Abstract:
:To determine effect of nitric oxide (NO) on cellular glutathione peroxidase (GPX) level in living cells, we measured the activity, protein and mRNA of GPX in rat kidney (KNRK) cells under a high NO condition. Combined treatment of lipopolysaccharide (LPS, 1 microgram/ml) and tumor necrosis factor-alpha (TNF-alpha, 50 ng/ml) synergistically enhanced (23-folds) nitrite production from KNRK cells. This was suppressed by an inducible NO synthase (iNOS) inhibitor (aminoguanidine, N-nitro-L-arginine methylester hydrochloride) and arginase. iNOS expression was detected by RT-PCR in the treated cells. GPX was inactivated irreversibly when the cells had been homogenized before exposure to a NO donor, S-nitroso-N-acetylpenicillamine (SNAP). In living KNRK cells, SNAP and LPS + TNF-alpha exerted a transient effect on the GPX activity. The treatment with SNAP (200 microM) or sodium nitroprusside (200 microM) enhanced GPX gene expression, which was blocked by a NO scavenger, 2-phenyl-4,4,5,5,-tetramethylimidazoline-1-oxyl-3-oxide. GPX mRNA was markedly increased by the treatment with LPS + TNF-alpha, and aminoguanidine blocked the effect. In cells metabolically labeled with 75Se, LPS + TNF-alpha accelerated the incorporation of radioactivity into GPX molecule by 2.1-fold. These results suggest that inactivation of GPX by NO triggers a signal for inducing GPX gene expression in KNRK cells, thereby restoring the intracellular level of this indispensable enzyme.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Dobashi K,Asayama K,Nakane T,Kodera K,Hayashibe H,Nakazawa Sdoi
10.1080/10715760100300851keywords:
subject
Has Abstractpub_date
2001-09-01 00:00:00pages
319-27issue
3eissn
1071-5762issn
1029-2470journal_volume
35pub_type
杂志文章abstract::Nitronyl nitroxides (NNs) are the paramagnetic probes that are capable of scavenging physiologically relevant reactive oxygen (ROS) and nitrogen (RNS) species, namely superoxide, nitric oxide (NO), and nitroxyl (HNO). NNs are increasingly considered as potent antioxidants and potential therapeutic agents. Understandin...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1013951
更新日期:2015-01-01 00:00:00
abstract::During the early postimplantation period, rodent embryos survive in a relatively anaerobic environment in utero and are vulnerable to a high oxygen pressure. They become resistant to oxygen stress when they are exposed to a higher oxygen pressure after the uteroplacental circulation is established. However, it is unkn...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576021000006626
更新日期:2002-09-01 00:00:00
abstract::This study evaluated the gender differences in response to intense exercise on lymphocyte hydrogen peroxide production, nitric oxide handling and mitochondrial superoxide dismutase (MnSOD) activity and gene expression. Fifteen males and nine females participated voluntarily in the study and performed a swimming sessio...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760801989926
更新日期:2008-04-01 00:00:00
abstract::Oxidative DNA damage pathogenically links to some major diseases. This study aimed to comprehensively assess the association between serum total cholesterol (TC) and oxidative DNA damage based on propensity score matching (PSM) method. A total of 407 participants chronically exposed to arsenic via drinking water from ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2019.1595613
更新日期:2019-04-01 00:00:00
abstract::Microcystin-LR (MCLR) is a potent hepatotoxin. Oxidative stress is thought to be implicated in the cytotoxicity of MCLR, but the mechanisms by which MCLR produces reactive oxygen species (ROS) are still unclear. This study investigated the role and possible sources of ROS generation in MCLR-induced cytogenotoxicity in...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701704599
更新日期:2007-12-01 00:00:00
abstract::The aim of the study was to analyse the effect of Val 16Ala polymorphism in SOD2 gene on oxidative stress parameters and lipid profile of the blood during a three-month wrestling training. The study included 53 Polish young wrestlers. Blood samples were collected at the beginning of the programme and following three m...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1327716
更新日期:2017-05-01 00:00:00
abstract::The turning point between apoptosis and necrosis induced by hydrogen peroxide (H2O2) have been investigated using human T-lymphoma Jurkat cells. Cells treated with 50 microM H2O2 exhibited caspase-9 and caspase-3 activation, finally leading to apoptotic cell death. Treatment with 500 microM H2O2 did not exhibit caspas...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600632552
更新日期:2006-06-01 00:00:00
abstract::G72 has been characterised as a susceptibility gene that can have wide-ranging effects in a number of neurodegenerative diseases, including schizophrenia and major depression. Indeed, its product, pLG72, is a potential serum biomarker for schizophrenia. Previous transcriptomic and biochemical studies have indicated th...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2018.1504293
更新日期:2018-09-01 00:00:00
abstract::We investigated whether oxidant status and antioxidant enzyme activities during ageing of mouse brain are regulated in sex-dependent manner. In the homogenate from the brain of 1, 4, 10 and 18 months old male and female CBA mice, lipid peroxidation (LPO), total superoxide dismutase (tSOD), catalase (CAT) and glutathio...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576031000102178
更新日期:2003-07-01 00:00:00
abstract::The intestinal tract, known for its capability for self-renew, represents the first barrier of defence between the organism and its luminal environment. The thiol/disulfide redox systems comprising the glutathione/glutathione disulfide (GSH/GSSG), cysteine/cystine (Cys/CySS) and reduced and oxidized thioredoxin (Trx/T...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2011.611509
更新日期:2011-11-01 00:00:00
abstract::Oxidative reactions are thought to play a role in the inflammatory condition called fatty liver. It is unclear whether oxidized lipoprotein lipids or proteins are associated with future fatty liver. In the Cardiovascular Risk in Young Finns Study, we determined the circulating levels of LDL and HDL oxidized lipids and...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1133906
更新日期:2016-01-01 00:00:00
abstract::Tumour necrosis factor alpha (TNF alpha) cytotoxicity is mediated, at least in part, by oxidative stress and phospholipase A2 activation. The first post-receptor events to be observed in TNF alpha-sensitive lines are the generation of superoxide anion (O2-) within the mitochondria and the activation of phospholipase A...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769609149917
更新日期:1996-08-01 00:00:00
abstract::Oxidatively induced damage caused by free radicals and other DNA-damaging agents generate a plethora of products in the DNA of living organisms. There is mounting evidence for the involvement of this type of damage in the etiology of numerous diseases including carcinogenesis. For a thorough understanding of the mecha...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2015.1014814
更新日期:2015-05-01 00:00:00
abstract::The use of antioxidants to prevent intracellular free radical damage is an area currently attracting considerable research interest. The compound 2',7'-dichlorofluorescin diacetate (DCFH-DA) is a probe for intracellular peroxide formation commonly used in such studies. During our studies we unexpectedly found that inc...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709097782
更新日期:1997-01-01 00:00:00
abstract::Recent research about the role of free radical derivatives of oxygen and nitrogen in biological systems has highlighted the possibility that antioxidants, such as vitamin E, that prevent these processes in vitro may be capable of carrying out a similar function in living organisms in vivo. There is increasing evidence...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715769709097827
更新日期:1997-06-01 00:00:00
abstract::Brain ischemia and the following reperfusion are important causes for brain damage and leading causes of brain morbidity and human mortality. Numerous observations exist describing the neuronal damage during ischemia/reperfusion, but the outcome of such conditions towards glial cells still remains to be elucidated. Mi...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600978807
更新日期:2007-02-01 00:00:00
abstract::Gamma-glutamylcysteine synthetase (GCS; also referred to as glutamate-cysteine ligase, GLCL) catalyzes the rate-limiting reaction in glutathione (GSH) biosynthesis. The GCS holoenzyme is composed of a catalytic and regulatory subunit, each encoded by a unique gene. In addition to some conditions which specifically upr...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760000300291
更新日期:2000-04-01 00:00:00
abstract::It is well known that antioxidants such as AA (reduced ascorbate), glutathione (GSH) (reduced glutathione) and melatonin can delay seed ageing. Can they recover aged seed? Artificial aged maize seeds were obtained and their reduced germination rate (GR) and high lipid peroxidation were recorded. Exogenous melatonin wa...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1375099
更新日期:2017-10-01 00:00:00
abstract::The synproportionation reaction between ferryl leghemoglobin and oxyleghemoglobin does not occur, at least under conditions where this process could be clearly demonstrated with myoglobin and hemoglobin. In contrast, a cross synproportionation can occur between oxyleghemoglobin and ferryl myoglobin or between ferryl l...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709097848
更新日期:1997-08-01 00:00:00
abstract::The antioxidative activities of hamamelitannin (2',5-di-O-galloyl-hamamelose), gallic acid and dl-alpha-tocopherol against lipid peroxyl radicals were evaluated in chemical and biological systems. The peroxyl radical scavenging activity was evaluated by electron spin resonance (ESR) method in which both spin-trapping ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769509147550
更新日期:1995-05-01 00:00:00
abstract:BACKGROUND:The objective of the present study was to determine whether single administration of the antioxidant enzyme bovine superoxide dismutase (bSOD) after radiation therapy (RT) mitigates development of pulmonary toxicity in rats. METHODS:Female F344 rats (n = 60) were divided among six experimental groups: (1) R...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1066501
更新日期:2015-10-01 00:00:00
abstract::Ischemia-reperfusion injury (IRI) is a leading cause of acute kidney injury (AKI), which contributes to the development of chronic kidney disease (CKD). IRI-induced AKI releases proinflammatory cytokines (e.g. IL-1β, TNF-α, IL-6) that induce a systemic inflammatory response, resulting in proinflammatory cells recruitm...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2019.1668936
更新日期:2019-10-01 00:00:00
abstract:INTRODUCTION:Nuclear factor, erythroid 2 like 2 (NFE2L2) is an important transcription factor that protects cells from oxidative stress (OS). NFE2L2 deficiency in placentas is associated with pregnancy complications. We have demonstrated that elevated OS existed in placental shares of the smaller fetus in selective int...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1315113
更新日期:2017-04-01 00:00:00
abstract::Reactive oxygen species (ROS) released from polymorphonuclear leukocytes and macrophages could cause DNA damage, but also induce cell death. Therefore inhibition of cell death must be an important issue for accumulation of genetic changes in lymphoid cells in inflammatory foci. Scavengers in the post culture medium of...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760000300601
更新日期:2000-07-01 00:00:00
abstract::Epidemiology studies have shown that consumption of fruits and vegetables is associated with the prevention of chronic diseases such as cancer and cardiovascular disease. Induction of cellular phase II detoxifying enzymes is associated with cancer preventive potential. Phenolsulfotransferases (PSTs) are traditionally ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500150424
更新日期:2005-08-01 00:00:00
abstract::The antioxidant properties of protoporphyrin IX and related tetrapyrroles are poorly characterized. Therefore, eight tetrapyrroles, five of which are produced in vivo, were tested to assess their antioxidant capacities in the Salmonella reverse mutation, TEAC, FRAP and ORAC assays. Tertiary-butyl hydroperoxide (tert-B...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2012.715371
更新日期:2012-11-01 00:00:00
abstract::To define the molecular mechanism(s) of carvedilol inhibition of lipid peroxidation we have utilized model systems that allow us to study the different reactions involved in this complex process. Carvedilol inhibits the peroxidation of sonicated phosphatidylcholine liposomes triggered by FeCl2 addition whereas atenolo...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769800300421
更新日期:1998-11-01 00:00:00
abstract::Hydroxyl and 1-hydroxyethyl radical adducts of 5,5-dimethylpyrroline N-oxide (DMPO) were prepared by photolysis, and mechanisms for loss of their EPR signals in rat liver microsomal suspensions were evaluated. Rates of NADPH-dependent EPR signal loss were more rapid in phosphate buffer than in Tris buffer. Addition of...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769609149069
更新日期:1996-12-01 00:00:00
abstract::Validation of markers requires two different steps. The first one is the analytical validation including analytical procedures, definition of standard reference, and round-tests analysis. The second one is the assessment of the biological relevance of the selected marker. This biological relevance may be in the field ...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:
更新日期:2000-11-01 00:00:00
abstract::Many epidemiological data indicate the presence of a strong familial component of longevity that is largely determined by genetics, and a number of possible associations between longevity and allelic variants of genes have been described. A breakthrough strategy to get insight into the genetics of longevity is the stu...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760600917136
更新日期:2006-12-01 00:00:00