Abstract:
:Guanidinosuccinic acid (GSA) is noted for its nitric oxide (NO) mimicking actions such as vasodilatation and activation of the N-methyl-D-aspartate (NMDA) receptor. We have reported that GSA is the product of argininosuccinate (ASA) and some reactive oxygen species, mainly the hydroxyl radical. We tested for GSA synthesis in the presence of NO donors. ASA (1 mM) was incubated with NOR-2, NOC-7 or 3-morpholinosydomine hydrochloride (SIN-1) at 37 degrees C. GSA was determined by HPLC using a cationic resin for separation and phenanthrenequinone as an indicator. Neither NOR-2 or NOC-7 formed GSA. SIN-1, on the other hand, generates NO and the superoxide anion which, in turn, generated peroxynitrite which was then converted to the hydroxyl radical. Incubation of ASA with SIN-1 leads, via this route, to GSA. When ASA was incubated with 1 mM SIN-1, the amount of GSA produced depended on the incubation time and the concentration of ASA. Among the tested SIN-1 concentrations, from 0.5 to 5 mM, GSA synthesis was maximum at 0.5 mM and decreased with increasing concentrations of SIN-1. Carboxy-PTIO, a NO scavenger, completely inhibited GSA synthesis. SOD, a superoxide scavenger, decreased GSA synthesis by 20%, and catalase inhibited GSA synthesis only by 12%; DMSO, a hydroxyl radical scavenger completely inhibited GSA synthesis in the presence of SIN-1. These data suggest that the hydroxyl radical derived from a combination of NO and the superoxide anion generates GSA, a stable NO mimic. Meanwhile, synthesis of GSA by NO produces reactive oxygen and activates the NMDA receptor that generates NO from GSA, suggesting a positive feed back mechanism.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Aoyagi K,Akiyama K,Shahrzad S,Tomida C,Hirayama A,Nagase S,Takemura K,Koyama A,Ohba S,Narita Mdoi
10.1080/10715769900300601keywords:
subject
Has Abstractpub_date
1999-07-01 00:00:00pages
59-65issue
1eissn
1071-5762issn
1029-2470journal_volume
31pub_type
杂志文章abstract::Cholesterol is an essential component of mammalian plasma membrane. Alterations in sterol metabolism or oxidation have been linked to various pathological conditions, including cardiovascular diseases, cancer, and neurodegenerative disorders. Unsaturated sterols are vulnerable to oxidation induced by singlet oxygen an...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2021.1879387
更新日期:2021-01-26 00:00:00
abstract::Paraquat is a synthetic bipyridylium salt widely used as herbicide and defoliant. Enzyme-catalyzed redoxcycling of paraquat generates oxygen radicals. The toxic, even lethal, effects of paraquat are due to free radical-mediated tissue injury. Ocular lesions, sometimes quite severe, have been observed following acciden...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769509064020
更新日期:1995-07-01 00:00:00
abstract::Parkinson's disease (PD) is a neurodegenerative disorder characterised by selective degeneration of the nigral dopaminergic neurons, and neuroinflammation and oxidative stress are believed to be involved in its pathogenesis. In the present study, we provide data that the synthetic steroid exemestane, which is currentl...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1353688
更新日期:2017-06-01 00:00:00
abstract::Transforming growth factor-β1 (TGF-β1) induces expression of the proinflammatory and profibrotic cytokine monocyte chemoattractant protein-1 (MCP-1) in tubular epithelial cells (TECs) and thereby contributes to the tubular epithelial-mesenchymal transition (EMT), which in turn leads to the progression of tubulointerst...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1038258
更新日期:2015-01-01 00:00:00
abstract::The purpose of the present study was to investigate the protective effect of esculetin (ES) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the lung epithelial A549 cells. Mice were intragastrically administered with ES (20 and 40 mg/kg) 1 h prior to LPS challenge. ES pretreatment at doses of 20 and 40...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1087643
更新日期:2015-01-01 00:00:00
abstract::We investigated whether oxidant status and antioxidant enzyme activities during ageing of mouse brain are regulated in sex-dependent manner. In the homogenate from the brain of 1, 4, 10 and 18 months old male and female CBA mice, lipid peroxidation (LPO), total superoxide dismutase (tSOD), catalase (CAT) and glutathio...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576031000102178
更新日期:2003-07-01 00:00:00
abstract::Global gene expression profiles of livers from mice, fed diets differing in alpha-tocopherol content, were compared using DNA microarray technology. Three hundred and eighty nine genes were found to significantly differ in their expression level by a factor of 2 or higher between the high and the low alpha-tocopherol ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701416988
更新日期:2007-08-01 00:00:00
abstract::Reactive oxygen species (ROS) are involved in the mechanism of photoaging and carcinogenesis. Skin is endowed with antioxidant enzymes including superoxide dismutases (SOD): cytosolic copper zinc SOD and mitochondrial manganese SOD. The aim of our study was to estimate the protective effect of manganese against oxidat...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769509065255
更新日期:1995-10-01 00:00:00
abstract::Soy sauce is a traditional fermented seasoning in Asian countries, that has high antioxidant activity in vitro and some antioxidant activity in vivo. We attempted to identify the major antioxidants present, using the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay as a guide. 3-Hydroxy-2-methyl-4H-p...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760601110871
更新日期:2007-04-01 00:00:00
abstract::We have previously reported that H2O2-induced apoptosis in HL-60 human leukemia cells takes place in the presence of chloride, requires myeloperoxidase (MPO), and occurs through oxidative reactions involving hypochlorous acid and chloramines. We now report that when chloride is replaced by the pseudohalide thiocyanate...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760310001643302
更新日期:2004-02-01 00:00:00
abstract::Individuals with low serum cholesterol experience greater than expected age-adjusted mortality from non-atherosclerotic diseases, including cancer, respiratory and digestive illnesses, but the basis for these associations remains unclear. The current investigation considered the hypothesis that hypocholesterolemia is ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769609149049
更新日期:1996-09-01 00:00:00
abstract::Adenosine is an endogenous cardioprotective substance. The present study examines whether exogenous adenosine attenuates cardiac injury induced by oxidative stress. Rat hearts (Langendorff model) were perfused with H2O2 (180 microM) for 10 min, then recovered for 60 min (n = 10). In other groups adenosine 55 microM, 1...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769609087997
更新日期:1996-01-01 00:00:00
abstract::Using a novel phosphorylated spin trap, 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO), an analogue of the commonly used spin trap 5,5'-dimethyl-1-pyrroline N-oxide (DMPO), we have investigated the production of oxygen radical species under illumination of thylakoids from wheat (Triticum durum Desf cv. O...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715769900301511
更新日期:1999-12-01 00:00:00
abstract::The brain is highly susceptible to oxidative stress due to its high metabolic demand. Increased oxidative stress and depletion of glutathione (GSH) are observed with aging and many neurological diseases. Exercise training has the potential to reduce oxidative stress in the brain. In this study, nine healthy sedentary ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1411594
更新日期:2018-01-01 00:00:00
abstract::The impact of classic cardiovascular risk factors on oxidative stress status in a high-risk cardiovascular Mediterranean population of 527 subjects was estimated. Oxidative stress markers (malondialdehyde, 8-oxo-7'8'-dihydro-2'-deoxyguanosine, oxidized/reduced glutathione ratio) together with the activity of antioxida...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715760903247231
更新日期:2009-12-01 00:00:00
abstract::Oxidative stress is a major pathogenetic event occurring in several liver disorders and is a major cause of liver damage due to Ischemia/Reperfusion (I/R) during liver transplantation. While several markers of chronic oxidative stress are well known, early protein targets of oxidative injury are not well defined. In o...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760400029603
更新日期:2005-03-01 00:00:00
abstract::Adriamycin (ADR) is nephrotoxic. One component of ADR-induced nephropathy may be oxidative stress. This study used a recently developed line of transgenic mice (Nmt) on the FVB background strain, which over-express the antioxidant protein metallothionein (MT) in podocytes. Cultured podocytes from Nmt mice were resista...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760802657308
更新日期:2009-02-01 00:00:00
abstract::Rabbits were fed a diet containing 1% cholesterol for 8 weeks and the levels of iron and oxidized lipids in liver analysed using atomic absorption spectroscopy and gas chromatography-mass spectrometry. A non-significant trend to an increase in iron level, but significant increases in the lipid peroxidation products, F...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760802691455
更新日期:2009-03-01 00:00:00
abstract::Transforming growth factor β1 (TGF-β1) induces Mv1Lu cell senescence through inactivating glycogen synthase kinase 3 (GSK3), thereby inactivating complex IV and increasing intracellular ROS. In the present study, we identified protein kinase C delta (PKCδ) as an upstream regulator of GSK3 inactivation in this mechanis...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2014.929120
更新日期:2014-09-01 00:00:00
abstract::6-Hydroxydopamine (6-OHDA) is a neurotoxin to produce an animal model of Parkinson's disease. 6-OHDA increased the formation of 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodG), a biomarker of oxidatively damaged DNA, and induced apoptosis in human neuroblastoma SH-SY5Y cells. Iron or copper chelators inhibited 6-OHDA-i...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760802270334
更新日期:2008-07-01 00:00:00
abstract::Nitration-induced protein damage in the placenta leads to impaired blood flow and deficient feto-placental exchange in diabetic pregnancies. This work studied the effect of nitric oxide and peroxynitrite on Cu/Zn SOD activity in rat placentas and evaluated whether Cu/Zn SOD is nitrated in the placenta from diabetic ra...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2010.512039
更新日期:2010-12-01 00:00:00
abstract::p38 MAPKs are important mediators of signal transduction that respond to a wide range of extracellular stressors such as UV radiation, osmotic shock, hypoxia, pro-inflammatory cytokines, and oxidative stress. The most abundant family member is p38α, which helps to couple cell proliferation and growth in response to ce...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2013.821200
更新日期:2013-11-01 00:00:00
abstract::Flavonoids are components of fruit and vegetables that may be beneficial in the prevention of disease such as cancer and cardiovascular diseases. Their beneficial effects will be dependent upon their uptake and disposition in tissues and cells. The metabolism and pharmacokinetics of flavonoids has been an area of acti...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760410001727858
更新日期:2004-08-01 00:00:00
abstract:BACKGROUND:The objective of the present study was to determine whether single administration of the antioxidant enzyme bovine superoxide dismutase (bSOD) after radiation therapy (RT) mitigates development of pulmonary toxicity in rats. METHODS:Female F344 rats (n = 60) were divided among six experimental groups: (1) R...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1066501
更新日期:2015-10-01 00:00:00
abstract::Previous studies have shown that glucose-6-phosphate dehydrogenase (G6PD)-deficient cells are under increased oxidative stress and undergo premature cellular senescence. The present study demonstrates that G6PD-deficient cells cultured under 3% oxygen concentration had an extended replicative lifespan, as compared wit...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760601184819
更新日期:2007-05-01 00:00:00
abstract::The metabolism of paraquat generates oxygen radicals. Paraquat has thus been suggested as a model compound to induce oxidative damage to DNA, lipids and proteins in different cells and tissues, although experimental data are inconsistent. In order to explore the possibilities for an animal model of oxidative DNA damag...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760000300421
更新日期:2000-05-01 00:00:00
abstract::Recovery of neurological function in patients following cardiac arrest and cardiopulmonary resuscitation (CPR) is a complex event. Free radical induced oxidative stress is supposed to be involved in this process. We studied levels of 8-iso-PGF2alpha (indicating oxidative injury) and 15-keto-dihydro-PGF2alpha (indicati...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576021000043058
更新日期:2003-03-01 00:00:00
abstract::Sonodynamic therapy, a promising new approach to cancer treatment, is based on synergistic cell killing by combination of certain drugs (sonosensitizers) and ultrasound. Although the mechanism of sonodynamic action is not understood, the role of free radicals produced from sonosensitizers by ultrasound is implicated. ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769609145652
更新日期:1996-07-01 00:00:00
abstract::Testing of pregnant women reveals weakening of neutrophil-mediated effector functions, such as reactive oxygen species generation. This study provides data confirming the phenomenon, gained through application of the flow cytometry technique. Key factors influencing neutrophil functional activity in blood plasma of pr...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2016.1197395
更新日期:2016-08-01 00:00:00
abstract::Sublytic levels (microM) of hemin destabilized RBC membrane as indicated by ghost fragmentation pattern using a laser viscodiffractometer. Furthermore, electron microscopic study shows that 5 microM of hemin induced echinocytic transformation whereas higher hemin concentration (40 microM) induced spherocytic transform...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709097838
更新日期:1997-07-01 00:00:00