Adaptation to multiday ozone exposure is associated with a sustained increase of bronchoalveolar uric acid.


:The phenomenon of ozone tolerance is described, but the underlying mechanisms remain unknown. We tested whether adaptation to multiday ozone exposure was related to an upregulated pulmonary antioxidant defence. Six calves were exposed to 0.75 ppm ozone, 12 h day(-1) for seven consecutive days. Pulmonary function tests and bronchoalveolar lavage (BAL) were performed before, after the first (D1), third (D3) and seventh (D7) exposure. Differential cell count, total proteins, 8-epi-PGF2alpha, glutathione and uric acid were determined in BAL. Dynamic lung compliance and arterial oxygen tension were significantly decreased and lung oedema impaired pulmonary function on D1. By repeating ozone exposures, progressive functional adaptation occurred. Ozone induced a significant increase of BAL neutrophil percentage on D1. On D3 and D7, neutrophil percentage was progressively decreased, but remained significantly elevated. BAL total proteins were significantly increased on D1 and decreased progressively until D7. 8-Epi-PGF2alpha was significantly increased on D1 and was returned to baseline on D3 and D7, whilst glutathione significantly increased on D3 and returned to baseline on D7. Uric acid was increased ten-fold on D1. On D3, uric acid was increased six-fold and was persistently elevated at D7. This study suggests that ozone adaptation of functional and inflammatory variables is accompanied with sustained BAL uric acid elevation.


Free Radic Res


Free radical research


Kirschvink N,Fiévez L,Bureau F,Degand G,Maghuin-Rogister G,Smith N,Art T,Lekeux P





Has Abstract


2002-01-01 00:00:00












  • GSK-3β inhibition by curcumin mitigates amyloidogenesis via TFEB activation and anti-oxidative activity in human neuroblastoma cells.

    abstract::The translocation of transcription factor EB (TFEB) to the nucleus plays a pivotal role in the regulation of basic cellular processes, such as lysosome biogenesis and autophagy. Autophagy is an intracellular degradation system that delivers cytoplasmic constituents to the lysosome, which is important in maintaining ce...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Song HC,Chen Y,Chen Y,Park J,Zheng M,Surh YJ,Kim UH,Park JW,Yu R,Chung HT,Joe Y

    更新日期:2020-07-28 00:00:00

  • Scavenging of benzylperoxyl radicals by carotenoids.

    abstract::Carotenoids scavenge simple lipid-like alkylperoxyl radicals. However, the rate constant is too low to be determined directly and the mechanism is likewise not known with certainty [Mortensen, A. and Skibsted, L.H. (1998) FEBS Lett. 426, 392-396]. It is demonstrated that carotenoids react with peroxyl radicals only sl...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Mortensen A

    更新日期:2002-02-01 00:00:00

  • Investigation on the antioxidation of the flavonoids based on the photoinduced chemiluminescence of lucigenin by a new Zinc(II) phthalocyanine compound.

    abstract::A zinc(II) phthalocyanine compound, tetra-alpha-(2,2,4-tirmethyl-3-pentoxy) Phthalocyanine Zinc (ZnPc(OR)(4)) was synthesized in this paper and this zinc (II) phthalocyanine compound was used as the photosensitizer in the photoinduced chemiluminescence (PCL) of lucigenin in N,N-dimethylformamide (DMF). The photoexcite...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Wang J,Jiang Z,Huang L,Huang J,Chen G

    更新日期:2008-02-01 00:00:00

  • PCR-verified microarray analysis and functional in vitro studies indicate a role of alpha-tocopherol in vesicular transport.

    abstract::Global gene expression profiles of livers from mice, fed diets differing in alpha-tocopherol content, were compared using DNA microarray technology. Three hundred and eighty nine genes were found to significantly differ in their expression level by a factor of 2 or higher between the high and the low alpha-tocopherol ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Nell S,Bahtz R,Bossecker A,Kipp A,Landes N,Bumke-Vogt C,Halligan E,Lunec J,Brigelius-Flohé R

    更新日期:2007-08-01 00:00:00

  • Resveratrol treatment protects against doxorubicin-induced cardiotoxicity by alleviating oxidative damage.

    abstract::The possible protective effects of resveratrol (RVT) against cardiotoxicity were investigated in Wistar albino rats treated with saline, saline+doxorubicin (DOX; 20 mg/kg) or RVT (10 mg/kg)+DOX. Blood pressure and heart rate were recorded on the 1st week and on the 7th week, while cardiomyopathy was assessed using tra...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Tatlidede E,Sehirli O,Velioğlu-Oğünc A,Cetinel S,Yeğen BC,Yarat A,Süleymanoğlu S,Sener G

    更新日期:2009-03-01 00:00:00

  • Antioxidant properties of catechins and proanthocyanidins: effect of polymerisation, galloylation and glycosylation.

    abstract::A range of catechins and oligomeric procyanidins was purified by high performance liquid chromatography (HPLC) from grape seed, apple skin, lentil and almond flesh. Catechins, galloylated epicatechin, glycosylated catechin, procyanidin dimers, galloylated dimers, trimer, and tetramer species were all identified, purif...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Plumb GW,De Pascual-Teresa S,Santos-Buelga C,Cheynier V,Williamson G

    更新日期:1998-10-01 00:00:00

  • Do mitochondria make nitric oxide? no?

    abstract::Several papers have claimed that mitochondria contain nitric oxide synthase (NOS) and make nitric oxide (NO*) in amounts sufficient to affect mitochondrial respiration. However, we found that the addition of L-arginine or the NOS inhibitor L-NMMA to intact rat liver mitochondria did not have any effect on the respirat...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Tay YM,Lim KS,Sheu FS,Jenner A,Whiteman M,Wong KP,Halliwell B

    更新日期:2004-06-01 00:00:00

  • Hormones and oxidative stress: an overview.

    abstract::In aerobes, oxygen is essential for maintenance of life. However, incomplete reduction of oxygen leads to generation of reactive oxygen species. These oxidants oxidise biological macromolecules present in their vicinity and thereby impair cellular functions causing oxidative stress (OS). Aerobes have evolved both enzy...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Chainy GBN,Sahoo DK

    更新日期:2020-01-01 00:00:00

  • Estimation of hydroxyl radical generation by salicylate hydroxylation method in kidney of mice exposed to ferric nitrilotriacetate and potassium bromate.

    abstract::Hydroxyl radical (*OH) generation in the kidney of mice treated with ferric nitrilotriacetate (Fe-NTA) or potassium bromate (KBrO3) in vivo was estimated by the salicylate hydroxylation method, using the optimal experimental conditions we recently reported. Induction of DNA lesions and lipid peroxidation in the kidney...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Ueno S,Kashimoto T,Susa N,Shiho K,Seki T,Ito N,Takeda-Homma S,Nishimura Y,Sugiyama M

    更新日期:2007-11-01 00:00:00

  • TNF alpha alters mitochondrial membrane potential in L929 but not in TNF alpha-resistant L929.12 cells: relationship with the expression of stress proteins, annexin 1 and superoxide dismutase activity.

    abstract::Tumour necrosis factor alpha (TNF alpha) cytotoxicity is mediated, at least in part, by oxidative stress and phospholipase A2 activation. The first post-receptor events to be observed in TNF alpha-sensitive lines are the generation of superoxide anion (O2-) within the mitochondria and the activation of phospholipase A...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Polla BS,Jacquier-Sarlin MR,Kantengwa S,Mariéthoz E,Hennet T,Russo-Marie F,Cossarizza A

    更新日期:1996-08-01 00:00:00

  • Comparison of 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels using mass spectrometer and urine albumin creatinine ratio as a predictor of development of diabetic nephropathy.

    abstract:BACKGROUND:Measurement of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) has recently become more popular as a means of assessing oxidative stress in the human body. The aim of this study is to compare the levels of urine 8-OHdG in patients with type 2 diabetes with and without nephropathy and to evaluate its role as a b...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Serdar M,Sertoglu E,Uyanik M,Tapan S,Akin K,Bilgi C,Kurt I

    更新日期:2012-10-01 00:00:00

  • Reactivity of an indolinonic aminoxyl with superoxide anion and hydroxyl radicals.

    abstract::The increasing knowledge on the participation of free radicals in many diverse clinical and pathological conditions, has consequently expanded the search for new and versatile antioxidants aimed at combating oxidative stress. Our interest in this field concerns aromatic indolinonic aminoxyls (nitroxides) which efficie...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Damiani E,Carloni P,Stipa P,Greci L

    更新日期:1999-08-01 00:00:00

  • Late-intervention study with ebselen in an experimental model of type 1 diabetic nephropathy.

    abstract:BACKGROUND AND AIM:Previous studies have shown that preventive treatment with the antioxidant, ebselen, in experimental models of type 1 diabetic nephropathy resulted in an attenuation of structural and functional damage in the kidney. However, evidence for the effectiveness of ebselen in late-intervention studies is l...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Tan SM,Sharma A,Stefanovic N,de Haan JB

    更新日期:2015-03-01 00:00:00

  • Placental NFE2L2 is discordantly activated in monochorionic twins with selective intrauterine growth restriction and possibly regulated by hypoxia.

    abstract:INTRODUCTION:Nuclear factor, erythroid 2 like 2 (NFE2L2) is an important transcription factor that protects cells from oxidative stress (OS). NFE2L2 deficiency in placentas is associated with pregnancy complications. We have demonstrated that elevated OS existed in placental shares of the smaller fetus in selective int...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Wu J,He Z,Gao Y,Zhang G,Huang X,Fang Q

    更新日期:2017-04-01 00:00:00

  • New insight on the relationship between LDL composition, associated proteins, oxidative resistance and preparation procedure.

    abstract::Oxidized low density lipoprotein (LDL) plays an important role in atherogenesis. It is generally thought that LDL is mainly oxidized in the intima of vessel walls, surrounded by hydrophilic antioxidants and proteins such as albumin. The aim of this study was to investigate the possible interrelationships between oxida...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Carbonneau MA,Cartron E,Leger CL,Senglat C,Descomps B

    更新日期:2002-02-01 00:00:00

  • Hispidulin: antioxidant properties and effect on mitochondrial energy metabolism.

    abstract::Hispidulin (6-methoxy-5,7,4'-trihydroxyflavone) and eupafolin (6-methoxy-5,7,3',4'-tetrahydroxyflavone), are flavonoids found in the leaves of Eupatorium litoralle. They have recognized antioxidant and antineoplastic properties, although their action mechanisms have not been previously described. We now report the eff...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Dabaghi-Barbosa P,Mariante Rocha A,Franco da Cruz Lima A,Heleno de Oliveira B,Benigna Martinelli de Oliveira M,Gunilla Skare Carnieri E,Cadena SM,Eliane Merlin Rocha M

    更新日期:2005-12-01 00:00:00

  • Hemin-induced membrane sulfhydryl oxidation: possible involvement of thiyl radicals.

    abstract::Sublytic levels (microM) of hemin destabilized RBC membrane as indicated by ghost fragmentation pattern using a laser viscodiffractometer. Furthermore, electron microscopic study shows that 5 microM of hemin induced echinocytic transformation whereas higher hemin concentration (40 microM) induced spherocytic transform...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Chiu DT,Huang TY,Hung IJ,Wei JS,Liu TZ,Stern A

    更新日期:1997-07-01 00:00:00

  • The Cu/Zn superoxide dismutase +35A/C (rs2234694) variant correlates with altered levels of protein carbonyls and glutathione and associates with severity of COPD in a Tunisian population.

    abstract::Chronic obstructive pulmonary disease (COPD) is a major cause of mortality that has been associated with inflammation and oxidative stress. The purpose of the present case-control study was to determine the relationships between oxidative stress-related genetic variants and the risk and severity of COPD, as well as, t...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Ben Anes A,Ben Nasr H,Garrouche A,Bchir S,Dhaouefi Z,Chabchoub E,Tabka Z,Chahed K

    更新日期:2019-03-01 00:00:00

  • The role of tetrahydrobiopterin in superoxide generation from eNOS: enzymology and physiological implications.

    abstract::Tetrahydrobiopterin (BH4) is a ubiquitous pteridine metabolite that serves as a NOS cofactor. Recently, we showed that BH4 efficiently inhibits superoxide generation from the heme group at the oxygenase domain of eNOS. This role indicates that BH4 acts as a redox switch in the catalytic mechanism of the enzyme, which ...

    journal_title:Free radical research

    pub_type: 杂志文章,评审


    authors: Vásquez-Vivar J,Kalyanaraman B,Martásek P

    更新日期:2003-02-01 00:00:00

  • a-Tocopherol Content and a-Tocopherol Transfer Protein Expression in Leukocytes of Children with Acute Leukemia.

    abstract::α-Tocopherol (a form of vitamin E) is a fat-soluble vitamin that can prevent lipid peroxidation of cell membranes. This antioxidant activity of α-tocopherol can help to prevent cardiovascular disease, atherosclerosis and cancer. We investigated the α-tocopherol level and the expression of α-tocopherol transfer protein...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Misaki K,Takitani K,Ogihara T,Inoue A,Kawakami C,Kuno T,Kawamura N,Miyake M,Nakagawa T,Tamai H

    更新日期:2003-01-01 00:00:00

  • The main components of St John's Wort inhibit low-density lipoprotein atherogenic modification: a beneficial "side effect" of an OTC antidepressant drug?

    abstract::Hypericin and pseudohypericin are polycyclic-phenolic structurally related compounds found in Hypericum perforatum L. (St John's wort). As hypericin has been found to bind to LDL one may assume that it can act as antioxidant of LDL lipid oxidation, a property which is of prophylactic/therapeutic interest regarding ath...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Laggner H,Schreier S,Hermann M,Exner M,Mühl A,Gmeiner BM,Kapiotis S

    更新日期:2007-02-01 00:00:00

  • BSC2 enhances cell resistance to AmB by inhibiting oxidative damage in Saccharomyces cerevisiae.

    abstract::Amphotericin B has been the gold standard for the treatment of invasive mycosis for many years. Its resistance mechanisms are reported to be mainly related to the decrease of ergosterol content or the changes of cell wall. Previous study has shown that Saccharomyces cerevisiae strain lack of BSC2 was sensitive signifi...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Kong Y,Wang Q,Cao F,Zhang X,Fang Z,Shi P,Wang H,Shen Y,Huang Z

    更新日期:2020-04-01 00:00:00

  • Visualising reactive oxygen species in live mammals and revealing of ROS-related system.

    abstract::Of all the aerobic respiration by-products, cytotoxic superoxide derived from mitochondrial-leaked electrons, is the only one known to be disposed of intracellularly. Is this fate the only destiny for mitochondrial-leaked electrons? When Cynomolgus monkeys were injected intravenously with reactive oxygen species (ROS)...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Guo J,Xu H,Liu S,Wang Z,Dai Y,Lu J,Zheng S,Xu D,Zhou J,Ke L,Cheng X,Xu M,Zhang X,Guo Y,Lin Y,Ding W,Gao G,Wang H,Chen Q,Yu X,Chen H,Qin L,Sun X,Li Z,Zheng S,Wang J,Cheng Y,Qiu S,Hu Y,Huang P,Li

    更新日期:2019-12-01 00:00:00

  • Oxidative stress, d-ROMs test, and ceruloplasmin.

    abstract::Human serum samples were evaluated for oxidative stress with the d-ROMs test. The ceruloplasmin (CP) and copper contents of the samples was independently measured and compared to those calculated on the basis of the d-ROMs test results for pure CP solutions. The d-ROMs readings did not show any significant correlation...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Colombini F,Carratelli M,Alberti A

    更新日期:2016-01-01 00:00:00

  • Peptides as antioxidants and carbonyl quenchers in biological model systems.

    abstract::Subjecting selected peptides to in vitro analyses covering their ability to interfere with the lipid oxidation chain reaction as well as to protect proteins from direct and indirect oxidation has provided the basis for a more detailed understanding of peptide-mediated protection in biological systems. The efficiency o...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Jongberg S,Carlsen CU,Skibsted LH

    更新日期:2009-10-01 00:00:00

  • Peroxidase-mediated oxidation, a possible pathway for activation of the fungal nephrotoxin orellanine and related compounds. ESR and spin-trapping studies.

    abstract::Orellanine is the tetrahydroxylated and di-N-oxidized bipyridine toxin extracted from several Cortinarius mushrooms among them C. orellanus. The pathogenic mechanism involved in the C. orellanus-poisoning by orellanine leading to kidney impairment is not yet fully understood until now. Electron spin resonance (ESR) sp...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Oubrahim H,Richard JM,Cantin-Esnault D

    更新日期:1998-05-01 00:00:00

  • Phosphate increases mitochondrial reactive oxygen species release.

    abstract::The effects of inorganic phosphate (Pi), the main intracellular membrane permeable anion capable of altering mitochondrial pH gradients (Delta pH), were measured on mitochondrial H2O2 release. As expected, Pi decreased Delta pH and increased the electric membrane potential (Delta Psi). Mitochondrial H2O2 release was s...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Oliveira GA,Kowaltowski AJ

    更新日期:2004-10-01 00:00:00

  • The influence of antioxidants in the thiyl radical induced lipid peroxidation and geometrical isomerization in micelles of linoleic acid.

    abstract::The biomimetic model of micelles of linoleic acid containing 2-mercaptoethanol and the antioxidant was examined under gamma irradiation up to 400 Gy in aerobic or deoxygenated conditions where thiyl radicals are the main reactive species. Lipid peroxidation was retarded by ascorbic acid and α-tocopherol, whereas this ...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Tartaro Bujak I,Mihaljević B,Ferreri C,Chatgilialoglu C

    更新日期:2016-11-01 00:00:00

  • DNA strand scission by the nephrotoxin [2,2'-bipyridine]-3,3',4,4'-tetrol-1,1'-dioxide and related compounds in the presence of iron.

    abstract::The capacity of non-illuminated nephrotoxin orellanine ([2,2'-bipyridine]-3,3',4,4'-tetrol-1,1'-dioxide) to induce DNA damage in the presence of ferrous iron and dioxygen has been evaluated. Maximal single-strand breaks in plasmid DNA were obtained with a metal to ligand ratio 1:3. Instantaneous oxidation of Fe2+ in p...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Cantin-Esnault D,Oubrahim H,Richard JM

    更新日期:2000-08-01 00:00:00

  • Elevated oxidative stress, iron accumulation around microvessels and increased 4-hydroxynonenal immunostaining in zone 1 of the liver acinus in hypercholesterolemic rabbits.

    abstract::Rabbits were fed a diet containing 1% cholesterol for 8 weeks and the levels of iron and oxidized lipids in liver analysed using atomic absorption spectroscopy and gas chromatography-mass spectrometry. A non-significant trend to an increase in iron level, but significant increases in the lipid peroxidation products, F...

    journal_title:Free radical research

    pub_type: 杂志文章


    authors: Ong WY,Jenner AM,Pan N,Ong CN,Halliwell B

    更新日期:2009-03-01 00:00:00