A(1) and A(3) adenosine receptors alter glutathione status in an organ-specific manner and influence the changes after inhibition of gamma-glutamylcysteine ligase.

Abstract:

:Adenosine levels are increased in stress and act as anti-oxidant and anti-inflammatory mediators by binding to 4 G-protein-coupled receptors. Using genetically modified mice lacking A(1) and A(3) adenosine receptors, treated with ip buthionine-[S,R]-sulphoximine injections to inhibit gamma-glutamylcysteine ligase, the question was addressed whether these receptors modulate the responses to the stress related to altered glutathione levels. This study determined organ glutathione levels and expression of two sub-units of gamma-glutamylcysteine ligase and the cationic x(c)-transporter and found that deletion of one or both adenosine receptors influenced the responses in an organ-specific manner. The lack of A(1) and A(3) adenosine receptors is related to decreased basal glutathione content and down-regulation of gamma-glutamylcysteine ligase sub-units in several organs. Moreover, responses to buthionine-[S,R]-sulphoximine were different. For example, the lack of A(3) adenosine receptors, or their blockade of A(3) by MRS 1191, caused a marked increase in gene expression, which was not observed in mice lacking both A(1) and A(3) receptors. The results indicate that A(1) and A(3) adenosine receptors play a role in antioxidant responses and their role differs in an organ-specific way.

journal_name

Free Radic Res

journal_title

Free radical research

authors

Conte C,Grottelli S,Prudenzi E,Bellezza I,Fredholm BB,Minelli A

doi

10.1080/10715760802712616

subject

Has Abstract

pub_date

2009-03-01 00:00:00

pages

304-11

issue

3

eissn

1071-5762

issn

1029-2470

pii

908843279

journal_volume

43

pub_type

杂志文章
  • Generation of ROS by CAY10598 leads to inactivation of STAT3 signaling and induction of apoptosis in human colon cancer HCT116 cells.

    abstract::Prostaglandin E2 (PGE2) has been reported to play critical roles in cell fate decision by interacting with four types of prostanoid receptors such as EP1, EP2, EP3 and EP4. The present study was aimed at investigating the effect of the EP4-specific agonist CAY10598 in human colon cancer HCT116 cells. Our study reveale...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715762.2014.951838

    authors: Chae IG,Kim DH,Kundu J,Jeong CH,Kundu JK,Chun KS

    更新日期:2014-11-01 00:00:00

  • Analysis of the oxidative stress response of Penicillium chrysogenum to menadione.

    abstract::The intracellular superoxide and glutathione disulphide concentrations increased in Penicillium chrysogeum treated with 50, 250 or 500 microM menadione (MQ). A significant increase in the intracellular peroxide concentration was also observed when mycelia were exposed to 250 or 500 microM MQ. The specific activity of ...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715769900300131

    authors: Emri T,Pócsi I,Szentirmai A

    更新日期:1999-02-01 00:00:00

  • Effect of MCI-186 on postischemic reperfusion injury in isolated rat heart.

    abstract::MCI-186 (3-methyl-1-phenyl-2-pyrazolin-5-one) is a newly developed antioxidant which has been shown to reduce brain edema in cerebral ischemia through inhibition of the lipoxygenase pathway of arachidonic acid. However, its effect on myocardial reperfusion injury after prolonged ischemia has not yet been demonstrated....

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715769609088034

    authors: Minhaz U,Tanaka M,Tsukamoto H,Watanabe K,Koide S,Shohtsu A,Nakazawa H

    更新日期:1996-05-01 00:00:00

  • A pilot study to assess the effect of acute exercise on brain glutathione.

    abstract::The brain is highly susceptible to oxidative stress due to its high metabolic demand. Increased oxidative stress and depletion of glutathione (GSH) are observed with aging and many neurological diseases. Exercise training has the potential to reduce oxidative stress in the brain. In this study, nine healthy sedentary ...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715762.2017.1411594

    authors: Rai S,Chowdhury A,Reniers RLEP,Wood SJ,Lucas SJE,Aldred S

    更新日期:2018-01-01 00:00:00

  • A spectroscopic study of some of the peptidyl radicals formed following hydroxyl radical attack on beta-amyloid and alpha-synuclein.

    abstract::There is clear evidence implicating oxidative stress in the pathology of many neurodegenerative diseases. Reactive oxygen species (ROS) are the primary mediators of oxidative stress, and hydrogen peroxide, a key ROS, is generated during aggregation of the amyloid proteins associated with some of these diseases. Hydrog...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760600632545

    authors: Tabner BJ,Turnbull S,King JE,Benson FE,El-Agnaf OM,Allsop D

    更新日期:2006-07-01 00:00:00

  • Methods of quantitative analysis of the nitric oxide metabolites nitrite and nitrate in human biological fluids.

    abstract::In human organism, the gaseous radical molecule nitric oxide (NO) is produced in various cells from L-arginine by the catalytic action of NO synthases (NOS). The metabolic fate of NO includes oxidation to nitrate by oxyhaemoglobin in red blood cells and autoxidation in haemoglobin-free media to nitrite. Nitrate and ni...

    journal_title:Free radical research

    pub_type: 杂志文章,评审

    doi:10.1080/10715760500053651

    authors: Tsikas D

    更新日期:2005-08-01 00:00:00

  • 2',7'-Dichlorodihydrofluorescein as a fluorescent probe for reactive oxygen species measurement: Forty years of application and controversy.

    abstract::Reactive oxygen species (ROS) are critically important chemical intermediates in biological studies, due to their multiple physiologically essential functions and their often pathologically deleterious effects. Consequently, it is vital that their presence in biological samples has to be quantifiable. However, their h...

    journal_title:Free radical research

    pub_type: 杂志文章,评审

    doi:10.3109/10715761003709802

    authors: Chen X,Zhong Z,Xu Z,Chen L,Wang Y

    更新日期:2010-06-01 00:00:00

  • Hispidulin: antioxidant properties and effect on mitochondrial energy metabolism.

    abstract::Hispidulin (6-methoxy-5,7,4'-trihydroxyflavone) and eupafolin (6-methoxy-5,7,3',4'-tetrahydroxyflavone), are flavonoids found in the leaves of Eupatorium litoralle. They have recognized antioxidant and antineoplastic properties, although their action mechanisms have not been previously described. We now report the eff...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/13561820500177659

    authors: Dabaghi-Barbosa P,Mariante Rocha A,Franco da Cruz Lima A,Heleno de Oliveira B,Benigna Martinelli de Oliveira M,Gunilla Skare Carnieri E,Cadena SM,Eliane Merlin Rocha M

    更新日期:2005-12-01 00:00:00

  • Neurofilaments are the major neuronal target of hydroxynonenal-mediated protein cross-links.

    abstract::Lipid peroxidation generates reactive aldehydes, most notably hydroxynonenal (HNE), which covalently binds amino acid residue side chains leading to protein inactivation and insolubility. Specific adducts of lipid peroxidation have been demonstrated to be intimately associated with pathological lesions of Alzheimer's ...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715762.2013.794265

    authors: Perry EA,Castellani RJ,Moreira PI,Nunomura A,Lui Q,Harris PL,Sayre LM,Szweda PA,Szweda LI,Zhu X,Smith MA,Perry G

    更新日期:2013-07-01 00:00:00

  • Inhibition of reactive oxygen species and pre-neoplastic lesions by quercetin through an antioxidant defense mechanism.

    abstract::There is a correlation between oxidative stress generated by diethylnitrosamine (DEN) metabolism and liver cancer development. Quercetin is a flavonoid with anti-carcinogenic and antioxidant properties. This study demonstrates the mechanism of action for the chemopreventive effect of quercetin. A 10 mg/kg dose of quer...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760802626535

    authors: Vásquez-Garzón VR,Arellanes-Robledo J,García-Román R,Aparicio-Rautista DI,Villa-Treviño S

    更新日期:2009-02-01 00:00:00

  • Subcutaneous administration of bovine superoxide dismutase protects lungs from radiation-induced lung injury.

    abstract:BACKGROUND:The objective of the present study was to determine whether single administration of the antioxidant enzyme bovine superoxide dismutase (bSOD) after radiation therapy (RT) mitigates development of pulmonary toxicity in rats. METHODS:Female F344 rats (n = 60) were divided among six experimental groups: (1) R...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715762.2015.1066501

    authors: Antonic V,Rabbani ZN,Jackson IL,Vujaskovic Z

    更新日期:2015-10-01 00:00:00

  • Effect of antioxidants on ammonia induced CNS-renal pathobiology in sheep.

    abstract::The effect of antioxidants on ammonia induced CNS and renal pathobiology in 10 sheep infested by L. Cuprina larvae was investigated. The condition produces severe dermatitis, proliferation of macrophages and hyperammonaemia, and free radicals may therefore be involved in the pathogenesis. Five of the sheep (treated gr...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715769409056553

    authors: Guerrini VH

    更新日期:1994-07-01 00:00:00

  • Inhibition of cell growth by overexpression of manganese superoxide dismutase (MnSOD) in human pancreatic carcinoma.

    abstract::Manganese superoxide dismutase (MnSOD) levels have been found to be low in human pancreatic cancer [Pancreas 26, (2003), 23] and human pancreatic cancer cell lines [Cancer Res. 63, (2003), 1297] when compared to normal human pancreas. We hypothesized that stable overexpression of pancreatic cancer cells with MnSOD cDN...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760400017376

    authors: Ough M,Lewis A,Zhang Y,Hinkhouse MM,Ritchie JM,Oberley LW,Cullen JJ

    更新日期:2004-11-01 00:00:00

  • Free radicals and acute pancreatitis: much ado about … something.

    abstract::There is a convincing body of evidence that oxidative stress is involved in the pathogenesis of acute pancreatitis. The effects of different radical scavengers suggested that reactive oxygen metabolites are generated at very early stage of disease and contribute to amplify the pancreatic damage. Oxidative stress is al...

    journal_title:Free radical research

    pub_type: 杂志文章,评审

    doi:10.3109/10715762.2013.829571

    authors: Closa D

    更新日期:2013-11-01 00:00:00

  • Do mitochondria make nitric oxide? no?

    abstract::Several papers have claimed that mitochondria contain nitric oxide synthase (NOS) and make nitric oxide (NO*) in amounts sufficient to affect mitochondrial respiration. However, we found that the addition of L-arginine or the NOS inhibitor L-NMMA to intact rat liver mitochondria did not have any effect on the respirat...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760410001694008

    authors: Tay YM,Lim KS,Sheu FS,Jenner A,Whiteman M,Wong KP,Halliwell B

    更新日期:2004-06-01 00:00:00

  • Dietary docosahexaenoic acid enhances ferric nitrilotriacetate-induced oxidative damage in mice but not when additional alpha-tocopherol is supplemented.

    abstract::Weaning mice were fed a diet supplemented with beef tallow (BT) or BT plus docosahexaenoic acid (DHA) containing 100 mg alpha-tocopherol/kg (alpha-Toc100) or 500 mg alpha-tocopherol/kg (alpha-Toc500) for 4 wk to modify membrane fatty acid unsaturation, and then were administered ferric nitrilotriacetate (Fe-NTA). The ...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715769900300221

    authors: Yasuda S,Watanabe S,Kobayashi T,Hata N,Misawa Y,Utsumi H,Okuyama H

    更新日期:1999-03-01 00:00:00

  • Inactivation of mitochondrial NADP+-dependent isocitrate dehydrogenase by hypochlorous acid.

    abstract::Myeoloperoxidase catalyses the formation of hypochlorous acid (HOCl) via reaction of H(2)O(2) with Cl(-) ion. Although HOCl is known to play a major role in the human immune system by killing bacteria and other invading pathogens, excessive generation of this oxidant is known to cause damage to tissue. Recently, it wa...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760802098834

    authors: Park SY,Lee SM,Shin SW,Park JW

    更新日期:2008-05-01 00:00:00

  • The neurotoxic effect of sickle cell hemoglobin.

    abstract::A growing body of experimental evidence suggests that the oxidative neurotoxicity of hemoglobin A may contribute to neuronal loss after CNS hemorrhage. Several hemoglobin variants, including hemoglobin S, are more potent oxidants in cell-free systems. However, despite the increased incidence of hemorrhagic stroke asso...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760310001638010

    authors: Vanderveldt GM,Regan RF

    更新日期:2004-05-01 00:00:00

  • Oxidative damage of rat cerebral cortex and hippocampus, and changes in antioxidative defense systems caused by hyperoxia.

    abstract::In order to elucidate the oxidative damage in rat brain caused by oxidative stress, regional changes in the levels of lipid peroxidation products and antioxidative defense systems in cerebral cortex and hippocampus, and in their synapses, which modulate learning and memory functions in the brain, were studied. When ra...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/1071576031000090019

    authors: Onodera K,Omoi NO,Fukui K,Hayasaka T,Shinkai T,Suzuki S,Abe K,Urano S

    更新日期:2003-04-01 00:00:00

  • Biomarkers of oxidative stress in overweight men are not influenced by a combination of antioxidants.

    abstract::The effect of antioxidant supplementation on biomarkers of oxidative stress was investigated in a 6-week intervention study in 60 overweight men. The supplement contained a combination of antioxidants aiming to correspond to the antioxidant content found in a diet rich in fruit and vegetables. Placebo, single or doubl...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715761003647101

    authors: Rytter E,Johansson C,Vessby B,Sjödin A,Möller L,Akesson B,Basu S

    更新日期:2010-05-01 00:00:00

  • P53 activation plays a crucial role in silibinin induced ROS generation via PUMA and JNK.

    abstract::Silibinin is an active constituent extracted from blessed milk thistle (Silybum marianum). Our previous study demonstrated that silibinin induced autophagy and apoptosis via reactive oxygen species (ROS) generation in HeLa cells. In this study, we investigated whether the autophagy- and apoptosis-associated molecules ...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715762.2012.655244

    authors: Fan S,Qi M,Yu Y,Li L,Yao G,Tashiro S,Onodera S,Ikejima T

    更新日期:2012-03-01 00:00:00

  • Local and systemic oxidant/antioxidant status before and during lung cancer radiotherapy.

    abstract::To examine local and systemic oxidative status of lung cancer (LC) and oxidant effects of radiotherapy (RT), this study evaluated antioxidants and markers of oxidative and nitrosative stress in bronchoalveolar lavage (BAL) fluid and in the blood of 36 LC patients and 36 non-cancer controls at baseline and during and a...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760902942824

    authors: Crohns M,Saarelainen S,Kankaanranta H,Moilanen E,Alho H,Kellokumpu-Lehtinen P

    更新日期:2009-07-01 00:00:00

  • p47phox and reactive oxygen species production modulate expression of microRNA-451 in macrophages.

    abstract::The production of microRNAs (miRNA) is influenced by various stimuli, including environmental stresses. We hypothesized that reactive oxygen species (ROS)-associated stress could regulate macrophage miRNA synthesis. miRNAs undergo unique steps of maturation processing through either one of two pathways of cytoplasmic ...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715762.2014.974037

    authors: Ranjan R,Lee YG,Karpurapu M,Syed MA,Chung S,Deng J,Jeong JJ,Zhao G,Xiao L,Sadikot RT,Weiss MJ,Christman JW,Park GY

    更新日期:2015-01-01 00:00:00

  • Oxidative damage of mitochondrial DNA in diabetes and its protection by manganese superoxide dismutase.

    abstract::Retinal mitochondria become dysfunctional in diabetes and the production of superoxide radicals is increased; over-expression of MnSOD abrogates mitochondrial dysfunction and prevents the development of diabetic retinopathy. The mitochondrial DNA (mtDNA) is particularly prone to oxidative damage. The aim of this study...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715760903494168

    authors: Madsen-Bouterse SA,Zhong Q,Mohammad G,Ho YS,Kowluru RA

    更新日期:2010-03-01 00:00:00

  • Silver nanoparticles-induced H2O2 triggers apoptosis-like death and is associated with dinF in Escherichia coli.

    abstract::Silver nanoparticles (AgNPs) are the most widely used nanomaterials as antimicrobial agents. AgNPs have been shown to inhibit the growth of and induce apoptosis-like death in Escherichia coli. However, the precise mechanism of AgNPs-induced apoptosis-like death and association with DNA damage-inducible protein F (dinF...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715762.2020.1866178

    authors: Kim S,Lee DG

    更新日期:2021-01-06 00:00:00

  • Suppression of mitochondrial NADP(+)-dependent isocitrate dehydrogenase activity enhances curcumin-induced apoptosis in HCT116 cells.

    abstract::Curcumin is a polyphenol derived from the plant Curcuma longa that induces apoptotic cell death in malignant cancer cell lines. It has been shown previously that mitochondrial NADP(+)-dependent isocitrate dehydrogenase (IDPm) plays an essential role in defense against oxidative stress by supplying NADPH for antioxidan...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715762.2010.540574

    authors: Jung KH,Park JW

    更新日期:2011-04-01 00:00:00

  • Quantum mechanical and experimental oxidation studies of pentadecylresorcinol, olivetol, orcinol and resorcinol.

    abstract::Resorcinols (pentadecylresorcinol, olivetol, orcinol and resorcinol) exhibit antioxidant properties in liposomal systems. Antioxidant potency depends on the length of the alkyl chain. Pentadecylresorcinol has been demonstrated to be the most active antioxidant, indicating significance of its alkyl chain in a lipid bil...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715769809070804

    authors: Hładyszowski J,Zubik L,Kozubek A

    更新日期:1998-04-01 00:00:00

  • Oxidative aggregation of ceruloplasmin induced by hydrogen peroxide is prevented by pyruvate.

    abstract::Ceruloplasmin (CP) is a blue copper glycoprotein with multiple physiological functions including ferroxidase and oxidase activities. CP is also an important serum oxygen free radical (OFR) scavenger and antioxidant, exerting cardioprotective and antifibrillatory actions. Although it has been reported that CP activitie...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760310001624079

    authors: Aouffen M,Paquin J,Furtos A,Waldron KC,Mateescu MA

    更新日期:2004-01-01 00:00:00

  • Involvement of mTOR in globular adiponectin-induced generation of reactive oxygen species.

    abstract::Globular adiponectin (gAd) induces the generation of reactive oxygen species (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. This study investigated the role of the mammalian target of rapamycin (mTOR) in gAd-induced ROS and NO generation. gAd stimulation induced phosphorylation of mTOR, which ...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.3109/10715760903348328

    authors: Fujimoto A,Akifusa S,Kamio N,Hirofuji T,Nonaka K,Yamashita Y

    更新日期:2010-02-01 00:00:00

  • Effect of vegetables on human phenolsulfotransferases in relation to their antioxidant activity and total phenolics.

    abstract::Epidemiology studies have shown that consumption of fruits and vegetables is associated with the prevention of chronic diseases such as cancer and cardiovascular disease. Induction of cellular phase II detoxifying enzymes is associated with cancer preventive potential. Phenolsulfotransferases (PSTs) are traditionally ...

    journal_title:Free radical research

    pub_type: 杂志文章

    doi:10.1080/10715760500150424

    authors: Yeh CT,Yen GC

    更新日期:2005-08-01 00:00:00