Vitamin C (ascorbic acid) induced hydroxyl radical formation in copper contaminated household drinking water: role of bicarbonate concentration.


:We have previously shown that Vitamin C (ascorbic acid) can trigger hydroxyl radical formation in copper contaminated household drinking water. We report here that the capacity of ascorbic acid to catalyze hydroxyl radical generation in the drinking water samples is strongly dependent on the bicarbonate concentration (buffer capacity and pH) of the samples. We found that at least 50 mg/l bicarbonate was required in the water samples to maintain the pH over 5.0 after ascorbic acid addition. At this pH, that is higher than the pKa1 4.25 of ascorbic acid, a hydroxyl radical generating redox cycling reaction involving the mono-anion of vitamin C and copper could take place. The ascorbic acid induced hydroxyl radical generating reaction could easily be mimicked in Milli-Q water by supplementing the water with copper and bicarbonate. Our results demonstrate that ascorbic acid can induce a pH dependent hydroxyl radical generating reaction in copper contaminated household tap water that is buffered with bicarbonate. The impact of consuming ascorbic acid together with copper and bicarbonate containing drinking water on human health is discussed.


Free Radic Res


Free radical research


Jansson PJ,Asplund KU,Mäkelä JC,Lindqvist C,Nordström T





Has Abstract


2003-08-01 00:00:00












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    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Akatsuka S,Li GH,Kawaguchi S,Takahashi T,Yoshihara M,Suyama M,Toyokuni S

    更新日期:2020-04-16 00:00:00

  • Protein oxidative damage and redox imbalance induced by ionising radiation in CHO cells.

    abstract::Reactive oxygen species (ROS) are important mediators of the cytotoxicity induced by the direct reaction of ionising radiation (IR) with all critical cellular components, such as proteins, lipids, and nucleic acids. The derived oxidative damage may propagate in exposed tissues in a dose- and spatiotemporal dependent m...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Pietraforte D,Paulicelli E,Patrono C,Gambardella L,Scorza G,Testa A,Fattibene P

    更新日期:2018-04-01 00:00:00

  • Inhibiting ROS-TFE3-dependent autophagy enhances the therapeutic response to metformin in breast cancer.

    abstract::Autophagy modulation is a potential therapeutic strategy for breast cancer, and a previous study indicated that metformin exhibits significant anti-carcinogenic activity. However, the ability of metformin to induce autophagy and its role in breast cancer cell death remains unclear. In this study, we exposed MCF-7 cell...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Tan M,Wu A,Liao N,Liu M,Guo Q,Yi J,Wang T,Huang Y,Qiu B,Zhou W

    更新日期:2018-08-01 00:00:00

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    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Pei R,Mah E,Leonard SW,Traber MG,Bruno RS

    更新日期:2015-01-01 00:00:00

  • Differential response of lymphocytes and neutrophils to high intensity physical activity and to vitamin C diet supplementation.

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    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Tauler P,Aguiló A,Gimeno I,Noguera A,Agustí A,Tur JA,Pons A

    更新日期:2003-09-01 00:00:00

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    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Fantini D,Vascotto C,Deganuto M,Bivi N,Gustincich S,Marcon G,Quadrifoglio F,Damante G,Bhakat KK,Mitra S,Tell G

    更新日期:2008-01-01 00:00:00

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    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Kamio N,Akifusa S,Yamashita Y

    更新日期:2011-03-01 00:00:00

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    journal_title:Free radical research

    pub_type: 杂志文章


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    更新日期:2011-05-01 00:00:00

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    abstract::The capacity of non-illuminated nephrotoxin orellanine ([2,2'-bipyridine]-3,3',4,4'-tetrol-1,1'-dioxide) to induce DNA damage in the presence of ferrous iron and dioxygen has been evaluated. Maximal single-strand breaks in plasmid DNA were obtained with a metal to ligand ratio 1:3. Instantaneous oxidation of Fe2+ in p...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Cantin-Esnault D,Oubrahim H,Richard JM

    更新日期:2000-08-01 00:00:00

  • Synthesis and spin trapping properties of polystyrene supported trifluoromethylated cyclic nitrones.

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    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Earla A,Walter ED,Braslau R

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    pub_type: 杂志文章


    authors: Ghosh P,Singha Roy S,Basu A,Bhattacharjee A,Bhattacharya S

    更新日期:2015-04-01 00:00:00

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    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Varfolomeeva EY,Semenova EV,Sokolov AV,Aplin KD,Timofeeva KE,Vasilyev VB,Filatov MV

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    abstract::An involvement of oxidative stress (OS) was found in recent studies of Sjøgren's syndrome (SS) that reported significant changes in protein oxidation, myeloperoxidase activity, TNF-α, nitrotyrosine, and GSH levels in plasma from SS patients. Excess levels of OS markers, as oxidative DNA damage and propanoyl-lysine, we...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Pagano G,Castello G,Pallardó FV

    更新日期:2013-02-01 00:00:00

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    abstract::To determine effect of nitric oxide (NO) on cellular glutathione peroxidase (GPX) level in living cells, we measured the activity, protein and mRNA of GPX in rat kidney (KNRK) cells under a high NO condition. Combined treatment of lipopolysaccharide (LPS, 1 microgram/ml) and tumor necrosis factor-alpha (TNF-alpha, 50 ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Dobashi K,Asayama K,Nakane T,Kodera K,Hayashibe H,Nakazawa S

    更新日期:2001-09-01 00:00:00

  • Antioxidative activity of hydroxylamines. ESR spectra of radicals derived from hydroxylamines.

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    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Nishiyama T,Tamekuni H,Tachibana T,Kitamura H,Saito S

    更新日期:2001-12-01 00:00:00

  • Characterization of hydrogen peroxide and superoxide degrading pathways of Aspergillus niger catalase: a steady-state analysis.

    abstract::The oxidized intermediates generated upon exposure of Aspergillus niger catalase to hydrogen peroxide and superoxide radical fluxes were examined with UV-visible spectrophotometry. Hydrogen peroxide and superoxide radical were generated by means of glucose/glucose oxidase and xanthine/xanthine oxidase systems. Serial ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Lardinois OM,Rouxhet PG

    更新日期:1994-01-01 00:00:00

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    abstract::The metabolism of paraquat generates oxygen radicals. Paraquat has thus been suggested as a model compound to induce oxidative damage to DNA, lipids and proteins in different cells and tissues, although experimental data are inconsistent. In order to explore the possibilities for an animal model of oxidative DNA damag...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Sørensen M,Loft S

    更新日期:2000-05-01 00:00:00

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    abstract::We evaluated whether nutritional vitamin A deficiency generates oxidative stress and inflammation in aorta. Wistar male rats (21 days old) were given free access to a control (8 mg retinol as retinyl palmitate/kg) or a vitamin A- deficient diet for three months. One group of deficient animals was fed with the control ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Gatica L,Alvarez S,Gomez N,Zago MP,Oteiza P,Oliveros L,Gimenez MS

    更新日期:2005-06-01 00:00:00

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    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Wei YP,Jia CN,Lan Y,Hou XQ,Zuo JJ,Cui H,Guan XJ,Wang Y,Mao GY

    更新日期:2019-04-01 00:00:00

  • Nitric oxide (•NO) generation but not ROS plays a major role in silibinin-induced autophagic and apoptotic death in human epidermoid carcinoma A431 cells.

    abstract::Silibinin, a major active constituent of silymarin, is clinically used as a hepatoprotectant, and in recent years, it has been used for the treatment of cancer in China. Because the mechanism of silibinin action on cancer cells was still unclear, we investigated the contribution of silibinin to the induction of apopto...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Yu Y,Fan SM,Yuan SJ,Tashiro S,Onodera S,Ikejima T

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  • Malondialdehyde and 4-hydroxy-2-nonenal in plant tissue cultures: LC-MS determination of 2,4-dinitrophenylhydrazone derivatives.

    abstract::The cytologically active secondary lipid peroxidation products, malondialdehyde (MDA) and 4-hydroxy-2-nonenal (HNE) have been detected as their 2,4-dinitrophenylhydrazone (DNP) derivatives in plant tissue cultures using LC-MS. This paper reports, for the first time, the use of LC-MS methodology to definitively identif...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Deighton N,Magill WJ,Bremner DH,Benson EE

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    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Santos MR,Mira L

    更新日期:2004-09-01 00:00:00

  • Role of thioredoxin peroxidase in aging of stationary cultures of Saccharomyces cerevisiae.

    abstract::A soluble protein from Saccharomyces cerevisiae acts as a peroxidase but requires a NADPH-dependent thioredoxin system and was named thioredoxin peroxidase (TPx). The role of TPx in aging of stationary cultures of S. cerevisiae was investigated in a wild-type strain and a mutant yeast strain in which the tsa gene that...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Lee JH,Park JW

    更新日期:2004-03-01 00:00:00

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    abstract::Adriamycin (ADR) is a chemotherapeutic agent useful in treating various cancers. ADR is a quinone-containing anthracycline chemotherapeutic and is known to produce reactive oxygen species (ROS) in heart. Application of this drug can have serious side effects in various tissues, including brain, apart from the known ca...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Joshi G,Sultana R,Tangpong J,Cole MP,St Clair DK,Vore M,Estus S,Butterfield DA

    更新日期:2005-11-01 00:00:00

  • Scutellarin ameliorates nonalcoholic fatty liver disease through the PPARγ/PGC-1α-Nrf2 pathway.

    abstract::Nonalcoholic fatty liver disease (NAFLD) is characterised by excessive accumulation of hepatic lipids and oxidative injury of hepatocytes. Scutellarin is a flavonoid glycoside having antioxidative stress activity. Our current study aims to investigate the molecular mechanism of scutellarin ameliorating NAFLD. Scutella...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Zhang X,Ji R,Sun H,Peng J,Ma X,Wang C,Fu Y,Bao L,Jin Y

    更新日期:2018-02-01 00:00:00

  • Peroxyl radical scavenging activities of hamamelitannin in chemical and biological systems.

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    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Masaki H,Atsumi T,Sakurai H

    更新日期:1995-05-01 00:00:00

  • PKCδ phosphorylation is an upstream event of GSK3 inactivation-mediated ROS generation in TGF-β1-induced senescence.

    abstract::Transforming growth factor β1 (TGF-β1) induces Mv1Lu cell senescence through inactivating glycogen synthase kinase 3 (GSK3), thereby inactivating complex IV and increasing intracellular ROS. In the present study, we identified protein kinase C delta (PKCδ) as an upstream regulator of GSK3 inactivation in this mechanis...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Byun HO,Jung HJ,Kim MJ,Yoon G

    更新日期:2014-09-01 00:00:00

  • Vitamin E-coated filter decreases levels of free 4-hydroxyl-2-nonenal during haemodialysis sessions.

    abstract::Uraemic subjects undergoing chronic haemodialysis show increased oxidative stress. The use of non-biocompatible filters and reduced antioxidative defences are important sources of reactive oxygen species (ROS) release. The highly oxidative environment accelerates the onset and progression of tissue damage and atherosc...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Odetti P,Traverso N,Monacelli F,Menini S,Vazzana J,Tasso B,Pronzato MA,Robaudo C,Deferrari G

    更新日期:2006-02-01 00:00:00

  • LOX-1 and ROS, inseparable factors in the process of endothelial damage.

    abstract::Lectin-like oxidized low-density lipoprotein (LOX-1) has been identified in endothelial cells as the main receptor of oxidized low-density lipoprotein (OxLDL). LOX-1 is upregulated in the presence of pathological conditions including atherosclerosis, hypertension, and diabetes because it acts as a mediator of "endothe...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Lubrano V,Balzan S

    更新日期:2014-08-01 00:00:00

  • Salicylate trapping of .OH as a tool for studying post-ischemic oxidative injury in the isolated rat heart.

    abstract::The use of salicylate as a chemical trap for .OH represents a simple and convenient alternative to the use of spin trapping techniques to study oxidative injury in isolated perfused organs. In these systems, salicylate is included in the perfusion buffer at concentrations ranging from 0.1 to 2 mM depending on the dete...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Powell SR

    更新日期:1994-11-01 00:00:00