Abstract:
:Phospholipids (PLs) are important biomolecules that not only constitute structural building blocks and scaffolds of cell and organelle membranes but also play a vital role in cell biochemistry and physiology. Moreover, dietary exogenous PLs are characterised by high nutritional value and other beneficial health effects, which are confirmed by numerous epidemiological studies. For this reason, PLs are of high interest in lipidomics that targets both the analysis of membrane lipid distribution as well as correlates composition of lipids with their effects on functioning of cells, tissues and organs. Lipidomic assessments follow-up the changes occurring in living organisms, such as free radical attack and oxidative modifications of the polyunsaturated fatty acids (PUFAs) build in PL structures. Oxidised PLs (oxPLs) can be generated exogenously and supplied to organisms with processed food or formed endogenously as a result of oxidative stress. Cellular and tissue oxPLs can be a biomarker predictive of the development of numerous diseases such as atherosclerosis or neuroinflammation. Therefore, suitable high-throughput analytical techniques, which enable comprehensive analysis of PL molecules in terms of the structure of hydrophilic group, fatty acid (FA) composition and oxidative modifications of FAs, have been currently developed. This review addresses all aspects of PL analysis, including lipid isolation, chromatographic separation of PL classes and species, as well as their detection. The bioinformatic tools that enable handling of a large amount of data generated during lipidomic analysis are also discussed. In addition, imaging techniques such as confocal microscopy and mass spectrometry imaging for analysis of cellular lipid maps, including membrane PLs, are presented.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Parchem K,Sasson S,Ferreri C,Bartoszek Adoi
10.1080/10715762.2019.1657573subject
Has Abstractpub_date
2019-01-01 00:00:00pages
1068-1100issue
sup1eissn
1071-5762issn
1029-2470journal_volume
53pub_type
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journal_title:Free radical research
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769709097807
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doi:10.3109/10715762.2015.1066501
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journal_title:Free radical research
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doi:10.3109/10715762.2011.579120
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