Abstract:
:Hypoxia is a well-known threat to neuronal cells and triggers the pathophysiological syndromes in extreme environments such as high altitudes and traumatic conditions such as stroke. Among several prophylactic molecules proven suitable for ameliorating free radical damage, NAP (an octapeptide with initial amino acids: asparagine/N, alanine/A, and proline/P) can be considered superlative, primarily due to its high permeability into brain through blood-brain barrier and observed activity at femtomolar concentrations. Several mechanisms of action of NAP have been hypothesized for its protective role during hypoxia, yet any distinct mechanism is unknown. Oxidative stress is advocated as the leading event in hypoxia; we, therefore, investigated the regulation of key antioxidant genes to understand the regulatory role of NAP in providing neuroprotection. Primary neuronal culture of rat was subjected to cellular hypoxia by limiting the oxygen concentration to 0.5% for 72 h and observing the prophylactic efficacies of 15fM NAP by conventional cell death assays using flow cytometry. We performed real-time quantitative polymerase chain reaction to comprehend the regulatory mechanism. Further, we validated the significantly regulated candidates by enzyme assays and immunoblotting. In the present study, we report that NAP regulates a major clad of cellular antioxidants and there is an involvement of more than one route of action in neuroprotection during hypoxia.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Arya A,Meena R,Sethy NK,Das M,Sharma M,Bhargava Kdoi
10.3109/10715762.2015.1011153subject
Has Abstractpub_date
2015-04-01 00:00:00pages
440-52issue
4eissn
1071-5762issn
1029-2470journal_volume
49pub_type
杂志文章abstract::We investigated the antioxidant and radical scavenging activity of polyphenolic isochromans. To assess the relation between structure and scavenging properties the natural occurring 1-(3'-methoxy-4'-hydroxy)phenyl-6,7-dihydroxy-isochroman (ISO-3, three OH groups) was compared with three newly synthesized derivatives t...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500073733
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abstract::An increasing incidence of unresponsiveness to antimonials in Leishmaniasis has led to identification of plant-derived anti-leishmanial compounds like Artemisinin. Since iron-mediated generation of free radicals sustains the anti-malarial activity of Artemisinin, this study investigated whether similar mechanisms acco...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2010.498475
更新日期:2010-11-01 00:00:00
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journal_title:Free radical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1080/10715769900300341
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journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760000301301
更新日期:2000-12-01 00:00:00
abstract::N-nitroso species have recently been detected in animal tissues. Protein N-nitrosotryptophan is the best candidate for this N-nitroso pool. N-nitrosation of N-blocked trytophan derivatives like melatonin (MelH) by N2O3 or peroxynitrite (ONOOH/ONOO- ) has been observed under conditions of pH and reagent concentrations ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600693414
更新日期:2006-09-01 00:00:00
abstract::This work describes the synthesis and characterisation of some novel hybrid molecules which contains in the same molecule a free radical moiety of hydrazyl type and a spin-trap moiety of nitrone type. The new compounds synthesized have multiple and easy to follow spectroscopic properties, making them useful as sensors...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760500385699
更新日期:2006-01-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769509064020
更新日期:1995-07-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2019.1637863
更新日期:2019-08-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章,评审
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更新日期:2016-11-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715762.2019.1702656
更新日期:2020-01-01 00:00:00
abstract::The synproportionation reaction between ferryl leghemoglobin and oxyleghemoglobin does not occur, at least under conditions where this process could be clearly demonstrated with myoglobin and hemoglobin. In contrast, a cross synproportionation can occur between oxyleghemoglobin and ferryl myoglobin or between ferryl l...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709097848
更新日期:1997-08-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760410001725517
更新日期:2004-11-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715760000300291
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abstract::Cerebrovascular diseases (CBD) are one of the most dangerous complications of atherosclerosis. The clinical consequences of CBD deeply impact quality of life and the prognosis of patients. Atherosclerosis is the main cause of CBD development. Hypertension, dyslipidemia, diabetes, smoking, obesity, and other risk facto...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715762.2019.1620939
更新日期:2019-06-01 00:00:00
abstract::The production of reactive oxygen species, including hydrogen peroxide (H(2)O(2)), is increased in diseased blood vessels. Although H(2)O(2) leads to impairment of the nitric oxide (NO)/soluble guanylate cyclase (sGC)/cGMP signaling pathway, it is not clear whether this reactive molecule affects the redox state of sGC...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1089987
更新日期:2015-01-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701534368
更新日期:2007-09-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2016.1211644
更新日期:2016-09-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/1071576021000016535
更新日期:2002-12-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章,多中心研究
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doi:10.1080/10715762.2017.1353688
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journal_title:Free radical research
pub_type: 杂志文章
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760600930014
更新日期:2007-04-01 00:00:00
abstract::Effects of 12 wk exercise training on oxidative stress were examined in elderly humans. We measured oxidative stress during a 45 min cycling test by using antipyrine hydroxylation products. Antipyrine breakdown is independent of blood flow to the liver, which is important during exercise. Furthermore, antipyrine react...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100300951
更新日期:2001-10-01 00:00:00
abstract::Nonalcoholic fatty liver disease (NAFLD) is characterised by excessive accumulation of hepatic lipids and oxidative injury of hepatocytes. Scutellarin is a flavonoid glycoside having antioxidative stress activity. Our current study aims to investigate the molecular mechanism of scutellarin ameliorating NAFLD. Scutella...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1422602
更新日期:2018-02-01 00:00:00
abstract::Soy sauce is a traditional fermented seasoning in Asian countries, that has high antioxidant activity in vitro and some antioxidant activity in vivo. We attempted to identify the major antioxidants present, using the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay as a guide. 3-Hydroxy-2-methyl-4H-p...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760601110871
更新日期:2007-04-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2014.995183
更新日期:2015-03-01 00:00:00
abstract::The effects of various free radicals derived from 6-formylpterin (6-FP), alpha-phenyl-tert-butyl nitrone (PBN) and 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) combined with hyperthermia, on gene expression in similarly enhanced apoptosis of human lymphoma U937 cells were investigated using cDNA microarrays c...
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pub_type: 杂志文章
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更新日期:2007-01-01 00:00:00
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journal_title:Free radical research
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2004-09-01 00:00:00
abstract::Exposure to asbestos fiber is central to mesothelial carcinogenesis. Recent sequencing studies on human and rodent malignant mesothelioma (MM) revealed frequently mutated genes, including CDKN2A, BAP1 and NF2. Crocidolite directly or indirectly catalyses the generation of hydroxyl radicals, which appears to be the maj...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2020.1743285
更新日期:2020-03-01 00:00:00
abstract::Formation of the alpha-hydroxyethyl radical (CH3 degree CHOH) has already been extensively demonstrated after ethanol metabolism in the liver. Despite favourable conditions, this formation in the brain has remained speculative since there is no direct experimental evidence in intact brain cells. In this preliminary st...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709097868
更新日期:1997-12-01 00:00:00