III (GnT-III) on tumor format...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/1071576021000005258
更新日期：2002-08-01 00:00:00
abstract：:2,5,7,8-tetramethyl-2-(2'-carboxyethyl)-6-hydroxychroman (alpha-CEHC) has been identified as a major water-soluble metabolite of vitamin E, which circulates in the blood and is excreted with the urine. The aim of this study was to assess the antioxidant activity of alpha-CEHC using several methods with different proox...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/1071576021000005357
更新日期：2002-08-01 00:00:00
abstract：:Validation of markers requires two different steps. The first one is the analytical validation including analytical procedures, definition of standard reference, and round-tests analysis. The second one is the assessment of the biological relevance of the selected marker. This biological relevance may be in the field ...
journal_title：Free radical research
pub_type： 杂志文章,评审
doi：
更新日期：2000-11-01 00:00:00
abstract：:The use of antioxidants to prevent intracellular free radical damage is an area currently attracting considerable research interest. The compound 2',7'-dichlorofluorescin diacetate (DCFH-DA) is a probe for intracellular peroxide formation commonly used in such studies. During our studies we unexpectedly found that inc...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.3109/10715769709097782
更新日期：1997-01-01 00:00:00
abstract：:Increased reactive oxygen species (ROS) and lipid peroxidation may be implicated in the pathogenesis of preeclampsia by causing cell (membrane) damage and impaired endothelial function. Carbonyl derivatives of proteins, or protein carbonyls, may be sensitive biomarkers of ROS-mediated damage. The aim of the study was ...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760000301011
更新日期：2000-11-01 00:00:00
abstract：:The European Standards Committee on Oxidative DNA Damage (ESCODD) was set up in 1997 to resolve methodological problems and to reach agreement on the basal level of 8-oxo-2'-deoxyguanosine (8-oxodG) in biological samples. In the present ESCODD trial 6 samples of 8-oxodG-containing oligonucleotides with different ratio...
journal_title：Free radical research
pub_type： 杂志文章,多中心研究
doi：10.1080/10715760290029047
更新日期：2002-06-01 00:00:00
abstract：:The objective of this study was to elucidate the impact of physical activity during the growth period as well as on oxidative stress and antioxidative potential in adulthood. The experimental animals used were four-week old male Wistar rats, which were randomly divided into three groups. The exercise loads were as fol...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715762.2017.1291939
更新日期：2017-02-01 00:00:00
abstract：:Flavonoids, which are dietary components and have possible drug uses, inhibit lipoprotein oxidation in vitro. The present study considered whether flavonoid supplementation in humans could influence lipoprotein vulnerability to oxidation. Citrus flavonoid supplementation (about 1g/day, 3 weeks), or placebo, was given ...
journal_title：Free radical research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1080/10715769900300341
更新日期：1999-04-01 00:00:00
abstract：:Proteins are subject to various posttranslational modifications, some of them being undesired from the point of view of metabolic efficiency. Prevention of such modifications is expected to provide new means of therapy of diseases and decelerate the process of aging. In this review, modifications of proteins by reacti...
journal_title：Free radical research
pub_type： 杂志文章,评审
doi：10.3109/10715762.2014.953494
更新日期：2014-11-01 00:00:00
abstract：:Epidemiology studies have shown that consumption of fruits and vegetables is associated with the prevention of chronic diseases such as cancer and cardiovascular disease. Induction of cellular phase II detoxifying enzymes is associated with cancer preventive potential. Phenolsulfotransferases (PSTs) are traditionally ...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760500150424
更新日期：2005-08-01 00:00:00
abstract：:The effects of various free radicals derived from 6-formylpterin (6-FP), alpha-phenyl-tert-butyl nitrone (PBN) and 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) combined with hyperthermia, on gene expression in similarly enhanced apoptosis of human lymphoma U937 cells were investigated using cDNA microarrays c...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760600946432
更新日期：2007-01-01 00:00:00
abstract：:We investigated the antioxidant and radical scavenging activity of polyphenolic isochromans. To assess the relation between structure and scavenging properties the natural occurring 1-(3'-methoxy-4'-hydroxy)phenyl-6,7-dihydroxy-isochroman (ISO-3, three OH groups) was compared with three newly synthesized derivatives t...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760500073733
更新日期：2005-05-01 00:00:00
abstract：:We have investigated the toxicity to human monocytemacrophages, and susceptibility to oxidation, of different individual dietary fatty acids in cholesterol esters and triglycerides, added to the cell cultures as coacervates with bovine serum albumin. Toxicity was assessed using release of radioactivity from cells prel...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.3109/10715769709097815
更新日期：1997-04-01 00:00:00
abstract：:The radiation-induced reactions of a water-soluble coumarin derivative, coumarin-3-carboxyl acid (C3CA), have been investigated in aqueous solutions by pulse radiolysis with a 35 MeV electron beam, final product analysis following (60)Co γ-irradiations and deterministic model simulations. Pulse radiolysis revealed tha...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.3109/10715762.2012.684879
更新日期：2012-07-01 00:00:00
abstract：:An increasing incidence of unresponsiveness to antimonials in Leishmaniasis has led to identification of plant-derived anti-leishmanial compounds like Artemisinin. Since iron-mediated generation of free radicals sustains the anti-malarial activity of Artemisinin, this study investigated whether similar mechanisms acco...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.3109/10715762.2010.498475
更新日期：2010-11-01 00:00:00
abstract：:α-Tocopherol (a form of vitamin E) is a fat-soluble vitamin that can prevent lipid peroxidation of cell membranes. This antioxidant activity of α-tocopherol can help to prevent cardiovascular disease, atherosclerosis and cancer. We investigated the α-tocopherol level and the expression of α-tocopherol transfer protein...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760310001600426
更新日期：2003-01-01 00:00:00
