Abstract:
:The present study was designed to determine whether hydroxymethylglutaryl-CoA reductase inhibitors (statins) modulate the NO production via iNOS in adipocytes stimulated by lipopolysaccharide (L) and tumour necrosis factor-alpha (T). Well-differentiated 3T3-L1 adipocytes significantly produced NO by LT-treatment. Pre-incubation with simvastatin, a lipophilic statin, pravastatin, a hydrophilic one, or Y27632, an inhibitor of Rho kinase, further enhanced the production of NO. The effect of simvastatin was offset by mevalonate and geranylgeranyl pyrophosphate (GGPP) but not by squalene. The mRNA level for iNOS parallelled the NO production. The NF-kappaB was activated by the LT-treatment and was further enhanced by simvastatin, pravastatin or Y27632 addition. Mevalonate and GGPP completely offset the effect of simvastatin. Statins and Y27632 also further increased the interleukin-6 secretion in the LT-treated 3T3-L1 adipocytes. These results suggest that statins, especially lipophilic type, enhance induction of iNOS by inhibiting the small GTP-binding protein signal in adipocytes.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Araki S,Dobashi K,Asayama K,Shirahata Adoi
10.1080/10715760701534368subject
Has Abstractpub_date
2007-09-01 00:00:00pages
1028-34issue
9eissn
1071-5762issn
1029-2470pii
781563826journal_volume
41pub_type
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journal_title:Free radical research
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journal_title:Free radical research
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journal_title:Free radical research
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abstract::It has previously been reported that the globular form of adiponectin (gAd), mature adipocyte-derived cytokine, induced generation of reactive oxygen species (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. This study investigated whether diacylglycerol kinases (DGKs), enzymes functioning in sub...
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更新日期:2018-12-01 00:00:00
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更新日期:2004-02-01 00:00:00
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journal_title:Free radical research
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更新日期:1994-11-01 00:00:00
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